##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920961_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2748475
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.190536570279882	31.0	27.0	31.0	10.0	34.0
2	27.301447529993904	31.0	27.0	33.0	10.0	34.0
3	27.3613098172623	31.0	27.0	34.0	10.0	34.0
4	30.681407325880716	35.0	31.0	37.0	10.0	37.0
5	30.723347310781435	35.0	31.0	37.0	10.0	37.0
6	30.665928924221614	35.0	31.0	37.0	10.0	37.0
7	30.509336995970493	35.0	30.0	37.0	10.0	37.0
8	30.48199637981062	35.0	30.0	37.0	8.0	37.0
9	31.53812641555772	37.0	30.0	39.0	8.0	39.0
10-11	31.443019310708667	37.0	30.0	39.0	8.0	39.0
12-13	31.36018974158397	37.0	30.0	39.0	7.0	39.0
14-15	32.120355651770524	38.0	31.0	40.0	4.5	41.0
16-17	31.967936946852348	38.0	30.0	40.0	2.0	41.0
18-19	32.06708028997899	38.0	30.0	40.0	2.0	41.0
20-21	32.07074286649869	38.0	30.0	40.0	2.0	41.0
22-23	32.001028206550906	38.0	30.0	40.0	2.0	41.0
24-25	31.90848779777877	38.0	30.0	40.0	2.0	41.0
26-27	31.72888401750061	38.0	30.0	40.0	2.0	41.0
28-29	31.62725838874285	38.0	30.0	40.0	2.0	41.0
30-31	31.4856509518915	37.0	30.0	40.0	2.0	41.0
32-33	31.360160998371825	37.0	30.0	40.0	2.0	41.0
34-35	31.1597311236231	37.0	30.0	40.0	2.0	41.0
36-37	31.005546530348646	37.0	29.0	40.0	2.0	41.0
38-39	30.86375444564713	37.0	29.0	40.0	2.0	41.0
40-41	30.773902618724932	37.0	29.0	40.0	2.0	41.0
42-43	30.56281665287114	37.0	28.0	40.0	2.0	41.0
44-45	30.397924849234577	36.5	28.0	40.0	2.0	41.0
46-47	30.188074841503017	36.0	27.5	40.0	2.0	41.0
48-49	30.021499013089077	36.0	27.0	40.0	2.0	41.0
50-51	29.791321551041943	36.0	27.0	40.0	2.0	41.0
52-53	29.609489262227235	35.5	26.5	40.0	2.0	41.0
54-55	29.38908958604317	35.0	26.0	39.0	2.0	41.0
56-57	29.194177862269076	35.0	26.0	39.0	2.0	41.0
58-59	28.938252849307343	35.0	26.0	39.0	2.0	40.5
60-61	28.73836309226025	35.0	25.5	39.0	2.0	40.0
62-63	28.620260144261817	34.5	26.0	39.0	2.0	40.0
64-65	28.399339269958794	34.0	25.5	38.5	2.0	40.0
66-67	28.140865207069375	34.0	25.0	38.0	2.0	40.0
68-69	27.847022257797505	34.0	24.0	37.5	2.0	40.0
70-71	27.523181946352068	34.0	23.5	37.0	2.0	39.5
72-73	27.249105958758946	34.0	23.0	36.5	2.0	39.0
74-75	26.958350539844822	33.5	23.0	36.0	2.0	39.0
76-77	26.68239187185621	33.0	22.5	35.5	2.0	38.5
78-79	26.364928733206597	33.0	22.0	35.0	2.0	37.0
80-81	26.047188531822194	33.0	20.0	35.0	2.0	37.0
82-83	25.772046680431878	33.0	20.0	35.0	2.0	36.5
84-85	25.528636789492353	32.0	20.0	35.0	2.0	36.0
86-87	25.449393208961332	32.5	19.5	35.0	2.0	36.0
88-89	25.521999472434715	33.0	20.0	35.0	2.0	35.5
90-91	25.42173787282038	33.0	20.0	35.0	2.0	35.0
92-93	25.259390025377712	33.0	18.5	35.0	2.0	35.0
94-95	25.16207842530858	33.0	18.0	35.0	2.0	35.0
96-97	25.05650060488089	33.0	18.0	35.0	2.0	35.0
98-99	24.97721954902264	33.0	17.5	35.0	2.0	35.0
100-101	24.793714878250668	32.5	13.0	35.0	2.0	35.0
102-103	23.56203822119539	30.0	10.0	34.0	2.0	35.0
104-105	24.485936200984185	32.0	10.0	34.5	2.0	35.0
106-107	24.648253486024068	32.5	8.5	35.0	2.0	35.0
