FastQCFastQC Report
Mon 15 Aug 2016
SRR2920948_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920948_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4146671
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA84550.032.77681
GTACATG162550.030.1745011
TACATGG163950.028.9213732
TCAACGC94000.028.7045614
CAACGCA94700.028.4923865
ACATGGG159800.028.4423243
ATCAACG97000.027.9419653
CATGGGG107800.027.8478954
AACGCAG97100.027.830326
TATCAAC103150.027.0845052
GGTATCA63700.024.886821
ACGCAGA110300.024.011777
CGCAGAG113600.023.102178
ATGGGGA65100.021.5014365
GCAGAGT133650.019.057569
GTATAGA9650.018.5276851
GTATAGG8150.018.2814061
TAGACCG4101.081753E-817.4332335
GTAATAC8250.017.3290443
CTATACT11400.017.242084
Produced by FastQC (version 0.11.5)