##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2920956_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3325130 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.62980966157714 31.0 28.0 31.0 12.0 34.0 2 27.72075016615892 31.0 28.0 33.0 12.0 34.0 3 27.79383182010929 31.0 27.0 34.0 12.0 34.0 4 31.161292340449847 35.0 32.0 37.0 16.0 37.0 5 31.226835341776113 35.0 32.0 37.0 16.0 37.0 6 31.172417619762236 35.0 32.0 37.0 13.0 37.0 7 31.0384712778147 35.0 32.0 37.0 11.0 37.0 8 31.01131805373023 35.0 32.0 37.0 11.0 37.0 9 32.099612646723585 37.0 32.0 39.0 10.0 39.0 10-11 32.018761822846024 37.0 31.5 39.0 10.0 39.0 12-13 31.94634480456403 37.0 31.0 39.0 10.0 39.0 14-15 32.7367528487608 38.0 31.0 40.0 10.0 41.0 16-17 32.598716591531755 38.0 31.0 40.0 10.0 41.0 18-19 32.70132536171518 38.0 31.0 40.0 10.0 41.0 20-21 32.71216508828226 38.0 31.0 40.0 9.0 41.0 22-23 32.643685359670144 38.0 31.0 40.0 8.0 41.0 24-25 32.548243527320736 38.0 31.0 40.0 8.0 41.0 26-27 32.372272813393764 38.0 30.5 40.0 5.0 41.0 28-29 32.26705136340534 38.0 30.0 40.0 2.0 41.0 30-31 32.11786185803262 38.0 30.0 40.0 2.0 41.0 32-33 31.990609540078133 38.0 30.0 40.0 2.0 41.0 34-35 31.791116738292942 37.0 30.0 40.0 2.0 41.0 36-37 31.639037721833432 37.0 30.0 40.0 2.0 41.0 38-39 31.496571111505418 37.0 30.0 40.0 2.0 41.0 40-41 31.411796982373623 37.0 30.0 40.0 2.0 41.0 42-43 31.20053261075507 37.0 30.0 40.0 2.0 41.0 44-45 31.040647583703496 37.0 29.0 40.0 2.0 41.0 46-47 30.84742897270182 37.0 29.0 40.0 2.0 41.0 48-49 30.67726585125995 36.5 28.5 40.0 2.0 41.0 50-51 30.4512751381149 36.0 28.0 40.0 2.0 41.0 52-53 30.278168973844632 36.0 28.0 40.0 2.0 41.0 54-55 30.070907753982553 36.0 28.0 40.0 2.0 41.0 56-57 29.877001951803386 35.5 27.5 39.0 2.0 41.0 58-59 29.63077353366635 35.0 27.0 39.0 2.0 41.0 60-61 29.44366671378262 35.0 26.5 39.0 2.0 40.5 62-63 29.33504990782315 35.0 26.5 39.0 2.0 41.0 64-65 29.126534902394795 35.0 26.0 39.0 2.0 40.0 66-67 28.881571397208532 34.5 26.0 38.5 2.0 40.0 68-69 28.577934095809788 34.0 26.0 38.0 2.0 40.0 70-71 28.255584142574875 34.0 26.0 37.0 2.0 40.0 72-73 27.9742154442082 34.0 26.0 37.0 2.0 39.0 74-75 27.66811282566396 34.0 25.5 36.0 2.0 39.0 76-77 27.381759059044306 34.0 25.0 36.0 2.0 39.0 78-79 27.04532800221345 33.0 25.0 35.5 2.0 37.5 80-81 26.70876807824055 33.0 24.0 35.0 2.0 37.0 82-83 26.411391735059983 33.0 23.5 35.0 2.0 37.0 84-85 26.14144680057622 33.0 23.0 35.0 2.0 36.0 86-87 26.042269625548474 33.0 22.5 35.0 2.0 36.0 88-89 26.098993573183606 33.0 23.0 35.0 2.0 36.0 90-91 25.983432226709933 33.0 23.0 35.0 2.0 35.0 92-93 25.805548805610606 33.0 21.5 35.0 2.0 35.0 94-95 25.69828412723713 33.0 20.5 35.0 2.0 35.0 96-97 25.578115592473075 33.0 20.0 35.0 2.0 35.0 98-99 25.488045880912928 33.0 20.0 35.0 2.0 35.0 100-101 25.2923506750112 33.0 19.0 35.0 2.0 35.0 102-103 24.01811117159329 30.5 17.5 34.0 2.0 35.0 104-105 24.957742855166565 32.0 18.0 35.0 2.0 35.0 106-107 25.11964524695275 33.0 18.0 35.0 2.0 35.0 108-109 