108-109	24.62413083619098	33.0	7.0	35.0	2.0	35.0
110-111	24.50348611502742	33.0	3.5	35.0	2.0	35.0
112-113	24.39624810121977	33.0	2.0	35.0	2.0	35.0
114-115	24.24526801226134	32.0	2.0	35.0	2.0	35.0
116-117	24.088024813760356	32.0	2.0	35.0	2.0	35.0
118-119	23.871502014753638	32.0	2.0	35.0	2.0	35.0
120-121	23.62749670271696	32.0	2.0	35.0	2.0	35.0
122-123	23.371373216056178	32.0	2.0	35.0	2.0	35.0
124-125	22.256443846132854	30.5	2.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	236247.0
3	32290.0
4	20258.0
5	17515.0
6	19325.0
7	20740.0
8	21872.0
9	23148.0
10	24163.0
11	24747.0
12	24711.0
13	24650.0
14	24169.0
15	21556.0
16	18655.0
17	16652.0
18	15307.0
19	14860.0
20	15428.0
21	16817.0
22	20222.0
23	26135.0
24	34655.0
25	43072.0
26	49584.0
27	57119.0
28	64934.0
29	74341.0
30	85650.0
31	100109.0
32	118979.0
33	144793.0
34	182049.0
35	239315.0
36	350322.0
37	410810.0
38	113170.0
39	106.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.046387745237915	19.204188771320922	12.903270421206493	24.84615306223467
2	15.562788348378334	20.177672455893024	39.055880360032	25.203658835696647
3	18.598946879231743	24.566776801938108	29.546737374505653	27.287538944324492
4	12.952638535486164	16.361547486651567	36.54515759012012	34.140656387742155
5	13.899996615827664	37.59993770211788	34.50141862320953	13.998647058844934
6	32.91483889935176	36.21333428420896	17.065039893113816	13.806786923325465
7	28.072597951783294	32.55137097202604	21.358113364348398	18.01791771184227
8	25.826312390274076	35.739841539847	20.054797012725935	18.379049057152994
9	26.123724386302783	16.16153934877344	19.550586654043485	38.16414961088029
10-11	24.831643951153097	26.38430733875187	27.95345339117184	20.83059531892319
12-13	25.330024071712636	23.95514740035146	27.736667726130097	22.978160801805807
14-15	22.73957739806622	25.46776272175613	24.948515537392275	26.84414434278537
16-17	22.8863950470353	28.27280965080049	25.875736720207232	22.965058581956978
18-19	22.906211626447394	27.57287222914525	27.254877704909084	22.266038439498267
20-21	23.779293632651502	26.745046814324557	26.885257831268312	22.59040172175563
22-23	23.418510572612774	26.52044583019747	26.671622195164503	23.389421402025253
24-25	23.0563434807097	26.64381239020997	26.935451829191088	23.364392299889243
26-27	23.05825092588988	26.762265243845867	26.860721202632625	23.31876262763163
28-29	23.048482619078026	27.001110105672893	26.845018550880933	23.105388724368147
30-31	23.3737071968008	26.800637380561636	26.693740241042498	23.131915181595065
32-33	23.122076788037003	26.850871847115222	26.531167283675494	23.495884081172285
34-35	23.34357246844298	26.8221130387417	26.627318405445738	23.206996087369586
36-37	23.43660368756071	26.579288304385408	26.641073312569734	23.343034695484146
38-39	23.18391813530415	26.749598635588402	26.706246731097217	23.360236498010234
40-41	23.410429020617467	26.7315239229732	26.738418728255898	23.119628328153436
42-43	23.289681364398128	26.720469974232337	26.595160976944456	23.39468768442508
