##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920956_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3325130
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.133641992944636	31.0	30.0	34.0	26.0	34.0
2	30.197022973537877	31.0	30.0	34.0	26.0	34.0
3	30.329419601639636	31.0	30.0	34.0	26.0	34.0
4	33.90471109400234	35.0	35.0	37.0	30.0	37.0
5	33.481851235891526	35.0	35.0	37.0	28.0	37.0
6	33.28427941163203	35.0	33.0	37.0	27.0	37.0
7	33.130416555142205	35.0	33.0	37.0	26.0	37.0
8	33.071978238444814	35.0	33.0	37.0	26.0	37.0
9	34.400942218800466	38.0	34.0	39.0	27.0	39.0
10-11	34.32409950889138	38.0	34.0	39.0	26.5	39.0
12-13	34.229124876320626	38.0	34.0	39.0	26.5	39.0
14-15	35.143452286076034	39.0	34.0	41.0	25.5	41.0
16-17	35.14728642188426	39.0	34.0	41.0	25.5	41.0
18-19	35.20406781088258	39.0	34.0	41.0	25.5	41.0
20-21	35.17923509757513	39.0	34.0	41.0	25.0	41.0
22-23	35.140083545605734	39.0	34.0	41.0	25.0	41.0
24-25	35.11963652548923	39.0	34.0	41.0	25.0	41.0
26-27	34.97300526595953	39.0	34.0	41.0	25.0	41.0
28-29	34.84726612192606	39.0	34.0	41.0	24.0	41.0
30-31	34.67716826109054	38.5	34.0	41.0	24.0	41.0
32-33	34.53144087599583	38.0	33.5	41.0	24.0	41.0
34-35	34.39747167779906	38.0	33.0	40.0	23.0	41.0
36-37	34.243878585198175	38.0	33.0	40.0	22.5	41.0
38-39	34.096739826713545	38.0	33.0	40.0	21.0	41.0
40-41	33.91282446099852	38.0	33.0	40.0	19.0	41.0
42-43	33.75310123213228	38.0	33.0	40.0	18.0	41.0
44-45	33.535854538018064	38.0	33.0	40.0	15.5	41.0
46-47	33.3777855301898	38.0	32.5	40.0	14.5	41.0
48-49	33.164917311503615	38.0	32.0	40.0	11.0	41.0
50-51	32.976215967496	38.0	32.0	40.0	9.0	41.0
52-53	32.74527943268384	38.0	31.5	40.0	8.0	41.0
54-55	32.58033550567948	37.0	31.0	40.0	8.0	41.0
56-57	32.46433793565966	37.0	31.0	40.0	7.0	41.0
58-59	32.41401870002075	37.0	31.0	40.0	4.0	41.0
60-61	32.16558856345466	37.0	31.0	40.0	2.0	41.0
62-63	31.925531182239492	36.0	31.0	40.0	2.0	41.0
64-65	31.673255632110624	36.0	31.0	39.0	2.0	41.0
66-67	31.36751781133369	35.5	30.0	39.0	2.0	41.0
68-69	31.052607867963058	35.0	30.0	39.0	2.0	40.5
70-71	30.7043640399022	35.0	30.0	38.0	2.0	40.0
72-73	30.415474582948637	35.0	29.5	37.5	2.0	40.0
74-75	30.146019403752636	35.0	29.0	37.0	2.0	39.0
76-77	29.802150291868287	35.0	29.0	36.5	2.0	39.0
78-79	29.459312417860353	34.0	29.0	36.0	2.0	38.5
80-81	29.18260233434482	34.0	29.0	35.5	2.0	37.0
82-83	28.90874116199968	34.0	29.0	35.0	2.0	37.0
84-85	28.688969153085743	34.0	29.0	35.0	2.0	36.5
86-87	28.442926441973697	34.0	29.0	35.0	2.0	36.0
88-89	28.296271724714522	34.0	28.5	35.0	2.0	36.0
90-91	28.093307479707562	34.0	27.5	35.0	2.0	35.5
92-93	28.217302631776803	34.0	29.0	35.0	2.0	35.0
94-95	28.15345490251509	34.0	29.0	35.0	2.0	35.0
96-97	28.084935175466825	34.0	29.0	35.0	2.0	35.0
98-99	28.025414344702313	34.0	29.0	35.0	2.0	35.0
100-101	27.882961718789943	34.0	28.0	35.0	2.0	35.0
102-103	26.560063365943584	32.0	24.5	34.0	2.0	35.0
104-105	27.628946838168734	34.0	27.0	35.0	2.0	35.0
106-107	27.817754794549387	34.0	28.0	35.0	2.0	35.0
108-109	27.78558417264889	34.0	28.0	35.0	2.0	35.0
110-111	27.71457476850529	34.0	27.5	35.0	2.0	35.0
112-113	27.593409881718912	34.0	27.0	35.0	2.0	35.0
114-115	27.488530072508443	34.0	27.0	35.0	2.0	35.0
116-117	27.34175941993245	34.0	27.0	35.0	2.0	35.0
118-119	27.194050157437452	34.0	27.0	35.0	2.0	35.0
120-121	27.03028347763847	34.0	27.0	35.0	2.0	35.0
