##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920958_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3405370
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.007556007129914	31.0	30.0	34.0	26.0	34.0
2	30.11604700810778	31.0	30.0	34.0	26.0	34.0
3	30.224504532547122	31.0	30.0	34.0	26.0	34.0
4	33.78503510631737	35.0	35.0	37.0	30.0	37.0
5	33.29260462152424	35.0	33.0	37.0	27.0	37.0
6	33.05611078972329	35.0	33.0	37.0	26.0	37.0
7	32.909045419440474	35.0	33.0	37.0	26.0	37.0
8	32.8444674147009	35.0	33.0	37.0	26.0	37.0
9	34.18050432111635	38.0	34.0	39.0	25.0	39.0
10-11	34.07025521455819	37.5	33.5	39.0	25.0	39.0
12-13	33.96591530435753	37.5	33.0	39.0	25.0	39.0
14-15	34.865715913395604	38.5	33.5	41.0	25.0	41.0
16-17	34.86259775002423	38.5	33.5	41.0	25.0	41.0
18-19	34.90491473760561	38.5	33.5	41.0	25.0	41.0
20-21	34.87543497476045	39.0	34.0	41.0	24.5	41.0
22-23	34.84055036603952	39.0	34.0	41.0	24.0	41.0
24-25	34.80961452059542	39.0	34.0	41.0	24.0	41.0
26-27	34.65630254568519	39.0	34.0	41.0	23.5	41.0
28-29	34.54144380786816	39.0	33.5	41.0	23.0	41.0
30-31	34.376656574762805	38.0	33.0	41.0	22.5	41.0
32-33	34.23496697862494	38.0	33.0	40.0	22.0	41.0
34-35	34.108804182805386	38.0	33.0	40.0	20.5	41.0
36-37	33.96166202204166	38.0	33.0	40.0	19.0	41.0
38-39	33.81906209897897	38.0	33.0	40.0	18.0	41.0
40-41	33.63332765602563	38.0	33.0	40.0	17.0	41.0
42-43	33.482985696121126	38.0	32.5	40.0	15.5	41.0
44-45	33.26221614685041	38.0	32.0	40.0	14.5	41.0
46-47	33.11034806790451	38.0	32.0	40.0	11.0	41.0
48-49	32.89896883451725	38.0	32.0	40.0	9.0	41.0
50-51	32.70313592942911	38.0	31.5	40.0	8.0	41.0
52-53	32.467444947245085	37.0	31.0	40.0	7.0	41.0
54-55	32.282533028716415	37.0	31.0	40.0	6.0	41.0
56-57	32.1541390509695	37.0	31.0	40.0	2.0	41.0
58-59	32.093785403641895	37.0	31.0	40.0	2.0	41.0
60-61	31.83572372458793	36.0	31.0	40.0	2.0	41.0
62-63	31.5876638955532	36.0	30.5	39.5	2.0	41.0
64-65	31.328737699574496	35.5	30.0	39.0	2.0	41.0
66-67	31.028972475824947	35.0	30.0	39.0	2.0	41.0
68-69	30.718732913016794	35.0	29.5	38.5	2.0	40.5
70-71	30.366845746570856	35.0	29.0	38.0	2.0	40.0
72-73	30.091559360656845	35.0	29.0	37.0	2.0	39.5
74-75	29.827918552169074	35.0	29.0	37.0	2.0	39.0
76-77	29.495100532394424	34.0	29.0	36.0	2.0	39.0
78-79	29.166261815896657	34.0	29.0	36.0	2.0	38.5
80-81	28.89547229816437	34.0	29.0	35.0	2.0	37.0
82-83	28.630594766501144	34.0	29.0	35.0	2.0	37.0
84-85	28.420284139462083	34.0	28.0	35.0	2.0	36.0
86-87	28.180881959963237	34.0	28.0	35.0	2.0	36.0
88-89	28.03991974440369	34.0	27.0	35.0	2.0	36.0
90-91	27.84459368585499	34.0	27.0	35.0	2.0	35.0
92-93	27.974925485336396	34.0	28.0	35.0	2.0	35.0
94-95	27.916889794647865	34.0	27.5	35.0	2.0	35.0
96-97	27.849903681538276	34.0	27.0	35.0	2.0	35.0
98-99	27.794798509413077	34.0	27.0	35.0	2.0	35.0
100-101	27.658262244631274	34.0	27.0	35.0	2.0	35.0
102-103	26.36122374367543	31.5	24.5	34.0	2.0	35.0
104-105	27.41585422435741	33.5	27.0	35.0	2.0	35.0
