##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920954_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2446570
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.73670199503795	31.0	28.0	31.0	12.0	34.0
2	27.868479953567647	31.0	28.0	33.0	15.0	34.0
3	27.959899369321132	31.0	28.0	34.0	15.0	34.0
4	31.37187327564713	35.0	32.0	37.0	16.0	37.0
5	31.423595891390804	35.0	32.0	37.0	16.0	37.0
6	31.35656980997887	35.0	32.0	37.0	16.0	37.0
7	31.210514311873357	35.0	32.0	37.0	13.0	37.0
8	31.133501596112108	35.0	32.0	37.0	11.0	37.0
9	32.29897652632052	37.0	32.0	39.0	10.0	39.0
10-11	32.22095402951888	37.0	32.0	39.0	10.0	39.0
12-13	32.128856317211444	37.0	32.0	39.0	10.0	39.0
14-15	32.96873868313598	38.0	32.0	40.0	10.0	41.0
16-17	32.846125391875155	38.0	32.0	40.0	10.0	41.0
18-19	32.902480411351405	38.0	32.0	40.0	10.0	41.0
20-21	32.919421680148126	38.0	32.0	40.0	8.5	41.0
22-23	32.84988759773887	38.0	31.5	40.0	8.0	41.0
24-25	32.76474635918858	38.0	31.5	40.0	8.0	41.0
26-27	32.61312143122821	38.0	31.0	40.0	7.5	41.0
28-29	32.52965110338147	38.0	31.0	40.0	2.0	41.0
30-31	32.38949038858483	38.0	31.0	40.0	2.0	41.0
32-33	32.29920644003646	38.0	30.0	40.0	2.0	41.0
34-35	32.12251744278725	38.0	30.0	40.0	2.0	41.0
36-37	31.961206914169637	38.0	30.0	40.0	2.0	41.0
38-39	31.81839820646865	38.0	30.0	40.0	2.0	41.0
40-41	31.731479172882853	38.0	30.0	40.0	2.0	41.0
42-43	31.509104991886602	37.0	30.0	40.0	2.0	41.0
44-45	31.339156860420914	37.0	30.0	40.0	2.0	41.0
46-47	31.131456896798376	37.0	29.5	40.0	2.0	41.0
48-49	30.955379163481936	37.0	29.0	40.0	2.0	41.0
50-51	30.710886874277048	36.5	28.5	40.0	2.0	41.0
52-53	30.523598139436025	36.0	28.0	40.0	2.0	41.0
54-55	30.3129544627785	36.0	28.0	40.0	2.0	41.0
56-57	30.100146940410454	35.5	28.0	39.0	2.0	41.0
58-59	29.837535406712256	35.0	28.0	39.0	2.0	41.0
60-61	29.650976877833045	35.0	27.5	39.0	2.0	40.5
62-63	29.53849266524154	35.0	28.0	39.0	2.0	41.0
64-65	29.30504359164054	35.0	27.0	39.0	2.0	40.0
66-67	29.04074459345124	34.5	26.5	38.0	2.0	40.0
68-69	28.739431530673556	34.0	26.0	37.5	2.0	40.0
70-71	28.41605329093384	34.0	26.0	37.0	2.0	39.5
72-73	28.12943937839506	34.0	26.0	36.5	2.0	39.0
74-75	27.834640128833428	34.0	26.0	36.0	2.0	39.0
76-77	27.560571943578154	34.0	26.0	36.0	2.0	38.5
78-79	27.25129364784167	34.0	25.5	35.0	2.0	37.0
80-81	26.94985265085405	33.0	25.0	35.0	2.0	37.0
82-83	26.691635228094842	33.0	24.5	35.0	2.0	36.5
84-85	26.450313704492412	33.0	24.0	35.0	2.0	36.0
86-87	26.377731068393707	33.0	24.0	35.0	2.0	36.0
88-89	26.480212297216102	33.0	25.0	35.0	2.0	35.0
90-91	26.397553718062433	33.0	25.0	35.0	2.0	35.0
92-93	26.251167961676963	33.0	24.0	35.0	2.0	35.0
94-95	26.167285832819008	33.0	24.0	35.0	2.0	35.0
96-97	26.075206717976595	33.0	24.0	35.0	2.0	35.0
98-99	26.013143094209447	33.0	23.5	35.0	2.0	35.0
100-101	25.85184891501163	33.0	23.0	35.0	2.0	35.0
102-103	24.57630826013562	31.0	19.5	34.0	2.0	35.0
104-105	25.55875368372865	32.5	20.5	35.0	2.0	35.0
