##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920957_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2625013
Sequences flagged as poor quality	0
Sequence length	125
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.705045270251993	31.0	30.0	33.0	26.0	34.0
2	29.814266062682357	31.0	30.0	34.0	26.0	34.0
3	29.97460774479974	31.0	30.0	34.0	26.0	34.0
4	33.51795362537252	35.0	33.0	37.0	28.0	37.0
5	32.99422745715926	35.0	33.0	37.0	26.0	37.0
6	32.75669834777961	35.0	33.0	37.0	25.0	37.0
7	32.59478524487307	35.0	33.0	37.0	25.0	37.0
8	32.529609186697364	35.0	33.0	37.0	25.0	37.0
9	33.8225894500332	37.0	33.0	39.0	25.0	39.0
10-11	33.72261451657573	37.0	33.0	39.0	24.0	39.0
12-13	33.60569433370425	37.0	33.0	39.0	23.0	39.0
14-15	34.46843482298945	38.0	33.0	41.0	22.0	41.0
16-17	34.45759297192052	38.0	33.0	41.0	22.0	41.0
18-19	34.49776039204377	38.5	33.5	41.0	22.5	41.0
20-21	34.459406296273585	39.0	33.0	41.0	22.0	41.0
22-23	34.411833769966094	39.0	33.0	41.0	22.0	41.0
24-25	34.367632084107775	38.5	33.0	41.0	22.0	41.0
26-27	34.20122947962543	38.0	33.0	41.0	19.5	41.0
28-29	34.07130078974847	38.0	33.0	40.5	18.5	41.0
30-31	33.87708594205057	38.0	33.0	40.0	18.0	41.0
32-33	33.71967072163071	38.0	33.0	40.0	17.0	41.0
34-35	33.575931433482424	38.0	33.0	40.0	16.0	41.0
36-37	33.415328419325924	38.0	32.0	40.0	15.5	41.0
38-39	33.25652958671061	38.0	32.0	40.0	14.0	41.0
40-41	33.04822261832608	38.0	32.0	40.0	9.0	41.0
42-43	32.88173087142807	38.0	32.0	40.0	9.0	41.0
44-45	32.63202429854633	38.0	31.0	40.0	7.5	41.0
46-47	32.448559492848226	38.0	31.0	40.0	4.0	41.0
48-49	32.21423055809628	37.5	31.0	40.0	2.0	41.0
50-51	31.99986152449531	37.0	30.5	40.0	2.0	41.0
52-53	31.751816467194637	37.0	30.0	40.0	2.0	41.0
54-55	31.55123536531057	37.0	30.0	40.0	2.0	41.0
56-57	31.39411252439512	36.0	30.0	40.0	2.0	41.0
58-59	31.313351210070195	36.0	30.0	40.0	2.0	41.0
60-61	31.030367087705848	36.0	29.5	39.5	2.0	41.0
62-63	30.75825548292523	35.0	29.0	39.0	2.0	41.0
64-65	30.477935156892556	35.0	29.0	39.0	2.0	41.0
66-67	30.154968756345205	35.0	29.0	38.5	2.0	40.0
68-69	29.83451948618921	35.0	29.0	37.5	2.0	40.0
70-71	29.480055527344057	35.0	28.0	37.0	2.0	39.5
72-73	29.20103786152678	34.0	28.0	36.5	2.0	39.0
74-75	28.94139990925759	34.0	28.0	36.0	2.0	39.0
76-77	28.61996016781631	34.0	28.0	36.0	2.0	38.5
78-79	28.31108131654967	34.0	27.5	35.0	2.0	37.0
80-81	28.06876042137696	34.0	27.0	35.0	2.0	37.0
82-83	27.828258183864232	34.0	26.5	35.0	2.0	36.0
84-85	27.647420793725594	34.0	26.0	35.0	2.0	36.0
86-87	27.430563772446078	34.0	26.0	35.0	2.0	36.0
88-89	27.310077511997083	34.0	26.0	35.0	2.0	35.0
90-91	27.125979947527878	33.5	26.0	35.0	2.0	35.0
92-93	27.270575612387443	34.0	26.0	35.0	2.0	35.0
94-95	27.21802692024763	34.0	26.0	35.0	2.0	35.0
96-97	27.157812551785458	34.0	26.0	35.0	2.0	35.0
98-99	27.106182902713243	34.0	26.0	35.0	2.0	35.0
100-101	26.971716901973437	34.0	25.0	35.0	2.0	35.0
102-103	25.66731669519351	31.5	23.5	34.0	2.0	35.0
104-105	26.720643859668506	33.0	25.0	35.0	2.0	35.0
106-107	26.912974526221394	34.0	25.0	35.0	2.0	35.0
108-109	26.884387620175595	34.0	25.0	35.0	2.0	35.0
110-111	26.817065667865265	34.0	25.0	35.0	2.0	35.0
112-113	26.698795015491353	34.0	25.0	35.0	2.0	35.0
114-115	26.598382941341626	34.0	25.0	35.0	2.0	35.0
116-117	26.456376596992094	34.0	25.0	35.0	2.0	35.0
118-119	26.31395882610867	34.0	24.5	35.0	2.0	35.0