106-107	27.608895655978646	34.0	27.0	35.0	2.0	35.0
108-109	27.588549408727978	34.0	27.0	35.0	2.0	35.0
110-111	27.52584755841509	34.0	27.0	35.0	2.0	35.0
112-113	27.423663214276274	34.0	27.0	35.0	2.0	35.0
114-115	27.32959311322999	34.0	27.0	35.0	2.0	35.0
116-117	27.20167103721475	34.0	27.0	35.0	2.0	35.0
118-119	27.072394923312295	34.0	27.0	35.0	2.0	35.0
120-121	26.93485215997087	34.0	26.5	35.0	2.0	35.0
122-123	26.701248175675477	34.0	25.0	35.0	2.0	35.0
124-125	25.59696582162878	32.5	22.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	108551.0
3	36395.0
4	17980.0
5	14018.0
6	15394.0
7	18252.0
8	20693.0
9	22733.0
10	23794.0
11	25126.0
12	25920.0
13	27763.0
14	29770.0
15	25050.0
16	19659.0
17	17665.0
18	16413.0
19	15763.0
20	15421.0
21	16378.0
22	18740.0
23	23949.0
24	32374.0
25	42438.0
26	52155.0
27	60665.0
28	71718.0
29	84409.0
30	99607.0
31	118742.0
32	143552.0
33	177399.0
34	228740.0
35	311050.0
36	492088.0
37	690422.0
38	244119.0
39	465.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.23440228645029	19.786091857576334	13.61763537604915	25.361870479924224
2	15.71959330092959	20.892279483450835	38.51204921171515	24.87607800390442
3	18.562700516111033	24.699620441814538	29.82173245919933	26.9159465828751
4	13.025160544638922	16.65206835830629	36.30474328882099	34.018027808233796
5	13.594655335266282	37.74885338884686	34.51337752172217	14.143113754164688
6	32.717098219188784	36.6144618010385	16.944576428169956	13.723863551602767
7	28.041030942064204	32.28981259736846	21.44342460946161	18.225731851105724
8	26.105556777597034	35.93249318879111	19.75369517443068	18.208254859181174
9	26.348677089694256	15.472259843146446	19.536964503960405	38.64209856319889
10-11	25.132542887915022	26.08082186429711	27.868972349487347	20.91766289830052
12-13	25.703006106022062	23.356536677324804	28.086130170656993	22.854327045996133
14-15	22.8548605595477	24.864618923859968	25.483101010908033	26.7974195056843
16-17	22.91440993991348	27.83339984480819	26.28933975035651	22.962850464921818
18-19	22.95622227878809	26.854542375365902	28.043140400479484	22.146094945366528
20-21	23.755886029549984	26.041098819504494	27.681021842926423	22.5219933080191
22-23	23.228314714262403	25.873956215652356	27.533921935746985	23.363807134338252
24-25	22.934951351229103	26.176876131078604	27.758913923697804	23.129258593994496
26-27	22.916900105985736	26.34615613371326	27.630350190552573	23.106593569748437
28-29	22.901340466982642	26.43143657798655	27.75093260540034	22.916290349630465
30-31	23.13335972925771	26.28782934447445	27.559592030232338	23.0192188960355
32-33	22.840640268062906	26.35188719645982	27.460880568317798	23.346591967159476
34-35	23.058152329400624	26.364278039429713	27.624016063737976	22.953553567431687
36-37	23.280295914867928	26.136414874172292	27.51705718333048	23.066232027629297
38-39	23.18572828039379	26.27844334683075	27.408833685088656	23.1269946876868
40-41	23.133474077770945	26.320654094449957	27.500757135358178	23.04511469242092
