FastQCFastQC Report
Mon 15 Aug 2016
SRR2920949_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920949_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2006086
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50880.2536282093589208No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43330.21599273411010297No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29970.1493953898287511No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT21520.10727356653702783No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51900.039.882671
GTATCAA76150.035.720461
TCAACGC92750.028.8238534
CAACGCA93150.028.6361585
TATCAAC94000.028.4419772
GTATAGG4200.028.4055251
ATCAACG94500.028.2907833
AACGCAG97500.027.4052566
CATGGGG54550.026.4143664
GTACATG89000.024.798981
ACGCAGA108800.024.5540397
TACATGG89950.023.8972152
CGCAGAG111800.023.8407578
ACATGGG88100.023.4522233
TATAGCG1350.002545618722.0523995
GAGTACT65600.021.58685912-13
ATTATAC7450.021.5793293
TATAGGG4151.8189894E-1221.5220892
AGTACTT70350.021.05967112-13
GTACTTT67900.020.85563914-15
Produced by FastQC (version 0.11.5)