44-45	23.354943120585233	26.72961375264422	26.581693800787793	23.333749325982755
46-47	23.33334546400738	26.78420076750775	26.439240789634095	23.443212978850777
48-49	23.17720423753851	26.873389055693735	26.479721931546784	23.469684775220966
50-51	23.246339558818498	26.968694758613566	26.40113442928848	23.38383125327946
52-53	23.379935781402075	26.820197021930742	26.348690613715124	23.451176582952055
54-55	23.2649340414609	26.797628061295214	26.3655147647654	23.57192313247848
56-57	23.292918734256016	26.87505650870184	26.253056017883402	23.578968739158743
58-59	23.254594579744932	26.739865562479316	26.42446663177193	23.58107322600382
60-61	23.252566035820536	26.790812935938657	26.527737527840678	23.428883500400136
62-63	23.154473716145542	26.769666974082295	26.54506374128764	23.53079556848452
64-65	23.2169355372791	26.710252161262694	26.43406756952465	23.63874473193356
66-67	23.178828668952907	26.819579537208227	26.348081106171193	23.653510687667673
68-69	23.400288170806697	26.658783206045765	26.376634150423705	23.56429447272383
70-71	23.25256842664072	26.517009532892104	26.487682595208394	23.742739445258778
72-73	23.356617732009934	26.56360956515553	26.4242415862041	23.65553111663044
74-75	23.242623331364936	26.74677060968284	26.326312231460225	23.684293827491995
76-77	23.327386335293895	26.52382956111009	26.4571009321451	23.69168317145092
78-79	23.339840605487396	26.62509434816944	26.390581501264254	23.644483545078916
80-81	23.44627089551085	26.621979207505603	26.342866031315182	23.588883865668368
82-83	23.458387439843083	26.595788806510757	26.426383431746842	23.51944032189932
84-85	23.441458207693994	26.58656675553518	26.255288222329554	23.71668681444127
86-87	23.343435613384646	26.498844002467926	26.408343778582672	23.749376605564763
88-89	23.333139286331512	26.46656782397511	26.422434258998173	23.777858630695203
90-91	23.315716811023186	26.540284446407565	26.42500176917159	23.71899697339766
92-93	23.294597788813658	26.466773985084096	26.49279093867713	23.745837287425115
94-95	23.249500915208618	26.439823009332564	26.590862905005512	23.719813170453307
96-97	23.342376972576723	26.32907021528082	26.558335839391077	23.770216972751378
98-99	23.24354444528128	26.481372935334026	26.346715072184402	23.928367547200292
100-101	23.2142428258577	26.497510547694063	26.46330377722804	23.824942849220196
102-103	22.973692105521216	26.441798188552067	26.59433197968084	23.99017772624587
104-105	22.940048911000684	26.651292073485124	26.383613322917032	24.025045692597164
106-107	22.93428696352616	26.64930345487764	26.369968509315378	24.046441072280828
108-109	22.92630945415273	26.70548161538887	26.37489775006477	23.99331118039363
110-111	22.872149660658618	26.90858261946911	26.32431752797094	23.89495019190133
112-113	22.902936312136912	26.946530458760225	26.26007564774588	23.890457581356983
114-115	22.918889827127042	26.96252645276656	26.280372124538104	23.83821159556829
