##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920959_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2771043
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.585414228505297	31.0	30.0	34.0	26.0	34.0
2	29.608892030906773	31.0	30.0	34.0	26.0	34.0
3	29.742959239535438	31.0	30.0	34.0	26.0	34.0
4	33.273308642269356	35.0	33.0	37.0	28.0	37.0
5	32.76297661205546	35.0	33.0	37.0	26.0	37.0
6	32.51299853520858	35.0	33.0	37.0	25.0	37.0
7	32.32959394711666	35.0	32.0	37.0	23.0	37.0
8	32.241827716134324	35.0	32.0	37.0	23.0	37.0
9	33.48431583342445	37.0	33.0	39.0	22.0	39.0
10-11	33.38344767656078	37.0	33.0	39.0	21.5	39.0
12-13	33.25658641890436	37.0	32.5	39.0	20.0	39.0
14-15	34.07059796618096	38.0	33.0	40.0	18.0	41.0
16-17	34.05158653257997	38.0	33.0	40.0	18.5	41.0
18-19	34.093215442705144	38.0	33.0	40.0	18.5	41.0
20-21	34.07067627604479	38.0	33.0	40.0	18.0	41.0
22-23	34.036450535051245	38.0	33.0	40.0	18.0	41.0
24-25	34.00466304564743	38.0	33.0	40.0	18.0	41.0
26-27	33.83861997089183	38.0	32.5	40.0	18.0	41.0
28-29	33.70420830712479	38.0	32.0	40.0	17.0	41.0
30-31	33.50953359439028	38.0	32.0	40.0	16.0	41.0
32-33	33.348161324093496	38.0	32.0	40.0	15.5	41.0
34-35	33.19337177373285	38.0	32.0	40.0	14.0	41.0
36-37	33.02077232291235	38.0	32.0	40.0	9.0	41.0
38-39	32.862852001935735	38.0	31.0	40.0	9.0	41.0
40-41	32.64703488902915	38.0	31.0	40.0	8.0	41.0
42-43	32.4672273219867	38.0	31.0	40.0	7.5	41.0
44-45	32.20710974171097	37.5	30.5	40.0	2.0	41.0
46-47	32.02529805564187	37.0	30.5	40.0	2.0	41.0
48-49	31.782891135215152	37.0	30.0	40.0	2.0	41.0
50-51	31.559901632706527	37.0	30.0	40.0	2.0	41.0
52-53	31.30344476790869	36.5	30.0	40.0	2.0	41.0
54-55	31.09915526392048	36.0	29.0	40.0	2.0	41.0
56-57	30.94381916845029	36.0	29.0	40.0	2.0	41.0
58-59	30.86218510503085	36.0	29.0	39.5	2.0	41.0
60-61	30.58204979136015	35.0	29.0	39.0	2.0	41.0
62-63	30.31821447736466	35.0	28.5	39.0	2.0	41.0
64-65	30.044649613881848	35.0	28.0	39.0	2.0	40.5
66-67	29.729738224921086	35.0	28.0	38.0	2.0	40.0
68-69	29.41637679386426	35.0	28.0	37.5	2.0	40.0
70-71	29.072219557762185	34.0	28.0	37.0	2.0	39.5
72-73	28.801429100883674	34.0	28.0	36.5	2.0	39.0
74-75	28.553325769394412	34.0	27.0	36.0	2.0	39.0
76-77	28.241077096241376	34.0	27.0	35.5	2.0	38.5
78-79	27.933098295479354	34.0	26.0	35.0	2.0	37.0
80-81	27.69117188004661	34.0	26.0	35.0	2.0	37.0
82-83	27.451912330483502	34.0	26.0	35.0	2.0	36.0
84-85	27.264511413211558	34.0	26.0	35.0	2.0	36.0
86-87	27.04800827702782	33.0	25.5	35.0	2.0	36.0
88-89	26.923121005339866	33.0	25.0	35.0	2.0	35.0
90-91	26.737258317536032	33.0	25.0	35.0	2.0	35.0
92-93	26.8819071014055	33.0	25.0	35.0	2.0	35.0
94-95	26.824404384919326	33.5	25.0	35.0	2.0	35.0
96-97	26.760032052912926	33.0	25.0	35.0	2.0	35.0
98-99	26.703277249757583	33.5	25.0	35.0	2.0	35.0
100-101	26.563186677363	33.0	25.0	35.0	2.0	35.0
102-103	25.268123591008873	31.5	21.5	34.0	2.0	35.0
104-105	26.31988605012625	33.0	24.5	35.0	2.0	35.0
