FastQCFastQC Report
Mon 15 Aug 2016
SRR2920956_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920956_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3325130
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG68300.026.4823424
TACATGG130250.024.8974552
GTACATG136800.024.8827511
GTATCAA97100.023.3503951
ACATGGG143300.022.3391023
GGTATCA77400.020.2210271
TCAACGC107400.019.9989154
AACGCAG106650.019.970696
TATCAAC110400.019.9420052
CAACGCA109350.019.7508075
ATCAACG110450.019.6094443
GTATAGC11300.018.9588721
ACGCAGA117050.018.1962747
TACGACC3655.516449E-817.9309524
CGCAGAG118000.017.797428
GAGTACT39650.016.42941912-13
ATGGGGG38500.016.3810635
ATGGGGA41500.016.200465
GTATAAT20950.016.191211
ATGGGGC32400.016.159755
Produced by FastQC (version 0.11.5)