##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2920951_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3740643 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.833867332434558 31.0 30.0 34.0 26.0 34.0 2 29.944005081479308 31.0 30.0 34.0 26.0 34.0 3 30.11088334278358 31.0 30.0 34.0 26.0 34.0 4 33.64788540365921 35.0 35.0 37.0 28.0 37.0 5 33.140178038909355 35.0 33.0 37.0 26.0 37.0 6 32.92476346980987 35.0 33.0 37.0 26.0 37.0 7 32.774811977512954 35.0 33.0 37.0 25.0 37.0 8 32.712830120383046 35.0 33.0 37.0 25.0 37.0 9 34.02713490702 38.0 34.0 39.0 25.0 39.0 10-11 33.9303690568707 37.5 33.5 39.0 25.0 39.0 12-13 33.81034062860316 37.0 33.0 39.0 24.0 39.0 14-15 34.70756966115184 38.0 33.0 41.0 24.0 41.0 16-17 34.69793762730097 38.0 33.0 41.0 24.0 41.0 18-19 34.73994604670908 38.5 33.5 41.0 24.0 41.0 20-21 34.71120085504016 39.0 34.0 41.0 23.5 41.0 22-23 34.66983256621923 39.0 34.0 41.0 23.0 41.0 24-25 34.63841296803785 39.0 34.0 41.0 23.0 41.0 26-27 34.49511313963936 39.0 33.5 41.0 22.5 41.0 28-29 34.37956402147973 39.0 33.0 41.0 22.0 41.0 30-31 34.22122172578351 38.0 33.0 40.5 20.0 41.0 32-33 34.0917335602462 38.0 33.0 40.0 19.0 41.0 34-35 33.970589281040716 38.0 33.0 40.0 18.5 41.0 36-37 33.840112916415706 38.0 33.0 40.0 18.0 41.0 38-39 33.709448482520244 38.0 33.0 40.0 16.0 41.0 40-41 33.531589756092735 38.0 33.0 40.0 15.5 41.0 42-43 33.38905637346306 38.0 32.0 40.0 15.0 41.0 44-45 33.180919430162135 38.0 32.0 40.0 11.0 41.0 46-47 33.04799468968303 38.0 32.0 40.0 9.0 41.0 48-49 32.853868706529866 38.0 32.0 40.0 8.0 41.0 50-51 32.666289325124055 38.0 31.5 40.0 7.5 41.0 52-53 32.43753560550954 37.5 31.0 40.0 4.0 41.0 54-55 32.26606588760275 37.0 31.0 40.0 2.0 41.0 56-57 32.143367864829656 37.0 31.0 40.0 2.0 41.0 58-59 32.08911917015337 37.0 31.0 40.0 2.0 41.0 60-61 31.830436237833975 36.5 31.0 40.0 2.0 41.0 62-63 31.58162179603881 36.0 30.5 40.0 2.0 41.0 64-65 31.316359647258505 36.0 30.0 39.0 2.0 41.0 66-67 31.010193835658733 35.0 30.0 39.0 2.0 41.0 68-69 30.697911428596633 35.0 29.5 39.0 2.0 40.5 70-71 30.34765533626171 35.0 29.0 38.0 2.0 40.0 72-73 30.056360764713446 35.0 29.0 37.0 2.0 39.5 74-75 29.7782545674634 35.0 29.0 37.0 2.0 39.0 76-77 29.431046079510928 34.0 29.0 36.0 2.0 39.0 78-79 29.094419595775378 34.0 29.0 36.0 2.0 38.5 80-81 28.822488406404993 34.0 29.0 35.5 2.0 37.0 82-83 28.55612043170118 34.0 28.0 35.0 2.0 37.0 84-85 28.342243566146248 34.0 28.0 35.0 2.0 36.0 86-87 28.10531344477407 34.0 27.5 35.0 2.0 36.0 88-89 27.968141974521494 34.0 27.0 35.0 2.0 36.0 90-91 27.773433738531047 34.0 27.0 35.0 2.0 35.0 92-93 27.89920008404972 34.0 27.5 35.0 2.0 35.0 94-95 27.839922040141225 34.0 27.5 35.0 2.0 35.0 96-97 27.772324838269782 34.0 27.0 35.0 2.0 35.0 98-99 27.721568724949158 34.0 27.0 35.0 2.0 35.0 100-101 27.584846241675564 34.0 27.0 35.0 2.0 35.0 102-103 26.295293349298504 31.5 24.5 34.0 2.0 35.0 104-105 27.345813540613204 33.5 26.5 35.0 2.0 35.0 106-107 27.5357935788045 34.0 27.0 35.0 2.0 35.0 108-109 27.519383833207286 34.0 27.0 35.0 2.0 35.0 110-111 