FastQCFastQC Report
Mon 15 Aug 2016
SRR2920960_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920960_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2571675
Sequences flagged as poor quality0
Sequence length125
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA67800.034.94211
GTACATG122850.031.252171
TACATGG124850.030.3135992
ACATGGG130550.028.8077373
TCAACGC81200.027.9214424
AACGCAG82200.027.8670046
CAACGCA82200.027.652015
CATGGGG82100.026.890554
ATCAACG84650.026.8537753
TATCAAC87450.026.6744332
GGTATCA46750.025.0830441
ACGCAGA93250.024.0543617
CGCAGAG97600.022.8603468
GTGTACG3757.2759576E-1222.2224731
GTATAGA9200.020.7041681
GCAGAGT103700.019.8517469
AGAGTAC92900.018.41438710-11
TAAGGTG10450.018.220865
GTATAGG5750.017.5985411
ATGGGGA44600.016.9435335
Produced by FastQC (version 0.11.5)