##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920951_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3740643
Sequences flagged as poor quality	0
Sequence length	125
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.2725207938849	31.0	28.0	33.0	16.0	34.0
2	28.353610863159087	31.0	28.0	34.0	16.0	34.0
3	28.438355919022477	31.0	28.0	34.0	16.0	34.0
4	31.887614241722613	35.0	32.0	37.0	19.0	37.0
5	31.95264129723152	35.0	32.0	37.0	19.0	37.0
6	31.88502431266496	35.0	32.0	37.0	18.0	37.0
7	31.766976693579153	35.0	32.0	37.0	17.0	37.0
8	31.72010721151417	35.0	32.0	37.0	17.0	37.0
9	32.92622631991345	37.0	32.0	39.0	17.0	39.0
10-11	32.849198921148044	37.0	32.0	39.0	17.0	39.0
12-13	32.77312069074755	37.0	32.0	39.0	16.5	39.0
14-15	33.64593921419392	38.0	32.0	41.0	16.0	41.0
16-17	33.50351089371533	38.0	32.0	40.0	14.0	41.0
18-19	33.60971308943409	38.0	32.0	41.0	16.0	41.0
20-21	33.63293449815981	38.0	32.0	41.0	15.5	41.0
22-23	33.574516065820774	38.0	32.0	40.5	15.0	41.0
24-25	33.4959123337886	38.0	32.0	40.5	13.5	41.0
26-27	33.34245235377982	38.0	32.0	40.0	10.5	41.0
28-29	33.26108599510833	38.0	32.0	40.0	10.0	41.0
30-31	33.13286124872114	38.0	32.0	40.0	9.5	41.0
32-33	33.03117953250283	38.0	32.0	40.0	9.0	41.0
34-35	32.860318667138245	38.0	31.0	40.0	8.5	41.0
36-37	32.71592317149752	38.0	31.0	40.0	8.0	41.0
38-39	32.5971520137046	38.0	31.0	40.0	7.5	41.0
40-41	32.52485013940117	38.0	31.0	40.0	2.0	41.0
42-43	32.317057254595	38.0	30.5	40.0	2.0	41.0
44-45	32.166725079083996	38.0	30.0	40.0	2.0	41.0
46-47	31.9848911002734	38.0	30.0	40.0	2.0	41.0
48-49	31.828788927465144	37.0	30.0	40.0	2.0	41.0
50-51	31.610716660210556	37.0	30.0	40.0	2.0	41.0
52-53	31.44018434798509	37.0	30.0	40.0	2.0	41.0
54-55	31.24185160144927	37.0	29.5	40.0	2.0	41.0
56-57	31.05054572168475	36.0	29.0	40.0	2.0	41.0
58-59	30.80628277010129	36.0	29.0	40.0	2.0	41.0
60-61	30.623749980952475	36.0	28.5	39.0	2.0	41.0
62-63	30.50623970263936	35.5	29.0	39.0	2.0	41.0
64-65	30.290869109936445	35.0	28.0	39.0	2.0	41.0
66-67	30.029918385689307	35.0	28.0	39.0	2.0	40.5
68-69	29.724339638933735	35.0	28.0	38.5	2.0	40.0
70-71	29.392829922556096	35.0	28.0	37.5	2.0	40.0
72-73	29.09867367722608	34.0	28.0	37.0	2.0	39.5
74-75	28.780763628071433	34.0	27.5	36.5	2.0	39.0
76-77	28.47774607199885	34.0	27.0	36.0	2.0	39.0
78-79	28.145686049163206	34.0	26.0	36.0	2.0	37.5
80-81	27.815029795679514	34.0	26.0	35.0	2.0	37.0
82-83	27.53360531865778	34.0	26.0	35.0	2.0	37.0
84-85	27.274249908371367	34.0	26.0	35.0	2.0	36.0
86-87	27.1842930480134	33.5	26.0	35.0	2.0	36.0
88-89	27.258620376229437	34.0	26.0	35.0	2.0	36.0
90-91	27.15933183145251	34.0	26.0	35.0	2.0	35.5
92-93	26.99775600077313	34.0	26.0	35.0	2.0	35.0
94-95	26.912067123219188	34.0	25.5	35.0	2.0	35.0
96-97	26.808875506162977	34.0	25.0	35.0	2.0	35.0
98-99	26.745865750888285	34.0	25.0	35.0	2.0	35.0
100-101	26.56843008006912	33.5	25.0	35.0	2.0	35.0
102-103	25.27614423509541	31.5	21.5	34.0	2.0	35.0
104-105	26.258280194073585	33.0	24.5	35.0	2.0	35.0
106-107	26.434337358577125	33.5	25.0	35.0	2.0	35.0