120-121	26.164516899535357	34.0	24.0	35.0	2.0	35.0
122-123	25.916727079065893	34.0	23.5	35.0	2.0	35.0
124-125	24.79747986009974	32.5	17.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	99762.0
3	30707.0
4	15619.0
5	12585.0
6	14145.0
7	16745.0
8	17988.0
9	19348.0
10	20931.0
11	21800.0
12	22512.0
13	24113.0
14	25935.0
15	21378.0
16	15905.0
17	14257.0
18	13400.0
19	12947.0
20	12845.0
21	13567.0
22	15304.0
23	19252.0
24	25442.0
25	33293.0
26	40920.0
27	48136.0
28	56917.0
29	66727.0
30	78467.0
31	93503.0
32	113381.0
33	139515.0
34	178811.0
35	243956.0
36	384980.0
37	499158.0
38	140517.0
39	245.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.51959657726406	19.583123233205615	13.236746350355922	25.660533839174406
2	16.15866782780687	20.79130485796985	37.02105823758117	26.02896907664211
3	18.602366291789828	24.39245532583275	29.869357561341147	27.135820821036276
4	13.590576955864858	16.45374851768461	34.810658405495886	35.14501612095465
5	13.98184838601518	37.355137623068245	34.03412381503947	14.6288901758771
6	33.602163543499636	35.71389659167231	16.33587344230544	14.348066422522612
7	28.346699673927578	32.04485391124994	21.053977005398732	18.554469409423756
8	26.810642266708935	35.119845895003785	19.486844992806805	18.58266684548048
9	26.66696134550357	14.903559999786612	19.115879658243678	39.31359899646614
10-11	25.480282228142986	25.669653616107617	27.466719502681013	21.383344653068377
12-13	26.07461456760606	23.099862354078653	27.567896589334108	23.25762648898118
14-15	23.445739296511977	24.351830893386957	25.02337821493325	27.17905159516782
16-17	23.2995515623291	27.213024852524715	25.929926040368155	23.557497544778027
18-19	23.487007674983314	26.07171460807888	27.795379765613554	22.645897951324255
20-21	24.11973927345519	25.333390742504434	27.588904356765482	22.95796562727489
22-23	23.777544368106824	25.15255510874094	27.25310878247601	23.816791740676223
24-25	23.532386080024995	25.560693179090315	27.32339974775475	23.583520993129937
26-27	23.48806416582522	25.510468860142126	27.479205166796856	23.522261807235804
28-29	23.422607985280056	25.61945446817477	27.585396879708696	23.37254066683648
30-31	23.854571947832127	25.46398888404322	27.348732660134345	23.33270650799031
32-33	23.356363675160914	25.706075833992543	27.167458477558753	23.77010201328779
34-35	23.532888392915833	25.67923783074494	27.39856945603243	23.3893043203068
36-37	23.743921432188884	25.45418967227464	27.27685497064034	23.525033924896128
38-39	23.7356363888565	25.521918201821077	27.16792687657717	23.574518532745262
40-41	23.71217564664884	25.487117135810884	27.25245528300992	23.54825193453036
42-43	23.879489055545395	25.48398344613133	27.003612584598503	23.632914913724772
44-45	23.749205958464817	25.429392130957883	26.956714590510828	23.864687320066473
46-47	23.777634525228	25.57108827327556	26.77245971212589	23.878817489370547
48-49	23.748114144781095	25.70416376254172	26.558309938376023	23.98941215430116
50-51	23.57417925822933	25.903084103300188	26.53404508709483	23.988691551375652
52-53	23.745124235768255	25.808892906191744	26.35795464072153	24.08802821731847
54-55	23.654965188993092	25.830434003894563	26.216232180079796	24.298368627032545
56-57	23.695683071915525	25.830217315343308	26.164796293777147	24.309303318964023
58-59	23.597610443810105	25.68799578394309	26.40816262761251	24.306231144634303
