##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920953_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3464856
Sequences flagged as poor quality	0
Sequence length	125
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.043135125961946	31.0	30.0	34.0	26.0	34.0
2	30.150386047789578	31.0	30.0	34.0	26.0	34.0
3	30.259654658086802	31.0	30.0	34.0	26.0	34.0
4	33.80595643801647	35.0	35.0	37.0	30.0	37.0
5	33.32628484416091	35.0	35.0	37.0	27.0	37.0
6	33.09889011260497	35.0	33.0	37.0	26.0	37.0
7	32.95097920375334	35.0	33.0	37.0	26.0	37.0
8	32.89045836248317	35.0	33.0	37.0	26.0	37.0
9	34.2272740916217	38.0	34.0	39.0	26.0	39.0
10-11	34.12379057022861	38.0	34.0	39.0	25.5	39.0
12-13	34.02699982336928	38.0	33.5	39.0	25.0	39.0
14-15	34.93928694294944	39.0	34.0	41.0	25.0	41.0
16-17	34.93914725460452	39.0	34.0	41.0	25.0	41.0
18-19	34.996224085503115	39.0	34.0	41.0	25.0	41.0
20-21	34.971971706760684	39.0	34.0	41.0	25.0	41.0
22-23	34.937713573089326	39.0	34.0	41.0	25.0	41.0
24-25	34.916694517751964	39.0	34.0	41.0	24.5	41.0
26-27	34.771545484141335	39.0	34.0	41.0	24.0	41.0
28-29	34.65802821242788	39.0	34.0	41.0	23.5	41.0
30-31	34.498712789218374	38.5	33.5	41.0	23.0	41.0
32-33	34.364000697287274	38.0	33.0	41.0	22.0	41.0
34-35	34.24187354395103	38.0	33.0	41.0	21.5	41.0
36-37	34.106975874322046	38.0	33.0	40.5	20.0	41.0
38-39	33.97399271427153	38.0	33.0	40.0	18.5	41.0
40-41	33.79909453668493	38.0	33.0	40.0	17.0	41.0
42-43	33.66509632723553	38.0	33.0	40.0	16.0	41.0
44-45	33.44928634840813	38.0	32.5	40.0	15.0	41.0
46-47	33.30683858145908	38.0	32.5	40.0	12.0	41.0
48-49	33.107500138533894	38.0	32.0	40.0	9.0	41.0
50-51	32.93118992535332	38.0	32.0	40.0	8.5	41.0
52-53	32.71429101238262	38.0	31.5	40.0	8.0	41.0
54-55	32.558105012156346	38.0	31.0	40.0	7.0	41.0
56-57	32.45192541912276	37.0	31.0	40.0	4.0	41.0
58-59	32.41845101210555	37.0	31.0	40.0	2.0	41.0
60-61	32.18196412780213	37.0	31.0	40.0	2.0	41.0
62-63	31.94937870433865	36.5	31.0	40.0	2.0	41.0
64-65	31.70526726074619	36.0	31.0	39.5	2.0	41.0
66-67	31.41034778934536	36.0	30.0	39.0	2.0	41.0
68-69	31.10569097243868	35.0	30.0	39.0	2.0	41.0
70-71	30.766211063316916	35.0	30.0	39.0	2.0	40.0
72-73	30.477329216567732	35.0	29.5	38.0	2.0	40.0
74-75	30.198361923266077	35.0	29.0	37.0	2.0	39.5
76-77	29.84430983567571	35.0	29.0	37.0	2.0	39.0
78-79	29.492825675872243	34.5	29.0	36.0	2.0	39.0
80-81	29.20456218093912	34.0	29.0	36.0	2.0	37.5
82-83	28.918786956802823	34.0	29.0	35.0	2.0	37.0
84-85	28.685370041352368	34.0	29.0	35.0	2.0	37.0
86-87	28.42724762587536	34.0	29.0	35.0	2.0	36.0
88-89	28.273829994666446	34.0	28.5	35.0	2.0	36.0
90-91	28.067918695611013	34.0	27.5	35.0	2.0	36.0
92-93	28.178183595508735	34.0	29.0	35.0	2.0	35.0
94-95	28.106103399390914	34.0	29.0	35.0	2.0	35.0
96-97	28.031517471433155	34.0	29.0	35.0	2.0	35.0
98-99	27.974714677897147	34.0	28.5	35.0	2.0	35.0
100-101	27.843596097500154	34.0	28.0	35.0	2.0	35.0
102-103	26.568146266396063	32.0	25.0	34.0	2.0	35.0