106-107	26.510295762281565	33.5	25.0	35.0	2.0	35.0
108-109	26.48262170597858	34.0	25.0	35.0	2.0	35.0
110-111	26.411916379500425	34.0	25.0	35.0	2.0	35.0
112-113	26.29419590385281	33.5	24.0	35.0	2.0	35.0
114-115	26.188194842158712	33.0	24.0	35.0	2.0	35.0
116-117	26.04567738573526	33.0	24.0	35.0	2.0	35.0
118-119	25.90141834681021	33.0	23.0	35.0	2.0	35.0
120-121	25.746201881385456	33.0	22.5	35.0	2.0	35.0
122-123	25.490671743455444	33.0	18.5	35.0	2.0	35.0
124-125	24.372673394097458	32.0	8.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	115901.0
3	35624.0
4	17644.0
5	14114.0
6	15773.0
7	18661.0
8	20632.0
9	21637.0
10	23275.0
11	23911.0
12	24815.0
13	26227.0
14	27713.0
15	22666.0
16	17495.0
17	15614.0
18	14816.0
19	14232.0
20	14006.0
21	14556.0
22	16621.0
23	21288.0
24	28655.0
25	37505.0
26	45193.0
27	53007.0
28	61936.0
29	72636.0
30	85130.0
31	100693.0
32	121922.0
33	149598.0
34	190259.0
35	261660.0
36	404258.0
37	489413.0
38	131670.0
39	287.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.63486068938626	19.287217795364857	13.11754660235187	24.960374912897016
2	16.320532710317472	21.054320097494188	37.30762165932826	25.31752553286008
3	18.874105863973316	25.10069574889091	29.68551457232862	26.33968381480716
4	13.44077552037981	16.546470161036822	35.091393906109424	34.92136041247394
5	13.67975586559614	37.96077030107143	33.98264999882697	14.376823834505458
6	33.269661331835906	36.0830062519578	16.444698827703778	14.20263358850252
7	28.332252449672147	32.44520425092236	21.108124094827602	18.114419204577896
8	27.13085156104788	34.7823952850056	19.683549671837977	18.403203482108538
9	26.989979219179723	14.96179353964473	19.18213737432513	38.86608986685042
10-11	25.70093584443921	25.78870002355177	27.23786474787425	21.272499384134772
12-13	26.174323076140105	23.169444192786095	27.595324472633386	23.06090825844041
14-15	23.509141774208103	24.428445086183554	25.110008122010647	26.95240501759769
16-17	23.237328143453816	27.33060373685044	26.07854891315237	23.35351920654338
18-19	23.34090686327158	26.119490410402047	27.98899765321055	22.550605073115822
20-21	24.37275442293407	25.327221681996328	27.567871743394385	22.732152151675216
22-23	23.79854630234406	25.090461302559735	27.56219490911637	23.54879748597983
24-25	23.48023731893278	25.471863946963108	27.665714113488466	23.382184620615647
26-27	23.218164942205853	25.56043096510911	27.734404820537385	23.486999272147653
28-29	23.350092754634886	25.613832830141618	27.818991030981437	23.217083384242056
30-31	23.59723703180171	25.579149812623225	27.534260964483238	23.289352191091826
32-33	23.19015856021964	25.81692245106876	27.34800118540251	23.64491780330909
34-35	23.447382627449258	25.771992739282148	27.4911158724151	23.28950876085349
36-37	23.782579549856127	25.33648523327537	27.44049937531158	23.440435841556923
38-39	23.627704521301172	25.587363402180944	27.309022964605862	23.47590911191202
40-41	23.551998665745156	25.66951453303517	27.409803776814833	23.36868302440484
