FastQCFastQC Report
Mon 15 Aug 2016
SRR2920957_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920957_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2625013
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39460.15032306506672538No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT32460.12365653046289675No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31250.11904702948137781No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA29280.11154230474287176No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA107400.030.2472741
GGTATCA81300.028.0803321
CATGGGG71050.025.9142864
TCAACGC130750.024.7914564
GTACATG134050.024.4562511
CAACGCA132950.024.3477655
ATCAACG134850.024.3018883
TATCAAC134500.024.2299142
AACGCAG137700.023.9349966
TACATGG136850.023.2043422
ACATGGG137900.022.4249443
GAGTACT72550.021.65284712-13
AGTACTT79450.021.34516512-13
ACGCAGA152250.021.3350097
CGCAGAG157250.020.5431338
ATGGGGA37150.019.7012985
GTACTTT84950.018.87747614-15
GCAGAGT167500.018.6825759
TATACTG10300.018.486785
AGAGTAC153050.018.29030810-11
Produced by FastQC (version 0.11.5)