FastQCFastQC Report
Mon 15 Aug 2016
SRR2920957_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920957_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2625013
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT29580.11268515622589298No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA29470.11226611068211852No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG71100.026.9467964
GTACATG134800.025.5196481
TACATGG135850.024.7476792
GTATCAA90450.024.0171241
ACATGGG135250.023.6251413
TCAACGC101700.021.003634
AACGCAG103800.020.4624966
CAACGCA105300.020.2843975
TATCAAC106850.020.15952
ATGGGGA38550.019.7552095
ATCAACG108550.019.350063
GTATTAG8500.018.9052121
ACGCAGA114400.018.3584697
CGCAGAG118500.017.6730738
GGTATCA73450.016.7731741
GAGTACT34700.016.45951712-13
ATGGGGG41050.015.5083875
GTATTAT11150.015.4796051
GCAGAGT128200.015.40779
GTATAAT13150.015.3882721
Produced by FastQC (version 0.11.5)