116-117	22.9186296200547	26.89799361678941	26.26294730549228	23.92042945766361
118-119	22.97720821304087	26.916427446202455	26.12362059740501	23.98274374335166
120-121	22.93102199833858	27.07507278260044	26.137931988066597	23.855973230994383
122-123	22.94734172443417	27.055078015696655	26.114162975501674	23.883417284367496
124-125	23.032080902865378	27.12303947105445	25.915204745423843	23.929674880656332
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	714.0
1	582.5
2	378.5
3	276.5
4	221.0
5	197.0
6	203.5
7	242.0
8	327.0
9	349.5
10	345.0
11	385.0
12	454.5
13	530.0
14	617.5
15	719.5
16	831.0
17	989.5
18	1137.5
19	1324.0
20	1689.5
21	2093.0
22	2596.0
23	3422.5
24	4474.0
25	5892.5
26	8154.0
27	11253.5
28	14689.5
29	18889.5
30	23410.5
31	28688.0
32	35312.0
33	40992.0
34	46313.0
35	51968.5
36	58314.0
37	64251.0
38	69004.0
39	73060.5
40	77763.5
41	83106.0
42	87140.0
43	91716.0
44	97105.0
45	102168.0
46	105559.0
47	107240.5
48	109678.0
49	112198.0
50	113127.0
51	112119.5
52	108633.5
53	105153.0
54	101682.0
55	96607.5
56	89409.5
57	80942.0
58	73240.5
59	65072.5
60	55131.5
61	45301.5
62	36848.0
63	28614.0
64	21346.0
65	16141.0
66	12266.0
67	9279.0
68	7011.0
69	5326.0
70	4110.0
71	3083.5
72	2382.5
73	1921.0
74	1441.5
75	1071.5
76	788.5
77	537.0
78	341.5
79	220.5
80	161.0
81	126.5
82	95.0
83	62.0
84	40.5
85	33.0
86	29.5
87	23.0
88	17.5
89	16.5
90	13.0
91	11.0
92	10.5
93	8.5
94	10.0
95	7.0
96	3.5
97	5.0
98	9.5
99	12.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.034164400258325074
2	0.01808275498230837
3	0.02335840784435005
4	0.021357298138058377
5	0.014116919382566696
6	0.0047662794822583435
7	0.004839047107941677
8	0.005457571926250012
9	0.0033473107814333404
10-11	0.004839047107941677
12-13	0.00831370123432085
14-15	0.0026560183374416723
16-17	0.002201220676920838
18-19	0.0
20-21	0.004275098008895842
22-23	0.0010733224788291688
24-25	0.0017100392035583369
26-27	0.0013825848879833362
28-29	0.0032017755300666733
30-31	0.0014007767944041697
32-33	0.0
34-35	0.007131227316966682
36-37	0.008568387924212518
38-39	0.001364392981562503
40-41	0.0010551305724083356
42-43	0.002510483086075005
44-45	0.0010551305724083356
46-47	0.02255796396183338
48-49	0.0031653917172250064
50-51	0.011242598168075024
52-53	0.0018191906420833373
54-55	2.1830287705000044E-4
56-57	1.6372715778750034E-4
58-59	0.007622408790329183
60-61	8.550196017791684E-4
62-63	0.010751416694712522
64-65	0.011388133419441691
66-67	1.273433449458336E-4
68-69	0.003820300348375008
70-71	0.0054029962069875115
72-73	0.012898061652370861
74-75	0.0016736553907166702
76-77	0.0010733224788291688
78-79	4.1841384767916755E-4
80-81	0.004638936137312509
82-83	0.0013825848879833362
84-85	0.001182473917354169
86-87	0.01511747423571253
88-89	0.0
90-91	4.91181473362501E-4
92-93	0.009714478028725021
94-95	0.007076651597704182
96-97	0.004893622827204177
98-99	0.0010187467595666687
100-101	0.017500613976841703
102-103	0.00842285267284585
104-105	0.007931671199483351
106-107	0.018901390771245873
108-109	0.01116983054239169
