FastQCFastQC Report
Mon 15 Aug 2016
SRR2920954_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920954_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2446570
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28180.11518166249075237No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA76550.035.810331
TACCGTA1606.9921953E-933.466277
GGTATCA55350.031.8690971
ATCAACG97450.027.5684643
TCAACGC97350.027.352584
CAACGCA98150.027.0952515
TATCAAC100450.026.8016742
AACGCAG101550.026.4202566
GTACATG103300.024.7487141
TACATGG105300.023.757152
ACGCAGA112400.023.3959527
ACATGGG107600.022.6980973
CGCAGAG115300.022.6015628
CATGGGG63850.022.4844824
AGTACTT55100.021.9221212-13
GAGTACT53550.021.88995412-13
GTACCGT3701.364242E-1020.901726
ACCGTGC6650.020.5778478
GTACTTT59100.020.38804814-15
GTATTAC4700.020.2869991
Produced by FastQC (version 0.11.5)