FastQCFastQC Report
Mon 15 Aug 2016
SRR2920961_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920961_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2748475
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA55050.035.9996641
GGTATCA37900.030.9341751
TCAACGC64400.030.3059844
CAACGCA65200.029.8417845
AACGCAG66850.029.3711746
ATCAACG68150.028.4642723
TATCAAC68550.028.3854982
GTACATG92100.027.9794881
TACATGG92200.027.8165072
ACATGGG95050.026.1055133
CATGGGG65400.025.8393144
ACGCAGA77250.025.2629557
CGCAGAG79500.024.5479668
GCAGAGT89800.020.142169
GTATTAG9800.019.4328041
AGAGTAC76300.019.22159210-11
ATGGGGA37950.018.030625
GTGTAGC20200.017.087881
CAGAGTA84300.017.00929810-11
GTATAAT14350.017.0037021
Produced by FastQC (version 0.11.5)