42-43	23.306843271647214	26.145116620355935	27.341039435182868	23.207000672813983
44-45	23.17565283187793	26.301103530480958	27.23666203839284	23.286581599248272
46-47	23.227203619848357	26.350124797286114	27.086085818642434	23.336585764223095
48-49	23.320901687679246	26.528283890945982	26.87509023383175	23.275724187543023
50-51	23.153631965881754	26.679374851305425	26.87261170817387	23.294381474638946
52-53	23.423977553813305	26.494158016854026	26.779464512048932	23.302399917283733
54-55	23.202867275701813	26.6291489423888	26.695358419009345	23.472625362900047
56-57	23.183563172812853	26.57562673481077	26.66049845060287	23.58031164177351
58-59	23.109332002526692	26.43976570087722	26.910561069822847	23.54034122677324
60-61	23.209532435126366	26.50474832706187	26.946252997540277	23.339466240271488
62-63	23.027448908887475	26.63210036205161	26.959910793726944	23.38053993533397
64-65	22.982676932188333	26.50072737613454	26.877179778238407	23.639415913438715
66-67	23.006101087878992	26.67873919663281	26.77122932705315	23.543930388435047
68-69	23.268938785329347	26.414488303324873	26.730738106155243	23.585834805190533
70-71	22.99425183360868	26.35034028393028	26.87662886256336	23.77877901989768
72-73	23.183765422868838	26.443538809036234	26.69555717385742	23.67713859423751
74-75	23.161379035665462	26.490055429476005	26.645257374846036	23.7033081600125
76-77	23.04033783946516	26.348990540706446	26.785637710760497	23.82503390906789
78-79	23.150804036558732	26.458626154209934	26.663097740120463	23.727472069110867
80-81	23.0820553007682	26.491504683981766	26.60427917435973	23.822160840890305
82-83	22.97303390755343	26.552322033263493	26.640492981795187	23.834151077387887
84-85	23.0774935570483	26.608652565821345	26.439532885451083	23.87432099167927
86-87	23.0276846974467	26.381734371133124	26.5012443757082	24.089336555711974
88-89	22.92228512579131	26.399578301627137	26.544599537823753	24.133537034757804
90-91	22.888853704504434	26.47375897691382	26.55208666246804	24.085300656113702
92-93	22.85416910519292	26.471975220216358	26.61271337074112	24.061142303849596
94-95	22.664924549967463	26.38518176722241	26.75815471717454	24.191738965635587
96-97	22.894256831125105	26.200235167894515	26.665768914869194	24.239739086111186
98-99	22.832789090569975	26.297653673620296	26.497135107072754	24.37242212873697
100-101	22.64808045110706	26.399003466484483	26.619983774902323	24.332932307506134
102-103	22.642018747797955	26.175306171393707	26.68753900161448	24.49513607919386
104-105	22.544754903587766	26.392109614083775	26.42945615621693	24.633679326111533
106-107	22.520877179276773	26.40936011893399	26.433428274546728	24.63633442724251
108-109	22.529803425154412	26.407919972039558	26.45450113222177	24.60777547058426
110-111	22.401869077901303	26.55649653336803	26.38858028530489	24.653054103425774
112-113	22.369428869145167	26.63821115289325	26.302510099959942	24.689849878001645
