##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2920948_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4146671 Sequences flagged as poor quality 0 Sequence length 125 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.999437138851864 31.0 30.0 34.0 26.0 34.0 2 30.01864363003479 31.0 30.0 34.0 25.0 34.0 3 30.177720634214772 31.0 31.0 34.0 26.0 34.0 4 33.63526573485092 37.0 35.0 37.0 28.0 37.0 5 33.66251072245664 37.0 35.0 37.0 28.0 37.0 6 33.64467810443607 37.0 35.0 37.0 28.0 37.0 7 33.52164615905144 37.0 35.0 37.0 28.0 37.0 8 33.468623867193706 37.0 35.0 37.0 27.0 37.0 9 34.92224365038847 39.0 35.0 39.0 27.0 39.0 10-11 34.93459801850689 39.0 35.0 39.0 27.0 39.0 12-13 34.89417981315614 39.0 35.0 39.0 27.0 39.0 14-15 36.06902898734913 40.0 36.0 41.0 27.0 41.0 16-17 36.052258908411105 40.0 36.0 41.0 27.0 41.0 18-19 36.02380680791893 40.0 36.0 41.0 27.0 41.0 20-21 36.04501755745754 40.0 36.0 41.0 27.0 41.0 22-23 35.960048916347596 40.0 36.0 41.0 27.0 41.0 24-25 35.959079584563135 40.0 36.0 41.0 27.0 41.0 26-27 35.90010950953186 40.0 36.0 41.0 27.0 41.0 28-29 35.80064345591922 40.0 36.0 41.0 27.0 41.0 30-31 35.66625034877375 40.0 35.5 41.0 26.5 41.0 32-33 35.55024452144865 40.0 35.0 41.0 26.0 41.0 34-35 35.49358232181912 40.0 35.0 41.0 25.5 41.0 36-37 35.36580657110245 40.0 35.0 41.0 25.0 41.0 38-39 35.23461072749683 40.0 35.0 41.0 25.0 41.0 40-41 35.10862436397775 39.0 35.0 41.0 24.0 41.0 42-43 34.911885944170635 39.0 34.5 41.0 24.0 41.0 44-45 34.76160973465221 39.0 34.0 41.0 24.0 41.0 46-47 34.56292818986604 39.0 34.0 41.0 23.0 41.0 48-49 34.4384764308526 39.0 34.0 41.0 23.0 41.0 50-51 34.23771707473296 38.5 33.0 40.0 22.0 41.0 52-53 34.03662769484244 38.0 33.0 40.0 21.0 41.0 54-55 33.79225636661312 38.0 33.0 40.0 20.0 41.0 56-57 33.51270754299051 38.0 33.0 40.0 18.5 41.0 58-59 33.23190651006554 37.0 32.0 40.0 18.0 41.0 60-61 32.94767947589766 37.0 32.0 40.0 16.0 41.0 62-63 32.614977412001096 36.0 32.0 39.5 13.0 41.0 64-65 32.30685651212744 36.0 31.0 39.0 12.5 41.0 66-67 31.947557812037658 35.0 31.0 39.0 9.0 40.5 68-69 31.610895462890593 35.0 31.0 38.5 8.0 40.0 70-71 31.26281431538697 35.0 31.0 37.5 7.5 40.0 72-73 31.316231622909076 35.0 31.0 37.0 7.0 39.0 74-75 31.29995639875939 35.0 31.0 37.0 7.0 39.0 76-77 31.034663106863313 35.0 31.0 36.5 6.0 39.0 78-79 30.751709383261897 35.0 31.0 36.0 2.0 38.5 80-81 30.493602096718064 35.0 31.0 35.5 2.0 37.0 82-83 30.27152732878977 35.0 31.0 35.0 2.0 37.0 84-85 30.053293232089068 35.0 31.0 35.0 2.0 36.0 86-87 29.87054265940076 35.0 31.0 35.0 2.0 36.0 88-89 29.693441558300623 35.0 31.0 35.0 2.0 36.0 90-91 29.537767765998314 35.0 30.5 35.0 2.0 35.5 92-93 29.383155306992045 34.5 30.0 35.0 2.0 35.0 94-95 29.280194884040718 34.0 30.0 35.0 2.0 35.0 96-97 29.1942786153037 34.0 30.0 35.0 2.0 35.0 98-99 29.06133751628716 34.0 30.0 35.0 2.0 35.0 100-101 28.936518595277995 34.0 30.0 35.0 2.0 35.0 102-103 27.560088442029766 32.5 26.5 34.5 2.0 35.0 104-105 28.583822661600113 34.0 29.0 35.0 2.0 35.0 106-107 28.72720960982919 34.0 30.0 35.0 2.0 35.0 108-109 28.649522472363977 34.0 29.0 35.0 2.0 35.0 