##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920960_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2571675
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.90208132831715	31.0	30.0	34.0	26.0	34.0
2	30.049520643160587	31.0	30.0	34.0	26.0	34.0
3	30.165633682327666	31.0	30.0	34.0	26.0	34.0
4	33.717652891597886	35.0	35.0	37.0	28.0	37.0
5	33.25227526806459	35.0	33.0	37.0	27.0	37.0
6	33.05201473747655	35.0	33.0	37.0	26.0	37.0
7	32.888627450980394	35.0	33.0	37.0	26.0	37.0
8	32.82691514285437	35.0	33.0	37.0	26.0	37.0
9	34.13646825512555	38.0	34.0	39.0	25.0	39.0
10-11	34.03446469713319	37.5	33.5	39.0	25.0	39.0
12-13	33.93307688568734	37.5	33.0	39.0	25.0	39.0
14-15	34.810360173816676	38.0	33.0	41.0	25.0	41.0
16-17	34.80240174205528	38.0	33.5	41.0	25.0	41.0
18-19	34.840533310002236	38.5	33.5	41.0	24.5	41.0
20-21	34.805192530160305	39.0	34.0	41.0	24.0	41.0
22-23	34.76606725188836	39.0	34.0	41.0	24.0	41.0
24-25	34.72988480270641	39.0	34.0	41.0	24.0	41.0
26-27	34.58130809686294	39.0	33.5	41.0	23.0	41.0
28-29	34.453006503543406	38.5	33.0	41.0	22.5	41.0
30-31	34.281578932019016	38.0	33.0	40.5	22.0	41.0
32-33	34.13086976386985	38.0	33.0	40.0	21.0	41.0
34-35	33.99010411502231	38.0	33.0	40.0	19.5	41.0
36-37	33.837616145119426	38.0	33.0	40.0	18.5	41.0
38-39	33.68710451359522	38.0	33.0	40.0	17.5	41.0
40-41	33.49031914997035	38.0	32.5	40.0	15.5	41.0
42-43	33.31161538685876	38.0	32.0	40.0	15.0	41.0
44-45	33.07063742502454	38.0	32.0	40.0	11.0	41.0
46-47	32.89275958276221	38.0	32.0	40.0	9.0	41.0
48-49	32.65818756258081	38.0	31.0	40.0	8.0	41.0
50-51	32.44595176295605	37.0	31.0	40.0	6.5	41.0
52-53	32.20017245569522	37.0	31.0	40.0	2.0	41.0
54-55	32.00064821565711	37.0	31.0	40.0	2.0	41.0
56-57	31.859311343774	37.0	30.5	40.0	2.0	41.0
58-59	31.77844012171056	36.0	31.0	40.0	2.0	41.0
60-61	31.496830664839063	36.0	30.0	39.5	2.0	41.0
62-63	31.236407788698028	35.5	30.0	39.0	2.0	41.0
64-65	30.947380403822415	35.0	30.0	39.0	2.0	41.0
66-67	30.630517269872747	35.0	29.0	38.5	2.0	40.5
68-69	30.319253793733658	35.0	29.0	38.0	2.0	40.0
70-71	29.97474369817337	35.0	29.0	37.0	2.0	40.0
72-73	29.7001429029718	35.0	29.0	37.0	2.0	39.0
74-75	29.43661310235547	34.0	29.0	36.0	2.0	39.0
76-77	29.12272954397426	34.0	29.0	36.0	2.0	38.5
78-79	28.81795580701294	34.0	28.5	35.0	2.0	37.0
80-81	28.575266120330134	34.0	28.0	35.0	2.0	37.0
82-83	28.338001885930375	34.0	28.0	35.0	2.0	36.5
84-85	28.155486988052534	34.0	27.5	35.0	2.0	36.0
86-87	27.93849164455073	34.0	27.0	35.0	2.0	36.0
88-89	27.8238574081095	34.0	27.0	35.0	2.0	35.0
90-91	27.648511378770646	34.0	27.0	35.0	2.0	35.0
92-93	27.797929753954136	34.0	27.0	35.0	2.0	35.0
94-95	27.743763306016508	34.0	27.0	35.0	2.0	35.0
96-97	27.68792693477986	34.0	27.0	35.0	2.0	35.0
98-99	27.64075864951831	34.0	27.0	35.0	2.0	35.0
100-101	27.51069225310352	34.0	27.0	35.0	2.0	35.0
102-103	26.205646514431255	31.5	24.5	34.0	2.0	35.0
104-105	27.26437010897567	33.5	26.5	35.0	2.0	35.0
106-107	27.46175197099167	34.0	27.0	35.0	2.0	35.0