27.46449327027466 34.0 27.0 35.0 2.0 35.0 112-113 27.36302515369684 34.0 27.0 35.0 2.0 35.0 114-115 27.2731748258254 34.0 27.0 35.0 2.0 35.0 116-117 27.144117067573674 34.0 27.0 35.0 2.0 35.0 118-119 27.019274226383004 34.0 26.5 35.0 2.0 35.0 120-121 26.88770246184947 34.0 26.0 35.0 2.0 35.0 122-123 26.65551577629835 34.0 25.0 35.0 2.0 35.0 124-125 25.557611351845125 32.5 22.0 34.5 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 135890.0 3 41916.0 4 20249.0 5 15671.0 6 16813.0 7 19618.0 8 22148.0 9 24036.0 10 25088.0 11 26390.0 12 27438.0 13 29535.0 14 31943.0 15 27350.0 16 21475.0 17 19269.0 18 17625.0 19 16894.0 20 16753.0 21 17554.0 22 20087.0 23 25718.0 24 34697.0 25 45704.0 26 55955.0 27 65896.0 28 77656.0 29 90869.0 30 107729.0 31 128299.0 32 155990.0 33 193929.0 34 248176.0 35 336671.0 36 532632.0 37 781164.0 38 265253.0 39 563.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.17235782708637 19.831803487929832 13.892073280655795 25.103765404328005 2 15.653800234077716 20.58739712316455 38.65260418142574 25.106198461331996 3 18.663303167529683 24.50584125485323 29.693127933894196 27.137727643722886 4 13.11804465553312 16.889053891138488 36.2227434009212 33.77015805240719 5 13.617441576606096 37.84295276208475 34.53229600241499 14.00730965889417 6 32.96271730549342 36.6880994640063 16.878682132928258 13.470501097572027 7 28.28223660324958 32.08595179902161 21.04396241921366 18.58784917851515 8 25.920650915845034 36.396247587366155 19.341255711248067 18.341845785540748 9 26.26943402825781 15.765666151198015 19.16503170493195 38.79986811561223 10-11 25.07569539693641 26.271032236940528 27.74603460670414 20.907237759418926 12-13 25.87393944936196 23.450186591134457 27.870951905571907 22.804922053931676 14-15 22.93675943233508 24.951464488852878 25.43699341895907 26.674782659852976 16-17 23.001809890009234 27.906391178273832 26.13133503290496 22.960463898811973 18-19 22.845081832284702 27.11697434703532 27.705233947223125 22.332709873456857 20-21 23.769726438307302 26.258965104345766 27.548493520203603 22.42281493714333 22-23 23.30398444162011 26.257627240106952 27.224958760292544 23.213429557980394 24-25 22.945069125853358 26.383833992063156 27.54851698218047 23.122579899903016 26-27 23.040205956491477 26.554012373326092 27.423185453627568 22.982596216554864 28-29 22.979090471054 26.658004944615072 27.441530702230533 22.921373882100397 30-31 23.43281542639614 26.402878544298584 27.370491177291672 22.793814852013604 32-33 23.120138300233442 26.51306659108108 27.35585035123232 23.01094475745316 34-35 23.12206602587493 26.605376644426105 27.422894869177966 22.849662460521003 36-37 23.25687119636469 26.41034435037937 27.376934869127865 22.955849584128075 38-39 23.217636367453203 26.549325501793795 27.32139927440415 22.911638856348844 40-41 23.38894019802817 26.473474497277778 27.21236621719504 22.925219087499013 42-43 23.347043234324317 26.36680046745592 27.239416054490174 23.04674024372959 44-45 23.341402118029244 26.460015927203372 27.072501934743393 23.12608002002399 46-47 