##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920958_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3405370
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.042811207005407	31.0	27.0	31.0	10.0	34.0
2	27.15980025665346	31.0	27.0	33.0	10.0	34.0
3	27.235977588338418	31.0	27.0	34.0	10.0	34.0
4	30.550171053365712	35.0	30.0	37.0	10.0	37.0
5	30.584949653047982	35.0	30.0	37.0	10.0	37.0
6	30.52209422177326	35.0	30.0	37.0	10.0	37.0
7	30.355912573376756	35.0	30.0	37.0	8.0	37.0
8	30.325306501202512	35.0	30.0	37.0	8.0	37.0
9	31.368772262632255	37.0	30.0	39.0	8.0	39.0
10-11	31.273528867641403	37.0	30.0	39.0	7.0	39.0
12-13	31.18792275141908	37.0	30.0	39.0	2.0	39.0
14-15	31.937952997765294	38.0	30.0	40.0	2.0	41.0
16-17	31.781722397272542	38.0	30.0	40.0	2.0	41.0
18-19	31.867065399648205	38.0	30.0	40.0	2.0	41.0
20-21	31.864906750220975	38.0	30.0	40.0	2.0	41.0
22-23	31.798690744324404	38.0	30.0	40.0	2.0	41.0
24-25	31.704542825008737	38.0	30.0	40.0	2.0	41.0
26-27	31.528483395343237	37.5	30.0	40.0	2.0	41.0
28-29	31.42163612177238	37.0	30.0	40.0	2.0	41.0
30-31	31.275030026105828	37.0	30.0	40.0	2.0	41.0
32-33	31.151202659329236	37.0	29.5	40.0	2.0	41.0
34-35	30.95439834731615	37.0	29.0	40.0	2.0	41.0
36-37	30.800043901250085	37.0	29.0	40.0	2.0	41.0
38-39	30.654792871259218	37.0	28.0	40.0	2.0	41.0
40-41	30.55828823299671	37.0	28.0	40.0	2.0	41.0
42-43	30.340893794213258	36.5	28.0	40.0	2.0	41.0
44-45	30.167618349841575	36.0	27.0	40.0	2.0	41.0
46-47	29.95588687866517	36.0	27.0	40.0	2.0	41.0
48-49	29.77960060140308	36.0	27.0	40.0	2.0	41.0
50-51	29.543611854218483	35.5	26.0	40.0	2.0	41.0
52-53	29.365161788586846	35.0	26.0	39.5	2.0	41.0
54-55	29.14920493221001	35.0	26.0	39.0	2.0	41.0
56-57	28.949888705192095	35.0	26.0	39.0	2.0	41.0
58-59	28.694419549123886	35.0	25.0	39.0	2.0	40.0
60-61	28.504291310489023	34.5	24.5	39.0	2.0	40.0
62-63	28.385709335549443	34.0	25.0	39.0	2.0	40.0
64-65	28.17193491456141	34.0	24.0	38.5	2.0	40.0
66-67	27.922641445716618	34.0	23.5	38.0	2.0	40.0
68-69	27.631120406886772	34.0	23.0	37.5	2.0	40.0
70-71	27.31533093320256	34.0	22.5	37.0	2.0	40.0
72-73	27.04279065123614	34.0	22.0	36.5	2.0	39.0
74-75	26.749110669325212	33.0	22.0	36.0	2.0	39.0
76-77	26.474990089182675	33.0	21.5	36.0	2.0	38.5
78-79	26.16126147819473	33.0	20.0	35.0	2.0	37.5
80-81	25.842111136234827	33.0	20.0	35.0	2.0	37.0
82-83	25.57129489600249	32.5	19.0	35.0	2.0	36.5
84-85	25.31714879734067	32.0	18.0	35.0	2.0	36.0
86-87	25.229882949576698	32.0	18.0	35.0	2.0	36.0
88-89	25.30007223884629	33.0	18.5	35.0	2.0	35.5
90-91	25.198868111247823	33.0	18.0	35.0	2.0	35.0
92-93	25.035982139973044	33.0	17.5	35.0	2.0	35.0
94-95	24.938390982477674	32.5	16.5	35.0	2.0	35.0
96-97	24.82995239871145	32.0	15.0	35.0	2.0	35.0
98-99	24.754249171162016	32.0	12.0	35.0	2.0	35.0
100-101	24.574998458317303	32.0	8.5	35.0	2.0	35.0
102-103	23.354294687508258	30.0	7.0	34.0	2.0	35.0
104-105	24.26582706137659	32.0	7.0	34.5	2.0	35.0
106-107	24.433737890449496	32.0	6.0	35.0	2.0	35.0