42-43	23.7516645976141	25.479344550137196	27.14435729836484	23.62463355388387
44-45	23.61979893796186	25.65339567210705	27.025984576423806	23.700820813507278
46-47	23.74928970760141	25.738000119856203	26.875153881644167	23.63755629089822
48-49	23.70568661115114	25.947491765806692	26.59718441849484	23.749637204547327
50-51	23.505779654115926	26.14459675253609	26.626745952220755	23.722877641127226
52-53	23.859941834955016	25.91281091162238	26.45500601847398	23.772241234948627
54-55	23.621027803077148	26.01287434196063	26.35355851158109	24.012539343381125
56-57	23.603729128332947	26.16708523554313	26.28468759841934	23.944498037704587
58-59	23.369950226702507	25.857911196438028	26.591217685123837	24.18092089173563
60-61	23.426833464083813	26.062048292596106	26.656292070455795	23.85482617286429
62-63	23.196945208320884	26.113074734405384	26.70897357692572	23.98100648034801
64-65	23.136099822951596	26.017974167684766	26.633058518396567	24.212867490967067
66-67	23.22033463864088	26.224732154428942	26.455348120721755	24.09958508620842
68-69	23.635635441233987	25.793212962142402	26.470050481249523	24.101101115374085
70-71	23.261445600456305	25.84480119564197	26.6548558514978	24.238897352403917
72-73	23.504009068690273	25.881670198594207	26.394076470142274	24.22024426257324
74-75	23.376659472715506	26.04782443438326	26.280102787231947	24.29541330566929
76-77	23.289433695026794	25.87190478493665	26.44496308589901	24.393698434137548
78-79	23.432259242954807	26.12668126125707	26.27517210372014	24.165887392067987
80-81	23.394551212866983	26.106130643543953	26.135639252116498	24.36367889147257
82-83	23.331344184483267	26.008662725191684	26.235068297118914	24.424924793206134
84-85	23.249063332442987	26.09221934263624	26.061827277780587	24.596890047140185
86-87	23.213403536904075	25.953270500042507	26.179486952709336	24.653839010344083
88-89	23.179985585688353	26.027669485934673	25.986372284524002	24.80597264385297
90-91	23.052478175156843	26.025234258550974	26.14253951900331	24.779748047288873
92-93	23.143069186131296	25.998547462318488	26.10055719114443	24.757826160405784
94-95	22.86864229285785	25.967492965484084	26.3147113651175	24.84915337654057
96-97	23.107676204398004	25.68856645281214	26.253286772601353	24.9504705701885
98-99	23.051433563025878	25.83232564603284	26.01629024281955	25.09995054812173
100-101	22.800567787532678	25.96752637928965	26.203539016442555	25.028366816735115
102-103	22.829697248738	25.750272397263775	26.19695517885089	25.223075175147336
104-105	22.748356950709876	25.942969968948333	25.918948015301137	25.389725065040658
106-107	22.62361332478662	26.009742819029142	26.001170841851174	25.365473014333066
108-109	22.61666263920679	25.99422464058304	26.020825727329477	25.36828699288069
110-111	22.507435125048524	26.19192557782278	25.939061843239646	25.36157745388905
112-113	22.42638891670984	26.43379717027524	25.821405362953353	25.318408550061566
114-115	22.368437911739182	26.488604349788435	25.70456181521926	25.438395923253125