108-109	26.423852262832888	34.0	25.0	35.0	2.0	35.0
110-111	26.318349545786646	34.0	24.5	35.0	2.0	35.0
112-113	26.221131500653765	34.0	24.0	35.0	2.0	35.0
114-115	26.091914678839977	34.0	24.0	35.0	2.0	35.0
116-117	25.95655078552003	33.5	23.0	35.0	2.0	35.0
118-119	25.761154699873792	33.0	21.5	35.0	2.0	35.0
120-121	25.54973930952513	33.0	19.5	35.0	2.0	35.0
122-123	25.320899107452917	33.0	17.5	35.0	2.0	35.0
124-125	24.190902473184423	32.0	2.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	232396.0
3	32492.0
4	20402.0
5	17979.0
6	20148.0
7	21600.0
8	23608.0
9	25268.0
10	26156.0
11	27048.0
12	27416.0
13	28261.0
14	28065.0
15	25720.0
16	22758.0
17	19993.0
18	18628.0
19	18083.0
20	18232.0
21	19607.0
22	23614.0
23	30936.0
24	41222.0
25	52751.0
26	61801.0
27	70789.0
28	82231.0
29	95482.0
30	110233.0
31	131330.0
32	158619.0
33	195857.0
34	250846.0
35	337906.0
36	515220.0
37	691161.0
38	216487.0
39	298.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.25164055032849	18.73634434735541	13.132398789718058	24.879616312598046
2	15.532726208534239	19.81888397167535	40.17908924862891	24.469300571161494
3	19.273882243716383	24.265854913357245	28.77019801461284	27.69006482831353
4	12.589590064955136	16.14629291525884	36.8308086074915	34.43330841229452
5	13.854675291826402	37.74532321087382	34.46626342957585	13.933738067723938
6	33.26108698889409	35.89457184616902	17.32217280145694	13.522168363479942
7	28.673937848823716	32.011309935654424	20.87277177905844	18.44198043646342
8	25.906017229666595	35.9889787453725	19.710411348063808	18.394592676897098
9	26.47551361732889	16.112056907474027	19.10652720685422	38.30590226834287
10-11	24.91612286945271	26.34753143445767	27.885331183497993	20.85101451259163
12-13	25.61149994325369	23.68624278356076	27.718826526472316	22.983430746713225
14-15	22.89656088751083	25.187252925989878	25.15201726814576	26.764168918353526
16-17	23.123002378131616	28.02849533951167	25.874729634733367	22.973772647623345
18-19	22.87700269712988	27.53481152839231	27.121874501255533	22.466311273222278
20-21	23.954225838321644	26.703193922621725	26.855152440405938	22.487427798650685
22-23	23.443546401475913	26.647040597465164	26.511394018557972	23.39801898250095
24-25	23.054583854086605	26.672570393589222	26.885184764374458	23.38766098794971
26-27	23.224935534893632	26.81777832077003	26.69100685867829	23.266279285658047
28-29	23.114912289964902	26.982045752539978	26.7236870228875	23.179354934607623
30-31	23.582819543721406	26.767225736598867	26.63780756365143	23.0121471560283
32-33	23.238304216681463	26.863496463041248	26.638815839950514	23.25938348032678
34-35	23.301947097987455	26.933563291712865	26.67939173139036	23.085097878909323
36-37	23.424729610286974	26.66976709616393	26.697344788257332	23.208158505291763
38-39	23.298331237181987	26.853937705741522	26.654998261635775	23.192732795440712
40-41	23.545040732737476	26.761407184884362	26.58340112543352	23.110150956944643
42-43	23.40338902214328	26.672455479785317	26.639572597384127	23.28458290068728
44-45	23.426686016506178	26.77638972041781	26.532832504522336	23.264091758553676