23.250035034869054 26.42855347176831 27.00700322962655 23.314408263736077 48-49 23.2753953510823 26.55066768874771 26.971841560323572 23.20209539984642 50-51 23.309490661542224 26.609614320417002 26.809254748379125 23.27164026966165 52-53 23.31894368596393 26.67926462778119 26.601540208381135 23.40025147787374 54-55 23.25728197789861 26.44710933804139 26.701414637577177 23.594194046482823 56-57 23.215982295730925 26.60108678241863 26.61965797452872 23.56327294732172 58-59 23.331348235915144 26.56258231312964 26.903467306461692 23.202602144493518 60-61 23.4746073221416 26.538631263923918 26.804111717619623 23.182649696314858 62-63 23.251382654415632 26.61856658640328 26.722024108437818 23.408026650743267 64-65 23.143721615275844 26.53873587968207 26.85931393381219 23.458228571229895 66-67 23.22725511271385 26.69463908862929 26.541195493425125 23.536910305231736 68-69 23.207006790987602 26.50936017103822 26.674648985229364 23.60898405274481 70-71 23.087592610248098 26.26133596772302 26.77730711120745 23.873764310821436 72-73 23.265755372229275 26.401659109839805 26.582704697249763 23.74988082068116 74-75 23.158812768855626 26.375828176270083 26.581821021137298 23.883538033736997 76-77 23.17147209149321 26.304859447056057 26.62056995546709 23.903098505983646 78-79 23.104288104806226 26.47326594860639 26.651553126811333 23.77089281977605 80-81 23.14306934631308 26.491544379888882 26.569211608770555 23.796174665027486 82-83 23.10328085233671 26.513451378246277 26.57077606825904 23.81249170115798 84-85 23.261833113089946 26.384953000185575 26.412949134740288 23.9402647519842 86-87 23.10012740161675 26.395989041963375 26.55631076581101 23.947572790608866 88-89 22.98358084523314 26.379422270923076 26.583493397639852 24.05350348620394 90-91 23.128752593545823 26.415972057029997 26.532690811898014 23.92258453752617 92-93 22.992837021207354 26.34654655321172 26.643545504028467 24.01707092155246 94-95 22.852349226586348 26.427448751050864 26.655175806944996 24.065026215417788 96-97 22.862021467988594 26.35822682205773 26.574861284557873 24.204890425395806 98-99 22.860938096438232 26.24665827461011 26.495425036718036 24.396978592233626 100-101 22.843253129963685 26.354347721450612 26.510097174609452 24.292301973976244 102-103 22.652410978831732 26.379186321385404 26.58129566045203 24.38710703933083 104-105 22.68789591418632 26.315221595624333 26.463589766353223 24.533292723836123 106-107 22.538705046204615 26.342866944105136 26.62492210996729 24.493505899722958 108-109 22.56818551293816 26.238360788289878 26.565919168843173 24.62753452992879 110-111 22.32939303126059 26.62526931785289 26.391979726039484 24.653357924847032 112-113 22.360502616770756 26.64972597033346 26.324267280125213 24.665504132770568 114-115 22.40883671229148 26.67672629135541 26.282425010364214 24.632011985988893 116-117 22.26987993491612 26.564729011671222 26.414986886033553 24.750404167379102 118-119 22.21777370563986 26.421675347289526 26.351601185245233 25.008949761825384 120-121 22.27980798369377 26.64728199023022 