104-105	27.607004446938056	34.0	27.0	35.0	2.0	35.0
106-107	27.79706689109158	34.0	28.0	35.0	2.0	35.0
108-109	27.779483187757297	34.0	28.5	35.0	2.0	35.0
110-111	27.723375083986177	34.0	28.0	35.0	2.0	35.0
112-113	27.62458771735391	34.0	27.0	35.0	2.0	35.0
114-115	27.543729955876955	34.0	27.0	35.0	2.0	35.0
116-117	27.423314417684313	34.0	27.0	35.0	2.0	35.0
118-119	27.302106783081317	34.0	27.0	35.0	2.0	35.0
120-121	27.16926316706957	34.0	27.0	35.0	2.0	35.0
122-123	26.945194980686068	34.0	27.0	35.0	2.0	35.0
124-125	25.862926049451982	33.0	22.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	113156.0
3	36616.0
4	17622.0
5	13452.0
6	14845.0
7	17526.0
8	19756.0
9	21642.0
10	23001.0
11	24314.0
12	25453.0
13	27507.0
14	30268.0
15	26182.0
16	20076.0
17	17875.0
18	16162.0
19	15315.0
20	15167.0
21	15762.0
22	18141.0
23	22793.0
24	30961.0
25	41224.0
26	50368.0
27	59202.0
28	69462.0
29	82225.0
30	95969.0
31	114800.0
32	139945.0
33	173793.0
34	221836.0
35	301763.0
36	478470.0
37	749622.0
38	301938.0
39	647.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.6990977567365	20.342820676083264	14.349060867129557	24.609020700050678
2	15.248341269706126	21.0060236391254	39.94543485156207	23.8002002396064
3	18.477880959081038	25.12509470311925	30.112489266979818	26.284535070819892
4	12.610046263787389	17.417261218462748	37.73206302971459	32.240629488035275
5	13.040934305187154	38.08348971381578	35.38009189139415	13.495484089602913
6	31.57324031345145	37.842269304910936	17.409277763459084	13.175212618178534
7	27.282619299548994	32.65647467790695	21.820472990017553	18.240433032526507
8	25.03248685660581	36.8943892846195	19.86628288590662	18.20684097286806
9	25.59978475446102	16.427888269782105	19.873144856841222	38.09918211891565
10-11	24.341335342495807	26.78253991622716	28.561630192862275	20.314494548414753
12-13	25.275188732186198	23.984258269229436	28.5380356841816	22.202517314402765
14-15	22.338623802477603	25.63834300387385	26.131577378542296	25.89145581510625
16-17	22.127951980112975	28.73791887838109	26.861615127765752	22.272514013740178
18-19	21.999302145663883	27.784462621122984	28.73603623645453	21.480198996758602
20-21	22.93249605631961	26.91744273706791	28.57751964334609	21.57254156326639
22-23	22.333122499475323	26.812018857842457	28.254950963085406	22.599907679596814
24-25	22.170714252928843	26.97594253714044	28.462801533797855	22.390541676132862
26-27	22.23393916807407	27.22141289335223	28.351339597649446	22.19330834092425
28-29	22.176978952040386	27.28032071706678	28.449869916176223	22.092830414716612
30-31	22.58301889543129	27.020826155305787	28.347448336605336	22.048706612657586
32-33	22.1681468237283	27.096480201086003	28.302459259053975	22.43291371613173
34-35	22.391814054894866	27.16621252226034	28.29470014416084	22.147273278683958
36-37	22.482644991244367	27.024892967040675	28.27920564575674	22.21325639595822
38-39	22.40964398045879	27.143362993328196	28.226594450273858	22.220398575939157