108-109	27.44062838422429	34.0	27.0	35.0	2.0	35.0
110-111	27.383079510435806	34.0	27.0	35.0	2.0	35.0
112-113	27.28368378585941	34.0	27.0	35.0	2.0	35.0
114-115	27.19236528721553	34.0	27.0	35.0	2.0	35.0
116-117	27.0659087948516	34.0	26.0	35.0	2.0	35.0
118-119	26.939563708477937	34.0	25.5	35.0	2.0	35.0
120-121	26.80656925933487	34.0	25.0	35.0	2.0	35.0
122-123	26.57235848231211	34.0	25.0	35.0	2.0	35.0
124-125	25.46715720299027	32.5	21.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	84425.0
3	27372.0
4	14079.0
5	10902.0
6	12456.0
7	14657.0
8	16399.0
9	17566.0
10	18807.0
11	19436.0
12	20174.0
13	22101.0
14	23613.0
15	19665.0
16	14647.0
17	13422.0
18	12225.0
19	11610.0
20	11713.0
21	12178.0
22	14089.0
23	18241.0
24	24982.0
25	33156.0
26	40452.0
27	47090.0
28	55272.0
29	64876.0
30	76606.0
31	90334.0
32	110410.0
33	136057.0
34	176498.0
35	243238.0
36	386712.0
37	508714.0
38	147201.0
39	300.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.94630180559235	19.276121293042376	13.920346342577409	24.85723055878786
2	16.242331342019522	20.642003306024222	37.88485221076274	25.23081314119352
3	19.060207156045212	24.42774659082248	29.22400477837041	27.288041474761897
4	13.644587706835726	16.45209104645961	34.809529501406125	35.09379174529854
5	14.376715368182136	37.39820576912082	33.47733643015182	14.74774243254522
6	33.74885723473945	35.45272947368053	16.48529541575657	14.313117875823462
7	29.306895271130777	31.791588325306382	20.499038515756094	18.402477887806747
8	26.900380340517543	35.23598223522	19.154384027253847	18.70925339700861
9	27.083730384381717	15.459981532750586	18.93951003446857	38.51677804839913
10-11	26.03029582926743	25.683723303589524	26.646763994260997	21.639216872882052
12-13	26.526782130622685	22.88740008525229	27.298894831657844	23.286922952467183
14-15	23.792855853725325	24.15951105897289	24.814095522889644	27.233537564412135
16-17	23.88975991712816	27.29254664993203	25.314576694863085	23.503116738076717
18-19	23.94560318614689	25.91169824811325	27.299218848620093	22.843479717119763
20-21	24.67986813569764	25.227351574872976	26.935881838632692	23.156898450796692
22-23	24.381740269362822	24.947050203855014	26.837762913653567	23.833446613128594
24-25	23.896645222742034	25.24764942343321	26.96298837337515	23.892716980449606
26-27	23.69884149161212	25.48432589140039	26.95265661176648	23.864176005221008
28-29	23.85472587590081	25.5299081773819	27.100176457799286	23.515189488918008
30-31	24.138655286503603	25.28679726186867	26.79701294056628	23.77753451106145
32-33	23.84283883464181	25.429911067635658	26.80467745730838	23.92257264041415
34-35	24.07797125378873	25.527786934583457	26.757936519512747	23.63630529211507
36-37	24.169990258231735	25.398359360975785	26.652672801640364	23.778977579152112
38-39	24.20040651378932	25.49729480262532	26.593435060968318	23.70886362261704
40-41	24.134357778493072	25.36668712056161	26.69062138673518	23.808333714210143
42-43	24.28116022586203	25.254997206189444	26.490127920154393	23.973714647794132
