##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920953_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3464856
Sequences flagged as poor quality	0
Sequence length	125
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.556136243468703	31.0	29.0	33.0	18.0	34.0
2	28.65536028048496	31.0	28.0	34.0	16.0	34.0
3	28.736559037374136	31.0	28.0	34.0	16.0	34.0
4	32.19309027561319	35.0	33.0	37.0	20.0	37.0
5	32.27370372679269	35.0	33.0	37.0	22.0	37.0
6	32.21565946752188	35.0	33.0	37.0	22.0	37.0
7	32.10168445672778	35.0	32.0	37.0	19.0	37.0
8	32.053090518047505	35.0	32.0	37.0	19.0	37.0
9	33.2754867734763	37.0	33.0	39.0	18.0	39.0
10-11	33.22080499160715	37.0	33.0	39.0	17.5	39.0
12-13	33.153368711426964	37.0	33.0	39.0	17.0	39.0
14-15	34.064192999651354	38.0	33.0	41.0	17.0	41.0
16-17	33.934157869764284	38.0	33.0	41.0	17.0	41.0
18-19	34.05014176635335	38.5	33.0	41.0	17.5	41.0
20-21	34.07561122309268	39.0	33.0	41.0	18.0	41.0
22-23	34.02795729461773	39.0	33.0	41.0	18.0	41.0
24-25	33.96397079705477	39.0	33.0	41.0	18.0	41.0
26-27	33.82114639107657	38.0	32.5	41.0	17.0	41.0
28-29	33.74511812323514	38.0	32.5	41.0	16.0	41.0
30-31	33.62098280563464	38.0	32.0	40.0	16.0	41.0
32-33	33.524455273177296	38.0	32.0	40.0	15.5	41.0
34-35	33.3573406802476	38.0	32.0	40.0	13.0	41.0
36-37	33.21553752883237	38.0	32.0	40.0	9.0	41.0
38-39	33.09879386040863	38.0	32.0	40.0	9.0	41.0
40-41	33.027040373395025	38.0	32.0	40.0	9.0	41.0
42-43	32.83315439371795	38.0	31.0	40.0	8.0	41.0
44-45	32.68941104623107	38.0	31.0	40.0	7.5	41.0
46-47	32.51223196577289	38.0	31.0	40.0	4.0	41.0
48-49	32.37067745383935	38.0	31.0	40.0	2.0	41.0
50-51	32.164957360421326	37.5	30.5	40.0	2.0	41.0
52-53	32.00858736986472	37.0	30.0	40.0	2.0	41.0
54-55	31.820319228273846	37.0	30.0	40.0	2.0	41.0
56-57	31.647299050811924	37.0	30.0	40.0	2.0	41.0
58-59	31.418304829984276	36.5	30.0	40.0	2.0	41.0
60-61	31.263275299175493	36.0	29.5	40.0	2.0	41.0
62-63	31.170346184661064	36.0	30.0	40.0	2.0	41.0
64-65	30.966795734079568	36.0	29.0	39.0	2.0	41.0
66-67	30.72255989859319	35.0	29.0	39.0	2.0	41.0
68-69	30.418414935570194	35.0	29.0	39.0	2.0	40.5
70-71	30.0959542041574	35.0	29.0	38.5	2.0	40.0
72-73	29.80138164472059	35.0	29.0	37.5	2.0	40.0
74-75	29.481078145816163	34.5	29.0	37.0	2.0	39.0
76-77	29.168835443666346	34.0	28.5	36.5	2.0	39.0
78-79	28.822411234406278	34.0	28.0	36.0	2.0	39.0
80-81	28.480692271193952	34.0	28.0	36.0	2.0	37.5
82-83	28.17976634526803	34.0	27.0	35.0	2.0	37.0
84-85	27.904935443204565	34.0	26.5	35.0	2.0	36.5
86-87	27.79350859025599	34.0	26.5	35.0	2.0	36.0
88-89	27.844375494970066	34.0	27.0	35.0	2.0	36.0
90-91	27.73282857931181	34.0	27.0	35.0	2.0	36.0
92-93	27.561605446229223	34.0	27.0	35.0	2.0	35.0
94-95	27.468602302664237	34.0	27.0	35.0	2.0	35.0
96-97	27.36334583601743	34.0	27.0	35.0	2.0	35.0
98-99	27.294738944417894	34.0	26.5	35.0	2.0	35.0
100-101	27.119278261491964	34.0	26.0	35.0	2.0	35.0
102-103	25.822870416548334	31.5	24.0	34.0	2.0	35.0
104-105	26.806640882045315	33.5	25.0	35.0	2.0	35.0
106-107	26.98434639130746	34.0	25.0	35.0	2.0	35.0
108-109	26.97834340012976	34.0	25.0	35.0	2.0	35.0
110-111	26.875913457875306	34.0	25.0	35.0	2.0	35.0
112-113	26.78038423530444	34.0	25.0	35.0	2.0	35.0
114-115	26.656923548915167	34.0	25.0	35.0	2.0	35.0
116-117	26.524133181869608	34.0	25.0	35.0	2.0	35.0
118-119	26.338564142348197	34.0	25.0	35.0	2.0	35.0
120-121	26.131997837716778	34.0	24.0	35.0	2.0	35.0
122-123	25.91275351702928	34.0	23.5	35.0	2.0	35.0