106-107	25.730653322815208	33.0	23.0	35.0	2.0	35.0
108-109	25.73044139346105	33.0	22.0	35.0	2.0	35.0
110-111	25.63839456872274	33.0	20.0	35.0	2.0	35.0
112-113	25.55391813845506	33.0	19.5	35.0	2.0	35.0
114-115	25.437881810044267	33.0	19.0	35.0	2.0	35.0
116-117	25.30105433320935	33.0	18.5	35.0	2.0	35.0
118-119	25.11172355583531	33.0	16.5	35.0	2.0	35.0
120-121	24.90633172155303	33.0	6.0	35.0	2.0	35.0
122-123	24.678666663941762	33.0	2.0	35.0	2.0	35.0
124-125	23.550992409781855	31.5	2.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	180296.0
3	24965.0
4	15622.0
5	13520.0
6	14532.0
7	16021.0
8	17385.0
9	18538.0
10	18918.0
11	19276.0
12	19489.0
13	19498.0
14	19260.0
15	17297.0
16	15160.0
17	13525.0
18	12411.0
19	11847.0
20	12087.0
21	13120.0
22	15591.0
23	20672.0
24	28044.0
25	35882.0
26	41913.0
27	47421.0
28	55387.0
29	64244.0
30	73421.0
31	87158.0
32	105971.0
33	130496.0
34	167126.0
35	227846.0
36	343794.0
37	405244.0
38	103501.0
39	92.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.4410963922565	18.601526637656	12.984720130740174	24.97265683934733
2	16.017256378139198	19.481981613691293	39.424847353084495	25.075914655085015
3	19.883583403889464	23.554655416932572	27.84971482512777	28.712046354050198
4	12.94975440346513	15.51916634131684	35.41626721884153	36.1148120363765
5	14.648625447112929	36.95558507920286	33.71070005109862	14.68508942258559
6	34.51995654139481	34.766720649295756	16.646951153214474	14.066371656094956
7	30.0608060273014	31.085548635829284	20.028915154418534	18.824730182450782
8	26.547132676189328	35.2470625744703	19.28917265665077	18.916632092689596
9	26.419587468317022	16.410239431547804	18.70972567783485	38.46044742230032
10-11	25.763436122177573	25.79059786699574	26.873470619024136	21.572495391802548
12-13	26.329664768719784	22.950373720244986	27.097175438922783	23.622786072112444
14-15	23.363626089279258	24.489023057087948	24.39080168950562	27.75654916412717
16-17	24.086063829917684	27.72672594238459	24.824741787664422	23.362468440033304
18-19	23.890154720785457	26.63377297631356	26.449330971113216	23.02674133178777
20-21	24.51332316911167	25.992421796124244	26.133930651668308	23.360324383095783
22-23	24.224284266764545	25.70997001079481	25.92580481797407	24.13994090446658
24-25	24.05642851566444	25.808563887859087	26.189368693361086	23.945638903115395
26-27	23.870956534534486	26.060599867568072	25.969757289886942	24.098686308010496
28-29	23.937054123325243	26.154208364593796	26.219791462507168	23.688946049573794
30-31	23.87634335534067	25.953160238010327	26.0056631170851	24.1648332895639
32-33	23.779147949987127	25.99582272324111	26.030994412585784	24.194034914185984
34-35	24.227274724623406	25.977238191686027	25.859372205741238	23.936114877949333
36-37	24.106101058145153	25.877619942908026	25.861657735528908	24.154621263417912
38-39	24.122352383365897	26.029821707390845	25.991318351631282	23.856507557611977