60-61	23.638702630517848	25.904514435415454	26.382849307174755	24.073933626891943
62-63	23.47279375485841	25.955745603060443	26.379807837985815	24.19165280409533
64-65	23.3642375423541	25.90812278320437	26.33583225937507	24.391807415066463
66-67	23.54448194872174	26.04410370896003	26.173200655336114	24.238213686982114
68-69	23.64535605083697	25.66196510064659	26.250060970219096	24.442617878297344
70-71	23.406386989896927	25.62821711299276	26.386166742821125	24.579229154289187
72-73	23.637642756395575	25.62397030750815	26.159953474953724	24.578433461142556
74-75	23.439420693208067	25.810477758920396	26.139165165993617	24.610936381877917
76-77	23.582715635894484	25.619778815830813	26.260728617209338	24.536776931065365
78-79	23.582371715665136	25.806189780009415	26.129776514992013	24.481661989333432
80-81	23.487202291135112	25.760428383737693	26.096108179620604	24.65626114550659
82-83	23.43296317510622	25.747147594162072	26.100385173396624	24.719504057335083
84-85	23.318036827067555	25.8073150792612	25.95370734996269	24.920940743708552
86-87	23.336133009515976	25.761943178514034	25.9817507858846	24.920173026085386
88-89	23.30052634467116	25.757411201248715	25.939814095001175	25.002248359078948
90-91	23.270868194797636	25.764830833851292	26.0204635763356	24.943837395015468
92-93	23.312486603634287	25.76661506585058	25.969468194050823	24.95143013646431
94-95	23.069500202711147	25.69647663639717	26.098564866042988	25.1354582948487
96-97	23.21231769021019	25.571769337263657	26.07913240762561	25.13678056490054
98-99	23.13449783889097	25.599720616214245	25.90101461430215	25.36476693059264
100-101	23.071661742947168	25.683697160195717	25.95700074417832	25.287640352678793
102-103	22.915873591088136	25.643501048098198	26.00396223416964	25.436663126644028
104-105	22.90400240516974	25.715501799114215	25.806057659295817	25.574438136420223
106-107	22.701588621801864	25.89140540955528	25.918456805493033	25.488549163149827
108-109	22.745223849717533	25.886580218864104	25.93140693398604	25.436788997432323
110-111	22.55694347994654	26.06922094289315	25.722649459071473	25.651186118088837
112-113	22.565272429226884	26.136898984539975	25.67032648835236	25.627502097880782
114-115	22.593641210400442	26.21226166540744	25.612936582414434	25.58116054177768
116-117	22.5085287432172	26.045928339506847	25.694948743720126	25.75059417355583
118-119	22.43829151238513	26.09312096421786	25.59748339016939	25.871104133227625
120-121	22.351284624790203	26.295287216917096	25.619063637972374	25.73436452032033
122-123	22.208107444507114	26.395929610498804	25.407457059931804	25.98850588506228
124-125	22.14573729699542	26.459684036675913	25.33853553658204	26.05604312974662
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	104.0
1	103.5
2	222.5
3	416.5
4	362.5
5	243.0
6	302.5
7	633.5
8	991.5
9	988.0
10	775.5
11	633.0
12	645.0
13	711.5
14	800.5
15	946.5
16	1103.0
17	1225.5
18	1347.0
19	1540.0
20	1827.5
21	2175.5
22	2733.5
23	3484.0
24	4379.5
25	5836.0
26	8248.5
27	11059.5
28	13416.5
29	16477.5
30	20113.5
31	24610.0
32	29629.0
33	33370.0
34	37028.0
35	40480.0
36	44547.0
37	50015.5
38	54808.0
39	57559.5
40	61065.0
41	65983.0
42	70711.0
43	76873.0
44	82701.5
45	88421.5
46	94083.5
47	98453.0
48	103138.5
49	108244.5
50	115784.5
51	118235.5
52	114742.5
53	112591.0
54	109391.0
55	103343.5
56	96454.5
57	90015.5
58	84505.5
59	76479.5
60	64497.5
61	52247.0
62	41052.5