116-117	22.29686101988792	26.357132209925588	25.82430214709257	25.52170462309392
118-119	22.284484018670238	26.26138333428973	25.696443697134463	25.75768894990557
120-121	22.130294111809043	26.54424488926823	25.740314015197313	25.585146983725416
122-123	22.06783457063596	26.555109137778526	25.586000020931472	25.79105627065404
124-125	21.995614411236062	26.77668253410838	25.53154726063198	25.69615579402357
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	141.0
1	126.0
2	239.0
3	448.0
4	387.5
5	282.0
6	408.5
7	777.5
8	1169.0
9	1208.0
10	957.5
11	750.5
12	758.5
13	825.0
14	922.0
15	1079.5
16	1227.5
17	1377.5
18	1607.5
19	1841.5
20	2156.0
21	2634.0
22	3205.0
23	3899.5
24	4869.0
25	6355.0
26	8842.0
27	12258.5
28	14890.5
29	17540.5
30	21242.5
31	25754.5
32	31808.5
33	36570.5
34	39769.0
35	43670.5
36	49626.5
37	55291.5
38	60029.5
39	64291.0
40	68424.0
41	73154.5
42	76898.0
43	82938.0
44	90064.5
45	96204.0
46	102581.5
47	106620.5
48	110558.0
49	116729.0
50	123111.0
51	123234.0
52	118146.0
53	115563.5
54	113375.5
55	107382.5
56	97834.0
57	89045.5
58	82168.5
59	74350.5
60	63907.0
61	53350.0
62	43010.0
63	31392.0
64	22173.5
65	16880.5
66	12860.0
67	9435.5
68	6884.0
69	4964.5
70	3808.0
71	2975.5
72	2359.5
73	2242.5
74	1815.0
75	1271.0
76	892.5
77	539.0
78	296.5
79	156.0
80	93.0
81	60.5
82	35.0
83	17.0
84	10.0
85	8.0
86	8.5
87	7.0
88	7.5
89	6.5
90	2.5
91	2.5
92	2.5
93	3.5
94	3.0
95	2.5
96	6.0
97	4.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04810463063907706
2	2.165249691181263E-4
3	0.002598299629417515
4	0.12100137024217957
5	0.01580632274562322
6	0.0025622121345644944
7	0.0058461741661894094
8	0.011114948414730482
9	0.025261246397114735
10-11	0.019866165916588085
12-13	0.019198547261807197
14-15	0.028617383418445688
16-17	0.03740468841515632
18-19	0.03928123814751341
20-21	0.021544234427253565
22-23	0.02379970285556738
24-25	0.0214359719426945
26-27	0.020335303349677362
28-29	0.02026312835997132
30-31	0.024864283953731504
32-33	0.024395146520642227
34-35	0.0286354271658722
36-37	0.03110742056330414
38-39	0.03128785803756925
40-41	0.034553776321767656
42-43	0.030818720604479974
44-45	0.02899630211440241
46-47	0.03814448205964325
48-49	0.03200960793462967
50-51	0.022915559231668364
52-53	0.02968196451660981
54-55	0.03182917046036456
56-57	0.024918415196011037
58-59	0.02823846472248897
60-61	0.04099539415303191
62-63	0.04913312424238815
64-65	0.042547156431711815
66-67	0.038613619492732516
68-69	0.029104564598961474
70-71	0.035474007440519685
72-73	0.03872188197729158
74-75	0.02381774660299389
76-77	0.020100734633132722
78-79	0.03025936443425815
80-81	0.023998184077258997
82-83	0.03659271978096334
84-85	0.02103900949931127
86-87	0.03278548907396962
88-89	0.03173895172323201
90-91	0.03320049526477936
92-93	0.025946908799322134
94-95	0.021526190679827053
96-97	0.01688894759121385
98-99	0.024286884036083166
100-101	0.02311404045335998
102-103	0.026434089979837917