40-41	24.130197402747097	25.896176508396636	25.984566408200294	23.989059680655977
42-43	24.18072304422982	25.798165310112292	25.795120164707626	24.225991480950267
44-45	24.224360429838725	25.984638340059483	25.67939161564745	24.11160961445434
46-47	23.98395147504284	26.20452343881428	25.61153420276471	24.19999088337817
48-49	24.272192689217025	26.103924795714057	25.654202446383305	23.969680068685612
50-51	24.037400491307086	26.27219573471543	25.605135689909563	24.08526808406792
52-53	24.397914768577163	26.09353458089162	25.36327849259709	24.145272157934127
54-55	24.057290142420147	25.974775235800085	25.639876986663744	24.328057635116025
56-57	24.133805614731518	26.263915434053636	25.564158550522283	24.038120400692563
58-59	24.18852788035405	25.90822582240647	25.70409063373471	24.199155663504765
60-61	24.24701140503509	25.86208094578858	25.81822328657772	24.07268436259861
62-63	24.060615239406815	26.059471902715416	25.758329199621393	24.121583658256377
64-65	23.942296313389203	25.705900543488593	25.792397618387252	24.559405524734952
66-67	23.997616252644523	26.251957844621526	25.523018366953217	24.227407535780735
68-69	24.074093754534548	25.89236846999483	25.817506269172835	24.216031506297785
70-71	23.858670693715585	25.82903068172273	25.774870716074457	24.537427908487235
72-73	24.180658326617433	25.84647439192563	25.751739815317652	24.221127466139286
74-75	24.073174465208265	25.916331107261225	25.576626385913308	24.433868041617202
76-77	24.06502882022247	25.75541191541054	25.864034438755702	24.31552482561129
78-79	24.08923707824226	25.924036653074122	25.779956890517568	24.20676937816605
80-81	23.96192245884549	26.03326450659856	25.733507488202957	24.271305546353
82-83	24.146341214182872	26.03948224892885	25.546970878290487	24.267205658597796
84-85	24.106381943828495	25.861961142722613	25.481505945327754	24.55015096812114
86-87	23.995271094483268	25.807650296455936	25.939976257362346	24.25710235169845
88-89	23.984844087845435	25.675496715810297	25.842056430022442	24.49760276632183
90-91	23.84297701383862	25.99056470741151	25.973704292436846	24.192753986313022
92-93	23.912118421359207	25.823389060502794	25.880984293955606	24.383508224182396
94-95	23.779073225256145	25.871284714607672	25.924300731208184	24.425341328928006
96-97	24.096880970346614	25.706692837750456	25.896517670795344	24.29990852110759
98-99	23.781559249375345	25.68021323096329	25.789571351319758	24.74865616834161
100-101	23.613136373577632	25.77938708642233	26.063584724785738	24.5438918152143
102-103	23.86040899213703	25.66035699745422	25.898236758932704	24.580997251476045
104-105	23.830611974198202	25.742795028732818	25.881528446584262	24.545064550484717
106-107	23.662026472490375	25.849718865716987	25.907523704480734	24.580730957311903
108-109	23.704450247226873	25.835678336908746	25.856893738227292	24.602977677637085
110-111	23.42892452550191	25.9161992239927	26.093419309262742	24.56145694124265
