##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920957_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2625013
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.881181540815227	31.0	27.0	31.0	10.0	34.0
2	26.981114379243074	31.0	27.0	33.0	10.0	34.0
3	27.05145498327056	31.0	27.0	33.0	10.0	34.0
4	30.331774356926996	35.0	30.0	37.0	10.0	37.0
5	30.388499790286755	35.0	30.0	37.0	10.0	37.0
6	30.315926054461446	35.0	30.0	37.0	8.0	37.0
7	30.163463571418504	35.0	30.0	37.0	8.0	37.0
8	30.13037649718306	35.0	30.0	37.0	7.0	37.0
9	31.1511188706494	37.0	30.0	39.0	7.0	39.0
10-11	31.060691128005843	37.0	30.0	39.0	2.0	39.0
12-13	30.972532516981822	37.0	30.0	39.0	2.0	39.0
14-15	31.705600696072743	38.0	30.0	40.0	2.0	41.0
16-17	31.53966685117369	37.0	30.0	40.0	2.0	41.0
18-19	31.62713898940691	37.5	30.0	40.0	2.0	41.0
20-21	31.62239501290089	38.0	30.0	40.0	2.0	41.0
22-23	31.550661082440353	37.5	30.0	40.0	2.0	41.0
24-25	31.438009830808458	37.0	30.0	40.0	2.0	41.0
26-27	31.242559370182164	37.0	29.5	40.0	2.0	41.0
28-29	31.1252955699648	37.0	29.0	40.0	2.0	41.0
30-31	30.96817349095033	37.0	29.0	40.0	2.0	41.0
32-33	30.826273812739213	37.0	29.0	40.0	2.0	41.0
34-35	30.61671466007978	37.0	28.0	40.0	2.0	41.0
36-37	30.450967861873444	37.0	28.0	40.0	2.0	41.0
38-39	30.306933337092048	36.5	27.0	40.0	2.0	41.0
40-41	30.1947026548059	36.0	27.0	40.0	2.0	41.0
42-43	29.971577664567757	36.0	27.0	40.0	2.0	41.0
44-45	29.784369067886523	36.0	26.5	40.0	2.0	41.0
46-47	29.56058350949119	36.0	26.0	40.0	2.0	41.0
48-49	29.36042335790337	35.5	26.0	40.0	2.0	41.0
50-51	29.120829496844397	35.0	25.5	39.5	2.0	41.0
52-53	28.936244506217683	35.0	24.5	39.0	2.0	41.0
54-55	28.70326527906719	35.0	24.0	39.0	2.0	41.0
56-57	28.477663348714845	35.0	24.0	39.0	2.0	40.0
58-59	28.215322171737817	34.0	23.0	39.0	2.0	40.0
60-61	27.998782863170582	34.0	23.0	38.5	2.0	40.0
62-63	27.860282406220463	34.0	23.0	38.0	2.0	40.0
64-65	27.630216688450687	34.0	22.0	38.0	2.0	40.0
66-67	27.361699161108916	34.0	22.0	37.5	2.0	40.0
68-69	27.05924161137488	34.0	22.0	37.0	2.0	40.0
70-71	26.739714050939938	33.0	21.0	36.5	2.0	39.0
72-73	26.46834891865297	33.0	20.0	36.0	2.0	39.0
74-75	26.169786397248316	33.0	20.0	35.5	2.0	38.5
76-77	25.901045251966373	33.0	19.5	35.0	2.0	37.5
78-79	25.60065912054531	32.5	18.0	35.0	2.0	37.0
80-81	25.299351660353683	32.0	17.5	35.0	2.0	36.5
82-83	25.04246702778234	32.0	16.0	35.0	2.0	36.0
84-85	24.804136779513094	32.0	13.0	35.0	2.0	36.0
86-87	24.739378624029673	32.0	12.0	35.0	2.0	35.0
88-89	24.82834980245812	32.0	12.0	35.0	2.0	35.0
90-91	24.7339310319606	32.0	10.5	35.0	2.0	35.0
92-93	24.57607048041286	32.0	7.0	35.0	2.0	35.0
94-95	24.478274393307764	32.0	7.0	35.0	2.0	35.0
96-97	24.372609392791578	32.0	7.0	35.0	2.0	35.0
98-99	24.291576460764194	32.0	3.5	35.0	2.0	35.0
100-101	24.103754914737564	32.0	2.0	35.0	2.0	35.0
102-103	22.882752580653886	29.5	2.0	34.0	2.0	34.5
104-105	23.78830523886929	31.5	2.0	34.5	2.0	35.0