124-125	24.798251211594362	32.5	11.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	190616.0
3	27257.0
4	17052.0
5	15112.0
6	17055.0
7	18828.0
8	19928.0
9	21382.0
10	22461.0
11	23636.0
12	24606.0
13	25113.0
14	25649.0
15	23500.0
16	19686.0
17	17695.0
18	16648.0
19	15961.0
20	15837.0
21	17116.0
22	20572.0
23	26873.0
24	36803.0
25	46696.0
26	54747.0
27	62432.0
28	73189.0
29	84868.0
30	99231.0
31	117736.0
32	142752.0
33	177191.0
34	227790.0
35	309128.0
36	476295.0
37	686935.0
38	246140.0
39	340.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.00325144665125	19.74244800846727	13.696119946103707	24.558180598777778
2	15.03567427511957	20.297315935491003	40.98675013227929	23.68025965711013
3	18.924126017656604	24.837143188708364	29.472218645841934	26.766512147793097
4	12.268659646205718	16.748158283682045	38.088829615260266	32.89435245485197
5	13.292937643387187	37.988786501312205	35.32119307377101	13.397082781529592
6	31.909111900020203	37.15808006465206	17.770485179091985	13.16232285623575
7	27.49788870974911	32.492049830490664	21.805082451440324	18.2049790083199
8	24.844003406973094	36.659769402991756	20.24355066400474	18.252676526030413
9	25.534364474409553	16.805531103074664	19.93321346005651	37.72689096245927
10-11	24.17763585135362	26.866536439166843	28.733072878333687	20.222754831145846
12-13	24.917757829115526	24.264496305578497	28.34629124068746	22.471454624618517
14-15	22.169530122020646	25.952653593695995	25.820682827854274	26.05713345642908
16-17	22.228528523458213	28.931866981202457	26.566729830695294	22.272874664644032
18-19	22.08861554091008	28.244573005219554	28.05531544610092	21.611496007769446
20-21	23.154962326025203	27.38163133246323	27.728440863721982	21.734965477789586
22-23	22.49254328602799	27.282820905669126	27.400561152991465	22.82407465531142
24-25	22.255504446488807	27.33209950033371	27.69771813049047	22.714677922687017
26-27	22.36219588414227	27.514067475684513	27.543664985626233	22.580071654546984
28-29	22.27429880606759	27.649346897296763	27.613716816330324	22.462637480305325
30-31	22.76525058459059	27.46989885183002	27.488312517425157	22.27653804615423
32-33	22.25435919992057	27.469164663697427	27.50863239338085	22.76784374300115
34-35	22.52261639255875	27.524286122079882	27.514833461437334	22.438264023924035
36-37	22.59840921967945	27.407291312187393	27.472421692731807	22.521877775401354
38-39	22.442411821461278	27.57326000189909	27.47355776654294	22.510770410096693
40-41	22.739891131319652	27.452002147877096	27.394827402836487	22.413279317966765
42-43	22.560362205703896	27.463759154102945	27.397896224902567	22.57798241529059
44-45	22.732364163217714	27.4553367851721	27.29101367459204	22.521285377018145
46-47	22.455770445966	27.471989464638153	27.276876272140036	22.79536381725581
48-49	22.50308679567231	27.51184931241951	27.40671931488433	22.578344577023852
50-51	22.54188519871341	27.643271416260852	27.184503851133474	22.63033953389227
52-53	22.580528783539258	27.584480669383225	27.096963563424918	22.7380269836526
54-55	22.580172674166658	27.5293198160555	27.10084521708663	22.78966229269121
56-57	22.594436668731692	27.516296888594116	27.09580231285726	22.793464129816932
58-59	22.545857108180087	27.487306692420326	27.234926994900743	22.731909204498844
60-61	22.74491230743655	27.44148922660608	27.172702745760834	22.640895720196536
62-63	22.468887000854522	27.441598137224947	27.30492651410727	22.784588347813262
64-65	22.48047952614278	27.376982011996816	27.28627637408939	22.856262087771018
