##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920952_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3488048
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.440040102659136	31.0	28.0	33.0	16.0	34.0
2	28.524314458975336	31.0	28.0	34.0	16.0	34.0
3	28.600309399411934	31.0	28.0	34.0	16.0	34.0
4	32.053865944505354	35.0	32.0	37.0	19.0	37.0
5	32.13062148227318	35.0	33.0	37.0	20.0	37.0
6	32.06528923913891	35.0	32.0	37.0	19.0	37.0
7	31.9473421810709	35.0	32.0	37.0	18.0	37.0
8	31.90509390925813	35.0	32.0	37.0	18.0	37.0
9	33.11048729833993	37.0	33.0	39.0	17.0	39.0
10-11	33.043806449911244	37.0	33.0	39.0	17.0	39.0
12-13	32.97917001142186	37.0	33.0	39.0	17.0	39.0
14-15	33.86962894432645	38.0	33.0	41.0	17.0	41.0
16-17	33.742583961000534	38.0	32.5	41.0	16.5	41.0
18-19	33.85272880419077	38.5	33.0	41.0	17.5	41.0
20-21	33.87819333334862	39.0	33.0	41.0	17.5	41.0
22-23	33.83274570762788	38.5	33.0	41.0	17.5	41.0
24-25	33.76251817635537	38.0	32.5	41.0	16.5	41.0
26-27	33.61740090732697	38.0	32.0	41.0	16.0	41.0
28-29	33.541794436315094	38.0	32.0	40.0	15.5	41.0
30-31	33.416773937743976	38.0	32.0	40.0	12.5	41.0
32-33	33.31113003605455	38.0	32.0	40.0	10.0	41.0
34-35	33.13608456649679	38.0	32.0	40.0	9.0	41.0
36-37	32.9847871932955	38.0	31.0	40.0	9.0	41.0
38-39	32.86121793622106	38.0	31.0	40.0	8.5	41.0
40-41	32.78483380962648	38.0	31.0	40.0	8.0	41.0
42-43	32.58418949509869	38.0	31.0	40.0	6.5	41.0
44-45	32.43017684963051	38.0	31.0	40.0	2.0	41.0
46-47	32.24589412186988	38.0	30.5	40.0	2.0	41.0
48-49	32.08411581491998	37.5	30.0	40.0	2.0	41.0
50-51	31.872390804254987	37.0	30.0	40.0	2.0	41.0
52-53	31.705012803722884	37.0	30.0	40.0	2.0	41.0
54-55	31.504448333279818	37.0	30.0	40.0	2.0	41.0
56-57	31.317524013431008	36.5	30.0	40.0	2.0	41.0
58-59	31.07487999018362	36.0	29.0	40.0	2.0	41.0
60-61	30.900802253868065	36.0	29.0	39.0	2.0	41.0
62-63	30.800370293069363	36.0	29.0	39.0	2.0	41.0
64-65	30.58894559363862	35.0	29.0	39.0	2.0	41.0
66-67	30.338614749567668	35.0	29.0	39.0	2.0	41.0
68-69	30.040616700228895	35.0	29.0	38.5	2.0	40.0
70-71	29.718613247294762	35.0	28.0	38.0	2.0	40.0
72-73	29.434852100659164	34.5	28.0	37.0	2.0	40.0
74-75	29.127794542965006	34.0	28.0	37.0	2.0	39.0
76-77	28.834936044458104	34.0	28.0	36.0	2.0	39.0
78-79	28.50234572460012	34.0	27.5	36.0	2.0	38.5
80-81	28.173340647835122	34.0	27.0	35.5	2.0	37.0
82-83	27.88512801429338	34.0	26.0	35.0	2.0	37.0
84-85	27.628910496644544	34.0	26.0	35.0	2.0	36.5
86-87	27.535678121402	34.0	26.0	35.0	2.0	36.0
88-89	27.602408424425352	34.0	27.0	35.0	2.0	36.0
90-91	27.500555898313326	34.0	27.0	35.0	2.0	36.0
92-93	27.34182843814076	34.0	26.0	35.0	2.0	35.0
94-95	27.252986770824254	34.0	26.0	35.0	2.0	35.0
96-97	27.157549437393065	34.0	26.0	35.0	2.0	35.0
98-99	27.095781365394053	34.0	26.0	35.0	2.0	35.0
100-101	26.928126419131846	34.0	25.0	35.0	2.0	35.0
102-103	25.637476892519828	31.5	23.5	34.0	2.0	35.0
104-105	26.628795819323585	33.0	25.0	35.0	2.0	35.0
106-107	26.81105434902272	34.0	25.0	35.0	2.0	35.0