44-45	24.266672008435847	25.380975431686586	26.376197595153666	23.976154964723904
46-47	24.132515858638236	25.634569205591827	26.20231759599888	24.030597339771056
48-49	24.40976964917491	25.678635325153515	25.996722082417044	23.91487294325453
50-51	24.105778441914346	25.870243410287696	25.9257840348708	24.098194112927157
52-53	24.488684973776646	25.74458977426348	25.696493692567437	24.07023155939244
54-55	24.103005837726148	25.641360258652345	25.886919728153647	24.368714175467858
56-57	24.096560334651222	25.73189396488185	25.874054612247598	24.297491088219335
58-59	24.11522689768576	25.588313945064574	26.013802411775515	24.282656745474153
60-61	24.337367864045774	25.519406846765957	26.065232617826066	24.077992671362196
62-63	24.044855127113063	25.781096662262048	26.086670659546918	24.08737755107797
64-65	23.897542807526406	25.415373830612282	26.168259565171386	24.518823796689926
66-67	24.015136809732184	25.813372067466787	25.887146120861853	24.28434500193917
68-69	24.00909321459022	25.5439864515848	26.090987378876424	24.355932954948564
70-71	23.778399819196522	25.47387977113267	26.12678338241764	24.620937027253166
72-73	24.105682707583533	25.623435236490323	25.90404236084205	24.366839695084096
74-75	23.972439436575893	25.62035576696816	25.84786849029488	24.559336306161065
76-77	24.052185188656754	25.488662470621133	25.888211155237478	24.57094118548463
78-79	24.00473070899955	25.686462361651465	25.836684349451662	24.472122579897327
80-81	23.966810518092664	25.70197337241456	25.828263917290002	24.502952192202773
82-83	24.01443953444949	25.73568883425655	25.69698372440406	24.552887906889897
84-85	23.93040677271297	25.67572140151007	25.507196999308867	24.886674826468095
86-87	23.78370293899761	25.585886712264212	25.860292734765544	24.770117613972634
88-89	23.81983088651161	25.607452569571244	25.735952525616362	24.836764018300787
90-91	23.775243827060848	25.712420797292022	25.85325370500825	24.659081670638876
92-93	23.76431926416524	25.657931584916753	25.76641026957478	24.81133888134322
94-95	23.53960306988331	25.663065429975212	25.88227057795133	24.915060922190147
96-97	23.799638852276736	25.439621319927646	25.798843907255897	24.961895920539725
98-99	23.579158383666616	25.38512790015204	25.689616881023124	25.346096835158217
100-101	23.400351800766806	25.567125589373248	25.93265611523663	25.099866494623324
102-103	23.559280303324968	25.39821514975659	25.844349919018956	25.198154627899488
104-105	23.500745317335994	25.47867070003432	25.674077055991663	25.346506926638018
106-107	23.385174511874276	25.605769810388846	25.70735207471663	25.301703603020243
108-109	23.353622478773403	25.530390256698755	25.716136958634994	25.39985030589285
110-111	23.11721532865949	25.75034950742255	25.731602802437926	25.40083236148003
112-113	23.25921273561104	25.827646519514385	25.574649336103576	25.338491408770995
114-115	23.094908679017806	25.955148928309264	25.59198456026407	25.35795783240886
116-117	23.028952008124943	25.850222539479876	25.619681399767398	25.501144052627783