110-111 28.53580438380571 34.0 29.0 35.0 2.0 35.0 112-113 28.4078982875661 34.0 29.0 35.0 2.0 35.0 114-115 28.284292026061387 34.0 29.0 35.0 2.0 35.0 116-117 28.05355054693271 34.0 29.0 35.0 2.0 35.0 118-119 27.839738551720163 34.0 29.0 35.0 2.0 35.0 120-121 27.64544486408495 34.0 29.0 35.0 2.0 35.0 122-123 27.338352861849906 34.0 28.0 35.0 2.0 35.0 124-125 26.02279322376914 33.0 23.0 34.5 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 139002.0 3 21348.0 4 14568.0 5 13116.0 6 15609.0 7 17228.0 8 17906.0 9 19148.0 10 20594.0 11 22290.0 12 22463.0 13 22293.0 14 21934.0 15 20267.0 16 17224.0 17 15966.0 18 15401.0 19 15355.0 20 16044.0 21 17812.0 22 20998.0 23 27426.0 24 36829.0 25 46797.0 26 54422.0 27 59774.0 28 69792.0 29 82341.0 30 97570.0 31 119713.0 32 149491.0 33 190714.0 34 258990.0 35 378109.0 36 680361.0 37 1058465.0 38 328875.0 39 436.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.93603264411642 17.12458517264886 11.175735094116106 25.763647089118614 2 16.714576159186716 19.977799493098086 37.613870583024145 25.693753764691053 3 19.62326398409776 23.556328849554188 28.47043128883666 28.34997587751139 4 13.16830654938728 15.486337530853463 35.34229834755853 36.003057572200724 5 14.347186109328113 37.389884696156045 33.834306378371224 14.428622816144616 6 34.55423008470116 34.849129377135995 16.673893288705322 13.92274724945752 7 29.824383936279723 31.416787217317577 20.37578003835732 18.38304880804538 8 27.00254839401933 35.005886387057046 19.419356255924708 18.57220896299892 9 26.968495478144405 15.308597150489845 18.533741670742476 39.18916570062328 10-11 25.937942395771103 25.6474712914185 27.175932463175172 21.238653849635224 12-13 26.132772215648174 23.050060131819375 27.08355004359949 23.73361760893296 14-15 23.460275799235532 24.45285136345947 24.298956476093075 27.787916361211924 16-17 24.0744269316684 27.20620009632141 24.98896141218624 23.730411559823956 18-19 24.01216447331347 26.51332199761967 26.134431138194223 23.340082390872638 20-21 24.78599910082338 25.913203634063592 25.824408132553017 23.476389132560016 22-23 24.59693050534275 25.711991612308143 25.528480690030026 24.162597192319087 24-25 24.15838738565036 25.82789391548558 25.83093447143434 24.182784227429725 26-27 24.14640288729066 25.91372084511825 25.800242956593745 24.13963331099735 28-29 24.096140433860313 26.189676933583993 25.83573254048888 23.878450092066807 30-31 24.264895477877214 25.98035152291323 25.679848383768512 24.07490461544105 32-33 24.0694354008417 25.977240047438087 25.844659088221423 24.108665463498784 34-35 24.26143324587824 26.08709397667588 25.70036975850159 23.951103018944288 36-37 24.256656323661332 25.926604804924096 25.705646450704844 24.111092420709728 38-39 24.134491250522327 26.068895598129643 25.817683186626844 23.978929964721182 40-41 24.360948092214326 25.949403900692193 25.73255933781921 23.95708866927427 42-43 24.182176593913063 25.885664128331186 25.739737793557133 24.192421484198615 44-45 24.141326619076917 26.035219439456764 25.74602971968253 24.07742422178379 46-47 24.129368421230442 26.037213100551966 