25.08372514758821 33.0 18.0 35.0 2.0 35.0 110-111 24.948847864594768 33.0 16.0 35.0 2.0 35.0 112-113 24.82459467750133 33.0 10.0 35.0 2.0 35.0 114-115 24.658472300331113 33.0 7.0 35.0 2.0 35.0 116-117 24.487886939758745 33.0 2.0 35.0 2.0 35.0 118-119 24.248669224962633 32.5 2.0 35.0 2.0 35.0 120-121 23.98171379765603 32.0 2.0 35.0 2.0 35.0 122-123 23.70111830214157 32.0 2.0 35.0 2.0 35.0 124-125 22.535241479280508 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 243166.0 3 34070.0 4 21833.0 5 20336.0 6 23880.0 7 24786.0 8 25122.0 9 26473.0 10 27131.0 11 28021.0 12 28187.0 13 28484.0 14 28857.0 15 25822.0 16 22834.0 17 20655.0 18 19316.0 19 18947.0 20 19147.0 21 21018.0 22 24504.0 23 32239.0 24 41840.0 25 51896.0 26 59990.0 27 68966.0 28 79034.0 29 91148.0 30 104505.0 31 122534.0 32 146368.0 33 177471.0 34 223391.0 35 294102.0 36 424557.0 37 520708.0 38 153647.0 39 145.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.814266283950815 18.949488908270038 12.36442764254596 24.871817165233185 2 15.644972856166559 20.82125135959804 38.95218305724269 24.581592726992714 3 18.643905661433056 24.824939641101444 29.86073383666007 26.670420860805432 4 12.663857917310144 16.57794820919668 37.41378075992653 33.344413113566645 5 13.473105494463846 38.095458673085886 34.87320734127193 13.55822849117834 6 32.007713774687886 37.06115650679813 17.29539083315564 13.635738885358354 7 27.477577865057263 33.23718154041969 21.577911975244145 17.7073286192789 8 25.415993720168544 36.05321014276992 20.48052139443537 18.05027474262616 9 25.908963637244014 16.431495361084718 19.840885311169277 37.81865569050199 10-11 24.34700005594049 26.773719519200522 28.40041480173307 20.47886562312592 12-13 24.861764211830057 24.49243866893625 28.020197636310513 22.625599482923185 14-15 22.420520528919795 26.086881334696322 25.345897990430792 26.14670014595309 16-17 22.354976698920215 28.82723487286923 26.308604008859387 22.509184419351165 18-19 22.381651243710774 28.14413271060079 27.65317446235185 21.82104158333659 20-21 23.352736477987136 27.367501695124762 27.278794301252958 22.000967525635144 22-23 22.929661901592645 27.15695211297595 26.91422235200958 22.99916363342182 24-25 22.522611526841207 27.25847605196155 27.213259013182867 23.00565340801438 26-27 22.53389730982372 27.415359542531238 27.25507766054917 22.795665487095874 28-29 22.596260843517708 27.624233540288984 27.257590011272086 22.521915604921222 30-31 22.945126482556976 27.470457128438596 27.105278274256612 22.479138114747812 32-33 22.579139034906614 27.58777178268873 26.974006121266275 22.85908306113838 34-35 22.789326333971403 27.43459941119582 27.097488942815733 22.67858531201704 36-37 22.855260478684418 27.30022157583822 27.076735860366618 22.767782085110746 38-39 22.77435280679705 27.569923692268087 26.9975931471308 22.65813035380406 40-41 22.84495974475312 27.415777205730546 27.107364918500814 22.631898131015525 42-43 22.83008543193933 27.355318101757735 27.035367496585383 22.77922896971755 