66-67	22.617124394184167	27.50072333526463	27.067717889866884	22.814434380684315
68-69	22.735392215492816	27.32539177678594	27.11768427420565	22.82153173351559
70-71	22.480055878176337	27.212426544442774	27.302102935913275	23.00541464146761
72-73	22.664860698987425	27.21522093036681	27.23721582708894	22.882702543556825
74-75	22.691103104552834	27.28030215898558	27.114001001789862	22.91459373467173
76-77	22.626915830451026	27.182684448467104	27.26313605109189	22.927263669989976
78-79	22.677292071597524	27.2854407006614	27.16042773108892	22.876839496652153
80-81	22.695243946067066	27.253878787275298	27.1589213586532	22.891955908004434
82-83	22.66389922454667	27.276414997408228	27.187492423831365	22.872193354213735
84-85	22.756018174117397	27.183509665371986	27.125627828882763	22.934844331627858
86-87	22.61465856767878	27.07643365695513	27.204609420406257	23.104298354959838
88-89	22.683338066574773	27.120723054580047	27.218620340931913	22.977318537913263
90-91	22.643412105609496	27.18346301705129	27.218601724754965	22.954523152584247
92-93	22.52411314999936	27.168163036398795	27.278480345868722	23.029243467733124
94-95	22.456380623616376	27.24313996011118	27.287185067380932	23.01329434889151
96-97	22.56576697227317	27.14247574461821	27.28264681306724	23.009110470041374
98-99	22.568317090294077	27.086794614723576	27.17853104817209	23.166357246810257
100-101	22.476746627909243	27.190233475474358	27.203930395399333	23.129089501217063
102-103	22.424770280743125	27.24436471639215	27.217189509186607	23.11367549367812
104-105	22.37698687531921	27.226493142962948	27.137405852571717	23.25911412914612
106-107	22.18945864319813	27.379555959687806	27.23356318355182	23.19742221356225
108-109	22.333424930522764	27.381161306114343	27.06984150992194	23.215572253440957
110-111	22.234043213444814	27.46859174178914	27.050483715249495	23.24688132951655
112-113	22.24229742405657	27.558395266613754	27.007345407316546	23.191961902013134
114-115	22.2016924416282	27.57861228561598	26.946905388148522	23.272789884607302
116-117	22.26683149327306	27.428260451052644	27.07932198654352	23.22558606913077
118-119	22.251328583025163	27.35620456390367	27.015437900243526	23.377028952827636
120-121	22.2625107913233	27.540914665491346	26.97750007288022	23.219074470305134
122-123	22.268094828572803	27.44090748962382	27.029497222796696	23.261500459006683
124-125	22.254432949620114	27.51588633941575	26.86410279993168	23.365577911032457
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	974.0
1	707.0
2	367.5
3	268.0
4	213.0
5	189.0
6	196.5
7	221.0
8	274.5
9	327.0
10	356.0
11	403.5
12	497.5
13	613.5
14	679.0
15	754.0
16	931.0
17	1118.5
18	1328.5
19	1623.0
20	2046.5
21	2698.0
22	3502.5
23	4634.5
24	6088.0
25	8075.5
26	11119.0
27	15119.0
28	19440.5
29	24970.0
30	31861.0
31	39864.5
32	49926.0
33	60811.0
34	71261.0
35	80474.0
36	89402.5
37	98014.5
38	104843.0
39	110839.0
40	117551.0
41	123263.5
42	127539.0
43	133604.5
44	139086.0
45	141981.5
46	144012.0
47	145133.0
48	146095.5
49	146756.5
50	147572.0
51	141292.0
52	127600.5
53	117293.0
54	108771.5
55	99363.0
56	88557.0
57	77635.0
58	68795.0
59	59619.0
60	49176.5
61	39219.0
62	30606.5
63	23359.0
64	17792.0
65	13707.5
66	10219.0
67	7483.5
68	5765.5
69	4407.5
70	3364.0
71	2609.5
72	2147.0
73	1867.0
74	1449.0
75	1090.0
76	822.0
77	543.0
78	361.5
79	235.5
80	127.5
81	84.0
82	66.0
83	42.5
84	36.5
85	34.5
86	20.5
87	12.5
88	13.5
89	14.0
90	15.5
91	10.5
92	7.0
93	7.0
