FastQCFastQC Report
Mon 15 Aug 2016
SRR2920959_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920959_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2771043
Sequences flagged as poor quality0
Sequence length125
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41490.14972701614518433No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32750.11818654564364392No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT31480.11360343379731026No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA106050.036.3489381
GGTATCA74000.033.4129261
CATGGGG84850.029.4079064
TCAACGC136000.028.1124084
CAACGCA137500.027.4757445
ATCAACG140100.027.374723
TATCAAC143050.026.8503682
AACGCAG142550.026.749426
GTACATG153150.026.687341
TACATGG152200.026.48822
ACATGGG155550.025.191723
ACGCAGA156950.023.8808527
CGCAGAG161950.023.1439788
ATGGGGA40800.021.2897785
AGTACTT86200.021.08809312-13
GCAGAGT172700.020.9454489
GAGTACT79900.020.0326712-13
ATGGGGC36600.019.9941275
AGAGTAC161750.019.89990410-11
ACCGTCG2701.1074608E-619.8315738
Produced by FastQC (version 0.11.5)