44-45 22.9019471624119 27.41396862345286 26.94516998762441 22.738914226510822 46-47 22.69038302137459 27.402575154289817 26.957916064354944 22.949125759980653 48-49 22.712466214397487 27.36581924089875 27.079729961795618 22.841984582908147 50-51 22.579507064621072 27.5341711341893 26.904527156441894 22.981794644747733 52-53 22.688436230149104 27.437072386886236 26.75114708094159 23.123344302023067 54-55 22.833799806954655 27.242340176333197 26.763305625607213 23.160554391104938 56-57 22.702112356995958 27.296541828734533 26.7756440397946 23.22570177447491 58-59 22.683863563120713 27.1023005906859 26.951256795955686 23.262579050237704 60-61 22.8008330576524 27.086583862139484 26.917235571862502 23.195347508345613 62-63 22.64427231387024 27.173156854211328 26.96880986375764 23.21376096816079 64-65 22.59948435127637 27.02287755087309 26.969129051740527 23.408509046110016 66-67 22.623899384309322 27.147289840765502 26.830174181290793 23.39863659363438 68-69 22.8267883311015 26.907895280966798 26.89810578520102 23.367210602730683 70-71 22.544630907403388 26.625052858128484 27.170807395506323 23.65950883896181 72-73 22.725306032890046 26.582710805404485 27.132191680802098 23.55979148090337 74-75 22.52927558940267 26.787666170689 27.11932711444002 23.563731125468315 76-77 22.57738161204693 26.608784619870317 27.2364959141775 23.57733785390525 78-79 22.603778930150213 26.590836844734373 27.249940678850482 23.555443546264936 80-81 22.639258879974015 26.581327486659646 27.12719748388518 23.652216149481163 82-83 22.53925118729851 26.525338182524656 27.31815870876296 23.617251921413867 84-85 22.634649374349454 26.518789365369273 27.022999916694207 23.823561343587063 86-87 22.614176278862153 26.37168546193282 27.197244417066013 23.816893842139013 88-89 22.505977209913596 26.333797475587424 27.29013602475693 23.870089289742054 90-91 22.48533246880222 26.353031540398657 27.298306690908426 23.863329299890694 92-93 22.507157982720383 26.256739346565166 27.32933517946802 23.906767491246423 94-95 22.321589560609688 26.284813970838243 27.420242334396082 23.973354134155983 96-97 22.44514249889961 26.199418308004425 27.39257713373908 23.962862059356883 98-99 22.359001487471378 26.22347979282465 27.24092104131417 24.176597678389804 100-101 22.340807446008363 26.337806110025824 27.295237702771786 24.026148741194028 102-103 22.035034067415406 26.30359670339784 27.498813490299494 24.162555738887264 104-105 21.985240953346832 26.392894050785653 27.293333646126953 24.32853134974056 106-107 21.877301578635723 26.470780657873423 27.40811394969656 24.24380381379429 108-109 21.88496540751162 26.425608291670205 27.439764856798902 24.24966144401927 110-111 21.804233087533262 26.602297810540428 27.337547249383494 24.25592185254282 112-113 21.74992402350467 26.64893054718264 27.266757422426156 24.33438800688653 114-115 21.714712920369315 26.646040573706575 27.29171388424713 24.34753262167698 116-117 21.706273843593696 26.55972385530073 27.336087582165426 24.39791471894015 