26.277897498711038 24.79501252736497 122-123 22.147653787816104 26.607234112461036 26.24265586842781 25.002456231295046 124-125 22.08492667432389 26.779350969554315 26.032698306498503 25.10302404962329 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 92.0 1 96.5 2 199.5 3 373.5 4 345.0 5 240.5 6 289.5 7 568.0 8 900.0 9 954.0 10 807.5 11 639.0 12 578.5 13 661.0 14 776.0 15 906.5 16 1108.5 17 1270.0 18 1534.0 19 1864.5 20 2202.0 21 2692.0 22 3357.5 23 4373.5 24 5770.5 25 7588.5 26 10658.5 27 14670.5 28 18753.0 29 23706.5 30 29787.5 31 36965.0 32 44403.5 33 51960.0 34 61209.0 35 70140.0 36 77786.0 37 85843.5 38 92960.0 39 99658.0 40 107582.5 41 114651.0 42 120696.5 43 128366.5 44 135461.0 45 141607.5 46 147982.0 47 153659.5 48 158537.0 49 162082.0 50 164551.5 51 160120.5 52 153060.0 53 147335.5 54 140325.0 55 132914.0 56 122303.0 57 110515.0 58 99240.0 59 85578.0 60 69372.5 61 55001.5 62 43502.0 63 33494.0 64 25332.0 65 18768.5 66 13450.5 67 10214.0 68 7632.5 69 5339.0 70 4032.0 71 2884.0 72 2025.0 73 1428.0 74 992.0 75 678.5 76 433.0 77 271.0 78 167.5 79 108.5 80 80.5 81 62.0 82 35.5 83 24.5 84 14.5 85 7.5 86 5.5 87 7.5 88 7.5 89 5.0 90 5.0 91 7.5 92 6.5 93 5.0 94 5.0 95 7.0 96 9.0 97 6.5 98 2.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.051354807181546065 2 1.3366685887960972E-4 3 0.002512936946936663 4 0.12476464607822772 5 0.017644025372108484 6 0.003047604382455102 7 0.0059080751624787506 8 0.011655750094301968 9 0.026840305263025634 10-11 0.021881264798592114 12-13 0.020892130042883 14-15 0.029754242786601125 16-17 0.03917775633761362 18-19 0.041770893399878044 20-21 0.023124366586172486 22-23 0.024648168777400035 24-25 0.023258033445052093 26-27 0.022028298343359686 28-29 0.02184116474092823 30-31 0.026065037481523896 32-33 0.025476903302453613 34-35 0.03086367771530189 36-37 0.032360746534753515 38-39 0.03297561408559972 40-41 0.03546181766076047 42-43 0.0334969148352302 44-45 0.030181976735015877 46-47 0.04039412475341806 48-49 0.03244094665008128 50-51 0.02458133534796023 52-53 0.03071664417053432 54-55 0.033149381002143215 56-57 0.026198704340403507 58-59 0.029727509414825208 60-61 0.042051593803525224 62-63 0.05107410677789888 64-65 0.04320112878988987 66-67 0.03967232371546817 68-69 0.030155243363239957 70-71 0.03698561985198801 72-73 0.040888692131272615 74-75 0.025396703187125853 76-77 0.022041665029247644 78-79 0.03129141166371664 80-81 0.02594473730853225 82-83 0.03786782112059344 84-85 0.02248276566355036 86-87 0.034405849475611545 88-89 0.033697415123549616 90-91 0.03508755045589756 92-93 0.028537874370796676 94-95 0.022963966355516955 96-97 0.01878019367258517 98-99 0.026613071602930298 100-101 0.026278904455731274 102-103 0.028952241633323467 104-105 0.02565067021899711 106-107 0.0158929895207856 108-109 0.01868662687136944 110-111 0.023271400130940055 112-113 0.014355820643670086 114-115 0.016293990097424427 116-117 0.014609787675541344 118-119 0.008554678968295022 120-121 0.006188775566125931 122-123 0.004183772682931785 124-125 0.06842406506047223 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3740643.