FastQCFastQC Report
Mon 15 Aug 2016
SRR2920955_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920955_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3276589
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA97050.036.7222371
GGTATCA67250.033.7641751
TCAACGC118850.028.969854
TATCAAC122700.028.7361372
ATCAACG121100.028.6274853
CAACGCA119750.028.4668545
AACGCAG124600.027.4027776
ACGCAGA142700.023.8041467
CGCAGAG149500.022.6425238
GTACATG136400.022.5454461
CATGGGG82850.022.4326044
TACATGG136900.021.883452
ACATGGG138000.021.2368643
GCAGAGT161850.019.9229479
AGAGTAC141800.019.76187910-11
GAGTACT66200.019.27950912-13
AGTACTT72100.019.22858412-13
TATACCG3303.776604E-718.0279525
CAGAGTA151850.017.59199510-11
TATACTG15650.017.4865385