106-107	23.946413598713605	32.0	2.0	35.0	2.0	35.0
108-109	23.920519250761807	32.0	2.0	35.0	2.0	35.0
110-111	23.796005391211395	32.0	2.0	35.0	2.0	35.0
112-113	23.67794083305492	32.0	2.0	35.0	2.0	35.0
114-115	23.519026953390323	32.0	2.0	35.0	2.0	35.0
116-117	23.35942507713295	32.0	2.0	35.0	2.0	35.0
118-119	23.13289534185164	32.0	2.0	35.0	2.0	35.0
120-121	22.87498138104459	32.0	2.0	35.0	2.0	35.0
122-123	22.598624463955034	32.0	2.0	35.0	2.0	35.0
124-125	21.476816495765924	30.5	2.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	245078.0
3	32908.0
4	20777.0
5	19365.0
6	21660.0
7	22788.0
8	22863.0
9	24148.0
10	24652.0
11	24692.0
12	24855.0
13	24304.0
14	24347.0
15	21676.0
16	18438.0
17	16323.0
18	15517.0
19	15118.0
20	15700.0
21	16802.0
22	20032.0
23	26328.0
24	33977.0
25	42417.0
26	49325.0
27	55456.0
28	63684.0
29	72883.0
30	83910.0
31	97660.0
32	116317.0
33	140810.0
34	176913.0
35	231243.0
36	329006.0
37	351019.0
38	81968.0
39	54.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.393772755577	18.311889643692535	12.270157716107958	25.024179884622512
2	16.033765724094177	20.192375469365285	38.201866602147796	25.571992204392746
3	19.064689538075577	24.584440247948873	28.78286640969482	27.568003804280732
4	13.26249738994722	16.120448029848337	35.26063186901116	35.35642271119328
5	14.282100187377935	37.609700675368295	33.69606216771659	14.412136969537178
6	33.67376638734088	35.42149543676464	16.659275886398373	14.245462289496103
7	28.66107607040921	32.42752822600923	20.752802790523315	18.158592913058254
8	26.695287832055296	35.22129146500543	19.506953503114826	18.57646719982445
9	26.743041833003993	15.922705954466718	18.790774788118725	38.54347742441057
10-11	25.250025096323213	26.295746501801126	27.147478567168527	21.30674983470714
12-13	25.75912624000207	23.861110624300423	26.99728054849257	23.38248258720494
14-15	23.37745678910286	25.230980986081423	24.20928358322106	27.182278641594653
16-17	23.355796000530297	27.946096456005986	25.239966658387136	23.45814088507658
18-19	23.59503948839843	27.091690836402048	26.617883388320397	22.695386286879124
20-21	24.403309693031648	26.325481085726825	26.241592047837326	23.029617173404205
22-23	24.005318883483994	26.054814747653985	25.962795244319302	23.97707112454271
24-25	23.700054838795083	26.34536478846492	26.049208627995824	23.905371744744173
26-27	23.659906029563334	26.232235779814246	26.210254613901963	23.89760357672046
28-29	23.680115660777908	26.46319360513235	26.25362511393215	23.603065620157587
30-31	24.07317247852933	26.340564637543352	25.96157069626541	23.624692187661907
32-33	23.606530710514576	26.487240253667316	25.885300377560032	24.02092865825807
34-35	23.77300251217035	26.388532250948828	26.143525160944247	23.69494007593658
36-37	23.96999585868634	26.183312753760234	26.058082781316923	23.788608606236505
38-39	23.86235063661583	26.33292748804369	25.957552341470812	23.847169533869668