108-109	26.809291901946303	34.0	25.0	35.0	2.0	35.0
110-111	26.713105295569328	34.0	25.0	35.0	2.0	35.0
112-113	26.623970771044434	34.0	25.0	35.0	2.0	35.0
114-115	26.504414360123484	34.0	25.0	35.0	2.0	35.0
116-117	26.378681142002634	34.0	25.0	35.0	2.0	35.0
118-119	26.20186734815576	34.0	24.0	35.0	2.0	35.0
120-121	25.99919854887318	34.0	23.5	35.0	2.0	35.0
122-123	25.786418936895366	34.0	23.0	35.0	2.0	35.0
124-125	24.67706078012688	32.5	10.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	203760.0
3	28838.0
4	17716.0
5	15512.0
6	17816.0
7	19493.0
8	21018.0
9	22643.0
10	23866.0
11	24657.0
12	25496.0
13	25891.0
14	26039.0
15	23711.0
16	20239.0
17	18126.0
18	16382.0
19	15642.0
20	15936.0
21	17051.0
22	20479.0
23	27132.0
24	37415.0
25	47625.0
26	56163.0
27	63212.0
28	74409.0
29	86388.0
30	100723.0
31	119559.0
32	146202.0
33	181863.0
34	233303.0
35	319266.0
36	488246.0
37	659169.0
38	226713.0
39	349.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.992693654297085	19.184749939199826	13.193265405388043	24.629291001115053
2	15.433851624049263	20.022624142454674	40.46332179186649	24.08020244162957
3	19.177122138396197	24.4573587948552	29.227508560472735	27.13801050627586
4	12.394016764620869	16.298707555090637	37.631102638569644	33.676173041718855
5	13.62899593098823	37.77379666525117	34.852515168832014	13.744692234928586
6	32.37957208902269	36.696899974913094	17.468171881159734	13.455356054904492
7	27.959542174484834	32.420620096052644	21.340705913249856	18.27913181621266
8	25.354855412923055	36.32044460895302	20.01482870863222	18.30987126949171
9	25.849970154354452	16.516884149009186	19.716560978868365	37.91658471776799
10-11	24.651235534209277	26.544546779652073	28.273861745402083	20.530355940736563
12-13	25.298716453578514	23.939262528859544	27.988522842854263	22.773498174707676
14-15	22.498730633014898	25.54952624156419	25.443776718705696	26.507966406715216
16-17	22.558014387160902	28.518757753433157	26.119784252793387	22.803443606612557
18-19	22.51724245688203	27.727278330825243	27.673566325224403	22.081912887068324
20-21	23.615122777416612	26.8807103379022	27.36287483863939	22.141292046041798
22-23	23.042074138591175	26.722541704516445	27.055638848556406	23.17974530833597
24-25	22.724955042320065	26.82832079392145	27.38793908547944	23.058785078279044
26-27	22.752781647797423	27.017982104621407	27.301354029843218	22.927882217737952
28-29	22.682523768306993	27.07367924303809	27.359420724478582	22.884376264176336
30-31	23.154550645060624	26.98317132814154	27.122118930145035	22.7401590966528
32-33	22.690789044587266	27.063020214030914	27.05916418856108	23.18702655282074
34-35	22.954745728476663	27.059080894416926	27.187828328535467	22.79834504857094
36-37	23.034686800104307	26.920435294891774	27.07597762656164	22.968900278442277
38-39	22.940210215294144	27.098601474686536	27.072956372793485	22.888231937225836
40-41	23.10344140503098	27.046823423738793	27.046164021675978	22.80357114955425
