FastQCFastQC Report
Mon 15 Aug 2016
SRR2920951_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920951_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3740643
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA100100.030.9736121
TCAACGC105100.028.1924974
CAACGCA107150.027.76385
ATCAACG106650.027.7273433
GTACATG159500.027.7214741
AACGCAG111650.026.7517346
TACATGG162500.026.0705972
TATCAAC113400.026.0251332
ACATGGG163400.025.6714153
CATGGGG99750.024.0976874
ACGCAGA126600.023.2166827
CGCAGAG127600.022.8015888
GGTATCA73550.021.7649571
GTGTAGC30900.020.4143521
GTATAGC19800.019.8365881
GCAGAGT142500.019.164829
ATGGGGA62200.019.0354795
CGTATAC3554.0136E-818.4364383
TAGCCCT37200.018.0735054
GTATAGG12600.017.9473881
Produced by FastQC (version 0.11.5)