40-41	23.894096757271768	26.308838044922993	26.099769865000926	23.697295332804313
42-43	23.951256781346768	26.31862562927641	25.916863988674788	23.813253600702037
44-45	23.873324749140945	26.260695319583387	25.939759541025076	23.926220390250595
46-47	23.821899257592868	26.37448579428113	25.932085979616193	23.871528968509807
48-49	23.7591250330486	26.399546348424828	25.8275864959035	24.01374212262307
50-51	23.58527658971364	26.54321340425905	25.869388635274344	24.00212137075297
52-53	23.833886104082428	26.377641089876487	25.6656106497704	24.122862156270692
54-55	23.825537360062384	26.39061427979866	25.555360000670476	24.228488359468482
56-57	23.82545484190414	26.30270679432862	25.605717778496086	24.266120585271157
58-59	23.901979340250396	26.136156695293455	25.712126649735943	24.249737314720203
60-61	23.871733582172425	26.165323708913885	25.875970170547347	24.086972538366343
62-63	23.725959340424886	26.200013715748728	25.913240269438266	24.160786674388124
64-65	23.708998487657137	26.125813439106533	25.876588326709495	24.28859974652684
66-67	23.841756793307454	26.27675704092556	25.73077816846412	24.150707997302867
68-69	23.942477753893247	25.9390128566925	25.82030345946842	24.298205929945834
70-71	23.694766952909795	25.909108139404424	25.98080702390007	24.415317883785708
72-73	23.941853042325498	25.893470649631794	25.88931778536512	24.275358522677593
74-75	23.74975594842964	26.08173656144909	25.89716214452996	24.27134534559131
76-77	23.95220986698169	25.773978210094796	26.03518049872437	24.238631424199145
78-79	23.970910503894757	25.867670602218475	25.94761338322535	24.21380551066142
80-81	23.920681492583203	25.809887707482858	25.98970507475469	24.27972572517925
82-83	23.934032095943063	25.834489376749897	25.970566940961497	24.26091158634554
84-85	23.82562460940186	25.913278212923867	25.85282062095318	24.408276556721088
86-87	23.80715068545865	25.785487056363575	25.97882863086009	24.428533627317684
88-89	23.80287259529762	25.69497751058757	26.01663306048389	24.485516833630918
90-91	23.75857142857143	25.73013333333333	26.122780952380953	24.388514285714287
92-93	23.868654157538813	25.767387370225737	26.030460043813697	24.333498428421755
94-95	23.607057646974784	25.739324237445533	26.170478843726936	24.483139271852753
96-97	23.769604486304598	25.58641308022946	26.170211752738936	24.473770680727007
98-99	23.68548372042879	25.68712030619697	25.993599182011877	24.63379679136236
100-101	23.58322901459802	25.843638678129377	26.06820892653402	24.504923380738585
102-103	23.35650654955287	25.828735359584883	26.22158471272546	24.59317337813679
104-105	23.30967500933881	25.937495773485086	26.04718038356087	24.70564883361523
106-107	23.219955443399897	26.026842170036733	26.168296230139752	24.584906156423614
108-109	23.28074994666098	26.025957054466765	26.17073440214575	24.52255859672651
110-111	23.145148230775533	26.225825933384268	26.014277249943323	24.61474858589688