63	30803.5
64	22733.5
65	17124.5
66	12380.0
67	9060.0
68	6736.0
69	5152.0
70	4064.5
71	3104.5
72	2371.5
73	1993.0
74	1508.5
75	1118.5
76	775.5
77	481.0
78	302.5
79	163.5
80	99.0
81	57.0
82	31.0
83	19.5
84	14.5
85	5.5
86	4.0
87	6.5
88	6.0
89	3.5
90	4.0
91	5.0
92	3.5
93	3.5
94	3.5
95	6.0
96	7.0
97	3.0
98	1.5
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.048152142484627694
2	2.6666534603828626E-4
3	0.0023618930649105356
4	0.12457081164931373
5	0.017257057393620528
6	0.002285702966042454
7	0.005180926723029562
8	0.011771370275118638
9	0.0261712989611861
10-11	0.020590374219099105
12-13	0.02001894847758849
14-15	0.029523663311381697
16-17	0.038628380126117474
18-19	0.041847411803293925
20-21	0.021999891048158617
22-23	0.02413321381646491
24-25	0.022209413820045844
26-27	0.021447512831365024
28-29	0.02081894451570335
30-31	0.025809395991562706
32-33	0.024723687082692545
34-35	0.02919985539119235
36-37	0.03177127122799011
38-39	0.03226650687063264
40-41	0.03529506330063889
42-43	0.03249507716723689
44-45	0.029714138558551897
46-47	0.04013313457876209
48-49	0.03321888310648367
50-51	0.022990362333443683
52-53	0.03081889499213909
54-55	0.032114126672896474
56-57	0.025523683120807404
58-59	0.02895223756987108
60-61	0.04262836031669176
62-63	0.05110450881576587
64-65	0.043904544472732135
66-67	0.03870457022498555
68-69	0.029752233607985942
70-71	0.036571247456679265
72-73	0.03999980190574295
74-75	0.02419035639061597
76-77	0.020895134614571435
78-79	0.03171412865383905
80-81	0.02426654648948405
82-83	0.03809504943404089
84-85	0.021237990059477803
86-87	0.03428554449063681
88-89	0.03340935835365387
90-91	0.03565696627026228
92-93	0.02668558212854565
94-95	0.02243798411665009
96-97	0.017618960363243917
98-99	0.02537130292307124
100-101	0.024457021736654257
102-103	0.028037956383454098
104-105	0.02537130292307124
106-107	0.014457071260218522
108-109	0.017599912838526896
110-111	0.022495126690801153
112-113	0.012971364332290927
114-115	0.015009449477012115
116-117	0.01367612274682068
118-119	0.008076150480016671
120-121	0.005390449494916787
122-123	0.003980932665857274
124-125	0.0672568097757992
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2625013.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.71784253892046
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.86933751367233	30.349703481572465
2	13.688045360696572	12.2419971421046
3	6.4427782683965855	8.643214323580114
4	3.552100874305163	6.353691503181599
5	2.168708361538679	4.848997951206342
6	1.4421466263341616	3.8693811452667837
7	0.9948057769117061	3.1139897662122276
8	0.7058887672624331	2.525265819554732
9	0.5413323784439172	2.1786494454426952
>10	2.44608929341326	18.336113655136334
>50	0.10639334287779663	3.2435606265971653
>100	0.040569313740118955	3.150290391834839
>500	9.450164991424112E-4	0.29035842752865715
>1k	8.591059083028086E-4	0.8547863207812425
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3946	0.15032306506672538	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	3246	0.12365653046289675	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3125	0.11904702948137781	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	2928	0.11154230474287176	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.80950494340409E-5	0.0
5	0.0	0.0	0.0	3.80950494340409E-5	0.0
6	0.0	0.0	0.0	3.80950494340409E-5	0.0