40-41	22.598270043046824	27.11600700990568	28.115091795696472	22.17063115135102
42-43	22.565731675665127	26.993314453938677	28.092285191069426	22.348668679326774
44-45	22.643052054307294	27.05938835582665	27.921768416822466	22.37579117304359
46-47	22.546185438155884	27.03553230169217	27.814828336741005	22.603453923410942
48-49	22.58903615433506	27.1885548009679	27.75153874724271	22.470870297454322
50-51	22.634764679179487	27.34515043457112	27.517391144318893	22.5026937419305
52-53	22.631692603319337	27.32517587498672	27.405087556411644	22.6380439652823
54-55	22.592654289568888	27.29118591543359	27.387988150459485	22.728171644538037
56-57	22.61692508884315	27.264785780477315	27.363054529917985	22.755234600761554
58-59	22.507215900790964	27.267660697056883	27.52907290614739	22.696050496004766
60-61	22.718751899304692	27.282323621453074	27.440966542353788	22.557957936888442
62-63	22.398977918523972	27.31441710661081	27.538766242897676	22.747838731967533
64-65	22.359492896054952	27.234135316745068	27.512316319908876	22.894055467291103
66-67	22.52451106354254	27.352955272903024	27.294604534438484	22.82792912911595
68-69	22.614501337530594	27.119161764119998	27.39016110993387	22.876175788415537
70-71	22.429624706483903	26.992062325741735	27.510149095928554	23.06816387184581
72-73	22.598099961079924	27.050406697487055	27.429646474037305	22.921846867395715
74-75	22.555182417145904	27.104720510414083	27.325764843125498	23.014332229314512
76-77	22.546445591894244	26.989971730088016	27.422536313110417	23.04104636490732
78-79	22.54351672723147	27.072495592246977	27.414361696445766	22.96962598407579
80-81	22.478689783437247	27.073310320488623	27.380988638159703	23.067011257914423
82-83	22.441505272032245	27.11383663051889	27.337796948804122	23.106861148644747
84-85	22.495646431806126	27.06453673199124	27.210591457407922	23.229225378794712
86-87	22.376820757556402	26.96184213280442	27.28170216697306	23.379634942666122
88-89	22.429159008125787	27.00394416358918	27.295523274000782	23.271373554284253
90-91	22.373552932562625	27.054202757039334	27.28253171588411	23.289712594513926
92-93	22.24676877970426	27.068577131410226	27.29869088341336	23.38596320547215
94-95	22.179682333117498	27.06224962609001	27.32449902123774	23.43356901955475
96-97	22.241973389904167	26.95770740325757	27.337765029646715	23.46255417719155
98-99	22.2217154213149	26.97235806812049	27.161850285847738	23.64407622471687
100-101	22.127185948956683	27.05667640204284	27.189254384888677	23.626883264111797
102-103	22.14519111642048	27.002336819243876	27.18302928508401	23.669442779251632
104-105	22.10379238347769	26.992832671643114	27.081675499226687	23.821699445652513
106-107	21.882398175344665	27.10149493747051	27.19027123750352	23.825835649681302
108-109	21.952362903996352	27.132412860220427	27.044630081175256	23.87059415460796
110-111	21.864659470602422	27.17099065131343	26.975887752829443	23.988462125254703