122-123	26.776437462595446	34.0	26.0	35.0	2.0	35.0
124-125	25.642964786339178	32.5	22.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	95377.0
3	30730.0
4	15327.0
5	12435.0
6	14246.0
7	17214.0
8	19273.0
9	21014.0
10	23053.0
11	24519.0
12	25516.0
13	27532.0
14	30437.0
15	25467.0
16	18620.0
17	16934.0
18	15707.0
19	15222.0
20	15405.0
21	16014.0
22	18124.0
23	22834.0
24	29822.0
25	39730.0
26	48984.0
27	57757.0
28	68385.0
29	82463.0
30	96692.0
31	115781.0
32	140743.0
33	174998.0
34	223137.0
35	302721.0
36	473607.0
37	691671.0
38	257103.0
39	536.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.31277360984609	20.060203569185813	13.228476577086475	25.398546243881626
2	15.578586289465468	21.58486214941888	38.046213041205704	24.790338519909945
3	18.373561372420934	24.902948652709558	30.545670974579586	26.17781900028992
4	13.038350019015866	16.938665324063187	36.85187588586385	33.1711087710571
5	13.202207225972955	37.93953139311212	35.11573799042573	13.742523390489197
6	31.9807353736386	37.28698867809223	16.989808899574083	13.74246704869509
7	27.18494839227943	32.96695596103083	21.79599499186012	18.05210065482963
8	25.632234555628912	35.852987663752664	20.34610069012604	18.168677090492384
9	25.903660668383555	15.411724041875575	20.149845272392486	38.534770017348386
10-11	24.61021360231425	26.232467395348824	28.600348508880717	20.55697049345621
12-13	25.23997973228752	23.766464696415206	28.51302880328302	22.480526768014254
14-15	22.587503508382966	25.260312239315493	26.044385068081432	26.107799184220116
16-17	22.286314275700125	28.22935047001006	26.929658695524637	22.55467655876517
18-19	22.267198316595668	27.281006552556292	28.745861966982382	21.705933163865655
20-21	23.124766124054418	26.461055368588486	28.502829974142795	21.911348533214294
22-23	22.68356777458986	26.35763055603518	28.150608327983928	22.80819334139103
24-25	22.379611579956965	26.58746279234266	28.361052232775286	22.671873394925093
26-27	22.364645005455785	26.807000551218586	28.415912093795896	22.412442349529734
28-29	22.370374871238543	26.84604709665009	28.46352710055448	22.320050931556885
30-31	22.754549739780664	26.70572939776092	28.312453317394194	22.227267545064226
32-33	22.373147956937558	26.82680493590373	28.204433637940575	22.595613469218133
34-35	22.562821742155037	26.764765826235738	28.268847650741723	22.403564780867505
36-37	22.695543141884766	26.620966254757285	28.17835915927046	22.50513144408749
38-39	22.65060737520263	26.786300105126248	28.072814328456268	22.490278191214852
40-41	22.698416709611564	26.679025170373166	28.115731310245074	22.506826809770196
42-43	22.769258307173253	26.539032545798317	28.007556966804398	22.684152180224032
44-45	22.82886010925378	26.65466774281952	27.845831176164275	22.670640971762417
46-47	22.64858334565608	26.690418600760967	27.699956029237022	22.961042024345932
48-49	22.741312218623882	26.73494651879551	27.67575242963059	22.847988832950016
50-51	22.583612958147274	26.999499156989465	27.440225965927635	22.976661918935626
52-53	22.608143955688913	26.930969088490414	27.34035390871728	23.120533047103393
54-55	22.68353825664716	26.82053639358032	27.260777313724148	23.235148036048372
56-57	22.602825334365928	26.90186202298248	27.19596777019743	23.299344872454157
58-59	22.434593088842693	26.807131962974438	27.491103095791757	23.267171852391108
60-61	22.69672234724947	26.82859867858905	27.299050461531056	23.175628512630425