108-109	24.414016979065416	32.0	2.0	35.0	2.0	35.0
110-111	24.295583152491503	32.0	2.0	35.0	2.0	35.0
112-113	24.190806872674628	32.0	2.0	35.0	2.0	35.0
114-115	24.04274190469758	32.0	2.0	35.0	2.0	35.0
116-117	23.895814551722722	32.0	2.0	35.0	2.0	35.0
118-119	23.68604248583854	32.0	2.0	35.0	2.0	35.0
120-121	23.45013155692333	32.0	2.0	35.0	2.0	35.0
122-123	23.192253998831255	32.0	2.0	35.0	2.0	35.0
124-125	22.097043346244313	30.5	2.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	305440.0
3	41977.0
4	25561.0
5	22045.0
6	23956.0
7	26025.0
8	28004.0
9	29158.0
10	30116.0
11	31135.0
12	31049.0
13	30861.0
14	30518.0
15	27214.0
16	23203.0
17	20618.0
18	19442.0
19	18745.0
20	18859.0
21	20807.0
22	25141.0
23	32800.0
24	43143.0
25	53232.0
26	61698.0
27	71250.0
28	81110.0
29	93198.0
30	106456.0
31	125267.0
32	148001.0
33	179511.0
34	225506.0
35	297392.0
36	424038.0
37	493641.0
38	139158.0
39	95.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.64096551817331	18.856034459207184	12.70020836387212	24.80279165874739
2	15.481324423796112	20.04745735431858	39.385257204586196	25.085961017299113
3	18.891591475777904	24.731411458230838	29.082932081912567	27.29406498407869
4	12.71808949184682	16.164315402909516	36.77813960605917	34.33945549918449
5	13.884985160938834	37.74878005442213	34.38656158173672	13.979673202902317
6	32.94613983133971	36.173421261909766	17.230796067647365	13.649642839103157
7	28.3350243819352	32.40522908446971	21.280987670273422	17.97875886332167
8	26.033722499335564	35.82942870274247	19.92138367088626	18.215465127035706
9	26.45252705010344	16.12584079606373	19.39772498682184	38.02390716701099
10-11	24.96065929977151	26.36136002456252	27.753382815664978	20.924597860000997
12-13	25.465929987047364	23.83931037530926	27.710989858296315	22.983769779347057
14-15	22.760365749981574	25.35999274067866	24.976103265608053	26.903538243731713
16-17	23.021573754990392	28.19802689634819	25.84259219832643	22.937807150334994
18-19	23.037036960904526	27.492487230089786	27.207701604099526	22.262774204906165
20-21	23.912418482226943	26.666703864251524	26.778678383514343	22.642199270007186
22-23	23.380925832961736	26.442138486582884	26.625909033579237	23.551026646876146
24-25	23.037761353087987	26.633031911660243	26.93403267311875	23.395174062133016
26-27	23.029664547683005	26.74108437441039	26.764253952079496	23.464997125827107
28-29	23.084787542199074	26.903671583725075	26.80708650780786	23.204454366267992
30-31	23.291008878606288	26.80125115976602	26.64465796978581	23.263081991841883
32-33	23.022259405330317	26.80170242906093	26.54935095883134	23.626687206777415
34-35	23.255515159346405	26.760666165466528	26.774600899810874	23.209217775376192
36-37	23.441238172754545	26.513704605234274	26.702009580000908	23.343047642010276
38-39	23.345375738456873	26.692821762370873	26.586635206158586	23.375167293013668
40-41	23.34029496078799	26.710404911823627	26.697381226041262	23.25191890134712
42-43	23.37813954123498	26.65267277176761	26.597376526464895	23.37181116053252