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.371337339764544 #Duplication Level Percentage of deduplicated Percentage of total 1 68.34189601740425 33.05788906696972 2 14.574490100442326 14.099751544071093 3 6.420622136060311 9.317222378235988 4 3.377949530640626 6.535837450532682 5 1.9843799037958707 4.799355486837978 6 1.3015936128465442 3.7775894235769845 7 0.8887838649506622 3.0094164907567755 8 0.6284308833009999 2.4318433800703096 9 0.4538262819064384 1.975696575817267 >10 1.9243337227559358 15.351861345544693 >50 0.07299557077084153 2.3793030706146014 >100 0.02952968531560236 2.4989105253593955 >500 7.234746492269237E-4 0.2725067689398668 >1k 4.4521516032167407E-4 0.4928164926726457 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3960 0.1058641522326509 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.6040023065553167E-4 0.0 0.0 2.6733371775921947E-5 0.0 3 1.6040023065553167E-4 0.0 0.0 2.6733371775921947E-5 0.0 4 1.6040023065553167E-4 2.6733371775921947E-5 0.0 1.3366685887960974E-4 0.0 5 2.673337177592195E-4 2.6733371775921947E-5 0.0 1.3366685887960974E-4 0.0 6 3.475338330869853E-4 2.6733371775921947E-5 0.0 1.3366685887960974E-4 0.0 7 7.218010379498926E-4 2.6733371775921947E-5 0.0 1.6040023065553167E-4 0.0 8 7.752677815017364E-4 2.6733371775921947E-5 0.0 1.6040023065553167E-4 0.0 9 7.752677815017364E-4 2.6733371775921947E-5 0.0 2.4060034598329753E-4 0.0 10-11 8.287345250535804E-4 2.6733371775921947E-5 0.0 2.4060034598329753E-4 0.0 12-13 9.757680698211512E-4 2.6733371775921947E-5 0.0 2.673337177592195E-4 0.0 14-15 0.0011762683581405657 2.6733371775921947E-5 0.0 3.2080046131106334E-4 0.0 16-17 0.0012564684734683315 2.6733371775921947E-5 0.0 3.3416714719902436E-4 0.0 18-19 0.0016708357359951217 2.6733371775921947E-5 0.0 3.609005189749463E-4 0.0 20-21 0.002031736254970068 2.6733371775921947E-5 0.0 4.94567377854556E-4 0.0 22-23 0.0021787697997376386 2.6733371775921947E-5 0.0 7.752677815017364E-4 0.0 24-25 0.002967404267127336 2.6733371775921947E-5 0.0 0.0011896350440285266 0.0 26-27 0.003034237696567141 2.6733371775921947E-5 0.0 0.0018579693384265755 0.0 28-29 0.0037560387345170333 2.6733371775921947E-5 0.0 0.003475338330869853 0.0 30-31 0.00411693925349198 2.6733371775921947E-5 0.0 0.010238881390178105 0.0 32-33 0.004197139368819746 2.6733371775921947E-5 0.0 0.02515610284114255 0.0 34-35 0.004638240003122457 8.020011532776584E-5 0.0 0.044190263545598976 0.0 36-37 0.005159540752752936 8.020011532776584E-5 0.0 0.07743321134895792 0.0 38-39 0.005614008072943609 8.020011532776584E-5 0.0 0.13005785368986028 0.0 40-41 0.005640741444719531 9.356680121572681E-5 0.0 0.1893257389170792 0.0 42-43 0.0061887755661259305 1.0693348710368779E-4 0.0 0.2467490214917596 0.0 44-45 0.00700414340529155 1.0693348710368779E-4 0.0 0.3123259824580961 0.0 46-47 0.007712577757353482 1.0693348710368779E-4 0.0 0.3901869277554688 0.0 48-49 0.008046744904552506 1.6040023065553167E-4 0.0 0.47172371167203075 0.0 50-51 0.008046744904552506 