118-119	23.149812773407408	25.724863823715733	25.51185444352539	25.613468959351472
120-121	22.996278494598567	25.85573236270873	25.603626620624215	25.544362522068486
122-123	22.85004053904289	25.946056824655518	25.4988829889621	25.705019647339494
124-125	22.780524998039862	26.027020143997703	25.389367158351757	25.80308769961068
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	31.0
1	45.0
2	127.0
3	215.5
4	185.0
5	159.5
6	237.0
7	506.5
8	804.0
9	854.5
10	690.0
11	542.5
12	567.5
13	631.5
14	694.5
15	832.0
16	1009.0
17	1166.0
18	1358.0
19	1565.5
20	1776.5
21	2154.0
22	2692.5
23	3257.5
24	3918.5
25	5030.5
26	6980.5
27	9580.5
28	11949.5
29	14686.0
30	18000.5
31	21795.0
32	26219.0
33	30555.0
34	34542.5
35	37654.5
36	41717.0
37	47213.0
38	51269.5
39	53255.0
40	56368.0
41	61908.5
42	67645.0
43	74173.5
44	80072.0
45	84435.5
46	90212.5
47	95738.5
48	102030.5
49	108691.0
50	114508.5
51	115339.0
52	111087.5
53	110341.5
54	109359.5
55	103621.0
56	95995.0
57	89197.5
58	84187.0
59	75521.5
60	63978.0
61	52768.0
62	42894.5
63	34798.5
64	25990.0
65	18899.5
66	14289.5
67	11121.5
68	8726.0
69	6832.5
70	5519.5
71	4508.5
72	3718.0
73	3367.5
74	2581.5
75	1677.0
76	1182.0
77	720.0
78	404.0
79	239.5
80	180.0
81	130.5
82	83.5
83	54.5
84	31.0
85	23.5
86	18.5
87	12.0
88	7.0
89	4.5
90	3.5
91	2.5
92	2.0
93	2.5
94	3.0
95	5.5
96	5.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04946192656537082
2	2.3331097436495667E-4
3	0.0033830091282918725
4	0.12723225135368973
5	0.016876160479065202
6	0.002410880068437886
7	0.005055071111240728
8	0.011471122906277038
9	0.02476984844507957
10-11	0.019287040547503086
12-13	0.018781533436379012
14-15	0.02842505371013056
16-17	0.03727142815480183
18-19	0.04069332244548786
20-21	0.02127018382960522
22-23	0.023622736154451866
24-25	0.021406281897984776
26-27	0.020375825094539553
28-29	0.020298054769751234
30-31	0.025333683299794883
32-33	0.02410880068437886
34-35	0.028288955641750997
36-37	0.030583180223006406
38-39	0.030738720872583045
40-41	0.03314960094102093
42-43	0.03163307960764871
44-45	0.028833347915269227
46-47	0.03865185141979449
48-49	0.03248855318032022
50-51	0.02270893483818912
52-53	0.03031098408624729
54-55	0.03153586670166331
56-57	0.02488650393226205
58-59	0.029066658889634188
60-61	0.04178210699252433
62-63	0.04924805817220294
64-65	0.04273479347118123
66-67	0.038418540445429535
68-69	0.02904721630843711
70-71	0.03608543070177997
72-73	0.038846277231765286
74-75	0.023719949060437263
76-77	0.020375825094539553
78-79	0.03161363702645163
80-81	0.02408935810318178
82-83	0.03713533008642227
84-85	0.020764676718481145
86-87	0.03303294545383845
88-89	0.033324584171794645
90-91	0.03491887582995518
92-93	0.025780862667327712
94-95	0.022067329658685488
96-97	0.01664284950470024
98-99	0.025119814906627003
100-101	0.024011587778393458
102-103	0.02733626916309409
104-105	0.024206013590364256
106-107	0.013998658461897401
108-109	0.0167789475730798
110-111	0.021639592872349733
112-113	0.011762761624233233
114-115	0.015068000427736787