118-119 21.73224377423624 26.313405585972856 27.383871684220544 24.57047895557036 120-121 21.769006201923663 26.4686390130338 27.34739900726513 24.41495577777741 122-123 21.667472466823718 26.496498716860373 27.298624525448872 24.537404290867038 124-125 21.650570612626012 26.508921375055237 27.12385087203656 24.71665714028219 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1463.0 1 998.0 2 429.5 3 284.0 4 240.5 5 234.5 6 226.0 7 239.5 8 295.5 9 357.5 10 395.5 11 427.5 12 472.5 13 540.5 14 675.0 15 804.0 16 934.0 17 1086.0 18 1348.0 19 1755.5 20 2171.5 21 2713.5 22 3438.5 23 4507.5 24 6146.5 25 8222.5 26 11362.0 27 16049.5 28 21152.0 29 26988.0 30 33981.0 31 41987.0 32 49680.0 33 57019.5 34 64307.5 35 71669.5 36 80034.5 37 86898.0 38 91125.0 39 94756.0 40 100071.5 41 106206.0 42 111474.5 43 116650.5 44 120793.0 45 123905.5 46 128467.5 47 133665.0 48 137458.5 49 139251.0 50 141104.0 51 137498.5 52 126703.5 53 118264.0 54 111908.5 55 104113.5 56 94455.0 57 83896.0 58 75743.5 59 67868.5 60 56714.5 61 45653.5 62 36793.5 63 28140.5 64 20725.5 65 15758.5 66 12069.5 67 9383.0 68 7449.5 69 5889.5 70 4731.5 71 3709.5 72 2919.5 73 2396.0 74 1827.5 75 1348.5 76 984.0 77 697.0 78 481.0 79 313.0 80 237.0 81 185.0 82 117.0 83 81.0 84 56.5 85 44.5 86 40.0 87 30.0 88 25.0 89 19.0 90 15.5 91 11.5 92 13.0 93 14.0 94 9.0 95 7.0 96 6.5 97 6.5 98 6.0 99 9.5 100 17.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03509637217191509 2 0.018585739504921612 3 0.02550276229801542 4 0.022735953180777896 5 0.01590915242411575 6 0.005082508052316752 7 0.0053832481737556125 8 0.006014802428777221 9 0.0032179192993958133 10-11 0.005052434040172866 12-13 0.008586130467079484 14-15 0.002676587080805863 16-17 0.0020901438440000843 18-19 0.0 20-21 0.004616360864086517 22-23 9.924424007482415E-4 24-25 0.001684144680057622 26-27 0.0015187376132662482 28-29 0.003067549238676383 30-31 0.001413478570762647 32-33 3.0074012143886107E-5 34-35 0.0076839101027629 36-37 0.009292869752460806 38-39 0.0012330344978993303 40-41 0.0010676274311079567 42-43 0.002571328038302262 44-45 0.0010525904250360137 46-47 0.023623136539022534 48-49 0.0035788074451224465 50-51 0.011849160784691126 52-53 0.0017894037225612233 54-55 1.3533305464748748E-4 56-57 1.6540706679137358E-4 58-59 0.008014724236345647 60-61 9.022203643165832E-4 62-63 0.01181908677254724 64-65 0.011608568687540037 66-67 7.518503035971526E-5 68-69 0.004135176669784339 70-71 0.005774210331626132 72-73 0.013428046422245146 74-75 0.0014285155768345899 76-77 0.0010375534189640706 78-79 2.7066610929497495E-4 80-81 0.004887026973381492 82-83 0.001187923479683501 84-85 9.322943764604693E-4 86-87 0.01605952248483518 88-89 0.0 90-91 4.0599916394246246E-4 92-93 0.010390571195712649 94-95 0.007864354175626217 96-97 0.005247915119108125 98-99 7.217762914532665E-4 100-101 0.018360184413842465 102-103 0.007578651060259299 104-105 0.007638799084547071 106-107 0.01941277483887848 108-109 0.010450719220000421 110-111 0.022495361083626805 112-113 0.0026615500747339204 114-115 0.016194855539482668 116-117 0.006210283507712481 118-119 0.005308063143395898 120-121 0.008255316333496736 122-123 0.008345538369928395 124-125 0.0572458821158872 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3325130.