42-43	22.99122281022884	26.94006226846254	27.073965696008973	22.99474922529965
44-45	23.143269126152468	26.967618026978457	26.978397826494255	22.910715020374823
46-47	23.00642906330608	27.035830618892508	26.855336254448737	23.102404063352676
48-49	22.922864190791618	27.042502305077594	26.968189355816826	23.066444148313966
50-51	22.97558679038519	27.176605111331188	26.797788343155464	23.05001975512816
52-53	23.072784703522156	27.130218886651846	26.740281121590716	23.05671528823528
54-55	23.018452921319533	27.032213090537848	26.818396643270997	23.130937344871626
56-57	23.017751547146617	27.19649521764983	26.689748437085186	23.096004798118365
58-59	22.951550552622972	27.0261289211803	26.876909680858045	23.14541084533868
60-61	23.093801717162087	27.056677530033134	26.866240111696527	22.98328064110825
62-63	22.923143861278902	27.027805434356424	26.900028457006623	23.14902224735805
64-65	22.89459653694263	26.956515880028046	26.932947329019406	23.215940254009915
66-67	23.003690465354136	27.06764610809645	26.751480235690934	23.177183190858482
68-69	23.071849524136365	26.93235785266383	26.74524068273899	23.250551940460813
70-71	22.96011225131082	26.70543033491901	26.962046946461584	23.372410467308583
72-73	23.08369423864236	26.758527216745186	26.858408468875027	23.29937007573743
74-75	22.9876372464612	26.851366852476378	26.841676421604845	23.319319479457576
76-77	22.992227208683026	26.686335050697384	26.937639487150307	23.383798253469287
78-79	23.07898397104039	26.812523571683215	26.835143786730075	23.273348670546316
80-81	23.097545965570497	26.811174429841138	26.725865450350707	23.36541415423766
82-83	23.05041896586375	26.865463892846115	26.85720699554156	23.226910145748572
84-85	23.223742879697728	26.748927716917553	26.74543001199971	23.281899391385007
86-87	22.950767966505207	26.672108824812113	26.93614524884971	23.440977959832974
88-89	23.08953317156186	26.652700880263115	26.869914060815674	23.38785188735935
90-91	23.001503571430106	26.699968191200274	26.90674689154633	23.391781345823293
92-93	23.022825105106843	26.66428787183065	26.94342114373966	23.36946587932285
94-95	22.986454775878844	26.709380849349362	26.95136480816392	23.352799566607878
96-97	22.972365659051313	26.568882577525056	27.04852807077979	23.410223692643843
98-99	22.960646342021075	26.623893330229357	26.83716750451584	23.578292823233728
100-101	22.90832437993602	26.70195552519955	26.91027075819003	23.479449336674403
102-103	22.810845697097538	26.651541475027308	26.975271802589585	23.562341025285573
104-105	22.79762188062497	26.70006978615161	26.876384469513432	23.625923863709996
106-107	22.64134902044446	26.719344139512863	26.936666313973333	23.70264052606934
108-109	22.705649836739422	26.701878270802677	26.89599033855209	23.69648155390581
110-111	22.64720434789528	26.905146931579317	26.83017679274907	23.61747192777633
112-113	22.672790818989498	26.880562831024683	26.807926802608474	23.63871954737734
114-115	22.626028547728026	26.925477761978776	26.811659029528638	23.636834660764553