46-47	23.252064563836868	26.73038786352387	26.580020206781935	23.43752736585733
48-49	23.33929149555664	26.79320320371969	26.54138039907211	23.32612490165156
50-51	23.28468007087776	26.860958881676787	26.5005544426598	23.35380660478565
52-53	23.358785686940863	26.884585401637818	26.285026616164746	23.47160229525657
54-55	23.30807067840247	26.612377288499395	26.405460465453245	23.674091567644894
56-57	23.27463336748531	26.74074504779606	26.374724060703635	23.60989752401499
58-59	23.469181920808797	26.71254438890192	26.552988301314507	23.265285388974778
60-61	23.55374485812337	26.632775572106038	26.550168702475986	23.263310867294603
62-63	23.36584090344594	26.625122284377785	26.535369093621107	23.47366771855517
64-65	23.22559394178643	26.625398419809187	26.663911944793984	23.485095693610404
66-67	23.339213791871867	26.754740768820717	26.355036226440514	23.551009212866898
68-69	23.40493562423949	26.582612830184843	26.48606155407854	23.526389991497123
70-71	23.18390215511445	26.32062283398446	26.647201099546553	23.84827391135454
72-73	23.361019697374598	26.446709553326276	26.49635972283067	23.695911026468462
74-75	23.261027992510236	26.425851447318756	26.519567060866628	23.793553499304384
76-77	23.322831424989108	26.327785188967518	26.56109063008777	23.788292755955613
78-79	23.249247151513256	26.472022801528034	26.619912466534686	23.658817580424028
80-81	23.323128755201875	26.49006356941546	26.589409161075135	23.597398514307528
82-83	23.32730309194456	26.474332531944818	26.616943686171517	23.581420689939105
84-85	23.544927538944	26.38241691730635	26.371055090025102	23.70160045372455
86-87	23.25348439508634	26.342101716957604	26.659258673020858	23.7451552149352
88-89	23.224349396614432	26.312414737252393	26.69558415491668	23.767651711216494
90-91	23.421485362991866	26.380646422078048	26.598364282294302	23.599503932635784
92-93	23.297708327679825	26.25127880665299	26.766517743814106	23.68449512185308
94-95	23.150799508126617	26.39491005424964	26.752466017278664	23.701824420345076
96-97	23.219588486717885	26.299020508827454	26.68822371565014	23.793167288804526
98-99	23.144771161497907	26.14723346931432	26.684473986969724	24.02352138221805
100-101	23.131587816632646	26.292376164120835	26.693833329701427	23.882202689545096
102-103	22.964300643356538	26.29640814099276	26.872938587503082	23.86635262814762
104-105	22.997635363698013	26.277941615446693	26.743062060306848	23.981360960548447
106-107	22.855982030758994	26.273036258673756	26.875598354147577	23.99538335641967
108-109	22.858389545888468	26.18848463321483	26.89650949877326	24.056616322123443
110-111	22.68527016784944	26.550699162096432	26.768019579292808	23.99601109076132
112-113	22.749748899018773	26.585638868533767	26.68733576846257	23.977276463984886
114-115	22.782970835323592	26.581716827899438	26.69799746770766	23.937314869069308
116-117	22.7767048070337	26.465674471726775	26.815851485164398	23.94176923607513
118-119	22.671050100569545	26.244059365639906	26.829818311797805	24.255072221992748