116-117	0.012968201658452175
118-119	0.007465951179678613
120-121	0.005249496923211526
122-123	0.003110812991532756
124-125	0.06624087413845062
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2571675.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.23983048736061
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.25894554408792	31.082438410911493
2	13.645780333464405	12.073740176404486
3	5.647463041054544	7.49528422859661
4	2.9912308438198325	5.29326181916616
5	1.8214742856782997	4.029085681774712
6	1.1800529207977006	3.132320470932303
7	0.8149661479068753	2.5237774965436186
8	0.5964957289293735	2.111109594741607
9	0.42862971212777107	1.706625522574089
>10	2.37880002469392	19.21662852088866
>50	0.15954419168195066	4.86405641797297
>100	0.07430753996739987	5.508663443255306
>500	0.0018655154457617499	0.5008790285655209
>1k	4.44170344227359E-4	0.46212918767232813
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3363	0.13077080113155823	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2604	0.1012569628743912	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.888516239415945E-5	0.0
2	3.888516239415945E-5	0.0	0.0	3.888516239415945E-5	0.0
3	3.888516239415945E-5	0.0	0.0	3.888516239415945E-5	0.0
4	3.888516239415945E-5	0.0	0.0	7.77703247883189E-5	0.0
5	3.888516239415945E-5	0.0	0.0	7.77703247883189E-5	0.0
6	7.77703247883189E-5	0.0	0.0	7.77703247883189E-5	0.0
7	1.1665548718247835E-4	0.0	0.0	7.77703247883189E-5	0.0
8	1.1665548718247835E-4	0.0	0.0	7.77703247883189E-5	0.0
9	1.1665548718247835E-4	0.0	0.0	7.77703247883189E-5	0.0
10-11	1.1665548718247835E-4	0.0	0.0	7.77703247883189E-5	0.0
12-13	1.7498323077371752E-4	0.0	0.0	1.1665548718247835E-4	0.0
14-15	2.333109743649567E-4	0.0	0.0	1.1665548718247835E-4	0.0
16-17	2.721961367591161E-4	0.0	0.0	1.1665548718247835E-4	0.0
18-19	3.6940904274451476E-4	0.0	0.0	1.5554064957663778E-4	0.0
20-21	4.2773678633575396E-4	0.0	0.0	2.527535555620364E-4	0.0
22-23	4.2773678633575396E-4	0.0	0.0	3.4996646154743504E-4	0.0
24-25	7.388180854890295E-4	0.0	0.0	7.77703247883189E-4	0.0
26-27	7.388180854890295E-4	0.0	0.0	0.0015748490769634579	0.0
28-29	8.360309914744281E-4	0.0	0.0	0.003655205265050988	0.0
30-31	8.554735726715079E-4	0.0	0.0	0.010090699641284378	0.0
32-33	8.749161538685877E-4	0.0	0.0	0.02403103035959054	0.0
34-35	9.915716410510659E-4	0.0	0.0	0.04236538442843672	3.888516239415945E-5
36-37	0.0010887845470364645	0.0	0.0	0.07518446148910729	3.888516239415945E-5
38-39	0.0013220955214014213	0.0	0.0	0.12855434687509115	3.888516239415945E-5
40-41	0.0013220955214014213	0.0	0.0	0.18600717431246172	3.888516239415945E-5
42-43	0.001477636170978059	0.0	0.0	0.24038807392069372	3.888516239415945E-5
44-45	0.0015554064957663779	0.0	0.0	0.3078149455121662	3.888516239415945E-5
46-47	0.0018664877949196535	0.0	0.0	0.3885405426424412	3.888516239415945E-5
48-49	0.0019442581197079724	0.0	0.0	0.47471006250789854	3.888516239415945E-5
50-51	0.0019442581197079724	0.0	0.0	0.565059737330728	3.888516239415945E-5