44-45	23.33450844901322	26.70282264156706	26.557418594362943	23.405250315056787
46-47	23.29155645796439	26.840326846788475	26.483263134104195	23.384853561142936
48-49	23.359149155604424	26.85642808394973	26.40226519083916	23.382157569606694
50-51	23.207471505136425	27.030330155840886	26.398047484562237	23.36415085446045
52-53	23.434960770137497	26.83193091738383	26.29882295729557	23.434285355183103
54-55	23.25373925004155	26.917108959341178	26.309067875896897	23.520083914720374
56-57	23.308961978807552	26.87013781786617	26.235565419731927	23.58533478359435
58-59	23.343200740055504	26.66541855718544	26.4155470390438	23.575833663715255
60-61	23.33017456988889	26.70344908189811	26.547194539401488	23.419181808811512
62-63	23.14994368650047	26.840452916187594	26.62439116076293	23.385212236549002
64-65	23.162227307802155	26.772524065341134	26.469298197311204	23.595950429545507
66-67	23.182971708197176	26.902070495758462	26.382258833700206	23.53269896234416
68-69	23.511930064284723	26.661545150321935	26.35100917218353	23.47551561320981
70-71	23.202653582784603	26.65671622067084	26.47922336597696	23.661406830567593
72-73	23.387240893563586	26.677553612038547	26.408786737016243	23.526418757381617
74-75	23.34392607098216	26.802557938379074	26.34976649040817	23.503749500230594
76-77	23.308520836609436	26.5839201689229	26.480934872738864	23.626624121728796
78-79	23.46986464428656	26.630646006494764	26.392580168825003	23.506909180393677
80-81	23.389802286715096	26.701160625084796	26.346676909721918	23.56236017847819
82-83	23.358783131686298	26.753533393151297	26.38226263727922	23.505420837883186
84-85	23.439050185466144	26.846714357471363	26.21094789842478	23.50328755863771
86-87	23.297622937278582	26.6418252904789	26.360072688558894	23.70047908368362
88-89	23.288893717863257	26.568610753016557	26.396309358454438	23.74618617066574
90-91	23.275015912426227	26.638921170537895	26.400458688992273	23.685604228043605
92-93	23.292202135074966	26.670713561900683	26.425256737764858	23.61182756525949
94-95	23.125489791270546	26.591872104293806	26.588906008385244	23.693732096050407
96-97	23.331376531950827	26.400711026639417	26.56990729225186	23.698005149157893
98-99	23.307871370297423	26.51816660945262	26.350430103769344	23.82353191648061
100-101	23.08862243599543	26.6387594535978	26.492233368562445	23.78038474184433
102-103	23.000797770530184	26.507111581710397	26.635066162973374	23.857024484786045
104-105	22.937388155901807	26.719372429338613	26.39102965363589	23.952209761123694
106-107	22.935190391597303	26.786255274337506	26.382899611329435	23.895654722735753
108-109	22.999913657097682	26.715492501089567	26.400649275130778	23.88394456668198
110-111	22.91149220991701	26.91054294660203	26.388521550289145	23.789443293191813
112-113	22.92462029259506	26.974646626649474	26.302838248705356	23.79789483205011
114-115	22.820636397491157	27.091945880603223	26.325568677758866	23.761849044146757