25.67518847505874 24.15823000315885 48-49 24.058211193352147 26.051356160679806 25.720512579524257 24.169920066443794 50-51 23.95035192982253 26.16538143748757 25.778364810106545 24.105901822583352 52-53 24.10797852707698 26.082752874002168 25.57756653639136 24.231702062529497 54-55 23.907005467008812 25.91824789299533 25.706669620436905 24.468077019558955 56-57 23.876965008818775 26.178896693076542 25.770446836902778 24.17369146120191 58-59 23.990335004048397 25.916601948225164 25.800657709079378 24.292405338647065 60-61 24.092225181921776 25.847757645492774 25.864372773488387 24.195644399097063 62-63 23.99588852541933 25.927627622699934 25.900455886710755 24.176027965169986 64-65 23.815760200489255 25.73291698256529 25.95294409475077 24.498378722194687 66-67 23.77161171878318 26.069425354146475 25.822191248750016 24.33677167832033 68-69 23.775356303421383 25.7866185321216 26.067667485533093 24.370357678923924 70-71 23.56497782899855 25.65657582057801 26.018579916952856 24.759866433470588 72-73 23.75356584653453 25.766087706101253 26.03649182833748 24.443854619026737 74-75 23.538380294817166 25.837854780967124 26.014136041452335 24.60962888276337 76-77 23.66678137497454 25.67369814114624 26.127190116230242 24.532330367648974 78-79 23.635562716475008 25.760741449557727 26.125102962682956 24.47859287128431 80-81 23.48309163881132 25.705438533943102 26.282175413577242 24.529294413668335 82-83 23.55286738297197 25.665948318247857 26.21452503639904 24.566659262381133 84-85 23.565907485873577 25.65980725730972 26.067947523581847 24.70633773323486 86-87 23.52986030383629 25.556247960238142 26.33907010386271 24.574821632062854 88-89 23.470890214696354 25.44461268391557 26.377308181867537 24.707188919520537 90-91 23.460860338847507 25.57515528489882 26.361422140735097 24.602562235518576 92-93 23.53451440756049 25.51979093386405 26.300471161292606 24.645223497282856 94-95 23.40568883318265 25.43911796456554 26.43170705898379 24.723486143268016 96-97 23.511706528900262 25.309147404508163 26.389349170533936 24.789796896057634 98-99 23.411010163121702 25.229799132280796 26.39516270006576 24.96402800453174 100-101 23.261756069984923 25.34926171432928 26.574785647616057 24.81419656806974 102-103 23.22836369716408 25.301429666974006 26.58854131167068 24.88166532419123 104-105 23.21632959914765 25.39553480436058 26.543017606775578 24.845117989716194 106-107 23.184890581708263 25.43163777258055 26.56393928773298 24.819532357978215 108-109 23.12790873735689 25.40850963461282 26.554914849965705 24.908666778064585 110-111 22.993753296160943 25.623044549315637 26.606448247000408 24.776753907523013 112-113 23.1321945521029 25.68791277843428 26.514235811063696 24.665656858399124 114-115 23.150645676036838 25.77441005330108 26.433207747509147 24.64173652315294 116-117 23.026125665322596 25.700278768524388 26.57015288407716 24.703442682075856 118-119 23.12940141110596 25.6273535977869 26.464456488204966 24.77878850290217 120-121 23.153280602700157 25.775566826153607 26.47884570950774 24.592306861638495 122-123 23.125537134053577 25.855031749350672 26.451976731741905 