112-113	23.667403021568884	25.929318513041327	25.852719618411612	24.550558846978177
114-115	23.50731305107465	26.11431080232013	25.765735456371257	24.612640690233963
116-117	23.483054143477485	25.879012210941266	25.876028299740828	24.761905345840418
118-119	23.62692779878646	25.91552623355517	25.6211231218134	24.83642284584497
120-121	23.508598528372428	25.91038612953609	25.873188721255293	24.70782662083619
122-123	23.475673936778353	25.90239408046651	25.843552733823334	24.7783792489318
124-125	23.467631533477764	25.943173325345033	25.68279015271491	24.90640498846229
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	526.0
1	416.0
2	269.0
3	204.0
4	162.0
5	151.0
6	156.5
7	189.0
8	212.5
9	232.5
10	273.0
11	318.5
12	352.0
13	380.5
14	434.5
15	505.0
16	594.0
17	689.5
18	780.5
19	911.0
20	1089.5
21	1362.5
22	1733.0
23	2260.0
24	2978.0
25	3925.5
26	5259.0
27	7011.0
28	8931.5
29	11183.5
30	14223.0
31	18112.0
32	22530.0
33	27404.5
34	32019.5
35	35710.5
36	40239.5
37	45570.0
38	50263.5
39	53488.0
40	57278.5
41	63324.0
42	70021.0
43	76479.0
44	81575.0
45	86123.0
46	90367.0
47	94545.0
48	101391.5
49	106773.0
50	110175.5
51	110905.5
52	106814.0
53	104473.5
54	103168.0
55	98216.0
56	90027.5
57	80951.0
58	73622.0
59	66337.5
60	57326.0
61	47571.0
62	39070.5
63	32034.5
64	24830.5
65	18453.0
66	13957.5
67	10957.0
68	8375.5
69	6303.0
70	4996.0
71	4221.5
72	3547.5
73	2936.5
74	2155.5
75	1400.5
76	953.5
77	659.5
78	435.0
79	284.0
80	198.0
81	128.0
82	94.5
83	77.5
84	57.5
85	35.5
86	26.5
87	25.0
88	16.5
89	10.0
90	9.0
91	11.5
92	8.0
93	5.5
94	6.5
95	5.0
96	4.5
97	3.5
98	4.5
99	5.0
100	8.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.032821460248429433
2	0.016431166898964674
3	0.02154036058645369
4	0.01982367150745738
5	0.01307953583997188
6	0.00425084914799086
7	0.004169102048991036
8	0.005109193687489015
9	0.0027385278164941125
10-11	0.004680021417739938
12-13	0.0063149633977364235
14-15	0.0022684819972451226
16-17	0.0015123213314967485
18-19	6.131032424986819E-5
20-21	0.00326988395999297
22-23	9.400916384979789E-4
24-25	0.0016758155294963968
26-27	0.0012262064849973635
28-29	0.0022684819972451226
30-31	0.0015327581062467047
32-33	0.0
34-35	0.0060288485512370385
36-37	0.0068054459917353686
38-39	0.0012262064849973635
40-41	9.605284132479349E-4
42-43	0.0021662981234953425
44-45	0.001001401962747847
46-47	0.01996672893070707
48-49	0.002472849744744683
50-51	0.009748341555729042
52-53	0.0012670800344972758
54-55	2.4524129699947275E-4
56-57	2.4524129699947275E-4
58-59	0.006212779523986643
60-61	7.765974404983304E-4
62-63	0.009053491214230536
64-65	0.009993582852728514
66-67	8.174709899982424E-5
68-69	0.0030655162124934095
70-71	0.0046391478682400265
72-73	0.010688433194227019
74-75	0.0013692639082470562
76-77	9.80965187997891E-4
78-79	3.47425170749253E-4
80-81	0.003617309130742223
82-83	0.0011240226112475833
84-85	0.0011035858364976273