116-117	23.010254365109198	26.968469251588168	26.25981444761869	23.761461935683943
118-119	22.932866990140866	26.911423425963243	26.153968138599076	24.001741445296812
120-121	22.923936644388014	27.025646756062216	26.26149057223538	23.78892602731439
122-123	22.917516493617672	27.051926445733688	26.21850133843156	23.81205572221708
124-125	22.90669819556043	27.12731152225734	26.052927641717687	23.913062640464542
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	776.0
1	585.5
2	344.0
3	266.5
4	225.5
5	229.0
6	243.5
7	260.5
8	320.5
9	374.5
10	389.0
11	440.5
12	534.5
13	612.5
14	704.0
15	827.0
16	969.0
17	1120.0
18	1285.0
19	1559.5
20	1950.0
21	2458.0
22	3108.5
23	3951.0
24	5231.5
25	7083.5
26	9848.0
27	13543.5
28	17817.0
29	22953.5
30	28923.0
31	36716.5
32	44971.5
33	51346.5
34	56726.5
35	63039.5
36	70876.0
37	77922.0
38	84558.0
39	90999.0
40	98581.0
41	106711.5
42	112778.0
43	119081.0
44	124058.5
45	128490.5
46	133176.0
47	135917.5
48	138402.5
49	140675.0
50	141770.5
51	138729.0
52	131137.5
53	125103.5
54	120145.0
55	112879.0
56	104125.0
57	95374.5
58	87800.5
59	78729.5
60	66549.5
61	54955.0
62	45617.5
63	35913.5
64	27405.0
65	21502.0
66	16430.5
67	12405.5
68	9491.5
69	7389.0
70	6013.0
71	4864.5
72	3939.0
73	3397.5
74	2760.0
75	2133.5
76	1633.0
77	1136.0
78	739.5
79	495.0
80	340.5
81	239.0
82	165.5
83	103.0
84	73.5
85	60.5
86	51.0
87	35.0
88	22.0
89	19.5
90	21.0
91	18.0
92	9.0
93	6.5
94	6.5
95	5.5
96	6.0
97	7.5
98	8.5
99	8.5
100	15.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.036090057761711654
2	0.018470826958597742
3	0.02387405773821934
4	0.02193594234987681
5	0.014829519259287539
6	0.005344500010277885
7	0.005432596164293454
8	0.006607211551167714
9	0.003377019237263498
10-11	0.005329817317941956
12-13	0.007972701938409042
14-15	0.002730980774482655
16-17	0.002202403850389238
18-19	4.404807700778477E-5
20-21	0.0038468653920132024
22-23	0.0010718365405227627
24-25	0.00185001923432696
26-27	0.0012480288485539016
28-29	0.0024079615430922336
30-31	0.0014535865412568974
32-33	1.4682692335928257E-5
34-35	0.006166730781089867
36-37	0.007502855783659338
38-39	0.0012480288485539016
40-41	0.0010571538481868345
42-43	0.0022170865427251665
44-45	9.2500961716348E-4
46-47	0.01924900965240194
48-49	0.002760346159154512
50-51	0.009587798095361151
52-53	0.0016444615416239643
54-55	2.349230773748521E-4
56-57	1.7619230803113907E-4
58-59	0.0064603846278084315
60-61	8.515961554838387E-4
62-63	0.009543750018353366
64-65	0.009323509633314441
66-67	5.8730769343713026E-5
68-69	0.003773451930333561
70-71	0.004771875009176683
72-73	0.010997336559610263
74-75	0.0014535865412568974
76-77	8.662788478197669E-4
78-79	2.7897115438263687E-4
80-81	0.003993692315372485
82-83	0.0011305673098664757
84-85	9.2500961716348E-4
86-87	0.01270052887057794
88-89	0.0
90-91	3.670673083982064E-4
92-93	0.008530644247174316
94-95	0.006475067320144361
96-97	0.004492903854794046
98-99	8.515961554838387E-4
100-101	0.01513785579834203
102-103	0.007502855783659338