114-115	22.26185630230333	26.73121191882987	26.272299493259414	24.73463228560738
116-117	22.3153940112325	26.599388946827567	26.27807647768346	24.80714056425647
118-119	22.25164209278654	26.60083122228794	26.19684967222753	24.950677012697987
120-121	22.141445543138452	26.724973492781416	26.257982450099547	24.875598513980584
122-123	22.054924698494442	26.729479590889817	26.18185363515801	25.033742075457738
124-125	21.958710390952852	26.77015072902214	26.125164030715837	25.145974849309177
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	41.0
1	52.0
2	136.5
3	223.5
4	203.5
5	183.5
6	225.5
7	425.5
8	676.5
9	723.5
10	615.5
11	542.0
12	597.0
13	671.0
14	761.0
15	896.5
16	1030.0
17	1203.5
18	1449.5
19	1767.0
20	2088.0
21	2586.5
22	3286.5
23	4140.5
24	5365.5
25	7117.0
26	9847.5
27	13656.5
28	18009.5
29	23319.0
30	29714.5
31	37683.5
32	46295.0
33	52566.5
34	57486.0
35	63795.5
36	71337.0
37	78143.5
38	85131.0
39	91789.5
40	99068.5
41	106826.5
42	112794.5
43	119758.5
44	125547.5
45	129355.0
46	133173.0
47	136304.5
48	139309.5
49	141452.0
50	144155.5
51	141240.0
52	132064.0
53	125956.0
54	121085.5
55	113705.0
56	104184.0
57	94844.0
58	87346.5
59	78219.0
60	65302.5
61	53316.0
62	43893.5
63	33678.5
64	24718.0
65	19126.0
66	14491.5
67	10683.5
68	8112.5
69	6302.5
70	5116.0
71	4114.0
72	3324.5
73	3046.5
74	2432.5
75	1782.0
76	1319.5
77	878.0
78	561.0
79	348.5
80	228.0
81	144.0
82	98.0
83	50.5
84	30.5
85	27.0
86	20.5
87	15.0
88	8.5
89	5.5
90	5.5
91	5.5
92	3.5
93	4.5
94	4.0
95	4.5
96	8.5
97	6.0
98	2.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04892273086331294
2	5.8730769343713026E-5
3	0.0027897115438263683
4	0.12600686562693628
5	0.01626842310820851
6	0.0019968461576862426
7	0.003964326930700628
8	0.009426288479665939
9	0.02328675004478221
10-11	0.018294634650566605
12-13	0.01810375965019954
14-15	0.026605038512701993
16-17	0.035106317375204456
18-19	0.03745554814895298
20-21	0.02049703850095584
22-23	0.02206808658090017
24-25	0.02086410580935405
26-27	0.019572028883792364
28-29	0.019410519268097153
30-31	0.023609769276172633
32-33	0.02337484619879778
34-35	0.027368538514170267
36-37	0.029732451980254714
38-39	0.029805865441934355
40-41	0.032037634676995455
42-43	0.029703086595582857
44-45	0.02739790389884212
46-47	0.03669204814748471
48-49	0.02996737505762957
50-51	0.021730384657173817
52-53	0.028220134669654104
54-55	0.03005547121164514
56-57	0.024079615430922338
58-59	0.026972105821100205
60-61	0.0390265962288973
62-63	0.04620643278116622
64-65	0.03976073084569372
66-67	0.03591386545368051
68-69	0.027632826976216975
70-71	0.03388765391132241
72-73	0.03679482699383621
74-75	0.022714125043681012
76-77	0.019851000038175
78-79	0.02892490390177866
80-81	0.02313992312142293
82-83	0.0348713942978296
84-85	0.019983144269198356
86-87	0.030907067367128976
88-89	0.030569365443402625
90-91	0.03177334621494874
92-93	0.024094298123258265
94-95	0.02054108657796363
96-97	0.016694221185950424
98-99	0.02327206735244628
100-101	0.0225526154279858