112-113	21.75835407904553	27.282517848480698	26.953294978120844	24.005833094352926
114-115	21.700437171562054	27.330034941972652	26.860591487783296	24.10893639868199
116-117	21.702253745707008	27.15704691709825	26.978904653838537	24.161794683356206
118-119	21.672338842333527	27.134065294626495	26.904296719970976	24.289299143069
120-121	21.66738751547588	27.230392082583442	26.86605676651902	24.236163635421658
122-123	21.535110834545073	27.236981176512693	26.86015353648238	24.367754452459856
124-125	21.403521387796395	27.34559074209408	26.721255065317926	24.529632804791603
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	83.0
1	87.5
2	161.0
3	279.0
4	273.5
5	201.0
6	222.0
7	439.0
8	696.0
9	760.0
10	629.5
11	525.0
12	591.0
13	701.5
14	829.0
15	973.5
16	1165.5
17	1345.5
18	1596.5
19	1920.5
20	2331.0
21	2885.0
22	3699.0
23	4913.5
24	6369.0
25	8484.0
26	11617.5
27	15380.5
28	19613.0
29	25621.0
30	32869.5
31	40625.5
32	50587.5
33	61348.5
34	71344.0
35	80104.0
36	89153.5
37	98195.5
38	105076.0
39	111351.0
40	118006.5
41	123961.5
42	128679.5
43	134021.0
44	139321.0
45	142885.5
46	144883.0
47	145772.0
48	146841.5
49	147691.0
50	149227.0
51	142670.0
52	128172.0
53	117889.5
54	108878.0
55	98525.0
56	87194.5
57	76141.0
58	67084.5
59	57918.5
60	47395.5
61	37508.5
62	29445.5
63	22424.0
64	16775.5
65	12687.0
66	9361.5
67	6744.5
68	5059.5
69	3835.0
70	2951.0
71	2301.0
72	1842.0
73	1651.5
74	1314.5
75	953.5
76	677.5
77	424.0
78	271.0
79	157.5
80	88.5
81	53.0
82	38.0
83	28.0
84	15.5
85	10.0
86	10.0
87	8.5
88	8.0
89	7.5
90	4.0
91	4.0
92	3.0
93	1.0
94	3.0
95	6.0
96	9.5
97	7.5
98	2.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05255629671189799
2	2.020285980138857E-4
3	0.0023377594913035348
4	0.1231219998753195
5	0.01584481432994618
6	0.0022223145781527427
7	0.005021853722059445
8	0.010909544292749828
9	0.026119411600366654
10-11	0.020953251736868718
12-13	0.019856525061936196
14-15	0.02844274047752634
16-17	0.03725984571941807
18-19	0.03990064810774243
20-21	0.02220871516738358
22-23	0.0240414031636524
24-25	0.02236745192296592
26-27	0.021285155862177243
28-29	0.021458323231903432
30-31	0.025123699224441073
32-33	0.024979393083002586
34-35	0.029337438554444976
36-37	0.03135772453458383
38-39	0.0317329205023239
40-41	0.03422941674920978
42-43	0.03218026954078322
44-45	0.029207563027150333
46-47	0.0388327826610976
48-49	0.031184557164857647
50-51	0.0233343030706038
52-53	0.029784787592904295
54-55	0.03154532251845387
56-57	0.02557104826290039
58-59	0.0282262812653686
60-61	0.04091079109781186
62-63	0.04984334125285437
64-65	0.042238407599045964
66-67	0.03803909888318591
68-69	0.029149840570574938
70-71	0.03598995167475936
72-73	0.038904935731816845
74-75	0.024387737903104775
76-77	0.02161705998748577
78-79	0.030968097952699912
80-81	0.02448875220211172
82-83	0.037404151860856556
84-85	0.021458323231903432
86-87	0.0332914268298596
88-89	0.03262761857924254