86-87	0.013815259730970298
88-89	0.0
90-91	4.496090444990334E-4
92-93	0.007643353756483567
94-95	0.0057018601552377415
96-97	0.00425084914799086
98-99	8.583445394981546E-4
100-101	0.015736316557466167
102-103	0.006785009216985413
104-105	0.006192342749236686
106-107	0.016676408195964144
108-109	0.009564410582979438
110-111	0.018760959220459665
112-113	0.002431976195244771
114-115	0.013365650686471265
116-117	0.004761768516739762
118-119	0.0038625504277416955
120-121	0.006662388568485675
122-123	0.006580641469485852
124-125	0.05425963696113334
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2446570.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.388754154791705
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.2646131830354	39.95408070756327
2	12.04049174436063	12.615727237962163
3	4.571188116640128	7.184365513138949
4	2.2354867327837864	4.684574594404333
5	1.279749069020889	3.3522229678379456
6	0.7621846363782956	2.3957942121469102
7	0.5005382973387064	1.8355804463034864
8	0.3852498841540391	1.614620917528635
9	0.27862230429954765	1.3137007861791508
>10	1.5158960212414327	14.381758653644127
>50	0.10648616744701414	3.8274686066163537
>100	0.05537958749666443	5.152379945023978
>500	0.003323052764450891	1.1499512757678434
>1k	7.912030391542957E-4	0.5377741358829953
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	8.174709899982425E-5	0.0	0.0	0.0	0.0
7	2.4524129699947275E-4	0.0	0.0	0.0	0.0
8	2.4524129699947275E-4	0.0	0.0	0.0	0.0
9	2.4524129699947275E-4	0.0	0.0	0.0	0.0
10-11	3.26988395999297E-4	0.0	0.0	0.0	0.0
12-13	3.47425170749253E-4	0.0	0.0	4.0873549499912125E-5	0.0
14-15	4.496090444990333E-4	0.0	0.0	8.174709899982425E-5	0.0
16-17	4.904825939989455E-4	0.0	0.0	1.0218387374978031E-4	0.0
18-19	6.131032424986819E-4	0.0	0.0	1.634941979996485E-4	0.0
20-21	7.357238909984181E-4	0.0	0.0	2.0436774749956063E-4	0.0
22-23	8.583445394981545E-4	0.0	0.0	3.065516212493409E-4	0.0
24-25	0.0010627122869977153	0.0	0.0	5.517929182488137E-4	0.0
26-27	0.0011035858364976273	0.0	0.0	7.970342152482863E-4	0.0
28-29	0.001369263908247056	0.0	0.0	0.0018801832769959575	0.0
30-31	0.0015940684304965728	0.0	0.0	0.006151469199736774	0.0
32-33	0.0015940684304965728	0.0	0.0	0.015123213314967486	0.0
34-35	0.001737125853746265	0.0	0.0	0.02754877236294077	0.0
36-37	0.001961930375995782	0.0	0.0	0.047495064518897886	0.0
38-39	0.0021662981234953425	0.0	0.0	0.08315723645757121	0.0
40-41	0.0022071716729952545	0.0	0.0	0.12611533698197885	0.0
42-43	0.002350229096244947	0.0	0.0	0.16410730124214717	0.0
44-45	0.0025750336184944636	4.0873549499912125E-5	0.0	0.20947694118704963	0.0
46-47	0.0032494471852430136	4.0873549499912125E-5	0.0	0.26512627883118	0.0
48-49	0.003433378157992618	4.0873549499912125E-5	0.0	0.3227171100765561	0.0
50-51	0.003433378157992618	4.0873549499912125E-5	0.0	0.3859689279276701	0.0
52-53	0.003433378157992618	4.0873549499912125E-5	0.0	0.44987472257078276	0.0