110-111	0.02104803572890421
112-113	0.0028561293080708395
114-115	0.015153858048554198
116-117	0.006239823902345847
118-119	0.005039158078570844
120-121	0.008077206450850017
122-123	0.008950417959050019
124-125	0.057340889038466784
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2748475.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.66817965253386
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.68426221577678	46.5229459662876
2	12.386323753205652	15.029114293878559
3	4.687742283313553	8.531903730265377
4	2.2149174638042513	5.37500042438444
5	1.2152651721160201	3.68639628937011
6	0.7251591323355124	2.639645071032387
7	0.4877181381907987	2.0712280139291095
8	0.3198859915880464	1.552552064479403
9	0.23498587577285088	1.2830548794475787
>10	0.9938299923295594	10.062699358483114
>50	0.03672859050661913	1.4913274789926332
>100	0.012334936569407352	1.341328851604133
>500	6.046103506325131E-4	0.24741071020735952
>1k	2.418441402238407E-4	0.16539286763819905
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.6383812841666745E-5	0.0
3	0.0	0.0	0.0	3.6383812841666745E-5	0.0
4	0.0	0.0	0.0	3.6383812841666745E-5	0.0
5	3.6383812841666745E-5	0.0	0.0	3.6383812841666745E-5	0.0
6	3.6383812841666745E-5	0.0	0.0	3.6383812841666745E-5	0.0
7	3.6383812841666745E-5	0.0	0.0	3.6383812841666745E-5	0.0
8	3.6383812841666745E-5	0.0	0.0	3.6383812841666745E-5	0.0
9	3.6383812841666745E-5	0.0	0.0	7.276762568333349E-5	0.0
10-11	3.6383812841666745E-5	0.0	0.0	1.0915143852500023E-4	0.0
12-13	3.6383812841666745E-5	0.0	0.0	1.0915143852500023E-4	0.0
14-15	3.6383812841666745E-5	0.0	0.0	1.4553525136666698E-4	0.0
16-17	3.6383812841666745E-5	0.0	0.0	1.8191906420833372E-4	0.0
18-19	7.276762568333349E-5	0.0	0.0	2.3649478347083383E-4	0.0
20-21	7.276762568333349E-5	0.0	0.0	3.092624091541673E-4	0.0
22-23	7.276762568333349E-5	0.0	0.0	4.3660575410000093E-4	0.0
24-25	7.276762568333349E-5	0.0	0.0	7.458681632541682E-4	0.0
26-27	7.276762568333349E-5	0.0	0.0	0.0012916253558791693	0.0
28-29	7.276762568333349E-5	0.0	0.0	0.004147754663950009	0.0
30-31	7.276762568333349E-5	0.0	0.0	0.015172049954975033	0.0
32-33	7.276762568333349E-5	0.0	0.0	0.04242352577338342	0.0
34-35	9.095953210416686E-5	0.0	0.0	0.07918936864988767	0.0
36-37	1.0915143852500023E-4	0.0	0.0	0.1360754600278336	0.0
38-39	1.0915143852500023E-4	0.0	0.0	0.2244335495138213	0.0
40-41	1.0915143852500023E-4	0.0	0.0	0.31568415212072154	0.0
42-43	1.0915143852500023E-4	0.0	0.0	0.406134310845105	0.0
44-45	1.0915143852500023E-4	0.0	0.0	0.5178326162690219	0.0
46-47	1.4553525136666698E-4	0.0	0.0	0.651233866052993	0.0
48-49	1.4553525136666698E-4	0.0	0.0	0.7847442672754892	0.0
50-51	1.4553525136666698E-4	0.0	0.0	0.9145617114945561	0.0
52-53	1.4553525136666698E-4	0.0	0.0	1.0501277981426063	0.0
54-55	1.8191906420833372E-4	0.0	0.0	1.205759557572836	0.0
56-57	1.8191906420833372E-4	0.0	0.0	1.3825666960769154	1.8191906420833372E-5
58-59	1.8191906420833372E-4	0.0	0.0	1.5709075032518034	3.6383812841666745E-5