120-121	22.84695282527036	26.45354044407609	26.76745491732057	23.932051813332976
122-123	22.7438282472535	26.39937042601049	26.806328999197127	24.050472327538888
124-125	22.69934071901015	26.540329553562653	26.593826337402877	24.16650339002432
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1287.0
1	898.0
2	443.0
3	331.0
4	260.5
5	223.5
6	226.5
7	269.0
8	318.5
9	377.0
10	416.5
11	447.5
12	493.0
13	549.5
14	656.0
15	777.0
16	908.5
17	1062.0
18	1244.5
19	1492.5
20	1848.0
21	2343.5
22	3036.5
23	4069.5
24	5426.5
25	7265.5
26	10272.5
27	14118.5
28	18129.5
29	22974.0
30	28825.0
31	35980.0
32	43656.0
33	51395.5
34	60675.0
35	69390.0
36	76722.0
37	84843.5
38	92674.0
39	99695.5
40	107665.0
41	115096.5
42	120881.0
43	128097.5
44	135223.0
45	140566.0
46	146284.5
47	152495.5
48	157456.0
49	160938.5
50	163164.5
51	158960.0
52	152871.0
53	147444.5
54	139971.5
55	132834.5
56	122667.0
57	111141.5
58	100495.5
59	87085.5
60	71805.5
61	57731.0
62	45280.5
63	34952.0
64	26817.0
65	20018.5
66	14586.0
67	11265.5
68	8576.5
69	6276.0
70	4770.5
71	3371.0
72	2422.0
73	1776.0
74	1270.5
75	886.5
76	612.0
77	414.0
78	263.5
79	178.5
80	138.0
81	102.5
82	72.5
83	58.0
84	37.5
85	28.0
86	25.5
87	21.0
88	16.5
89	16.5
90	19.0
91	18.0
92	16.0
93	12.0
94	8.0
95	7.0
96	4.0
97	5.0
98	11.0
99	12.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0360633185257187
2	0.019248027678663803
3	0.024567968662072268
4	0.02221543194579114
5	0.015425155514706964
6	0.00534667435518439
7	0.005747674931823219
8	0.006255608995565736
9	0.003074337754231024
10-11	0.0053867744128482725
12-13	0.008327445308199686
14-15	0.003047604382455102
16-17	0.0027134372352560776
18-19	0.0
20-21	0.003969905708724409
22-23	0.0010693348710368777
24-25	0.0018178692807626926
26-27	0.0014302353900118242
28-29	0.0028738374659116094
30-31	0.001483702133563668
32-33	0.0
34-35	0.0068437431746360185
36-37	0.008046744904552506
38-39	0.001537168877115512
40-41	0.0010693348710368777
42-43	0.002539670318712585
44-45	0.0011629016722526048
46-47	0.021640664452608815
48-49	0.003101071126006946
50-51	0.01070671539625674
52-53	0.0015772689347793949
54-55	2.5396703187125847E-4
56-57	2.8070040364718045E-4
58-59	0.007097710206507277
60-61	9.089346403813463E-4
62-63	0.010319081505505872
64-65	0.010653248652704896
66-67	1.3366685887960972E-4
68-69	0.003836238849844799
70-71	0.0051328073809770135
72-73	0.012684984907674965
74-75	0.0018980693960904585
76-77	0.0014168687041238633
78-79	4.411006343027122E-4
80-81	0.004411006343027121
82-83	0.0013901353323479413
84-85	0.0012698351593562925
86-87	0.014409287387221928
88-89	0.0
90-91	5.881341790702828E-4
92-93	0.009075979717925502
94-95	0.006669976258092525
96-97	0.004825373605553912
98-99	0.0012431017875803706
100-101	0.01717619136602985
102-103	0.009022512974373658
104-105	0.008594779025958905
106-107	0.018619793441929638
108-109	0.012016650613276914
110-111	0.020678263068675626
112-113	0.003101071126006946
114-115	0.014796921277972797
116-117	0.006656609572204565
118-119	0.0052531075539686625
120-121	0.007832877930345131