24.567454384853843 124-125 23.331567965979453 25.8859567862688 26.222293657456223 24.560181590295525 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1542.0 1 1260.0 2 768.5 3 501.5 4 420.0 5 366.0 6 345.5 7 366.5 8 426.5 9 467.5 10 451.5 11 470.5 12 542.0 13 618.5 14 710.0 15 831.5 16 943.0 17 1053.5 18 1280.0 19 1547.0 20 1910.0 21 2529.5 22 3228.5 23 4121.5 24 5383.0 25 7201.5 26 9960.0 27 13349.5 28 16274.5 29 19976.5 30 24884.0 31 30130.0 32 35929.5 33 41951.5 34 48757.0 35 55802.5 36 63818.0 37 72488.0 38 80128.0 39 87217.0 40 96504.0 41 106219.0 42 114831.0 43 125647.0 44 136799.0 45 146917.5 46 157391.0 47 167745.5 48 176717.5 49 182422.0 50 185290.5 51 186427.0 52 184261.5 53 182289.5 54 180593.5 55 174824.5 56 162087.0 57 147079.0 58 133681.0 59 117571.0 60 98843.5 61 79878.5 62 63309.0 63 49317.0 64 37685.0 65 28527.5 66 21330.5 67 16651.5 68 12751.0 69 9133.5 70 6765.0 71 5000.5 72 3731.0 73 2761.0 74 1983.5 75 1400.5 76 970.5 77 634.0 78 391.0 79 258.5 80 198.5 81 150.0 82 98.0 83 69.5 84 56.0 85 47.5 86 41.0 87 37.5 88 26.5 89 18.0 90 19.0 91 15.5 92 14.0 93 13.0 94 10.0 95 9.5 96 9.0 97 7.0 98 6.5 99 6.0 100 10.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1687377657885084 2 0.15012042189988065 3 0.17797409054154525 4 0.1478535432398664 5 0.17508020289046322 6 0.11042592961920537 7 0.11013654085409719 8 0.09795809698912694 9 0.10478284869959542 10-11 0.09033752617461091 12-13 0.07895490141368824 14-15 0.07858110759209014 16-17 0.07841229747911034 18-19 0.07547017836717695 20-21 0.06945330362596888 22-23 0.06037373112069899 24-25 0.06491954630594035 26-27 0.07554252555845399 28-29 0.07092436318193558 30-31 0.06722259856159314 32-33 0.07632628679728871 34-35 0.07284156375077742 36-37 0.0754822362323898 38-39 0.07153931430779051 40-41 0.07596455084090346 42-43 0.075771624997498 44-45 0.06876600530883689 46-47 0.06641472159233275 48-49 0.05827566257366452 50-51 0.06422019012359553 52-53 0.06642677945754559 54-55 0.0678737232830866 56-57 0.07127404127310799 58-59 0.05878209291260387 60-61 0.06889864182617816 62-63 0.06412372720189279 64-65 0.05809479459547188 66-67 0.06325556090656818 68-69 0.06586005979254202 70-71 0.06074752494229709 72-73 0.05591232099194752 74-75 0.0588785558343066 76-77 0.06817516991340765 78-79 0.06203771652007116 80-81 0.06013257381644215 82-83 0.06449752102349089 84-85 0.06637854799669421 86-87 0.06025315246857057 88-89 0.05898707662122218 90-91 0.0625682625894362 92-93 0.05634640413960982 94-95 0.0653656873188155 96-97 0.052270845697669294 98-99 0.05130621648064194 100-101 0.049087569281479046 102-103 0.047375352421255505 104-105 0.045892235000075964 106-107 0.057118107513231695 108-109 0.057118107513231695 110-111 0.06379816484114606 112-113 0.06390668562806164 114-115 0.06652324237924832 116-117 0.07018883340395222 118-119 0.06309880865880124 120-121 0.06399109068455154 122-123 0.0619894850592198 124-125 0.07373384577652772 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 4146671.