104-105	0.0238357903504204
106-107	0.013965860508119144
108-109	0.017303953782023592
110-111	0.020876615772472677
112-113	0.012612579451130857
114-115	0.01452521667834097
116-117	0.013208023116205702
118-119	0.007794898888252546
120-121	0.005701824186777326
122-123	0.003464399505890021
124-125	0.06299072226594823
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2771043.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.146817210815904
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.89131174774015	28.34658211897598
2	12.651256168265917	10.411178500856897
3	6.036165653917017	7.451070144477856
4	3.385547748343296	5.572180574382839
5	2.109217125364125	4.3393785757640115
6	1.426087297898291	3.5207372023972408
7	1.0204547193878082	2.9391924667395206
8	0.7517802775967064	2.4746692531974497
9	0.5609654681544123	2.0773749221756432
>10	2.9264365224067594	21.287047936976794
>50	0.16433440743152994	4.626744442710169
>100	0.0716547474205559	5.01283752495799
>500	0.003724090279320371	1.0473940321630648
>1k	0.0010640257940866093	0.8936123042245319
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4149	0.14972701614518433	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3275	0.11818654564364392	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	3148	0.11360343379731026	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	3.608749485302105E-5	0.0
10-11	0.0	0.0	0.0	3.608749485302105E-5	3.608749485302105E-5
12-13	0.0	0.0	0.0	3.608749485302105E-5	1.6239372683859472E-4
14-15	0.0	0.0	0.0	3.608749485302105E-5	1.9848122169161575E-4
16-17	0.0	0.0	0.0	3.608749485302105E-5	2.1652496911812627E-4
18-19	0.0	0.0	0.0	7.21749897060421E-5	2.1652496911812627E-4
20-21	0.0	0.0	0.0	2.8869995882416835E-4	2.706562113976579E-4
22-23	0.0	0.0	0.0	6.856624022073998E-4	3.247874536771894E-4
24-25	0.0	0.0	0.0	0.0019487247220631365	3.247874536771894E-4
26-27	0.0	0.0	0.0	0.0028148245985356417	4.5109368566276307E-4
28-29	0.0	0.0	0.0	0.006585967810676341	4.691374330892736E-4
30-31	0.0	0.0	0.0	0.019126372272101157	4.691374330892736E-4
32-33	0.0	0.0	0.0	0.04395456873097964	5.052249279422947E-4
34-35	0.0	0.0	0.0	0.07897748248583655	5.052249279422947E-4
36-37	0.0	0.0	0.0	0.13624833681758097	5.052249279422947E-4
38-39	0.0	0.0	0.0	0.22843384241962322	5.413124227953157E-4
40-41	0.0	0.0	0.0	0.33191473391066106	5.413124227953157E-4
42-43	0.0	0.0	0.0	0.4231800083939513	5.593561702218262E-4
44-45	0.0	0.0	0.0	0.5279600496996979	5.954436650748473E-4
46-47	0.0	0.0	0.0	0.6615739993930083	6.315311599278683E-4
48-49	0.0	0.0	0.0	0.7993380109944161	6.856624022073998E-4
50-51	0.0	0.0	0.0	0.944734527757238	7.217498970604209E-4
52-53	0.0	0.0	0.0	1.0886875447259388	7.93924886766463E-4
54-55	0.0	0.0	0.0	1.2453253161354767	7.93924886766463E-4
56-57	0.0	0.0	0.0	1.4279460838391898	8.119686341929735E-4
58-59	0.0	0.0	0.0	1.6265175242679382	8.30012381619484E-4
60-61	0.0	0.0	0.0	1.8359152131525929	8.660998764725051E-4
