FastQCFastQC Report
Mon 15 Aug 2016
SRR2920962_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920962_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2244784
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA33690.15008125503389189No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT31410.1399243757974041No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24970.11123564672592107No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG4300.026.297691
CATGGGG61550.026.1996964
GTACATG125550.024.7923341
TACATGG127350.023.8773782
ACATGGG128900.022.9901123
GTATCAA94300.021.3322891
AACGCAG102550.019.495726
GAGTACT29100.019.21904612-13
TCAACGC104950.018.9940414
CAACGCA105900.018.7666265
ATGGGGA35000.018.7008065
GGTATCA76950.018.6397841
GTATTAG7150.018.3125611
ATCAACG108850.018.2045733
TATCAAC110550.017.9784562
ACGCAGA112950.017.647957
CGCAGAG113600.017.3374548
GTATAGC7000.017.004521
GTATAGA7400.016.8896261
GTACTTT34500.016.6414314-15
Produced by FastQC (version 0.11.5)