FastQCFastQC Report
Mon 15 Aug 2016
SRR2920948_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920948_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4146671
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60070.14486319266708161No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48820.11773299593818753No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA109750.042.837981
GGTATCA72150.038.6178251
TCAACGC134450.034.0160454
ATCAACG135500.033.8842963
TATCAAC139100.033.1801952
CAACGCA137950.033.1530045
AACGCAG141200.032.5405466
GTACATG162400.029.2438351
TACATGG159900.028.6406082
CATGGGG107400.027.889914
ACGCAGA164500.027.7786967
ACATGGG161850.027.7054773
CGCAGAG166200.027.2061868
GAGTACT89850.023.31078112-13
GCAGAGT191450.023.2470599
AGTACTT95600.022.7489712-13
ATGGGGA64050.021.6630785
AGAGTAC169650.021.46494510-11
GTACTTT98300.021.21615814-15
CAGAGTA176850.020.01908110-11
Produced by FastQC (version 0.11.5)