116-117	22.69561010224817	26.75951789611758	26.872983618072805	23.67188838356145
118-119	22.68051597002991	26.62803189447334	26.807295670116808	23.884156465379945
120-121	22.660833950365525	26.7836220422774	26.832019347453517	23.723524659903564
122-123	22.715206505721937	26.810427493516254	26.71192589055355	23.762440110208257
124-125	22.651642410306543	26.814594319835138	26.677392131484616	23.8563711383737
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	779.0
1	568.5
2	315.5
3	232.0
4	191.0
5	193.0
6	193.5
7	230.0
8	304.0
9	378.0
10	419.5
11	464.0
12	548.5
13	622.5
14	695.0
15	810.0
16	936.5
17	1093.0
18	1345.0
19	1629.0
20	2027.5
21	2688.5
22	3497.5
23	4576.0
24	6095.0
25	8274.5
26	11761.5
27	16355.5
28	21115.5
29	26791.0
30	33541.0
31	41245.0
32	49339.0
33	57511.5
34	65862.5
35	73387.0
36	82248.5
37	89902.5
38	94374.0
39	98728.0
40	105125.5
41	112016.0
42	115932.5
43	121142.0
44	127963.0
45	132510.0
46	135882.5
47	138843.5
48	141490.0
49	142310.5
50	140863.0
51	137168.5
52	130983.0
53	125883.5
54	120389.0
55	112959.0
56	103527.0
57	92774.0
58	84151.5
59	75376.5
60	64240.5
61	52637.5
62	42054.5
63	32093.0
64	23992.5
65	18157.0
66	13385.0
67	9896.5
68	7565.0
69	5736.0
70	4546.5
71	3741.5
72	2900.5
73	2329.5
74	1847.0
75	1386.5
76	1007.5
77	675.0
78	467.5
79	335.0
80	219.0
81	152.5
82	126.5
83	82.0
84	47.0
85	38.0
86	32.0
87	27.0
88	19.5
89	13.0
90	14.0
91	16.5
92	12.0
93	9.0
94	10.0
95	10.0
96	9.5
97	10.0
98	8.5
99	7.5
100	12.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03486190557010683
2	0.017860992738632037
3	0.022906794860621183
4	0.020183208487956586
5	0.013445915881891535
6	0.004959794131273423
7	0.004845116810319124
8	0.006278583322247859
9	0.0029242716843346193
10-11	0.0047017701591262505
12-13	0.007181667224762963
14-15	0.0025515703912331483
16-17	0.002207538428370252
18-19	2.86693302385747E-5
20-21	0.0037413475961339984
22-23	7.597372513222295E-4
24-25	0.0013044545258551487
26-27	0.0015051398375251718
28-29	0.0030102796750503436
30-31	0.0012184465351394247
32-33	1.433466511928735E-5
34-35	0.006063563345458549
36-37	0.007210336555001537
38-39	0.0015051398375251718
40-41	0.0011324385444237005
42-43	0.0024798970656367113
44-45	0.0012184465351394247
46-47	0.019538148557588658
48-49	0.002752255702903171
50-51	0.009919588262546845
52-53	0.0015911478282408958
54-55	2.723586372664596E-4
56-57	4.013706233400458E-4
58-59	0.006507937964156457
60-61	7.310679210836548E-4
62-63	0.009475213643848937
64-65	0.010019930918381856
66-67	1.5768131631216083E-4
68-69	0.0036266702751796996
70-71	0.004587092838171951
72-73	0.011654082741980615
74-75	0.0020355224469388034
76-77	0.0013617931863322981
78-79	5.017132791750572E-4
80-81	0.0039707022380425965
82-83	0.0015768131631216085
84-85	0.0013331238560937235
86-87	0.013216561239982936
88-89	0.0
90-91	5.590519396522066E-4
92-93	0.008242432443590225
94-95	0.006120902005935698
96-97	0.004572758173052664
98-99	0.0014191318468094476