62-63	22.457889379376667	27.007440162313305	27.290895692998447	23.243774765311578
64-65	22.342278291958745	26.89605808744739	27.279578936410363	23.482084684183505
66-67	22.501817545097907	27.015737616436837	27.07271970483639	23.409725133628864
68-69	22.640315782238048	26.72765797497405	27.154784647505803	23.4772415952821
70-71	22.421553669142188	26.560957922131372	27.27938146981696	23.73810693890948
72-73	22.641700165817223	26.469516211743617	27.173460940740085	23.715322681699078
74-75	22.46695385918582	26.683952162923045	27.074868689287314	23.774225288603816
76-77	22.46812426153687	26.650758376066936	27.135481965256435	23.745635397139754
78-79	22.49278448271194	26.686132669251034	27.05572298670973	23.765359861327294
80-81	22.40494485156707	26.69932270898957	26.967194905825853	23.928537533617508
82-83	22.294594581991205	26.618032440901914	27.11521049550981	23.97216248159707
84-85	22.362021306659877	26.670879890484255	26.77462626564035	24.192472537215515
86-87	22.350237485243664	26.570850265764463	26.888344630980303	24.190567618011574
88-89	22.314947880926216	26.546747721059695	26.894022694788816	24.24428170322528
90-91	22.21977196790053	26.574527410122634	26.90534182852886	24.300358793447973
92-93	22.217465985217896	26.511330238938243	26.916364095791884	24.35483968005198
94-95	21.99699165613977	26.521945704522036	26.986146908912396	24.4949157304258
96-97	22.120215775143386	26.458879518927546	26.945182718256667	24.475721987672397
98-99	22.08307770472862	26.471612327508034	26.7233479072346	24.721962060528742
100-101	22.039625970306844	26.566185417534633	26.79383997100172	24.600348641156803
102-103	21.85191736472978	26.47637114275383	26.876332276236255	24.795379216280136
104-105	21.832524416046148	26.60306329198076	26.651524629474572	24.91288766249852
106-107	21.725544242476012	26.71529208789933	26.72172882276631	24.837434846858347
108-109	21.674406027376822	26.727113690697486	26.722722137399067	24.87575814452662
110-111	21.548092674935642	26.910348171726937	26.529752490228294	25.011806663109127
112-113	21.434212091549025	26.99287652263257	26.44767115813542	25.125240227682983
114-115	21.479723115511458	27.004674647792214	26.401044565637342	25.11455767105898
116-117	21.415021272692	27.00670890625728	26.427917231256494	25.150352589794224
118-119	21.43618391500843	26.899590347904272	26.334249101738415	25.329976635348885
120-121	21.409676472945627	27.087366274767394	26.24233393379141	25.26062331849557
122-123	21.27412223420712	27.180555536758668	26.129358378006017	25.415963851028195
124-125	21.13716954315943	27.266593284062157	26.022675375270765	25.57356179750765
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	76.0
1	85.0
2	186.0
3	337.5
4	302.5
5	220.5
6	275.0
7	496.0
8	791.5
9	836.5
10	629.5
11	495.0
12	543.0
13	652.5
14	750.5
15	867.0
16	1039.5
17	1243.5
18	1501.0
19	1793.5
20	2198.5
21	2684.0
22	3238.0
23	4316.0
24	6097.0
25	8181.0
26	11554.5
27	16502.5
28	21378.5
29	27146.5
30	34396.0
31	42266.0
32	50321.0
33	57760.0
34	64736.5
35	72272.5
36	80578.5
37	87065.5
38	91077.0
39	94407.5
40	99289.5
41	105613.0
42	111450.5
43	116735.0
44	120594.5
45	124355.5
46	129319.0
47	134188.0
48	138409.0
49	140858.5
50	143785.0
51	140446.5
52	128128.0
53	118497.5
54	112228.5
55	104489.0
56	94691.5
57	84325.0
58	75935.5
59	67215.0
60	55833.0
61	44680.5
62	35258.5
63	26338.0
64	18850.5
65	14308.0
66	10924.0
