FastQCFastQC Report
Mon 15 Aug 2016
SRR2920954_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920954_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2446570
Sequences flagged as poor quality0
Sequence length125
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA60000.028.6649131
GTACATG93500.026.4780371
TACATGG95450.025.5577832
CATGGGG61850.025.0120284
TCAACGC70550.024.542074
ATCAACG71650.024.2483353
GTATAGG6400.024.1767221
AACGCAG71650.023.7486256
CAACGCA71950.023.732785
ACATGGG103000.023.395543
TATCAAC73800.023.2194212
GGTATCA44650.021.8588051
TATAGGG4800.021.0728342
ACGCAGA79100.021.0605727
CGCAGAG81800.020.3654178
GTATAAG12350.018.7932421
ATGGGGA38100.017.646195
GCAGAGT88700.017.64099
GTATAAT13200.017.583071
GTGTAGC15050.017.3988361
Produced by FastQC (version 0.11.5)