FastQCFastQC Report
Mon 15 Aug 2016
SRR2920952_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920952_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3488048
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42250.12112792025797811No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34940.10017063985358No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA99000.045.751591
GGTATCA62900.036.7628251
TCAACGC120650.036.6399354
CAACGCA123050.035.779655
ATCAACG124850.035.406843
TATCAAC125350.035.309592
AACGCAG128700.034.342676
ACGCAGA145600.029.9104737
CGCAGAG147950.029.5170758
GCAGAGT165950.025.0619
GTACATG129900.024.591411
TACATGG131400.023.8461672
CATGGGG80050.023.8158864
AGAGTAC146350.023.27554910-11
GAGTACT84900.022.63891412-13
ACATGGG135150.022.3052123
AGTACTT92800.021.73764412-13
GTACTTT93550.020.51382614-15
CAGAGTA155250.020.35073510-11
GTGTAGC26650.018.5613121
Produced by FastQC (version 0.11.5)