FastQCFastQC Report
Mon 15 Aug 2016
SRR2920950_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920950_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4298306
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA88450.039.4073831
GTACATG168150.034.052271
TCAACGC101950.033.2981344
GGTATCA58150.033.1982271
CAACGCA106200.032.0216645
AACGCAG106800.031.8250776
TACATGG176550.031.7119372
ATCAACG108050.031.47563
ACATGGG176450.031.2573383
CATGGGG124300.030.7606964
TATCAAC112250.030.5101382
ACGCAGA122900.027.3137447
CGCAGAG129300.025.6368798
ATGGGGA60200.022.6553765
ATGGGGG70900.021.756345
GCAGAGT145850.021.6663869
GTATAGA15650.021.3202971
AGAGTAC124250.020.7127410-11
GTATAAT25500.018.2252711
ATAGCGT5700.017.7532836
Produced by FastQC (version 0.11.5)