1.8713360243145363E-4 0.0 0.5584066696554577 0.0 52-53 0.008046744904552506 1.8713360243145363E-4 0.0 0.6476827647011489 0.0 54-55 0.008126945019880273 1.8713360243145363E-4 0.0 0.7484675762963747 0.0 56-57 0.008527945596519102 1.8713360243145363E-4 0.0 0.8625388736642337 0.0 58-59 0.008527945596519102 2.0050028831941462E-4 0.0 0.9898699234329499 0.0 60-61 0.008594779025958905 2.1386697420737558E-4 0.0 1.1227882479028337 0.0 62-63 0.008661612455398711 2.1386697420737558E-4 0.0 1.2585135764091895 0.0 64-65 0.008715079198950555 2.1386697420737558E-4 0.0 1.4045178863633874 0.0 66-67 0.009196279890917149 2.1386697420737558E-4 0.0 1.5685137555227806 0.0 68-69 0.009410146865124525 2.1386697420737558E-4 0.0 1.7560483585308728 0.0 70-71 0.009704213954659667 2.4060034598329753E-4 0.0 1.9606923194755552 0.0 72-73 0.01003838110185869 2.673337177592195E-4 0.0 2.168383884802693 0.0 74-75 0.01030571481961791 2.673337177592195E-4 0.0 2.380486456472858 0.0 76-77 0.010399281620833638 2.940670895351414E-4 0.0 2.6177985977277167 0.0 78-79 0.010452748364385481 3.2080046131106334E-4 0.0 2.886950184767699 0.0 80-81 0.011762683581405657 3.2080046131106334E-4 0.0 3.175309699428681 0.0 82-83 0.011896350440285267 3.2080046131106334E-4 0.0 3.479575570296337 0.0 84-85 0.01221715090159633 3.2080046131106334E-4 0.0 3.787356879552526 0.0 86-87 0.012537951362907394 3.2080046131106334E-4 0.0 4.120187358162754 0.0 88-89 0.012591418106459237 3.2080046131106334E-4 0.0 4.4883326208889756 0.0 90-91 0.013527086118616506 3.7426720486290725E-4 0.0 4.8887183299769585 0.0 92-93 0.01374095309282388 3.7426720486290725E-4 0.0 5.303526693138052 0.0 94-95 0.01374095309282388 3.7426720486290725E-4 0.0 5.729857139534566 0.0 96-97 0.01374095309282388 3.7426720486290725E-4 0.0 6.174005378219734 0.0 98-99 0.014262253842454359 3.7426720486290725E-4 0.0 6.653027300386592 0.0 100-101 0.014930588136852408 4.0100057663882923E-4 0.0 7.181840127486103 0.0 102-103 0.015585555745362495 4.0100057663882923E-4 0.0 7.727642012349214 0.0 104-105 0.017911359089867705 4.0100057663882923E-4 0.0 8.280274273701071 0.0 106-107 0.018472759897162065 4.0100057663882923E-4 0.0 8.834243203641726 0.0 108-109 0.018593060070153714 4.143672625267902E-4 0.0 9.420492680001807 0.0 110-111 0.018860393787912932 4.2773394841475116E-4 0.0 10.044195610219955 0.0 112-113 0.019020794018568467 4.2773394841475116E-4 0.0 10.690100605698005 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12855 0.0 34.07747 1 TCAACGC 14800 0.0 28.744501 4 GGTATCA 9035 0.0 28.563526 1 ATCAACG 15005 0.0 28.51024 3 TATCAAC 15240 0.0 28.066856 2 CAACGCA 15145 0.0 27.892 5 AACGCAG 15580 0.0 27.18562 6 ACGCAGA 17310 0.0 24.229036 7 CGCAGAG 17505 0.0 23.653587 8 ACCGTAT 330 7.403287E-10 21.633024 8 GTACCGT 480 0.0 21.068537 6 GAGTACT 7740 0.0 20.987404 12-13 GTACATG 16300 0.0 20.7323 1 GCAGAGT 19090 0.0 20.723898 9 TACATGG 16090 0.0 20.067698 2 AGTACTT 8645 0.0 20.063679 12-13 ACATGGG 16335 0.0 19.55051 3 CATGGGG 9380 0.0 19.437342 4 AGAGTAC 17455 0.0 19.190567 10-11 GTACACT 3000 0.0 18.867773 4 >>END_MODULE