FastQCFastQC Report
Mon 15 Aug 2016
SRR2920956_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920956_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3325130
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42070.12652136908932884No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34330.10324408368996099No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA115500.033.7421651
TCAACGC133800.028.2292354
CAACGCA134350.028.0802565
ATCAACG136600.027.7801823
TATCAAC138000.027.7091882
AACGCAG137850.027.621316
GGTATCA82350.027.2083031
CATGGGG69150.025.2430274
ACGCAGA156550.024.2086457
CGCAGAG159350.023.6723258
TACATGG133100.023.1355762
GTACATG135850.022.8974971
TACCGTA2755.527545E-821.6347277
ACATGGG142200.021.5332643
GAGTACT83750.021.20813412-13
GCAGAGT174750.020.9055429
AGTACTT94700.020.32671712-13
AGAGTAC155250.019.37342310-11
CCCTATA8400.019.1448652
GTATATA18350.018.5104061
Produced by FastQC (version 0.11.5)