94	5.0
95	5.0
96	7.5
97	9.5
98	10.0
99	10.5
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03368105341174352
2	0.017172430831180283
3	0.02222314578152743
4	0.02008741488823778
5	0.01327616501234106
6	0.004502351612880882
7	0.004329184243154694
8	0.005685661972676498
9	0.0024820656327420245
10-11	0.004329184243154694
12-13	0.006941125403191359
14-15	0.0021213002791458002
16-17	0.0019481329094196123
18-19	2.886122828769796E-5
20-21	0.0037519596774007345
22-23	7.359613213362979E-4
24-25	0.0012554634305148612
26-27	0.0010822960607886735
28-29	0.0025109268610297225
30-31	0.0010967266749325225
32-33	0.0
34-35	0.006436053908156646
36-37	0.007705947952815355
38-39	0.0011255879032202206
40-41	0.0011400185173640694
42-43	0.0024387737903104776
44-45	0.0010390042183571267
46-47	0.020578055769128648
48-49	0.0028284003721944
50-51	0.010289027884564324
52-53	0.0015729369416795386
54-55	1.8759798387003672E-4
56-57	2.164592121577347E-4
58-59	0.006782388647609021
60-61	6.92669478904751E-4
62-63	0.01002927682997504
64-65	0.01017358297141353
66-67	1.0101429900694285E-4
68-69	0.003694237220825339
70-71	0.005079576178634841
72-73	0.01200627096768235
74-75	0.0016595206265426325
76-77	0.0011544491315079184
78-79	4.4734903845931835E-4
80-81	0.004199308715860053
82-83	0.0012698940446587103
84-85	0.0011111572890763713
86-87	0.013636930365937286
88-89	0.0
90-91	4.6177965260316736E-4
92-93	0.008788244013604028
94-95	0.006522637593019739
96-97	0.004646657754319371
98-99	0.0011111572890763713
100-101	0.01655191442299478
102-103	0.008687229714597086
104-105	0.007980129621548485
106-107	0.018572200403133635
108-109	0.01189082605453156
110-111	0.019611204621490762
112-113	0.0030592901984959836
114-115	0.014776948883301355
116-117	0.0059165517989780815
118-119	0.004863116966477106
120-121	0.007705947952815355
122-123	0.008932550155042518
124-125	0.054735319447619175
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	3464856.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.290399371753715
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.91564268911081	35.34710836485573
2	14.548366138189628	14.923830188684887
3	6.329714026208931	9.739606809397417
4	3.372623156552472	6.919327545200036
5	1.970387797677788	5.0530988530062
6	1.2802758791084061	3.9399516687295932
7	0.8523562348059146	3.0602384183139746
8	0.5789257526173499	2.375466644666958
9	0.4398457710028489	2.030387873104174
>10	1.6561705675176124	13.41869274031336
>50	0.04037104833149441	1.3829642799330952
>100	0.014358169485691994	1.2179476469633128
>500	5.097014459536682E-4	0.18645619216657106
>1k	4.530679451398861E-4	0.4049227746647222
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	2.886122828769796E-5	0.0	0.0	0.0
5	0.0	2.886122828769796E-5	0.0	0.0	0.0
6	0.0	2.886122828769796E-5	0.0	0.0	0.0
7	0.0	2.886122828769796E-5	0.0	0.0	0.0
8	0.0	2.886122828769796E-5	0.0	0.0	0.0
9	0.0	2.886122828769796E-5	0.0	0.0	0.0
10-11	0.0	5.772245657539592E-5	0.0	0.0	0.0
12-13	0.0	7.21530707192449E-5	0.0	4.329184243154694E-5	0.0
14-15	0.0	1.8759798387003672E-4	0.0	7.21530707192449E-5	0.0
16-17	0.0	2.597510545892816E-4	0.0	1.443061414384898E-4	0.0
18-19	0.0	2.741816687331306E-4	0.0	2.164592121577347E-4	0.0
20-21	0.0	2.886122828769796E-4	0.0	2.886122828769796E-4	0.0
22-23	0.0	2.886122828769796E-4	0.0	4.906408808908653E-4	0.0
24-25	0.0	3.4633473945237553E-4	0.0	7.936837779116939E-4	0.0
26-27	0.0	3.7519596774007345E-4	0.0	0.001356477729521804	0.0