100-101	0.015624784980023211
102-103	0.007697715169057307
104-105	0.007167332559643675
106-107	0.017172928812906243
108-109	0.010664990848749788
110-111	0.018907423292340013
112-113	0.0031392916611239297
114-115	0.01390462516570873
116-117	0.0060348940152199744
118-119	0.0049024554707962735
120-121	0.007181667224762963
122-123	0.008242432443590225
124-125	0.055962532625697815
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	3488048.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.99131540439578
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.79279890184881	35.58826621759739
2	14.282340192349189	14.565506269219119
3	6.123039384741516	9.366654975026762
4	3.169045541982803	6.463752030485578
5	1.8928965817535743	4.826064331404957
6	1.1907377281906562	3.643036983725006
7	0.790376361326466	2.8211631230003555
8	0.5493075504415253	2.240793164686389
9	0.4107356653761887	1.8849556674928436
>10	1.7167444764705289	14.395478550252008
>50	0.060902222199327824	2.1098910249532206
>100	0.020338832434774294	1.708310390885585
>500	3.966097103953316E-4	0.13867646226417102
>1k	3.399511742567821E-4	0.24745080900658314
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.86693302385747E-5	0.0
5	0.0	0.0	0.0	2.86693302385747E-5	0.0
6	0.0	0.0	0.0	2.86693302385747E-5	0.0
7	0.0	0.0	0.0	2.86693302385747E-5	0.0
8	0.0	0.0	0.0	2.86693302385747E-5	0.0
9	0.0	0.0	0.0	2.86693302385747E-5	0.0
10-11	2.86693302385747E-5	0.0	0.0	7.167332559643674E-5	0.0
12-13	2.86693302385747E-5	0.0	0.0	1.7201598143144818E-4	2.86693302385747E-5
14-15	2.86693302385747E-5	0.0	0.0	1.7201598143144818E-4	4.3003995357862045E-5
16-17	2.86693302385747E-5	0.0	0.0	3.5836662798218377E-4	5.73386604771494E-5
18-19	2.86693302385747E-5	0.0	0.0	5.160479442943446E-4	5.73386604771494E-5
20-21	2.86693302385747E-5	0.0	0.0	5.877212698907814E-4	5.73386604771494E-5
22-23	2.86693302385747E-5	0.0	0.0	7.310679210836548E-4	8.600799071572409E-5
24-25	2.86693302385747E-5	0.0	0.0	0.0011611078746622753	1.146773209542988E-4
26-27	2.86693302385747E-5	0.0	0.0	0.00167715581895662	1.146773209542988E-4
28-29	2.86693302385747E-5	0.0	0.0	0.0033113076425553777	1.146773209542988E-4
30-31	2.86693302385747E-5	0.0	0.0	0.009231524336821053	1.146773209542988E-4
32-33	2.86693302385747E-5	0.0	0.0	0.02362352811658555	1.146773209542988E-4
34-35	2.86693302385747E-5	0.0	0.0	0.04281764471131132	1.146773209542988E-4
36-37	2.86693302385747E-5	0.0	0.0	0.07518531855066214	1.146773209542988E-4
38-39	2.86693302385747E-5	0.0	0.0	0.12723448759879452	1.146773209542988E-4
40-41	2.86693302385747E-5	0.0	0.0	0.184028430801411	1.2901198607358615E-4
42-43	2.86693302385747E-5	0.0	0.0	0.23746806236611423	1.433466511928735E-4
44-45	2.86693302385747E-5	0.0	0.0	0.30091329018408003	1.7201598143144818E-4
46-47	2.86693302385747E-5	0.0	0.0	0.37660032201391724	2.293546419085976E-4
48-49	2.86693302385747E-5	0.0	0.0	0.4588239611381495	2.4368930702788495E-4
50-51	2.86693302385747E-5	0.0	0.0	0.5465521116681881	2.580239721471723E-4
