FastQCFastQC Report
Mon 15 Aug 2016
SRR2920962_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920962_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2244784
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43530.1939162075282076No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35480.1580552961888538No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT32230.14357728850526377No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA31580.14068168696854574No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26630.11863056757353937No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA113600.027.382941
GGTATCA88700.024.4548871
TACCGTA2052.2689874E-623.2144787
CATGGGG60750.022.94324
GAGTACT64450.022.61894812-13
TCAACGC138050.022.5225964
AACGCAG139500.022.4719036
CAACGCA139150.022.4479275
AGTACTT71150.022.41244112-13
TATCAAC139700.022.3804992
ATCAACG139950.022.1737693
GTATAGG6200.021.1455461
GTACATG130500.020.411971
ACGCAGA153700.020.1644617
TACATGG130900.020.1544512
GTACTTT74650.019.68776314-15
CGCAGAG156850.019.6858438
TATAGGA9700.019.6464982
ACATGGG131500.019.5220873
ACCGTAT2451.0615477E-519.4265238
Produced by FastQC (version 0.11.5)