FastQCFastQC Report
Mon 15 Aug 2016
SRR2920950_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920950_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4298306
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86320.20082330108652105No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70720.16452993342028233No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51410.11960525844367524No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75750.049.9265561
GTATCAA122250.046.208951
TCAACGC158850.035.121874
ATCAACG162600.034.4217223
TATCAAC162900.034.3960842
CAACGCA164150.034.0241435
AACGCAG168900.033.0162626
GTACATG168500.029.87911
ACGCAGA190750.028.791497
TACATGG175050.028.6751612
ACATGGG174050.027.6415633
CGCAGAG198850.027.55668
CATGGGG122600.026.2257864
GCAGAGT222550.024.1689459
AGAGTAC201350.022.97633710-11
GAGTACT122650.022.46237212-13
AGTACTT135800.021.18550712-13
CAGAGTA214150.021.11677410-11
GTACTTT135550.020.50026714-15
ATGGGGG70850.018.8249345
Produced by FastQC (version 0.11.5)