FastQCFastQC Report
Mon 15 Aug 2016
SRR2920959_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920959_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2771043
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA29940.10804595958994502No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT29620.10689115975464833No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG84350.030.4050374
GTATCAA94250.030.246161
GTACATG156350.028.5101851
TACATGG155800.027.9966182
ACATGGG157350.027.0401043
GTATAGG6050.025.5760861
TCAACGC108100.025.1561364
CAACGCA110500.024.500735
AACGCAG111400.024.3023476
TATCAAC115650.024.080712
ATCAACG112700.024.0774233
ATGGGGA42650.023.7175
GTATAAT17200.022.144591
ACGCAGA122400.021.7780327
CGCAGAG123800.021.2433828
GGTATCA71300.020.5334381
ATGGGGG42800.019.0461255
GCAGAGT134650.019.0455259
GTATATA10750.018.2692871
ATGGGGC37100.017.8024165
Produced by FastQC (version 0.11.5)