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.59837732494066 #Duplication Level Percentage of deduplicated Percentage of total 1 69.46220751174843 31.673639479440325 2 14.106374752975084 12.864555973463487 3 5.9505323362117695 8.140038562525348 4 3.141862298787379 5.730552904124495 5 1.9276627839562337 4.394914748904095 6 1.2064544882138337 3.3007420187365537 7 0.8126104961658994 2.5937604015665947 8 0.5855138086074807 2.1358783659077596 9 0.42278939236495405 1.7350659217835602 >10 2.1821745415838887 17.667116180385456 >50 0.13828651417953605 4.364443195771598 >100 0.06183029743719236 4.785414137648 >500 0.0014350312827617953 0.4224441303508391 >1k 2.657464855208071E-4 0.19143397939192408 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.411573042568364E-5 2 4.823146085136728E-5 0.0 0.0 0.0 2.411573042568364E-5 3 4.823146085136728E-5 0.0 0.0 0.0 2.411573042568364E-5 4 4.823146085136728E-5 0.0 0.0 0.0 4.823146085136728E-5 5 1.9292584340546912E-4 0.0 0.0 0.0 4.823146085136728E-5 6 2.8938876510820364E-4 0.0 0.0 0.0 7.234719127705091E-5 7 5.305460693650401E-4 0.0 0.0 0.0 7.234719127705091E-5 8 6.02893260642091E-4 0.0 0.0 0.0 9.646292170273456E-5 9 6.02893260642091E-4 0.0 0.0 0.0 9.646292170273456E-5 10-11 6.511247214934583E-4 0.0 0.0 0.0 9.646292170273456E-5 12-13 7.475876431961928E-4 0.0 0.0 2.411573042568364E-5 9.646292170273456E-5 14-15 8.802241605374529E-4 0.0 0.0 2.411573042568364E-5 2.0498370861831095E-4 16-17 0.0010128606778787128 0.0 0.0 7.234719127705091E-5 3.3762022595957094E-4 18-19 0.0014107702299024929 0.0 0.0 8.440505648989274E-5 3.617359563852546E-4 20-21 0.0017845640515005894 0.0 0.0 1.0852078691557637E-4 3.617359563852546E-4 22-23 0.0018327955123519566 0.0 0.0 1.5675224776694365E-4 4.220252824494637E-4 24-25 0.0026527303468252003 0.0 0.0 3.014466303210455E-4 5.184882041521983E-4 26-27 0.0026768460772508837 0.0 0.0 6.270089910677746E-4 5.305460693650401E-4 28-29 0.003400317990021393 0.0 0.0 0.0026527303468252003 5.305460693650401E-4 30-31 0.0037138224855552803 0.0 0.0 0.00961011857463493 5.305460693650401E-4 32-33 0.003762053946406648 0.0 0.0 0.02729900684187388 5.305460693650401E-4 34-35 0.0041479056332175855 0.0 0.0 0.056069073239714454 5.305460693650401E-4 36-37 0.00483520395034957 0.0 0.0 0.1083278610721709 5.305460693650401E-4 38-39 0.005486328671843028 0.0 0.0 0.19925622264221107 5.546617997907237E-4 40-41 0.005522502267481553 0.0 0.0 0.3097062679918421 6.511247214934583E-4 42-43 0.005884238223866808 0.0 0.0 0.41861290659422945 6.511247214934583E-4 44-45 0.006221858449826379 0.0 0.0 0.5555058503556226 6.511247214934583E-4 46-47 0.007150314071215199 0.0 0.0 0.7251961874959456 6.511247214934583E-4 48-49 0.007524107892813295 0.0 0.0 0.8988776780217191 6.752404519191419E-4 50-51 0.007524107892813295 0.0 0.0 1.0925390512051716 6.752404519191419E-4 52-53 0.007536165758026138 0.0 0.0 1.2820525187554064 7.114140475576673E-4 54-55 0.007608512949303188 0.0 0.0 1.4977074380870823 7.717033736218764E-4 56-57 0.008054653962178335 1.205786521284182E-5 0.0 1.759953948601179 7.958191040475601E-4 58-59 0.008078769692604019 2.411573042568364E-5 0.0 2.0501988221394942 7.958191040475601E-4 60-61 0.008102885423029702 