FastQCFastQC Report
Mon 15 Aug 2016
SRR2920953_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920953_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3464856
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40490.11685911333688905No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA126850.032.2701261
TCAACGC146450.027.0890454
ATCAACG148150.026.9792393
CAACGCA147850.026.801165
AACGCAG151050.026.348886
TATCAAC152900.026.2912352
GGTATCA92050.024.338761
ACGCAGA170350.023.330087
CGCAGAG172200.022.9418968
AGTACTT90100.021.66206712-13
CATGGGG68850.021.3698674
GTACATG148800.021.3431571
TACATGG147800.021.1543452
GAGTACT81000.020.75316212-13
ACATGGG151700.020.1044963
GCAGAGT189800.019.9373689
AGAGTAC169750.018.98079710-11
GTACTTT92450.018.0541414-15
GTATACG3354.350677E-717.7864631
GACCGTG10800.017.0773227
Produced by FastQC (version 0.11.5)