28-29	0.0	3.8962658188392246E-4	0.0	0.0031891657257906245	0.0
30-31	0.0	4.184878101716204E-4	0.0	0.009076856296481008	0.0
32-33	0.0	4.6177965260316736E-4	0.0	0.02363734596762463	0.0
34-35	0.0	5.195021091785632E-4	0.0	0.04158902996257276	0.0
36-37	0.0	5.916551798978082E-4	0.0	0.07108520527260007	0.0
38-39	0.0	6.926694789047511E-4	0.0	0.12051005871528282	0.0
40-41	0.0	7.503919354801469E-4	0.0	0.1753896843043405	0.0
42-43	0.0	8.081143920555428E-4	0.0	0.2277439524182246	0.0
44-45	0.0	8.081143920555428E-4	0.0	0.28963685648119286	0.0
46-47	0.0	8.081143920555428E-4	0.0	0.36330514168554195	0.0
48-49	0.0	8.225450061993918E-4	0.0	0.44075424779557937	0.0
50-51	0.0	8.658368486309388E-4	0.0	0.5212337828758251	0.0
52-53	0.0	8.946980769186367E-4	0.0	0.6061290858840886	0.0
54-55	0.0	8.946980769186367E-4	0.0	0.6984561551764344	0.0
56-57	0.0	8.946980769186367E-4	0.0	0.8085761717081461	0.0
58-59	0.0	8.946980769186367E-4	0.0	0.9273978485685985	0.0
60-61	0.0	8.946980769186367E-4	0.0	1.0488747584315192	0.0
62-63	0.0	8.946980769186367E-4	0.0	1.1764125262348566	0.0
64-65	0.0	8.946980769186367E-4	0.0	1.316548220185774	0.0
66-67	0.0	9.524205334940326E-4	0.0	1.4767424677966416	0.0
68-69	0.0	9.524205334940326E-4	0.0	1.6621325677026693	0.0
70-71	0.0	9.668511476378816E-4	0.0	1.8641900269448428	0.0
72-73	0.0	9.812817617817307E-4	0.0	2.065684692235406	0.0
74-75	0.0	9.812817617817307E-4	0.0	2.280022604113995	0.0
76-77	0.0	0.0010967266749325225	0.0	2.516800120986269	0.0
78-79	0.0	0.0011111572890763716	0.0	2.7840983867727838	2.886122828769796E-5
80-81	0.0	0.0011255879032202203	0.0	3.0689442793582185	2.886122828769796E-5
82-83	0.0	0.0011255879032202203	0.0	3.3683362310006535	2.886122828769796E-5
84-85	0.0	0.0011255879032202203	0.0	3.675665020422205	2.886122828769796E-5
86-87	0.0	0.0011255879032202203	0.0	4.0093008194279935	2.886122828769796E-5
88-89	0.0	0.0011255879032202203	0.0	4.386488211919918	5.772245657539592E-5
90-91	0.0	0.0011255879032202203	0.0	4.784354674479978	5.772245657539592E-5
92-93	0.0	0.0011688797456517672	0.0	5.1965074450424495	5.772245657539592E-5
94-95	0.0	0.0012121715880833142	0.0	5.626092397490689	5.772245657539592E-5
96-97	0.0	0.0012121715880833142	0.0	6.059559185143625	5.772245657539592E-5
98-99	0.0	0.0012121715880833142	0.0	6.542292089483661	5.772245657539592E-5
100-101	0.0	0.0012121715880833142	0.0	7.071650308122473	5.772245657539592E-5
102-103	0.0	0.0012121715880833142	0.0	7.616391561438628	5.772245657539592E-5
104-105	0.0	0.001342047115377955	0.0	8.15396079952529	5.772245657539592E-5
106-107	0.0	0.0014142001860972	0.0	8.7165238613091	5.772245657539592E-5
108-109	0.0	0.0014142001860972	0.0	9.307572955412866	5.772245657539592E-5
110-111	0.0	0.001443061414384898	0.0	9.938508267010231	5.772245657539592E-5
112-113	0.0	0.0015007838709602938	0.0	10.599617992782385	5.772245657539592E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	10415	0.0	27.82731	1
TCAACGC	10985	0.0	25.403824	4
CAACGCA	11185	0.0	25.001688	5
ATCAACG	11250	0.0	24.754316	3
AACGCAG	11365	0.0	24.553713	6
TATCAAC	11575	0.0	24.419485	2
GTACATG	14250	0.0	24.305784	1
TACATGG	13965	0.0	23.94745	2
CATGGGG	6980	0.0	23.44248	4
ACATGGG	14340	0.0	22.69843	3
ACGCAGA	12515	0.0	21.917137	7
CGCAGAG	12520	0.0	21.71829	8
GTATAGC	1995	0.0	20.582954	1
GTGTAGC	2790	0.0	19.41059	1
TACACCG	350	3.4118784E-8	18.69959	5
GCAGAGT	14270	0.0	18.34578	9
GACCGCG	390	5.9008016E-9	18.307556	7
TAGCCCT	3585	0.0	17.261179	4
GGTATCA	8065	0.0	17.193056	1
GAGTACT	4105	0.0	17.028967	12-13
>>END_MODULE