52-53	2.86693302385747E-5	0.0	0.0	0.634294596863346	2.580239721471723E-4
54-55	2.86693302385747E-5	0.0	0.0	0.7315839690279491	2.580239721471723E-4
56-57	2.86693302385747E-5	0.0	0.0	0.8439104049026849	2.7235863726645965E-4
58-59	2.86693302385747E-5	0.0	0.0	0.9653966917886452	3.153626326243217E-4
60-61	2.86693302385747E-5	0.0	0.0	1.0978489974908603	3.153626326243217E-4
62-63	2.86693302385747E-5	0.0	0.0	1.234372348086953	3.4403196286289636E-4
64-65	2.86693302385747E-5	0.0	0.0	1.3796685137360494	3.4403196286289636E-4
66-67	2.86693302385747E-5	0.0	0.0	1.5458216171337091	3.4403196286289636E-4
68-69	2.86693302385747E-5	0.0	0.0	1.7332473635683914	3.583666279821837E-4
70-71	2.86693302385747E-5	0.0	0.0	1.9384337600858703	3.7270129310147107E-4
72-73	4.3003995357862045E-5	0.0	0.0	2.146730778934235	4.4437461869790783E-4
74-75	8.600799071572409E-5	0.0	0.0	2.362137791681766	4.587092838171952E-4
76-77	8.600799071572409E-5	0.0	0.0	2.6026734723834073	4.587092838171952E-4
78-79	8.600799071572409E-5	0.0	0.0	2.8660586092851936	4.587092838171952E-4
80-81	1.146773209542988E-4	0.0	0.0	3.1501143332889914	4.7304394893648254E-4
82-83	1.146773209542988E-4	0.0	0.0	3.453650867189901	4.873786140557699E-4
84-85	1.146773209542988E-4	0.0	0.0	3.760842740696229	4.873786140557699E-4
86-87	1.146773209542988E-4	0.0	0.0	4.091471791672591	4.873786140557699E-4
88-89	1.146773209542988E-4	0.0	0.0	4.456317688288694	4.873786140557699E-4
90-91	1.146773209542988E-4	0.0	0.0	4.847955074012743	4.873786140557699E-4
92-93	1.146773209542988E-4	0.0	0.0	5.26034905482952	4.873786140557699E-4
94-95	1.146773209542988E-4	0.0	0.0	5.679709682894272	5.447172745329193E-4
96-97	1.146773209542988E-4	0.0	0.0	6.108445181947037	5.590519396522066E-4
98-99	1.146773209542988E-4	0.0	0.0	6.576629679408082	5.73386604771494E-4
100-101	1.2901198607358615E-4	0.0	0.0	7.093480364949107	5.73386604771494E-4
102-103	1.433466511928735E-4	0.0	0.0	7.634183933248625	5.73386604771494E-4
104-105	1.433466511928735E-4	0.0	0.0	8.172894983096562	5.73386604771494E-4
106-107	1.433466511928735E-4	0.0	0.0	8.713899579363588	5.877212698907814E-4
108-109	1.433466511928735E-4	0.0	0.0	9.281222620789622	6.020559350100687E-4
110-111	1.433466511928735E-4	0.0	0.0	9.894086893299633	6.593945954872181E-4
112-113	1.433466511928735E-4	0.0	0.0	10.54303151791489	6.880639257257927E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7725	0.0	38.83088	1
TCAACGC	8770	0.0	32.975746	4
TATCAAC	9135	0.0	32.049923	2
ATCAACG	9045	0.0	32.039417	3
CAACGCA	9050	0.0	31.88838	5
AACGCAG	9265	0.0	30.954391	6
GGTATCA	5515	0.0	28.382812	1
GTACATG	12400	0.0	28.030855	1
ACGCAGA	10515	0.0	27.104454	7
CGCAGAG	10595	0.0	26.843636	8
TACATGG	12640	0.0	26.505241	2
ACATGGG	12860	0.0	25.2648	3
CATGGGG	7630	0.0	25.112463	4
GCAGAGT	11880	0.0	22.587505	9
GTGTAGC	2935	0.0	19.26463	1
GTATATA	2180	0.0	19.111145	1
AGAGTAC	10445	0.0	19.054152	10-11
TACCGTA	485	1.4551915E-11	18.401955	7
GTACCGT	625	0.0	18.088152	6
TAGCCCT	3230	0.0	18.054337	4
>>END_MODULE