FastQCFastQC Report
Mon 15 Aug 2016
SRR2920949_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920949_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2006086
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25370.12646516649834552No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG53150.030.588654
GTACATG86750.027.6070611
GTATCAA56000.026.5959531
TACATGG91000.026.3746222
ACATGGG89750.025.6802433
GGTATCA41950.023.8583771
TCAACGC61850.023.2048234
ATGGGGG28050.022.7170455
TATCAAC64650.022.6615262
AACGCAG63600.022.6452646
CAACGCA63750.022.4198115
ATCAACG65950.021.8536113
ACGCAGA70000.020.4882837
CGCAGAG69750.020.3905758
GTATTAC5000.020.2554781
TATATAG5100.019.8508765
AATACCG2251.2059697E-418.5274835
ATGGGGA29800.018.3853765
TAACGTC1650.00812004618.046255
GTATAGG4401.3533281E-917.6016861
Produced by FastQC (version 0.11.5)