67	8371.5
68	6580.0
69	5094.5
70	4076.5
71	3134.0
72	2510.0
73	2166.0
74	1598.5
75	1145.0
76	813.0
77	521.0
78	345.0
79	219.0
80	148.5
81	132.0
82	85.0
83	53.0
84	40.5
85	17.5
86	9.5
87	6.0
88	4.0
89	4.0
90	5.5
91	6.5
92	3.5
93	3.5
94	6.0
95	7.0
96	7.0
97	5.5
98	3.5
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.052298707118217935
2	2.4059209715108885E-4
3	0.002586365044374205
4	0.12688225723505547
5	0.017894037225612232
6	0.0021653288743597996
7	0.004300583736575713
8	0.011277754553957289
9	0.027096684941641382
10-11	0.020480402269986438
12-13	0.018961664656720188
14-15	0.028540237524547915
16-17	0.03804362536201592
18-19	0.0417728028678578
20-21	0.021593140719310223
22-23	0.023217137375080073
24-25	0.021457807664662733
26-27	0.020375143227482836
28-29	0.02010447711818786
30-31	0.02463061594584272
32-33	0.02389380264831751
34-35	0.028344756445612653
36-37	0.03058527035033217
38-39	0.031021343526418516
40-41	0.03317163539470638
42-43	0.03142734269036098
44-45	0.028359793451684597
46-47	0.04001347315744046
48-49	0.0320288229332387
50-51	0.022104398925756285
52-53	0.02995371609531056
54-55	0.031307046641785435
56-57	0.024525356903339117
58-59	0.02826957141525294
60-61	0.042404357122879405
62-63	0.05055441441387254
64-65	0.04365242862685068
66-67	0.03844962452595838
68-69	0.02828460842132488
70-71	0.035652741396576974
72-73	0.03878043865954113
74-75	0.0229464712657851
76-77	0.019788699990677058
78-79	0.03139726867821709
80-81	0.023562988514734762
82-83	0.03820903242880729
84-85	0.02034506921533895
86-87	0.034103929771166844
88-89	0.033291931443281915
90-91	0.03644970271838996
92-93	0.02526217020086433
94-95	0.02133751161608719
96-97	0.016781298776288447
98-99	0.024314838818331914
100-101	0.023908839654389454
102-103	0.02802897931810185
104-105	0.02497646708549741
106-107	0.013924267622619267
108-109	0.017021890873439534
110-111	0.022134472937900173
112-113	0.012616048094360222
114-115	0.014871599005151679
116-117	0.013382935404029317
118-119	0.007082429859885178
120-121	0.005067471046244809
122-123	0.003353252354043301
124-125	0.07104985368993093
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	3325130.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.693262577323
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.99996083751616	29.497535798041447
2	14.585712939889827	13.037661965999076
3	6.715861914050134	9.004613398730568
4	3.8273171998118887	6.842211703115894
5	2.3537164623160223	5.2597633941428885
6	1.5363595822931253	4.119895333476776
7	1.085802106450422	3.3969627055419385
8	0.7648941303553932	2.734849136946137
9	0.5605909397173903	2.2549174260531815
>10	2.458558331993438	18.102544153687035
>50	0.08071404391658875	2.4667249718949362
>100	0.02868555685582462	2.222747494887226
>500	0.0010820473103192868	0.318724104797976
>1k	7.439075233823157E-4	0.7408484126849497
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4207	0.12652136908932884	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3433	0.10324408368996099	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	3.0074012143886103E-5	0.0
12-13	0.0	0.0	0.0	6.0148024287772207E-5	0.0
14-15	0.0	0.0	0.0	6.0148024287772207E-5	0.0
16-17	0.0	0.0	0.0	7.518503035971526E-5	0.0
18-19	0.0	0.0	0.0	1.0525904250360136E-4	0.0
20-21	0.0	0.0	0.0	2.7066610929497495E-4	0.0
22-23	0.0	0.0	0.0	7.217762914532665E-4	0.0
24-25	0.0	0.0	0.0	0.001458589588978476	0.0