7	0.0	0.0	0.0	3.80950494340409E-5	0.0
8	0.0	0.0	0.0	3.80950494340409E-5	0.0
9	0.0	0.0	0.0	3.80950494340409E-5	0.0
10-11	0.0	0.0	0.0	3.80950494340409E-5	0.0
12-13	0.0	0.0	0.0	3.80950494340409E-5	0.0
14-15	0.0	0.0	0.0	5.7142574151061346E-5	0.0
16-17	0.0	0.0	0.0	9.523762358510225E-5	0.0
18-19	0.0	0.0	0.0	1.3333267301914313E-4	0.0
20-21	0.0	0.0	0.0	2.0952277188722495E-4	0.0
22-23	0.0	0.0	0.0	7.809485133978384E-4	0.0
24-25	0.0	0.0	0.0	0.0020952277188722493	0.0
26-27	0.0	0.0	0.0	0.0035237920726487832	0.0
28-29	0.0	0.0	0.0	0.007561867312657119	0.0
30-31	0.0	0.0	0.0	0.020533231644948045	0.0
32-33	0.0	0.0	0.0	0.04777119199028729	0.0
34-35	0.0	0.0	0.0	0.08483767508960907	0.0
36-37	0.0	0.0	0.0	0.14657070269747235	0.0
38-39	0.0	0.0	0.0	0.2442273619216362	0.0
40-41	0.0	0.0	0.0	0.35260777756148254	0.0
42-43	0.0	0.0	0.0	0.44919777540149325	0.0
44-45	0.0	0.0	0.0	0.558835327672663	0.0
46-47	0.0	0.0	0.0	0.6933108521748274	0.0
48-49	0.0	0.0	0.0	0.8322434974607744	0.0
50-51	0.0	0.0	0.0	0.9753856457091832	0.0
52-53	0.0	0.0	0.0	1.121518255338164	0.0
54-55	0.0	0.0	0.0	1.2818412708813252	0.0
56-57	0.0	0.0	0.0	1.4663736903398195	0.0
58-59	0.0	0.0	0.0	1.6666393652145723	0.0
60-61	1.904752471702045E-5	0.0	0.0	1.8753430935389654	0.0
62-63	3.80950494340409E-5	0.0	0.0	2.0795516060301416	0.0
64-65	3.80950494340409E-5	0.0	0.0	2.2979695719602153	0.0
66-67	3.80950494340409E-5	0.0	0.0	2.552977832871685	0.0
68-69	3.80950494340409E-5	0.0	0.0	2.8461001907419123	0.0
70-71	3.80950494340409E-5	0.0	0.0	3.1590129267931246	0.0
72-73	3.80950494340409E-5	0.0	0.0	3.467944730178479	0.0
74-75	3.80950494340409E-5	0.0	0.0	3.7764955830694933	0.0
76-77	3.80950494340409E-5	0.0	0.0	4.121789111139639	0.0
78-79	3.80950494340409E-5	0.0	0.0	4.5121871777396905	0.0
80-81	3.80950494340409E-5	0.0	0.0	4.935613652199056	0.0
82-83	3.80950494340409E-5	0.0	0.0	5.367935320701269	0.0
84-85	3.80950494340409E-5	0.0	0.0	5.800999842667446	0.0
86-87	3.80950494340409E-5	0.0	0.0	6.269454665557847	0.0
88-89	3.80950494340409E-5	0.0	0.0	6.779756900251542	0.0
90-91	3.80950494340409E-5	0.0	0.0	7.330706552691358	0.0
92-93	3.80950494340409E-5	0.0	0.0	7.906017989244244	0.0
94-95	3.80950494340409E-5	0.0	0.0	8.478396106990708	0.0
96-97	3.80950494340409E-5	0.0	0.0	9.07700266627251	0.0
98-99	3.80950494340409E-5	0.0	0.0	9.724561364077054	0.0
100-101	3.80950494340409E-5	0.0	0.0	10.430424535040398	0.0
102-103	3.80950494340409E-5	0.0	0.0	11.146554321826216	0.0
104-105	3.80950494340409E-5	0.0	0.0	11.866207900684682	0.0
106-107	3.80950494340409E-5	0.0	0.0	12.586794808254282	0.0
108-109	3.80950494340409E-5	0.0	0.0	13.33898155932942	0.0
110-111	3.80950494340409E-5	0.0	0.0	14.15278705286412	0.0
112-113	3.80950494340409E-5	0.0	0.0	14.992859082983589	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	10740	0.0	30.247274	1
GGTATCA	8130	0.0	28.080332	1
CATGGGG	7105	0.0	25.914286	4
TCAACGC	13075	0.0	24.791456	4
GTACATG	13405	0.0	24.456251	1
CAACGCA	13295	0.0	24.347765	5
ATCAACG	13485	0.0	24.301888	3
TATCAAC	13450	0.0	24.229914	2
AACGCAG	13770	0.0	23.934996	6
TACATGG	13685	0.0	23.204342	2
ACATGGG	13790	0.0	22.424944	3
GAGTACT	7255	0.0	21.652847	12-13
AGTACTT	7945	0.0	21.345165	12-13
ACGCAGA	15225	0.0	21.335009	7
CGCAGAG	15725	0.0	20.543133	8
ATGGGGA	3715	0.0	19.701298	5
GTACTTT	8495	0.0	18.877476	14-15
GCAGAGT	16750	0.0	18.682575	9
TATACTG	1030	0.0	18.48678	5
AGAGTAC	15305	0.0	18.290308	10-11
>>END_MODULE