102-103	0.025357009664148094
104-105	0.022640711582001368
106-107	0.013390615410366569
108-109	0.016444615416239646
110-111	0.020452990423948058
112-113	0.011922346176773744
114-115	0.01386046156511627
116-117	0.012597750024226443
118-119	0.007752461553370118
120-121	0.0048599711631922525
122-123	0.00370003846865392
124-125	0.060022846269274704
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	3405370.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.694739976720385
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.5809078645643	34.57805123673661
2	14.581300661398448	14.492278897811536
3	6.203970319446393	9.249140755445382
4	3.111220324971625	6.1844514023900965
5	1.829019836293248	4.544633258842831
6	1.1511585410908212	3.4323914622893485
7	0.7824821286401211	2.721967214344104
8	0.5481465670788765	2.179200089609335
9	0.38196439422419814	1.7083459126203342
>10	1.7163095502278107	14.239931122058422
>50	0.08007967567663689	2.727156419690334
>100	0.03118135227488528	2.7827357053231006
>500	0.0017238089300268635	0.6034897759738468
>1k	5.349751824357032E-4	0.5562267468646684
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3571	0.10486378866319959	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.873076934371302E-5
2	0.0	0.0	0.0	0.0	5.873076934371302E-5
3	0.0	0.0	0.0	0.0	5.873076934371302E-5
4	0.0	0.0	0.0	2.936538467185651E-5	5.873076934371302E-5
5	0.0	0.0	0.0	2.936538467185651E-5	8.809615401556952E-5
6	0.0	0.0	0.0	2.936538467185651E-5	8.809615401556952E-5
7	0.0	0.0	0.0	2.936538467185651E-5	8.809615401556952E-5
8	0.0	0.0	0.0	2.936538467185651E-5	8.809615401556952E-5
9	0.0	0.0	0.0	2.936538467185651E-5	8.809615401556952E-5
10-11	0.0	0.0	0.0	4.404807700778476E-5	8.809615401556952E-5
12-13	0.0	0.0	0.0	5.873076934371302E-5	3.083365390544933E-4
14-15	0.0	0.0	0.0	5.873076934371302E-5	4.111153854059911E-4
16-17	0.0	0.0	0.0	7.341346167964128E-5	4.6984615474970415E-4
18-19	0.0	0.0	0.0	8.809615401556952E-5	5.579423087652736E-4
20-21	0.0	0.0	0.0	1.3214423102335428E-4	6.460384627808432E-4
22-23	0.0	0.0	0.0	2.936538467185651E-4	7.341346167964128E-4
24-25	0.0	0.0	0.0	8.369134631479105E-4	7.635000014682693E-4
26-27	0.0	0.0	0.0	0.0015123173106006103	8.369134631479105E-4
28-29	0.0	0.0	0.0	0.004096471161723983	8.809615401556952E-4
30-31	0.0	0.0	0.0	0.011878298099765958	8.809615401556952E-4
32-33	0.0	0.0	0.0	0.029380067364192436	9.250096171634801E-4
34-35	0.0	0.0	0.0	0.05251999048561537	0.0010277884635149779
36-37	0.0	0.0	0.0	0.09233945210065278	0.0010277884635149779
38-39	0.0	0.0	0.0	0.15826474068897067	0.0010277884635149779
40-41	0.0	0.0	0.0	0.2308266062131281	0.0010277884635149779
42-43	0.0	0.0	0.0	0.2979411928806561	0.001042471155850906
44-45	0.0	0.0	0.0	0.3749519141825999	0.0010865192328586909
46-47	0.0	0.0	0.0	0.46792272205369756	0.0011305673098664757
48-49	0.0	0.0	0.0	0.5641237222386994	0.0011452500022024038
50-51	0.0	0.0	0.0	0.6616902128109428	0.0011452500022024038
52-53	0.0	0.0	0.0	0.7605194149240757	0.0012920769255616864
