FastQCFastQC Report
Mon 15 Aug 2016
SRR2920958_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920958_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3405370
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG146800.030.0015051
TACATGG148200.028.9085272
GTATCAA89050.028.13751
CATGGGG87050.027.4764544
ACATGGG156400.027.0500553
TCAACGC99050.024.508074
AACGCAG102350.024.1825736
CAACGCA101050.024.1404045
ATCAACG101450.023.7540843
TATCAAC108050.022.9092162
ACGCAGA115800.020.9113887
ATGGGGA46750.020.3627015
CGCAGAG118250.020.176248
GGTATCA67650.018.9150731
GTATAGC15400.017.3911861
GTATATA18500.016.7289621
GCAGAGT133550.016.6173579
ATGGGGG46250.016.466275
ACGGCGT3307.517254E-616.2265288
TACGGCG2955.2363E-516.1348517
Produced by FastQC (version 0.11.5)