52-53	0.0019442581197079724	0.0	0.0	0.6534651540338495	3.888516239415945E-5
54-55	0.0019831432821021317	0.0	0.0	0.751125239386781	3.888516239415945E-5
56-57	0.002060913606890451	0.0	0.0	0.8682667910991864	3.888516239415945E-5
58-59	0.002060913606890451	0.0	0.0	0.998182118658073	3.888516239415945E-5
60-61	0.0021581265128758492	0.0	0.0	1.1357578232086092	3.888516239415945E-5
62-63	0.0022164542564670885	0.0	0.0	1.2767359794686342	3.888516239415945E-5
64-65	0.0022164542564670885	0.0	0.0	1.4218942906860315	3.888516239415945E-5
66-67	0.0023331097436495667	0.0	0.0	1.5903253715963332	3.888516239415945E-5
68-69	0.0023331097436495667	0.0	0.0	1.7865204584560646	3.888516239415945E-5
70-71	0.0024497652308320453	0.0	0.0	1.9966753186152992	3.888516239415945E-5
72-73	0.002508092974423284	0.0	0.0	2.2044776264496875	3.888516239415945E-5
74-75	0.002527535555620364	0.0	0.0	2.4195903448141776	3.888516239415945E-5
76-77	0.002527535555620364	0.0	0.0	2.6633225427007687	3.888516239415945E-5
78-79	0.0025469781368174437	0.0	0.0	2.937637920810367	3.888516239415945E-5
80-81	0.0029941575043502776	0.0	0.0	3.229587720065716	3.888516239415945E-5
82-83	0.003033042666744437	0.0	0.0	3.539405251441181	3.888516239415945E-5
84-85	0.0030719278291385967	0.0	0.0	3.851925301603041	3.888516239415945E-5
86-87	0.0031108129915327558	0.0	0.0	4.187543138227031	3.888516239415945E-5
88-89	0.0031108129915327558	0.0	0.0	4.554307989928743	3.888516239415945E-5
90-91	0.0034996646154743503	0.0	0.0	4.9551945716313295	3.888516239415945E-5
92-93	0.00353854977786851	0.0	0.0	5.374901571932689	3.888516239415945E-5
94-95	0.00353854977786851	0.0	0.0	5.803357733772735	3.888516239415945E-5
96-97	0.00353854977786851	0.0	0.0	6.246862453459321	3.888516239415945E-5
98-99	0.0036940904274451476	0.0	0.0	6.723166807625381	3.888516239415945E-5
100-101	0.003927401401810105	0.0	0.0	7.247902631553365	3.888516239415945E-5
102-103	0.004082942051386742	0.0	0.0	7.7891646494988676	3.888516239415945E-5
104-105	0.004432908512934177	0.0	0.0	8.335112329512867	3.888516239415945E-5
106-107	0.004607891743707895	0.0	0.0	8.909932242604528	3.888516239415945E-5
108-109	0.004666219487299133	0.0	0.0	9.501647758756452	3.888516239415945E-5
110-111	0.004880087880467011	0.0	0.0	10.129604246259733	3.888516239415945E-5
112-113	0.00495785820525533	0.0	0.0	10.779316982122548	3.888516239415945E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5635	0.0	39.750187	1
GTATCAA	8470	0.0	39.73839	1
TCAACGC	11100	0.0	29.887728	4
TATCAAC	11495	0.0	29.271797	2
ATCAACG	11390	0.0	29.178484	3
CAACGCA	11325	0.0	29.10555	5
AACGCAG	11635	0.0	28.377342	6
GTACATG	12840	0.0	25.98174	1
CATGGGG	7920	0.0	25.719446	4
TACATGG	12970	0.0	25.254145	2
ACGCAGA	13310	0.0	24.539923	7
CGCAGAG	13500	0.0	24.106405	8
ACATGGG	13490	0.0	24.01813	3
AGTACTT	6515	0.0	22.330708	12-13
GAGTACT	6340	0.0	21.727001	12-13
GCAGAGT	14455	0.0	21.567116	9
AGAGTAC	13335	0.0	21.01452	10-11
GTACCGT	435	1.8189894E-12	20.513847	6
GTACTTT	6770	0.0	20.390945	14-15
CAGAGTA	13845	0.0	19.20906	10-11
>>END_MODULE