26-27	0.0	3.0074012143886103E-5	0.0	0.002601402050446148	0.0
28-29	0.0	3.0074012143886103E-5	0.0	0.006285468538072195	0.0
30-31	0.0	3.0074012143886103E-5	0.0	0.02129240059787136	0.0
32-33	0.0	3.0074012143886103E-5	0.0	0.0548700351565202	0.0
34-35	0.0	3.0074012143886103E-5	0.0	0.10577030071004742	0.0
36-37	0.0	3.0074012143886103E-5	0.0	0.1893760544700508	0.0
38-39	0.0	3.0074012143886103E-5	0.0	0.31248402318104856	0.0
40-41	0.0	3.0074012143886103E-5	0.0	0.44598256308775897	0.0
42-43	0.0	3.0074012143886103E-5	0.0	0.5713611197156201	0.0
44-45	0.0	3.0074012143886103E-5	0.0	0.7196861476092664	0.0
46-47	0.0	3.0074012143886103E-5	0.0	0.8978596325557197	0.0
48-49	0.0	3.0074012143886103E-5	0.0	1.0775067440972235	0.0
50-51	0.0	3.0074012143886103E-5	0.0	1.2601913308652595	0.0
52-53	0.0	3.0074012143886103E-5	0.0	1.4449961354894394	0.0
54-55	0.0	3.0074012143886103E-5	0.0	1.6575893273345703	0.0
56-57	0.0	3.0074012143886103E-5	0.0	1.9038804497869255	0.0
58-59	0.0	3.0074012143886103E-5	0.0	2.1677498323373823	0.0
60-61	0.0	3.0074012143886103E-5	0.0	2.432461287227868	0.0
62-63	0.0	3.0074012143886103E-5	0.0	2.6990674048834182	0.0
64-65	0.0	3.0074012143886103E-5	0.0	2.990018435369444	0.0
66-67	0.0	3.0074012143886103E-5	0.0	3.3286518121096016	0.0
68-69	0.0	3.0074012143886103E-5	0.0	3.7028176341977606	0.0
70-71	0.0	3.0074012143886103E-5	0.0	4.102471181577863	0.0
72-73	0.0	3.0074012143886103E-5	0.0	4.500094733138253	0.0
74-75	0.0	3.0074012143886103E-5	0.0	4.908665225119018	0.0
76-77	0.0	3.0074012143886103E-5	0.0	5.3657751727000145	0.0
78-79	0.0	3.0074012143886103E-5	0.0	5.874702643204928	0.0
80-81	0.0	3.0074012143886103E-5	0.0	6.409764430262877	0.0
82-83	0.0	3.0074012143886103E-5	0.0	6.953728124915417	0.0
84-85	0.0	3.0074012143886103E-5	0.0	7.506247876022892	0.0
86-87	0.0	3.0074012143886103E-5	0.0	8.09926228448211	0.0
88-89	0.0	3.0074012143886103E-5	0.0	8.761131143744755	0.0
90-91	0.0	3.0074012143886103E-5	0.0	9.456923488705705	0.0
92-93	0.0	3.0074012143886103E-5	0.0	10.15725700950038	0.0
94-95	0.0	3.0074012143886103E-5	0.0	10.87402597793169	0.0
96-97	0.0	3.0074012143886103E-5	0.0	11.629800940113618	0.0
98-99	0.0	3.0074012143886103E-5	0.0	12.448626068755207	0.0
100-101	0.0	3.0074012143886103E-5	0.0	13.320862642964336	0.0
102-103	0.0	3.0074012143886103E-5	0.0	14.197655430013263	0.0
104-105	0.0	3.0074012143886103E-5	0.0	15.070102522307398	0.0
106-107	1.5037006071943052E-5	3.0074012143886103E-5	0.0	15.956970103424528	0.0
108-109	3.0074012143886103E-5	3.0074012143886103E-5	0.0	16.89152003079579	0.0
110-111	3.0074012143886103E-5	3.0074012143886103E-5	0.0	17.870624607158216	0.0
112-113	3.0074012143886103E-5	3.0074012143886103E-5	0.0	18.867262332600532	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	11550	0.0	33.742165	1
TCAACGC	13380	0.0	28.229235	4
CAACGCA	13435	0.0	28.080256	5
ATCAACG	13660	0.0	27.780182	3
TATCAAC	13800	0.0	27.709188	2
AACGCAG	13785	0.0	27.62131	6
GGTATCA	8235	0.0	27.208303	1
CATGGGG	6915	0.0	25.243027	4
ACGCAGA	15655	0.0	24.208645	7
CGCAGAG	15935	0.0	23.672325	8
TACATGG	13310	0.0	23.135576	2
GTACATG	13585	0.0	22.897497	1
TACCGTA	275	5.527545E-8	21.634727	7
ACATGGG	14220	0.0	21.533264	3
GAGTACT	8375	0.0	21.208134	12-13
GCAGAGT	17475	0.0	20.905542	9
AGTACTT	9470	0.0	20.326717	12-13
AGAGTAC	15525	0.0	19.373423	10-11
CCCTATA	840	0.0	19.144865	2
GTATATA	1835	0.0	18.510406	1
>>END_MODULE