2.411573042568364E-5 0.0 2.3597483378835697 7.958191040475601E-4 62-63 0.008235521940370963 2.411573042568364E-5 0.0 2.6711306491399966 8.199348344732437E-4 64-65 0.00828375340122233 4.823146085136728E-5 0.0 3.007822419478179 8.199348344732437E-4 66-67 0.008802241605374529 4.823146085136728E-5 0.0 3.3978823012483987 8.199348344732437E-4 68-69 0.008922820257502946 4.823146085136728E-5 0.0 3.8325442264409206 8.319926996860855E-4 70-71 0.009163977561759781 4.823146085136728E-5 0.0 4.318331500135892 8.68166295324611E-4 72-73 0.009405134866016619 4.823146085136728E-5 0.0 4.808399315981422 9.646292170273456E-4 74-75 0.009573944978996406 4.823146085136728E-5 0.0 5.30015523295675 9.646292170273456E-4 76-77 0.009598060709422089 4.823146085136728E-5 0.0 5.861243874905918 9.646292170273456E-4 78-79 0.00961011857463493 4.823146085136728E-5 0.0 6.4769425884040475 9.646292170273456E-4 80-81 0.010695326443790693 4.823146085136728E-5 0.0 7.131720843056996 9.766870822401873E-4 82-83 0.010827962961131954 4.823146085136728E-5 0.0 7.808275602284338 0.0010369764083043964 84-85 0.011045004534963106 4.823146085136728E-5 0.0 8.480887439587082 0.00106109213873008 86-87 0.011286161839219943 4.823146085136728E-5 0.0 9.19863186638149 0.0010852078691557638 88-89 0.011310277569645626 4.823146085136728E-5 0.0 9.98328538724196 0.0011093235995814474 90-91 0.01192522869550056 4.823146085136728E-5 0.0 10.814868119510807 0.001133439330007131 92-93 0.01205786521284182 4.823146085136728E-5 0.0 11.673351949069506 0.001133439330007131 94-95 0.01205786521284182 4.823146085136728E-5 0.0 12.527036748273495 0.0011816707908584984 96-97 0.01205786521284182 4.823146085136728E-5 0.0 13.40371589643837 0.0011816707908584984 98-99 0.012576353416994018 4.823146085136728E-5 0.0 14.330290490853988 0.0011816707908584984 100-101 0.013094841621146215 4.823146085136728E-5 0.0 15.336856963091599 0.0011816707908584984 102-103 0.013528924768808522 4.823146085136728E-5 0.0 16.361847853374428 0.0012419601169227075 104-105 0.01498792645956238 4.823146085136728E-5 0.0 17.373526860462285 0.0012781337125612328 106-107 0.015530530394140263 4.823146085136728E-5 0.0 18.38223963270778 0.0013022494429869164 108-109 0.015687282641907205 4.823146085136728E-5 0.0 19.43001988824288 0.0013384230386254419 110-111 0.015868150620099834 4.823146085136728E-5 0.0 20.543358756940208 0.0013987123646896512 112-113 0.015928439946164042 4.823146085136728E-5 0.0 21.68024663639821 0.0014228280951153348 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8455 0.0 32.7768 1 GTACATG 16255 0.0 30.174501 1 TACATGG 16395 0.0 28.921373 2 TCAACGC 9400 0.0 28.704561 4 CAACGCA 9470 0.0 28.492386 5 ACATGGG 15980 0.0 28.442324 3 ATCAACG 9700 0.0 27.941965 3 CATGGGG 10780 0.0 27.847895 4 AACGCAG 9710 0.0 27.83032 6 TATCAAC 10315 0.0 27.084505 2 GGTATCA 6370 0.0 24.88682 1 ACGCAGA 11030 0.0 24.01177 7 CGCAGAG 11360 0.0 23.10217 8 ATGGGGA 6510 0.0 21.501436 5 GCAGAGT 13365 0.0 19.05756 9 GTATAGA 965 0.0 18.527685 1 GTATAGG 815 0.0 18.281406 1 TAGACCG 410 1.081753E-8 17.433233 5 GTAATAC 825 0.0 17.329044 3 CTATACT 1140 0.0 17.24208 4 >>END_MODULE