122-123	0.008982412916709774
124-125	0.05615344741532405
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	3740643.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.97969513989465
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.73379792765793	34.85273961371361
2	14.105562426091147	14.099834196655767
3	6.000793087445938	8.997534273244055
4	3.2410903086898815	6.479548221967492
5	1.9184779201550195	4.7942470790983505
6	1.2365795239670576	3.7082320574465752
7	0.8233020249489301	2.880386895050383
8	0.588806266728734	2.3542686166049296
9	0.4303809290613682	1.9359276865660628
>10	1.830882202157482	15.00184552936697
>50	0.06481930726904728	2.203073786455839
>100	0.02442809164283291	2.1113506029898135
>500	7.559889341708314E-4	0.27720032320352467
>1k	3.239952505336874E-4	0.30381111763662216
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	5.3466743551843895E-5	0.0	0.0	0.0	0.0
3	5.3466743551843895E-5	0.0	0.0	0.0	0.0
4	5.3466743551843895E-5	0.0	0.0	2.6733371775921947E-5	0.0
5	1.8713360243145363E-4	0.0	0.0	2.6733371775921947E-5	0.0
6	2.673337177592195E-4	0.0	0.0	2.6733371775921947E-5	0.0
7	4.2773394841475116E-4	0.0	0.0	5.3466743551843895E-5	0.0
8	4.2773394841475116E-4	0.0	0.0	5.3466743551843895E-5	0.0
9	4.2773394841475116E-4	0.0	0.0	1.8713360243145363E-4	0.0
10-11	4.2773394841475116E-4	0.0	0.0	1.8713360243145363E-4	0.0
12-13	5.34667435518439E-4	0.0	0.0	1.8713360243145363E-4	0.0
14-15	9.089346403813462E-4	0.0	0.0	2.4060034598329753E-4	0.0
16-17	0.0010158681274850339	0.0	0.0	2.539670318712585E-4	0.0
18-19	0.0014436020758997851	0.0	0.0	2.673337177592195E-4	0.0
20-21	0.0017911359089867705	0.0	0.0	4.0100057663882923E-4	0.0
22-23	0.002005002883194146	0.0	0.0	6.683342943980487E-4	0.0
24-25	0.002432736831608897	0.0	0.0	0.001069334871036878	0.0
26-27	0.0024728368892727804	0.0	0.0	0.0017644025372108487	0.0
28-29	0.0032481046707745166	0.0	0.0	0.0033951382155420875	0.0
30-31	0.003675838619189268	0.0	0.0	0.010198781332514222	0.0
32-33	0.0037025719909651897	0.0	0.0	0.02543680324478973	0.0
34-35	0.004170405997043824	2.6733371775921947E-5	0.0	0.044711564295229454	0.0
36-37	0.004678340060786341	2.6733371775921947E-5	0.0	0.07827531255989946	0.0
38-39	0.00507934063742517	2.6733371775921947E-5	0.0	0.13184898959884706	0.0
40-41	0.005092707323313131	2.6733371775921947E-5	0.0	0.19186540923579182	0.0
42-43	0.0055204412717278824	4.010005766388292E-5	0.0	0.25034465999562105	0.0
44-45	0.00617540888023797	5.3466743551843895E-5	0.0	0.316803822230563	0.0
46-47	0.007298210494826691	5.3466743551843895E-5	0.0	0.3956806356554208	0.0
48-49	0.007565544212585911	1.0693348710368779E-4	0.0	0.47843378798778713	0.0
50-51	0.007565544212585911	2.1386697420737558E-4	0.0	0.5659187471244918	0.0
52-53	0.007578910898473872	2.1386697420737558E-4	0.0	0.6557696096633654	0.0
54-55	0.0076591110138016375	2.1386697420737558E-4	0.0	0.7570757220082216	0.0
56-57	0.00810021164810435	2.2723366009533654E-4	0.0	0.8719356538434702	0.0
58-59	0.00810021164810435	2.4060034598329753E-4	0.0	1.0000152380219123	0.0
