FastQCFastQC Report
Mon 15 Aug 2016
SRR2920961_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920961_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2748475
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31740.11548222195945024No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51300.040.3044051
GTATCAA76900.039.2845761
TCAACGC98100.030.1820414
CAACGCA99300.029.7177755
TATCAAC100350.029.6785492
ATCAACG100750.029.3876383
AACGCAG102150.029.117916
GTACATG101700.025.955131
TACATGG100800.025.3505172
ACGCAGA117200.024.924247
CATGGGG64000.024.4814474
CGCAGAG120050.024.2338648
ACATGGG104050.023.4171813
GAGTACT64600.021.9686312-13
AGAGTAC117700.021.91512510-11
AGTACTT72250.021.74268712-13
GCAGAGT131550.021.4373659
GTATAGG6150.020.346261
CAGAGTA125950.019.4617969
GTACTTT74700.019.15762114-15
Produced by FastQC (version 0.11.5)