60-61	1.8191906420833372E-4	0.0	0.0	1.7565741001828288	3.6383812841666745E-5
62-63	1.8191906420833372E-4	0.0	0.0	1.9509546202894332	3.6383812841666745E-5
64-65	1.8191906420833372E-4	0.0	0.0	2.162799370560038	3.6383812841666745E-5
66-67	1.8191906420833372E-4	0.0	0.0	2.414793658301422	3.6383812841666745E-5
68-69	1.8191906420833372E-4	0.0	0.0	2.6809776330510555	3.6383812841666745E-5
70-71	1.8191906420833372E-4	0.0	0.0	2.9598049827631687	3.6383812841666745E-5
72-73	1.8191906420833372E-4	0.0	0.0	3.2408335531522026	3.6383812841666745E-5
74-75	1.8191906420833372E-4	0.0	0.0	3.542655472580249	3.6383812841666745E-5
76-77	1.8191906420833372E-4	0.0	0.0	3.8844450104148667	3.6383812841666745E-5
78-79	1.8191906420833372E-4	0.0	0.0	4.264055521698396	3.6383812841666745E-5
80-81	1.8191906420833372E-4	0.0	0.0	4.653034864788656	3.6383812841666745E-5
82-83	1.8191906420833372E-4	0.0	0.0	5.054530239496448	3.6383812841666745E-5
84-85	1.8191906420833372E-4	0.0	0.0	5.4739628339351825	3.6383812841666745E-5
86-87	1.8191906420833372E-4	3.6383812841666745E-5	0.0	5.929906584560529	3.6383812841666745E-5
88-89	1.8191906420833372E-4	3.6383812841666745E-5	0.0	6.436478410755055	3.6383812841666745E-5
90-91	1.8191906420833372E-4	3.6383812841666745E-5	0.0	6.96287941494829	3.6383812841666745E-5
92-93	1.8191906420833372E-4	3.6383812841666745E-5	0.0	7.492609538016536	3.6383812841666745E-5
94-95	1.8191906420833372E-4	3.6383812841666745E-5	0.0	8.0401677293772	3.6383812841666745E-5
96-97	1.8191906420833372E-4	3.6383812841666745E-5	0.0	8.628512174933373	3.6383812841666745E-5
98-99	1.8191906420833372E-4	3.6383812841666745E-5	0.0	9.262845759921412	3.6383812841666745E-5
100-101	1.8191906420833372E-4	3.6383812841666745E-5	0.0	9.92772355579003	3.6383812841666745E-5
102-103	1.8191906420833372E-4	3.6383812841666745E-5	0.0	10.595039067119039	3.6383812841666745E-5
104-105	1.8191906420833372E-4	3.6383812841666745E-5	0.0	11.274106549995906	3.6383812841666745E-5
106-107	1.8191906420833372E-4	3.6383812841666745E-5	0.0	11.979806983872875	3.6383812841666745E-5
108-109	1.8191906420833372E-4	3.6383812841666745E-5	0.0	12.730204931825831	3.6383812841666745E-5
110-111	1.8191906420833372E-4	3.6383812841666745E-5	0.0	13.505052802008386	3.6383812841666745E-5
112-113	1.8191906420833372E-4	3.6383812841666745E-5	0.0	14.29816534623746	3.6383812841666745E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5505	0.0	35.999664	1
GGTATCA	3790	0.0	30.934175	1
TCAACGC	6440	0.0	30.305984	4
CAACGCA	6520	0.0	29.841784	5
AACGCAG	6685	0.0	29.371174	6
ATCAACG	6815	0.0	28.464272	3
TATCAAC	6855	0.0	28.385498	2
GTACATG	9210	0.0	27.979488	1
TACATGG	9220	0.0	27.816507	2
ACATGGG	9505	0.0	26.105513	3
CATGGGG	6540	0.0	25.839314	4
ACGCAGA	7725	0.0	25.262955	7
CGCAGAG	7950	0.0	24.547966	8
GCAGAGT	8980	0.0	20.14216	9
GTATTAG	980	0.0	19.432804	1
AGAGTAC	7630	0.0	19.221592	10-11
ATGGGGA	3795	0.0	18.03062	5
GTGTAGC	2020	0.0	17.08788	1
CAGAGTA	8430	0.0	17.009298	10-11
GTATAAT	1435	0.0	17.003702	1
>>END_MODULE