90-91	0.03500866991297762
92-93	0.02646574633981903
94-95	0.022713786662418294
96-97	0.018601061631421337
98-99	0.024863948169851793
100-101	0.024734072642557153
102-103	0.02769234854204619
104-105	0.025210282909304164
106-107	0.015310881606623767
108-109	0.01798054522323583
110-111	0.022699356048274447
112-113	0.014012126333677358
114-115	0.015613924503644596
116-117	0.014142001860972
118-119	0.008167727605418522
120-121	0.005498063988806461
122-123	0.00409829441685311
124-125	0.06394205127139482
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	3464856.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.126152522777645
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.03256333424414	34.10210646207852
2	14.94573175911708	14.983440594440491
3	6.491465775903409	9.761766107379763
4	3.474053168886018	6.965636760632782
5	2.0172913941548716	5.055952805314692
6	1.309567250249233	3.938614063489654
7	0.8831592180029673	3.098856156445964
8	0.5832948381250321	2.3390660817283404
9	0.43073319220620304	1.9431897920235925
>10	1.7701542027363726	14.038612943874002
>50	0.04359538734411659	1.4759761369737592
>100	0.017349124738003208	1.5028050276892235
>500	4.6282413093048284E-4	0.17495393038500284
>1k	5.785301617025172E-4	0.6190231375441654
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4049	0.11685911333688905	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	1.443061414384898E-5	0.0
12-13	0.0	1.443061414384898E-5	0.0	8.658368486309388E-5	0.0
14-15	0.0	1.1544491315079184E-4	0.0	1.298755272946408E-4	0.0
16-17	0.0	1.443061414384898E-4	0.0	2.0202859801388574E-4	0.0
18-19	0.0	1.443061414384898E-4	0.0	2.741816687331306E-4	2.886122828769796E-5
20-21	0.0	1.443061414384898E-4	0.0	3.319041253085265E-4	2.886122828769796E-5
22-23	0.0	1.443061414384898E-4	0.0	5.050714950347143E-4	2.886122828769796E-5
24-25	0.0	1.443061414384898E-4	0.0	7.648225496239959E-4	2.886122828769796E-5
26-27	0.0	1.443061414384898E-4	0.0	0.001356477729521804	2.886122828769796E-5
28-29	0.0	1.5873675558233878E-4	0.0	0.0031603044975029265	2.886122828769796E-5
30-31	0.0	1.7316736972618777E-4	0.0	0.008889258312610972	2.886122828769796E-5
32-33	0.0	1.7316736972618777E-4	0.0	0.02307455201601452	2.886122828769796E-5
34-35	0.0	1.8759798387003672E-4	0.0	0.04059331758664718	2.886122828769796E-5
36-37	0.0	2.7418166873313063E-4	0.0	0.06905048867831737	2.886122828769796E-5
38-39	0.0	3.4633473945237553E-4	0.0	0.11719101746219757	2.886122828769796E-5
40-41	0.0	3.7519596774007345E-4	0.0	0.1710027776046104	2.886122828769796E-5
42-43	0.0	4.329184243154694E-4	0.0	0.22254893132643896	2.886122828769796E-5
44-45	0.0	4.329184243154694E-4	0.0	0.28294105151844695	2.886122828769796E-5
46-47	0.0	4.762102667470163E-4	0.0	0.35554147127615116	2.886122828769796E-5
48-49	0.0	5.483633374662612E-4	0.0	0.43219689360827696	2.886122828769796E-5
50-51	0.0	6.060857940416572E-4	0.0	0.5109303243771169	2.886122828769796E-5
