FastQCFastQC Report
Mon 15 Aug 2016
SRR2920955_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920955_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3276589
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA80000.033.404361
TCAACGC89750.029.2378144
CAACGCA91000.028.8998175
ATCAACG92600.028.210723
AACGCAG94550.028.0018166
TATCAAC95450.027.4930742
GGTATCA55800.026.1323621
GTACATG128250.026.0346831
CATGGGG80300.025.7131144
TACATGG131600.025.0956082
ACGCAGA104000.024.8281357
ACATGGG133600.024.0072783
CGCAGAG108150.023.7103798
GTACCGT4201.8189894E-1221.248576
GCAGAGT120450.019.80739
AGAGTAC103200.018.79470810-11
ATGGGGG43950.017.5994345
ATGGGGA48200.016.7882775
CAGAGTA112600.016.45952610-11
GTATTAA20500.016.258521
Produced by FastQC (version 0.11.5)