62-63	0.0	0.0	0.0	2.0464496581251175	9.743623610315682E-4
64-65	0.0	0.0	0.0	2.266980339171929	9.924061084580788E-4
66-67	0.0	0.0	1.8043747426510525E-5	2.52184827157139	0.0010104498558845894
68-69	0.0	0.0	3.608749485302105E-5	2.8101873554470282	0.0010104498558845894
70-71	3.608749485302105E-5	0.0	3.608749485302105E-5	3.1269633852668473	0.0010284936033111
72-73	3.608749485302105E-5	0.0	3.608749485302105E-5	3.4386330345649636	0.0010826248455906313
74-75	3.608749485302105E-5	0.0	3.608749485302105E-5	3.7506455150641838	0.001136756087870163
76-77	3.608749485302105E-5	0.0	3.608749485302105E-5	4.094667603498033	0.0011908873301496945
78-79	3.608749485302105E-5	0.0	3.608749485302105E-5	4.489933934623172	0.0011908873301496945
80-81	3.608749485302105E-5	0.0	3.608749485302105E-5	4.910263030923735	0.001208931077576205
82-83	3.608749485302105E-5	0.0	3.608749485302105E-5	5.351414611754491	0.0012630623198557366
84-85	3.608749485302105E-5	0.0	3.608749485302105E-5	5.790328767904359	0.0012630623198557366
86-87	3.608749485302105E-5	0.0	3.608749485302105E-5	6.248694083780006	0.0013171935621352682
88-89	3.608749485302105E-5	0.0	3.608749485302105E-5	6.751447018324869	0.0013893685518413103
90-91	3.608749485302105E-5	0.0	3.608749485302105E-5	7.3019437085602785	0.0014074122992678208
92-93	3.608749485302105E-5	0.0	3.608749485302105E-5	7.877827951424789	0.0014434997941208418
94-95	3.608749485302105E-5	0.0	3.608749485302105E-5	8.45717659379519	0.0014434997941208418
96-97	3.608749485302105E-5	0.0	3.608749485302105E-5	9.046214728533624	0.0014795872889738627
98-99	3.608749485302105E-5	0.0	3.608749485302105E-5	9.676013688708547	0.0015156747838268839
100-101	3.608749485302105E-5	0.0	3.608749485302105E-5	10.373476701732887	0.0015156747838268839
102-103	3.608749485302105E-5	0.0	3.608749485302105E-5	11.100802116748099	0.0015698060261064156
104-105	3.608749485302105E-5	0.0	3.608749485302105E-5	11.821379170225796	0.0016239372683859471
106-107	3.608749485302105E-5	0.0	3.608749485302105E-5	12.537409199352012	0.0016239372683859471
108-109	3.608749485302105E-5	0.0	3.608749485302105E-5	13.278177206199976	0.0016239372683859471
110-111	3.608749485302105E-5	0.0	3.608749485302105E-5	14.06548003766091	0.0017321997529450102
112-113	3.608749485302105E-5	0.0	3.608749485302105E-5	14.897693756466428	0.0017682872477980313
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	10605	0.0	36.348938	1
GGTATCA	7400	0.0	33.412926	1
CATGGGG	8485	0.0	29.407906	4
TCAACGC	13600	0.0	28.112408	4
CAACGCA	13750	0.0	27.475744	5
ATCAACG	14010	0.0	27.37472	3
TATCAAC	14305	0.0	26.850368	2
AACGCAG	14255	0.0	26.74942	6
GTACATG	15315	0.0	26.68734	1
TACATGG	15220	0.0	26.4882	2
ACATGGG	15555	0.0	25.19172	3
ACGCAGA	15695	0.0	23.880852	7
CGCAGAG	16195	0.0	23.143978	8
ATGGGGA	4080	0.0	21.289778	5
AGTACTT	8620	0.0	21.088093	12-13
GCAGAGT	17270	0.0	20.945448	9
GAGTACT	7990	0.0	20.03267	12-13
ATGGGGC	3660	0.0	19.994127	5
AGAGTAC	16175	0.0	19.899904	10-11
ACCGTCG	270	1.1074608E-6	19.831573	8
>>END_MODULE