FastQCFastQC Report
Mon 15 Aug 2016
SRR2920953_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920953_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3464856
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA104150.027.827311
TCAACGC109850.025.4038244
CAACGCA111850.025.0016885
ATCAACG112500.024.7543163
AACGCAG113650.024.5537136
TATCAAC115750.024.4194852
GTACATG142500.024.3057841
TACATGG139650.023.947452
CATGGGG69800.023.442484
ACATGGG143400.022.698433
ACGCAGA125150.021.9171377
CGCAGAG125200.021.718298
GTATAGC19950.020.5829541
GTGTAGC27900.019.410591
TACACCG3503.4118784E-818.699595
GCAGAGT142700.018.345789
GACCGCG3905.9008016E-918.3075567
TAGCCCT35850.017.2611794
GGTATCA80650.017.1930561
GAGTACT41050.017.02896712-13
Produced by FastQC (version 0.11.5)