FastQCFastQC Report
Mon 15 Aug 2016
SRR2920952_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920952_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3488048
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA77250.038.830881
TCAACGC87700.032.9757464
TATCAAC91350.032.0499232
ATCAACG90450.032.0394173
CAACGCA90500.031.888385
AACGCAG92650.030.9543916
GGTATCA55150.028.3828121
GTACATG124000.028.0308551
ACGCAGA105150.027.1044547
CGCAGAG105950.026.8436368
TACATGG126400.026.5052412
ACATGGG128600.025.26483
CATGGGG76300.025.1124634
GCAGAGT118800.022.5875059
GTGTAGC29350.019.264631
GTATATA21800.019.1111451
AGAGTAC104450.019.05415210-11
TACCGTA4851.4551915E-1118.4019557
GTACCGT6250.018.0881526
TAGCCCT32300.018.0543374
Produced by FastQC (version 0.11.5)