104-105	0.007033009628909634
106-107	0.01640056733923186
108-109	0.010042961557774926
110-111	0.01833868272757439
112-113	0.0024520096201000184
114-115	0.013331884641022855
116-117	0.00546196154896531
118-119	0.004478221162458117
120-121	0.007091740398253347
122-123	0.007781826938041975
124-125	0.05670455780135492
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	3405370.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.36028271303489
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.67323549941102	41.33931428344428
2	12.414447230494515	13.745346168124586
3	5.124621696456523	8.511015177395567
4	2.594973569478266	5.746338817566802
5	1.4612167385963457	4.044668587685625
6	0.9461417480480534	3.142720479512715
7	0.6252508596938299	2.422984505145297
8	0.4396506110140471	1.947134569655694
9	0.3130760836910471	1.5598782453443527
>10	1.3244320657148105	12.253083239790495
>50	0.05859107589023558	2.2015627773833115
>100	0.02259866865816239	2.208397712313787
>500	0.0013899386140640757	0.5365305055216527
>1k	3.742142391243999E-4	0.3410249311159116
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	1.4682692335928255E-5	2.936538467185651E-5
12-13	0.0	0.0	0.0	2.936538467185651E-5	2.0555769270299556E-4
14-15	0.0	0.0	0.0	2.936538467185651E-5	2.642884620467086E-4
16-17	0.0	0.0	0.0	4.404807700778476E-5	3.3770192372634984E-4
18-19	0.0	0.0	0.0	5.873076934371302E-5	3.964326930700629E-4
20-21	0.0	0.0	0.0	1.027788463514978E-4	5.285769240934171E-4
22-23	1.4682692335928255E-5	0.0	0.0	2.6428846204670857E-4	5.579423087652736E-4
24-25	2.936538467185651E-5	0.0	0.0	7.781826938041975E-4	5.579423087652736E-4
26-27	2.936538467185651E-5	0.0	0.0	0.0014095384642491124	6.166730781089867E-4
28-29	2.936538467185651E-5	0.0	0.0	0.003846865392013203	6.460384627808432E-4
30-31	2.936538467185651E-5	0.0	0.0	0.011731471176406674	6.460384627808432E-4
32-33	2.936538467185651E-5	0.0	0.0	0.029336019287184656	6.460384627808432E-4
34-35	2.936538467185651E-5	0.0	0.0	0.052945788563357285	7.341346167964128E-4
36-37	2.936538467185651E-5	0.0	0.0	0.09279461556306656	7.341346167964128E-4
38-39	2.936538467185651E-5	0.0	0.0	0.1584996637663455	7.635000014682693E-4
40-41	2.936538467185651E-5	0.0	0.0	0.2315754235222604	7.635000014682693E-4
42-43	2.936538467185651E-5	0.0	0.0	0.2985431832664292	7.928653861401258E-4
44-45	2.936538467185651E-5	0.0	0.0	0.3750693757212873	8.956442324916235E-4
46-47	5.873076934371302E-5	0.0	0.0	0.4676584335916508	9.690576941712648E-4
48-49	5.873076934371302E-5	0.0	0.0	0.5631693472368642	9.690576941712648E-4
50-51	5.873076934371302E-5	0.0	0.0	0.660207260885014	0.0010277884635149779
52-53	5.873076934371302E-5	0.0	0.0	0.7587574918437644	0.0011158846175305472
54-55	5.873076934371302E-5	0.0	0.0	0.8692300689792887	0.0011452500022024038
56-57	5.873076934371302E-5	0.0	0.0	0.9921829346003519	0.0011746153868742604
58-59	5.873076934371302E-5	0.0	0.0	1.1325054252548181	0.0012186634638820452
60-61	5.873076934371302E-5	0.0	0.0	1.2804335505392952	0.0012333461562179734
