FastQCFastQC Report
Mon 15 Aug 2016
SRR2920958_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920958_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3405370
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35710.10486378866319959No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA105350.032.6366461
GGTATCA77750.029.5836091
TCAACGC131600.026.1623364
ATCAACG132800.025.9247823
CAACGCA133450.025.6812955
AACGCAG136100.025.2638656
CATGGGG83050.024.673084
TATCAAC139800.024.6653062
GTACATG149600.024.1382241
TACATGG148550.024.0944482
ACATGGG151350.022.7079933
ACGCAGA153350.022.3069217
CGCAGAG156900.021.6897328
AGTACTT78100.020.8761712-13
TATACCG3251.4571924E-820.1382755
GAGTACT74400.019.71493312-13
GCAGAGT175550.018.9454469
AGAGTAC156250.018.9442110-11
ATGGGGA46200.017.9013525
GTACTTT82300.017.75018714-15
Produced by FastQC (version 0.11.5)