FastQCFastQC Report
Mon 15 Aug 2016
SRR2920951_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920951_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3740643
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39600.1058641522326509No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA128550.034.077471
TCAACGC148000.028.7445014
GGTATCA90350.028.5635261
ATCAACG150050.028.510243
TATCAAC152400.028.0668562
CAACGCA151450.027.8925
AACGCAG155800.027.185626
ACGCAGA173100.024.2290367
CGCAGAG175050.023.6535878
ACCGTAT3307.403287E-1021.6330248
GTACCGT4800.021.0685376
GAGTACT77400.020.98740412-13
GTACATG163000.020.73231
GCAGAGT190900.020.7238989
TACATGG160900.020.0676982
AGTACTT86450.020.06367912-13
ACATGGG163350.019.550513
CATGGGG93800.019.4373424
AGAGTAC174550.019.19056710-11
GTACACT30000.018.8677734
Produced by FastQC (version 0.11.5)