60-61	0.008126945019880271	2.4060034598329753E-4	0.0	1.135085598919758	0.0
62-63	0.00823387850698396	2.4060034598329753E-4	0.0	1.2715460951499515	0.0
64-65	0.008287345250535804	2.4060034598329753E-4	0.0	1.4185395398598581	0.0
66-67	0.008902212801382009	2.940670895351414E-4	0.0	1.5832705767430895	0.0
68-69	0.008982412916709774	2.940670895351414E-4	0.0	1.7722086817694178	0.0
70-71	0.009263113320356954	3.609005189749463E-4	0.0	1.9776947439250419	1.3366685887960974E-5
72-73	0.0096240138393319	4.2773394841475116E-4	0.0	2.186883378071631	4.010005766388292E-5
74-75	0.009837880813539276	4.2773394841475116E-4	0.0	2.4006968855354547	5.3466743551843895E-5
76-77	0.009864614185315198	4.2773394841475116E-4	0.0	2.639252128577894	5.3466743551843895E-5
78-79	0.00987798087120316	4.2773394841475116E-4	0.0	2.9094864171748016	5.3466743551843895E-5
80-81	0.011174549402335374	4.2773394841475116E-4	0.0	3.198688033046725	5.3466743551843895E-5
82-83	0.011281482889439061	4.2773394841475116E-4	0.0	3.5053465406883255	5.3466743551843895E-5
84-85	0.01153544992131032	4.2773394841475116E-4	0.0	3.8160551541539784	5.3466743551843895E-5
86-87	0.01178941695318158	4.2773394841475116E-4	0.0	4.1521471041208695	5.3466743551843895E-5
88-89	0.011842883696733423	4.2773394841475116E-4	0.0	4.52428900592759	5.3466743551843895E-5
90-91	0.012965685311322145	4.8120069196659507E-4	0.0	4.925476716168852	5.3466743551843895E-5
92-93	0.013219652343193403	4.94567377854556E-4	0.0	5.34345298388539	5.3466743551843895E-5
94-95	0.01329985245852117	5.07934063742517E-4	0.0	5.772443400773611	5.3466743551843895E-5
96-97	0.01331321914440913	5.213007496304781E-4	0.0	6.216818873118873	5.3466743551843895E-5
98-99	0.013887986637591452	5.34667435518439E-4	0.0	6.695920995401059	5.3466743551843895E-5
100-101	0.014583054303765422	5.34667435518439E-4	0.0	7.223477354027102	5.3466743551843895E-5
102-103	0.015184555168723666	5.480341214063999E-4	0.0	7.768477237736935	5.3466743551843895E-5
104-105	0.017243024795469655	5.614008072943609E-4	0.0	8.320494631537947	5.3466743551843895E-5
106-107	0.017777692230988094	5.614008072943609E-4	0.0	8.872137758134096	5.3466743551843895E-5
108-109	0.018031659262859354	5.881341790702828E-4	0.0	9.45666293201463	6.683342943980487E-5
110-111	0.01840592646772226	6.148675508462048E-4	0.0	10.076970724017235	8.020011532776584E-5
112-113	0.018579693384265752	6.148675508462048E-4	0.0	10.719186514190207	8.020011532776584E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	10010	0.0	30.973612	1
TCAACGC	10510	0.0	28.192497	4
CAACGCA	10715	0.0	27.7638	5
ATCAACG	10665	0.0	27.727343	3
GTACATG	15950	0.0	27.721474	1
AACGCAG	11165	0.0	26.751734	6
TACATGG	16250	0.0	26.070597	2
TATCAAC	11340	0.0	26.025133	2
ACATGGG	16340	0.0	25.671415	3
CATGGGG	9975	0.0	24.097687	4
ACGCAGA	12660	0.0	23.216682	7
CGCAGAG	12760	0.0	22.801588	8
GGTATCA	7355	0.0	21.764957	1
GTGTAGC	3090	0.0	20.414352	1
GTATAGC	1980	0.0	19.836588	1
GCAGAGT	14250	0.0	19.16482	9
ATGGGGA	6220	0.0	19.035479	5
CGTATAC	355	4.0136E-8	18.436438	3
TAGCCCT	3720	0.0	18.073505	4
GTATAGG	1260	0.0	17.947388	1
>>END_MODULE