112-113	23.18553256658273	26.2880533957096	26.020560548281285	24.505853489426386
114-115	23.24464103747678	26.380072990211644	25.975363469879298	24.39992250243228
116-117	23.184707018109307	26.197102449751185	26.06168456872386	24.55650596341565
118-119	23.24173130163829	26.082901660997177	25.97316285117467	24.702204186189864
120-121	23.17726043022723	26.22450328577541	26.08645461995376	24.511781664043607
122-123	23.165263182357968	26.2645359967723	25.95591930285887	24.614281518010863
124-125	23.22229530016899	26.317856796404694	25.812545346814147	24.647302556612168
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1845.0
1	1227.5
2	513.0
3	360.0
4	282.0
5	262.0
6	264.5
7	320.0
8	415.5
9	448.5
10	447.0
11	487.5
12	542.0
13	590.0
14	667.0
15	762.0
16	889.0
17	1063.5
18	1254.0
19	1446.0
20	1740.5
21	2161.5
22	2710.5
23	3494.0
24	4548.0
25	5979.0
26	8246.0
27	11074.0
28	13452.5
29	16380.5
30	19900.0
31	24146.5
32	28905.5
33	32454.5
34	36342.0
35	40227.5
36	44278.0
37	49720.0
38	54415.0
39	57457.0
40	61070.0
41	65792.0
42	70679.5
43	76997.5
44	82526.0
45	87914.5
46	93560.5
47	97479.0
48	101770.5
49	106677.0
50	112973.0
51	115860.0
52	113503.5
53	111168.0
54	108193.0
55	102774.0
56	95977.0
57	89422.5
58	84018.5
59	76381.5
60	65420.5
61	53673.0
62	43014.5
63	33364.5
64	25111.0
65	19261.5
66	14507.5
67	10767.0
68	7947.0
69	6055.0
70	4843.0
71	3788.0
72	2877.5
73	2324.5
74	1831.5
75	1419.0
76	1055.5
77	718.0
78	460.5
79	294.0
80	199.0
81	131.5
82	84.0
83	66.0
84	49.5
85	32.0
86	26.0
87	23.0
88	18.5
89	12.0
90	7.5
91	8.0
92	9.0
93	8.5
94	7.5
95	9.0
96	11.5
97	9.5
98	9.5
99	9.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03546649102309208
2	0.01744753264079073
3	0.022895124709858578
4	0.020761801941552287
5	0.014133263340029172
6	0.0046095009815189485
7	0.004914261376991276
8	0.0056761623656720935
9	0.002895223756987108
10-11	0.004819023753406173
12-13	0.008342815826054957
14-15	0.002571415836797761
16-17	0.0024761782132126584
18-19	5.714257415106135E-5
20-21	0.004457120783782785
22-23	9.904712852850633E-4
24-25	0.001771419798682902
26-27	0.0014666594032105745
28-29	0.003638077220950906
30-31	0.0014095168290595133
32-33	0.0
34-35	0.007276154441901812
36-37	0.009219001963037897
38-39	0.001485706927927595
40-41	0.0012190415818893086
42-43	0.002799986133402006
44-45	0.0012571366313233498
46-47	0.0219808435234416
48-49	0.003466649498497722
50-51	0.011238039583042064
52-53	0.0020952277188722493
54-55	1.5238019773616357E-4
56-57	2.8571287075530676E-4
58-59	0.007580914837374138
60-61	8.952336616999612E-4
62-63	0.011161849484173984
64-65	0.010571376217946349
66-67	1.142851483021227E-4
68-69	0.004399978209631724
70-71	0.005580924742086992
72-73	0.012133273244742025
74-75	0.00211427524358927
76-77	0.0013714217796254723
78-79	3.9999801905742945E-4
80-81	0.004857118802840215
82-83	0.0015047544526446154
84-85	0.0012380891066063292
86-87	0.014609451457954685
88-89	0.0
90-91	4.952356426425316E-4