52-53	0.0	6.349470223293551E-4	0.0	0.595306125276202	2.886122828769796E-5
54-55	0.0	6.349470223293551E-4	0.0	0.6868683720189237	2.886122828769796E-5
56-57	0.0	6.349470223293551E-4	0.0	0.7959638149464221	2.886122828769796E-5
58-59	0.0	6.349470223293551E-4	0.0	0.9136166120612228	2.886122828769796E-5
60-61	0.0	6.63808250617053E-4	0.0	1.0343286993745195	2.886122828769796E-5
62-63	0.0	6.63808250617053E-4	0.0	1.160105932252307	2.886122828769796E-5
64-65	0.0	6.63808250617053E-4	0.0	1.2980337422392156	2.886122828769796E-5
66-67	0.0	6.63808250617053E-4	0.0	1.4557892160597727	2.886122828769796E-5
68-69	0.0	6.63808250617053E-4	0.0	1.639245613670525	2.886122828769796E-5
70-71	0.0	6.63808250617053E-4	0.0	1.8385323949970793	2.886122828769796E-5
72-73	0.0	6.63808250617053E-4	0.0	2.0382232335196613	2.886122828769796E-5
74-75	0.0	6.63808250617053E-4	0.0	2.250858332929276	2.886122828769796E-5
76-77	0.0	7.21530707192449E-4	0.0	2.485658855663843	2.886122828769796E-5
78-79	0.0	7.21530707192449E-4	0.0	2.751283170209671	2.886122828769796E-5
80-81	0.0	7.21530707192449E-4	0.0	3.0341664992715427	2.886122828769796E-5
82-83	0.0	7.21530707192449E-4	0.0	3.330845495454934	2.886122828769796E-5
84-85	0.0	7.21530707192449E-4	0.0	3.6348985354658314	2.886122828769796E-5
86-87	0.0	7.21530707192449E-4	0.0	3.962444615302916	2.886122828769796E-5
88-89	0.0	7.21530707192449E-4	0.0	4.334234958105041	2.886122828769796E-5
90-91	0.0	7.21530707192449E-4	0.0	4.729705938717222	2.886122828769796E-5
92-93	0.0	7.359613213362979E-4	0.0	5.138914863994348	2.886122828769796E-5
94-95	0.0	7.503919354801469E-4	0.0	5.563939742373131	2.886122828769796E-5
96-97	0.0	7.503919354801469E-4	0.0	5.994145211229558	2.886122828769796E-5
98-99	0.0	7.503919354801469E-4	0.0	6.47416516011055	2.886122828769796E-5
100-101	0.0	7.503919354801469E-4	0.0	7.002643111286588	2.886122828769796E-5
102-103	0.0	7.503919354801469E-4	0.0	7.546489666525824	2.886122828769796E-5
104-105	0.0	8.514062344870898E-4	0.0	8.084953602689405	2.886122828769796E-5
106-107	0.0	9.235593052063346E-4	0.0	8.647199190962048	2.886122828769796E-5
108-109	0.0	9.524205334940326E-4	0.0	9.238868801474	2.886122828769796E-5
110-111	0.0	9.668511476378816E-4	0.0	9.86989069675623	2.886122828769796E-5
112-113	0.0	9.812817617817307E-4	0.0	10.531664230779	2.886122828769796E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	12685	0.0	32.270126	1
TCAACGC	14645	0.0	27.089045	4
ATCAACG	14815	0.0	26.979239	3
CAACGCA	14785	0.0	26.80116	5
AACGCAG	15105	0.0	26.34888	6
TATCAAC	15290	0.0	26.291235	2
GGTATCA	9205	0.0	24.33876	1
ACGCAGA	17035	0.0	23.33008	7
CGCAGAG	17220	0.0	22.941896	8
AGTACTT	9010	0.0	21.662067	12-13
CATGGGG	6885	0.0	21.369867	4
GTACATG	14880	0.0	21.343157	1
TACATGG	14780	0.0	21.154345	2
GAGTACT	8100	0.0	20.753162	12-13
ACATGGG	15170	0.0	20.104496	3
GCAGAGT	18980	0.0	19.937368	9
AGAGTAC	16975	0.0	18.980797	10-11
GTACTTT	9245	0.0	18.05414	14-15
GTATACG	335	4.350677E-7	17.786463	1
GACCGTG	1080	0.0	17.077322	7
>>END_MODULE