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.521617615255906 #Duplication Level Percentage of deduplicated Percentage of total 1 70.72907302672868 35.02618108711169 2 12.676652312096659 12.555366568824008 3 5.972986634196755 8.873758803591784 4 3.272269131752147 6.481922427073412 5 1.987778007756692 4.9218991202071045 6 1.2998024272015518 3.862099126515406 7 0.8768954496198281 3.0397696801241723 8 0.642659465461966 2.546042904434579 9 0.453116509866279 2.019515628308148 >10 2.0033997556531546 16.15101429157039 >50 0.06330802565255439 2.1164516994167806 >100 0.020771322198463955 1.7120775559787669 >500 7.972911253206501E-4 0.2668756965965057 >1k 4.906406900665122E-4 0.42702541024728125 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 3.0074012143886103E-5 0.0 0.0 0.0 5 0.0 3.0074012143886103E-5 0.0 0.0 0.0 6 0.0 3.0074012143886103E-5 0.0 0.0 0.0 7 0.0 3.0074012143886103E-5 0.0 0.0 0.0 8 0.0 3.0074012143886103E-5 0.0 0.0 0.0 9 0.0 3.0074012143886103E-5 0.0 0.0 0.0 10-11 0.0 3.0074012143886103E-5 0.0 3.0074012143886103E-5 0.0 12-13 0.0 3.0074012143886103E-5 0.0 6.0148024287772207E-5 0.0 14-15 0.0 3.0074012143886103E-5 0.0 6.0148024287772207E-5 0.0 16-17 0.0 3.0074012143886103E-5 0.0 7.518503035971526E-5 0.0 18-19 0.0 3.0074012143886103E-5 0.0 1.0525904250360136E-4 0.0 20-21 0.0 3.0074012143886103E-5 0.0 2.7066610929497495E-4 0.0 22-23 0.0 3.0074012143886103E-5 0.0 6.465912610935513E-4 0.0 24-25 0.0 4.5111018215829157E-5 0.0 0.0013382935404029316 0.0 26-27 0.0 6.0148024287772207E-5 0.0 0.0024059209715108884 0.0 28-29 0.0 6.0148024287772207E-5 0.0 0.006044876440921106 0.0 30-31 0.0 6.0148024287772207E-5 0.0 0.020781142391425297 3.0074012143886103E-5 32-33 0.0 6.0148024287772207E-5 0.0 0.054118184852923044 3.0074012143886103E-5 34-35 0.0 6.0148024287772207E-5 0.0 0.10368015686604734 3.0074012143886103E-5 36-37 0.0 6.0148024287772207E-5 0.0 0.18545139588527365 3.0074012143886103E-5 38-39 0.0 6.0148024287772207E-5 0.0 0.30737144111658793 3.0074012143886103E-5 40-41 0.0 6.0148024287772207E-5 0.0 0.43984746461040625 3.0074012143886103E-5 42-43 0.0 6.0148024287772207E-5 0.0 0.5632110624246269 3.0074012143886103E-5 44-45 0.0 6.0148024287772207E-5 0.0 0.7086790591646042 3.0074012143886103E-5 46-47 0.0 6.0148024287772207E-5 0.0 0.8819955911498196 3.0074012143886103E-5 48-49 0.0 1.2029604857554441E-4 0.0 1.0582894503372802 3.0074012143886103E-5 50-51 0.0 1.2029604857554441E-4 0.0 1.2374253036723375 3.0074012143886103E-5 52-53 0.0 1.2029604857554441E-4 0.0 1.418696411869611 3.0074012143886103E-5 54-55 0.0 1.2029604857554441E-4 0.0 1.6250793202070295 3.0074012143886103E-5 56-57 0.0 1.2029604857554441E-4 0.0 1.864227864775212 3.0074012143886103E-5 58-59 0.0 