92-93	0.010018998001152756
94-95	0.0076380574115251995
96-97	0.005161879198312541
98-99	0.0013714217796254723
100-101	0.01693324947343118
102-103	0.008209483153035813
104-105	0.008171388103601773
106-107	0.018514194024943878
108-109	0.011161849484173984
110-111	0.02125703758419482
112-113	0.003161889103025395
114-115	0.015790397990409953
116-117	0.006666633650957156
118-119	0.005485687118501889
120-121	0.008152340578884752
122-123	0.008990431666433651
124-125	0.05647591078596563
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2625013.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.491044525362696
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.30389774597322	37.01190364974568
2	11.683382519889486	11.798123740371686
3	5.297179600770441	8.023803932440298
4	2.9188473802174553	5.895026121491911
5	1.7869715859395359	4.511303095561554
6	1.1734892015912501	3.555041731654568
7	0.8017098323373112	2.8335416788674963
8	0.5640451315527489	2.2783382281235123
9	0.42028268722065193	1.9098460686327319
>10	1.9403373151107504	16.216221912495087
>50	0.0797449531551588	2.7137768944316
>100	0.02904241598814508	2.51807967160707
>500	5.348151269460689E-4	0.2046084253587013
>1k	5.348151269452298E-4	0.5303848492182325
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	2958	0.11268515622589298	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	2947	0.11226611068211852	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.80950494340409E-5	0.0
2	0.0	0.0	0.0	3.80950494340409E-5	0.0
3	0.0	0.0	0.0	3.80950494340409E-5	0.0
4	0.0	0.0	0.0	7.61900988680818E-5	0.0
5	3.80950494340409E-5	0.0	0.0	7.61900988680818E-5	0.0
6	3.80950494340409E-5	0.0	0.0	7.61900988680818E-5	0.0
7	3.80950494340409E-5	0.0	0.0	7.61900988680818E-5	0.0
8	3.80950494340409E-5	0.0	0.0	7.61900988680818E-5	0.0
9	3.80950494340409E-5	0.0	0.0	7.61900988680818E-5	0.0
10-11	3.80950494340409E-5	0.0	0.0	7.61900988680818E-5	0.0
12-13	3.80950494340409E-5	0.0	0.0	9.523762358510225E-5	0.0
14-15	3.80950494340409E-5	0.0	0.0	1.3333267301914313E-4	0.0
16-17	3.80950494340409E-5	0.0	0.0	1.7142772245318404E-4	0.0
18-19	3.80950494340409E-5	0.0	0.0	2.2857029660424538E-4	0.0
20-21	3.80950494340409E-5	0.0	0.0	3.0476039547232714E-4	0.0
22-23	3.80950494340409E-5	0.0	0.0	9.142811864169815E-4	0.0
24-25	3.80950494340409E-5	0.0	0.0	0.00211427524358927	0.0
26-27	3.80950494340409E-5	0.0	0.0	0.0034476019737807013	0.0
28-29	3.80950494340409E-5	0.0	0.0	0.007447582164354996	0.0
30-31	3.80950494340409E-5	0.0	0.0	0.020323708873060817	0.0
32-33	3.80950494340409E-5	0.0	0.0	0.04742833654538092	0.0
34-35	3.80950494340409E-5	0.0	0.0	0.08445672459526866	0.0
36-37	3.80950494340409E-5	0.0	0.0	0.1450088056706767	0.0
38-39	3.80950494340409E-5	0.0	0.0	0.24323689063635112	0.0
40-41	3.80950494340409E-5	0.0	0.0	0.35169349637506553	0.0
42-43	3.80950494340409E-5	0.0	0.0	0.4478454011465848	0.0
44-45	3.80950494340409E-5	0.0	0.0	0.556073436588695	0.0