62-63	5.873076934371302E-5	0.0	0.0	1.4289783489018815	0.001321442310233543
64-65	5.873076934371302E-5	0.0	0.0	1.5867145126667586	0.001321442310233543
66-67	5.873076934371302E-5	0.0	0.0	1.7704537245585648	0.0013361250025694712
68-69	5.873076934371302E-5	0.0	0.0	1.9811650422714713	0.001380173079577256
70-71	5.873076934371302E-5	0.0	0.0	2.2038721196228312	0.001438903848920969
72-73	5.873076934371302E-5	0.0	0.0	2.429559783518384	0.0016444615416239645
74-75	5.873076934371302E-5	1.4682692335928255E-5	0.0	2.662838399351612	0.0017619230803113904
76-77	5.873076934371302E-5	2.936538467185651E-5	0.0	2.920695842155184	0.0017619230803113904
78-79	7.341346167964128E-5	2.936538467185651E-5	0.0	3.20890241001712	0.0018500192343269602
80-81	8.809615401556952E-5	2.936538467185651E-5	0.0	3.513054381755874	0.0018793846189988166
82-83	8.809615401556952E-5	2.936538467185651E-5	0.0	3.8350164592981084	0.0019968461576862426
84-85	8.809615401556952E-5	2.936538467185651E-5	0.0	4.1623524022352925	0.0020408942346940274
86-87	8.809615401556952E-5	2.936538467185651E-5	0.0	4.518437056766225	0.002143673081045525
88-89	8.809615401556952E-5	2.936538467185651E-5	0.0	4.910097874827111	0.00218772115805331
90-91	8.809615401556952E-5	2.936538467185651E-5	0.0	5.324414087162335	0.002202403850389238
92-93	8.809615401556952E-5	2.936538467185651E-5	0.0	5.757553511072218	0.0022611346197329513
94-95	8.809615401556952E-5	2.936538467185651E-5	0.0	6.195406079221935	0.002319865389076664
96-97	8.809615401556952E-5	2.936538467185651E-5	0.0	6.655825945491973	0.002584153851123373
98-99	8.809615401556952E-5	2.936538467185651E-5	0.0	7.15584503299201	0.002760346159154512
100-101	8.809615401556952E-5	5.873076934371302E-5	0.0	7.69490539941328	0.002760346159154512
102-103	8.809615401556952E-5	5.873076934371302E-5	0.0	8.250057996634727	0.0028190769284982247
104-105	1.1746153868742604E-4	5.873076934371302E-5	0.0	8.798104170765585	0.002877807697841938
106-107	1.1746153868742604E-4	5.873076934371302E-5	0.0	9.361170739156098	0.002877807697841938
108-109	1.1746153868742604E-4	5.873076934371302E-5	0.0	9.948860182593961	0.002877807697841938
110-111	1.1746153868742604E-4	5.873076934371302E-5	0.0	10.57915879919069	0.0029071730825137943
112-113	1.1746153868742604E-4	5.873076934371302E-5	0.0	11.232362415831467	0.0029071730825137943
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	14680	0.0	30.001505	1
TACATGG	14820	0.0	28.908527	2
GTATCAA	8905	0.0	28.1375	1
CATGGGG	8705	0.0	27.476454	4
ACATGGG	15640	0.0	27.050055	3
TCAACGC	9905	0.0	24.50807	4
AACGCAG	10235	0.0	24.182573	6
CAACGCA	10105	0.0	24.140404	5
ATCAACG	10145	0.0	23.754084	3
TATCAAC	10805	0.0	22.909216	2
ACGCAGA	11580	0.0	20.911388	7
ATGGGGA	4675	0.0	20.362701	5
CGCAGAG	11825	0.0	20.17624	8
GGTATCA	6765	0.0	18.915073	1
GTATAGC	1540	0.0	17.391186	1
GTATATA	1850	0.0	16.728962	1
GCAGAGT	13355	0.0	16.617357	9
ATGGGGG	4625	0.0	16.46627	5
ACGGCGT	330	7.517254E-6	16.226528	8
TACGGCG	295	5.2363E-5	16.134851	7
>>END_MODULE