FastQCFastQC Report
Mon 15 Aug 2016
SRR2920960_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920960_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2571675
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33630.13077080113155823No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26040.1012569628743912No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56350.039.7501871
GTATCAA84700.039.738391
TCAACGC111000.029.8877284
TATCAAC114950.029.2717972
ATCAACG113900.029.1784843
CAACGCA113250.029.105555
AACGCAG116350.028.3773426
GTACATG128400.025.981741
CATGGGG79200.025.7194464
TACATGG129700.025.2541452
ACGCAGA133100.024.5399237
CGCAGAG135000.024.1064058
ACATGGG134900.024.018133
AGTACTT65150.022.33070812-13
GAGTACT63400.021.72700112-13
GCAGAGT144550.021.5671169
AGAGTAC133350.021.0145210-11
GTACCGT4351.8189894E-1220.5138476
GTACTTT67700.020.39094514-15
CAGAGTA138450.019.2090610-11
Produced by FastQC (version 0.11.5)