46-47	3.80950494340409E-5	0.0	0.0	0.689253729410102	0.0
48-49	3.80950494340409E-5	0.0	0.0	0.8256340063839684	0.0
50-51	3.80950494340409E-5	0.0	0.0	0.9669094972101091	0.0
52-53	3.80950494340409E-5	0.0	0.0	1.110375453378707	0.0
54-55	3.80950494340409E-5	0.0	0.0	1.2661080154650661	0.0
56-57	3.80950494340409E-5	0.0	0.0	1.4457071260218521	0.0
58-59	3.80950494340409E-5	0.0	0.0	1.6421823434779181	0.0
60-61	3.80950494340409E-5	0.0	0.0	1.8469813292353219	0.0
62-63	3.80950494340409E-5	0.0	0.0	2.0481803328212087	0.0
64-65	3.80950494340409E-5	0.0	0.0	2.2632268868763696	0.0
66-67	3.80950494340409E-5	0.0	0.0	2.5134732666085844	0.0
68-69	3.80950494340409E-5	0.0	0.0	2.801928980923142	0.0
70-71	3.80950494340409E-5	0.0	0.0	3.1064417585741477	0.0
72-73	3.80950494340409E-5	0.0	0.0	3.4093164490994905	0.0
74-75	3.80950494340409E-5	0.0	0.0	3.7105721000238856	0.0
76-77	3.80950494340409E-5	0.0	0.0	4.046399008309673	0.0
78-79	3.80950494340409E-5	0.0	0.0	4.425882843246871	0.0
80-81	3.80950494340409E-5	0.0	0.0	4.836680808818851	0.0
82-83	3.80950494340409E-5	3.80950494340409E-5	0.0	5.256164445661793	0.0
84-85	3.80950494340409E-5	3.80950494340409E-5	0.0	5.681438530018708	0.0
86-87	3.80950494340409E-5	3.80950494340409E-5	0.0	6.141760059854942	0.0
88-89	3.80950494340409E-5	3.80950494340409E-5	0.0	6.64328138565409	0.0
90-91	3.80950494340409E-5	3.80950494340409E-5	0.0	7.181469196533502	0.0
92-93	5.7142574151061346E-5	3.80950494340409E-5	0.0	7.741447375689187	0.0
94-95	1.1428514830212269E-4	3.80950494340409E-5	0.0	8.299273184551847	0.0
96-97	1.1428514830212269E-4	3.80950494340409E-5	0.0	8.880965541884937	0.0
98-99	1.1428514830212269E-4	3.80950494340409E-5	0.0	9.509305287250006	0.0
100-101	1.5238019773616357E-4	3.80950494340409E-5	0.0	10.194425703796515	0.0
102-103	1.9047524717020448E-4	3.80950494340409E-5	0.0	10.889203215374554	0.0
104-105	1.9047524717020448E-4	3.80950494340409E-5	0.0	11.588171182390335	0.0
106-107	1.9047524717020448E-4	3.80950494340409E-5	0.0	12.28527249198385	0.0
108-109	1.9047524717020448E-4	3.80950494340409E-5	0.0	13.010316520337234	1.904752471702045E-5
110-111	1.9047524717020448E-4	3.80950494340409E-5	0.0	13.794541207986398	3.80950494340409E-5
112-113	1.9047524717020448E-4	3.80950494340409E-5	0.0	14.602670539155426	3.80950494340409E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGGGG	7110	0.0	26.946796	4
GTACATG	13480	0.0	25.519648	1
TACATGG	13585	0.0	24.747679	2
GTATCAA	9045	0.0	24.017124	1
ACATGGG	13525	0.0	23.625141	3
TCAACGC	10170	0.0	21.00363	4
AACGCAG	10380	0.0	20.462496	6
CAACGCA	10530	0.0	20.284397	5
TATCAAC	10685	0.0	20.1595	2
ATGGGGA	3855	0.0	19.755209	5
ATCAACG	10855	0.0	19.35006	3
GTATTAG	850	0.0	18.905212	1
ACGCAGA	11440	0.0	18.358469	7
CGCAGAG	11850	0.0	17.673073	8
GGTATCA	7345	0.0	16.773174	1
GAGTACT	3470	0.0	16.459517	12-13
ATGGGGG	4105	0.0	15.508387	5
GTATTAT	1115	0.0	15.479605	1
GCAGAGT	12820	0.0	15.4077	9
GTATAAT	1315	0.0	15.388272	1
>>END_MODULE