1.2029604857554441E-4 0.0 2.1211351135143586 3.0074012143886103E-5 60-61 0.0 1.2029604857554441E-4 0.0 2.3800723580732184 6.0148024287772207E-5 62-63 0.0 1.2029604857554441E-4 0.0 2.6408591543789264 6.0148024287772207E-5 64-65 0.0 1.2029604857554441E-4 0.0 2.9249533100961465 6.0148024287772207E-5 66-67 0.0 1.2029604857554441E-4 0.0 3.255767443678894 6.0148024287772207E-5 68-69 0.0 1.2029604857554441E-4 0.0 3.6230763909982464 6.0148024287772207E-5 70-71 0.0 1.2029604857554441E-4 0.0 4.014910695220939 6.0148024287772207E-5 72-73 0.0 1.2029604857554441E-4 0.0 4.402369230676696 6.0148024287772207E-5 74-75 0.0 1.2029604857554441E-4 0.0 4.80095515062569 6.0148024287772207E-5 76-77 0.0 1.2029604857554441E-4 0.0 5.2459603083187725 6.0148024287772207E-5 78-79 0.0 1.2029604857554441E-4 0.0 5.740211660897469 6.0148024287772207E-5 80-81 0.0 1.2029604857554441E-4 0.0 6.2602815529016915 6.0148024287772207E-5 82-83 0.0 1.2029604857554441E-4 0.0 6.790411201968043 9.022203643165831E-5 84-85 0.0 1.2029604857554441E-4 0.0 7.3320140866672885 9.022203643165831E-5 86-87 0.0 1.2029604857554441E-4 0.0 7.914502590876147 9.022203643165831E-5 88-89 0.0 1.2029604857554441E-4 0.0 8.565950804930935 9.022203643165831E-5 90-91 0.0 1.2029604857554441E-4 0.0 9.243337854459826 9.022203643165831E-5 92-93 1.5037006071943052E-5 1.2029604857554441E-4 0.0 9.925326227846732 9.022203643165831E-5 94-95 3.0074012143886103E-5 1.2029604857554441E-4 0.0 10.622201237244859 9.022203643165831E-5 96-97 3.0074012143886103E-5 1.2029604857554441E-4 0.0 11.358157425423968 9.022203643165831E-5 98-99 6.0148024287772207E-5 1.2029604857554441E-4 0.0 12.154667637054793 9.022203643165831E-5 100-101 6.0148024287772207E-5 1.2029604857554441E-4 0.0 13.001070634832322 9.022203643165831E-5 102-103 6.0148024287772207E-5 1.2029604857554441E-4 0.0 13.850766736939608 9.022203643165831E-5 104-105 6.0148024287772207E-5 1.2029604857554441E-4 0.0 14.69873358334862 1.0525904250360136E-4 106-107 6.0148024287772207E-5 1.2029604857554441E-4 0.0 15.560098402167736 1.2029604857554441E-4 108-109 6.0148024287772207E-5 1.2029604857554441E-4 0.0 16.46479987248619 1.2029604857554441E-4 110-111 6.0148024287772207E-5 1.2029604857554441E-4 0.0 17.411229034654284 1.2029604857554441E-4 112-113 6.0148024287772207E-5 1.2029604857554441E-4 0.0 18.3740485334408 1.2029604857554441E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATGGGG 6830 0.0 26.482342 4 TACATGG 13025 0.0 24.897455 2 GTACATG 13680 0.0 24.882751 1 GTATCAA 9710 0.0 23.350395 1 ACATGGG 14330 0.0 22.339102 3 GGTATCA 7740 0.0 20.221027 1 TCAACGC 10740 0.0 19.998915 4 AACGCAG 10665 0.0 19.97069 6 TATCAAC 11040 0.0 19.942005 2 CAACGCA 10935 0.0 19.750807 5 ATCAACG 11045 0.0 19.609444 3 GTATAGC 1130 0.0 18.958872 1 ACGCAGA 11705 0.0 18.196274 7 TACGACC 365 5.516449E-8 17.930952 4 CGCAGAG 11800 0.0 17.79742 8 GAGTACT 3965 0.0 16.429419 12-13 ATGGGGG 3850 0.0 16.381063 5 ATGGGGA 4150 0.0 16.20046 5 GTATAAT 2095 0.0 16.19121 1 ATGGGGC 3240 0.0 16.15975 5 >>END_MODULE