54-55	0.0034742517074925305	4.0873549499912125E-5	0.0	0.5210151354753798	0.0
56-57	0.0035151252569924425	4.0873549499912125E-5	0.0	0.6004324421537091	0.0
58-59	0.0035151252569924425	4.0873549499912125E-5	0.0	0.6898842052342667	0.0
60-61	0.0035151252569924425	4.0873549499912125E-5	0.0	0.7873267472420573	0.0
62-63	0.0035559988064923546	4.0873549499912125E-5	0.0	0.8889179545240888	0.0
64-65	0.003637745905492179	4.0873549499912125E-5	0.0	0.9949439419268609	0.0
66-67	0.003964734301491476	4.0873549499912125E-5	0.0	1.117748521399347	0.0
68-69	0.003964734301491476	4.0873549499912125E-5	0.0	1.2591710026690428	0.0
70-71	0.0040669181752412565	4.0873549499912125E-5	0.0	1.4147152952909585	0.0
72-73	0.004271285922740817	4.0873549499912125E-5	0.0	1.5735907821971167	0.0
74-75	0.004373469796490597	4.0873549499912125E-5	0.0	1.7392512783202605	0.0
76-77	0.004393906571240553	4.0873549499912125E-5	0.0	1.9240405956093634	0.0
78-79	0.0044347801207404655	4.0873549499912125E-5	0.0	2.1291031934504225	0.0
80-81	0.005047883363239147	4.0873549499912125E-5	0.0	2.3536011640786896	0.0
82-83	0.005190940786488839	4.0873549499912125E-5	0.0	2.5921391989601767	0.0
84-85	0.0053953085339884	4.0873549499912125E-5	0.0	2.838157093400148	0.0
86-87	0.0056609866057378295	4.0873549499912125E-5	0.0	3.1007287753875836	0.0
88-89	0.005722296929987697	4.0873549499912125E-5	0.0	3.3909718503864594	0.0
90-91	0.006417147271486203	4.0873549499912125E-5	0.0	3.7127693055992674	0.0
92-93	0.006621515018985764	4.0873549499912125E-5	0.0	4.0393489661035655	0.0
94-95	0.006621515018985764	4.0873549499912125E-5	0.0	4.3787833579255855	0.0
96-97	0.006621515018985764	4.0873549499912125E-5	0.0	4.726004160927339	0.0
98-99	0.007009813739234929	4.0873549499912125E-5	0.0	5.098157829124039	0.0
100-101	0.007357238909984182	4.0873549499912125E-5	0.0	5.514434494005894	0.0
102-103	0.007602480206983655	4.0873549499912125E-5	0.0	5.959670068708437	0.0
104-105	0.008706066043481281	4.0873549499912125E-5	0.0	6.3997147026244905	0.0
106-107	0.008992180889980668	4.0873549499912125E-5	0.0	6.843397082446037	0.0
108-109	0.009053491214230535	4.0873549499912125E-5	0.0	7.3023866065553005	0.0
110-111	0.00923742218698014	4.0873549499912125E-5	0.0	7.8054991273497185	0.0
112-113	0.00933960606072992	4.0873549499912125E-5	0.0	8.33943030446707	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6000	0.0	28.664913	1
GTACATG	9350	0.0	26.478037	1
TACATGG	9545	0.0	25.557783	2
CATGGGG	6185	0.0	25.012028	4
TCAACGC	7055	0.0	24.54207	4
ATCAACG	7165	0.0	24.248335	3
GTATAGG	640	0.0	24.176722	1
AACGCAG	7165	0.0	23.748625	6
CAACGCA	7195	0.0	23.73278	5
ACATGGG	10300	0.0	23.39554	3
TATCAAC	7380	0.0	23.219421	2
GGTATCA	4465	0.0	21.858805	1
TATAGGG	480	0.0	21.072834	2
ACGCAGA	7910	0.0	21.060572	7
CGCAGAG	8180	0.0	20.365417	8
GTATAAG	1235	0.0	18.793242	1
ATGGGGA	3810	0.0	17.64619	5
GCAGAGT	8870	0.0	17.6409	9
GTATAAT	1320	0.0	17.58307	1
GTGTAGC	1505	0.0	17.398836	1
>>END_MODULE