54-55	0.0	0.0	0.0	0.8714618382143496	0.0012920769255616864
56-57	2.936538467185651E-5	0.0	0.0	0.9954571749912638	0.0013067596178976148
58-59	2.936538467185651E-5	0.0	0.0	1.137879290649768	0.001321442310233543
60-61	2.936538467185651E-5	0.0	0.0	1.288244742862009	0.0013508076949053994
62-63	2.936538467185651E-5	0.0	0.0	1.438066935457821	0.001438903848920969
64-65	2.936538467185651E-5	0.0	0.0	1.5976384357646893	0.0014535865412568971
66-67	2.936538467185651E-5	0.0	0.0	1.7842995034313454	0.0015123173106006103
68-69	2.936538467185651E-5	0.0	0.0	1.998182282688812	0.001556365387608395
70-71	5.873076934371302E-5	0.0	0.0	2.2240167735077248	0.001556365387608395
72-73	5.873076934371302E-5	0.0	0.0	2.4529786777941895	0.0016297788492880363
74-75	5.873076934371302E-5	0.0	0.0	2.6893993897873063	0.0017325576956395338
76-77	5.873076934371302E-5	0.0	0.0	2.9505310729817906	0.0017619230803113904
78-79	5.873076934371302E-5	0.0	0.0	3.2441849197003556	0.001791288464983247
80-81	5.873076934371302E-5	0.0	0.0	3.5536079779877077	0.0018059711573191754
82-83	5.873076934371302E-5	0.0	0.0	3.880547488231822	0.001908750003670673
84-85	7.341346167964128E-5	0.0	0.0	4.212009267715402	0.001908750003670673
86-87	8.809615401556952E-5	0.0	0.0	4.570619345328114	0.002011528850022171
88-89	8.809615401556952E-5	0.0	0.0	4.966758384551459	0.0021143076963736685
90-91	8.809615401556952E-5	0.0	0.0	5.3882984815159585	0.0021143076963736685
92-93	8.809615401556952E-5	0.0	0.0	5.828749886209135	0.0021730384657173817
94-95	8.809615401556952E-5	0.0	0.0	6.2748541274516425	0.0021730384657173817
96-97	8.809615401556952E-5	0.0	0.0	6.744524089893316	0.0025694711587874444
98-99	8.809615401556952E-5	0.0	0.0	7.2562599658774225	0.0028190769284982247
100-101	8.809615401556952E-5	0.0	0.0	7.808769678478403	0.0028190769284982247
102-103	8.809615401556952E-5	0.0	0.0	8.376696218032109	0.0028924903901778663
104-105	8.809615401556952E-5	0.0	0.0	8.938720315266771	0.0029659038518575075
106-107	8.809615401556952E-5	0.0	0.0	9.51535369137568	0.0029659038518575075
108-109	8.809615401556952E-5	0.0	0.0	10.11878298099766	0.0029659038518575075
110-111	8.809615401556952E-5	0.0	0.0	10.766877020705532	0.0029659038518575075
112-113	1.1746153868742604E-4	0.0	0.0	11.439784810461123	0.0029659038518575075
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	10535	0.0	32.636646	1
GGTATCA	7775	0.0	29.583609	1
TCAACGC	13160	0.0	26.162336	4
ATCAACG	13280	0.0	25.924782	3
CAACGCA	13345	0.0	25.681295	5
AACGCAG	13610	0.0	25.263865	6
CATGGGG	8305	0.0	24.67308	4
TATCAAC	13980	0.0	24.665306	2
GTACATG	14960	0.0	24.138224	1
TACATGG	14855	0.0	24.094448	2
ACATGGG	15135	0.0	22.707993	3
ACGCAGA	15335	0.0	22.306921	7
CGCAGAG	15690	0.0	21.689732	8
AGTACTT	7810	0.0	20.87617	12-13
TATACCG	325	1.4571924E-8	20.138275	5
GAGTACT	7440	0.0	19.714933	12-13
GCAGAGT	17555	0.0	18.945446	9
AGAGTAC	15625	0.0	18.94421	10-11
ATGGGGA	4620	0.0	17.901352	5
GTACTTT	8230	0.0	17.750187	14-15
>>END_MODULE