##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2920948_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4146671 Sequences flagged as poor quality 0 Sequence length 125 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.145505394568318 31.0 31.0 34.0 28.0 34.0 2 31.27403090334391 33.0 31.0 34.0 28.0 34.0 3 31.38314783111561 33.0 31.0 34.0 28.0 34.0 4 34.939535111418294 37.0 35.0 37.0 32.0 37.0 5 34.6291632492667 37.0 35.0 37.0 32.0 37.0 6 34.38660409760022 37.0 35.0 37.0 30.0 37.0 7 34.29207766905067 37.0 35.0 37.0 30.0 37.0 8 34.29121504937334 37.0 35.0 37.0 30.0 37.0 9 35.85690183764277 39.0 37.0 39.0 30.0 39.0 10-11 35.82013885355265 39.0 36.0 39.0 30.0 39.0 12-13 35.703180454875735 39.0 35.0 39.0 30.0 39.0 14-15 36.94128446650337 40.0 37.0 41.0 30.0 41.0 16-17 36.939954604549044 40.0 37.0 41.0 30.0 41.0 18-19 36.96437129929045 40.0 37.0 41.0 30.0 41.0 20-21 36.95420398676433 40.0 37.0 41.0 30.0 41.0 22-23 36.901715255442255 40.0 37.0 41.0 30.0 41.0 24-25 36.87835121233394 40.0 37.0 41.0 30.0 41.0 26-27 36.76530088835116 40.0 37.0 41.0 30.0 41.0 28-29 36.67527674609343 40.0 36.5 41.0 30.0 41.0 30-31 36.54699480619514 40.0 36.0 41.0 30.0 41.0 32-33 36.41946901502434 40.0 36.0 41.0 29.5 41.0 34-35 36.31302049282424 40.0 36.0 41.0 29.0 41.0 36-37 36.19663375753707 40.0 36.0 41.0 29.0 41.0 38-39 36.08458881835574 40.0 35.0 41.0 28.0 41.0 40-41 35.95745804284931 40.0 35.0 41.0 27.5 41.0 42-43 35.81315771133037 40.0 35.0 41.0 27.0 41.0 44-45 35.63092188890799 40.0 35.0 41.0 26.5 41.0 46-47 35.44254463399676 39.5 35.0 41.0 26.0 41.0 48-49 35.20866183982284 39.0 35.0 41.0 24.5 41.0 50-51 35.03086270890553 39.0 35.0 41.0 24.0 41.0 52-53 34.83018148775247 39.0 34.0 41.0 24.0 41.0 54-55 34.62480023131809 39.0 34.0 41.0 23.0 41.0 56-57 34.372287673654355 38.0 34.0 41.0 23.0 41.0 58-59 34.13276505418443 38.0 34.0 40.0 22.5 41.0 60-61 33.85786791380363 37.5 33.5 40.0 22.0 41.0 62-63 33.55021172405527 37.0 33.0 40.0 21.5 41.0 64-65 33.22330913159014 36.5 33.0 39.5 20.5 41.0 66-67 32.89292567459536 36.0 33.0 39.0 20.0 41.0 68-69 32.520384785771526 35.5 32.0 39.0 19.0 41.0 70-71 32.186124725110815 35.0 32.0 38.5 18.0 40.0 72-73 31.81037162099429 35.0 32.0 37.0 17.5 39.5 74-75 31.4744558948612 35.0 31.5 37.0 16.0 39.0 76-77 31.262424243447335 35.0 31.5 36.0 16.0 39.0 78-79 31.116716638479396 35.0 32.0 36.0 15.0 38.0 80-81 30.876651053338932 35.0 31.5 35.5 12.5 37.0 82-83 30.66510774546618 35.0 31.0 35.0 11.5 37.0 84-85 30.437013329487677 35.0 31.0 35.0 9.0 36.0 86-87 30.279499868689847 35.0 31.0 35.0 7.0 36.0 88-89 30.141377263834052 35.0 31.0 35.0 7.0 36.0 90-91 29.99431556060271 35.0 31.0 35.0 7.0 35.5 92-93 29.883798955837104 35.0 31.0 35.0 3.5 35.0 94-95 29.800768978296084 35.0 31.0 35.0 2.0 35.0 96-97 29.73973568194824 35.0 31.0 35.0 2.0 35.0 98-99 29.642422198433394 34.0 31.0 35.0 2.0 35.0 100-101 29.54921502091678 34.0 31.0 35.0 2.0 35.0 102-103 28.118815671655646 32.5 28.0 34.5 2.0 35.0 104-105 29.210951628426756 34.0 30.0 35.0 2.0 35.0 106-107 29.393348423349718 34.0 30.0 35.0 2.0 35.0 108-109 29.496444979599296 34.5 30.5 35.0 2.0 35.0 110-111 29.684293255963638 35.0 31.0 35.0 2.0 35.0 112-113 29.66789769431913 35.0 31.0 35.0 2.0 35.0 114-115 29.59420520702028 35.0 31.0 35.0 2.0 35.0 116-117 29.482317502401322 35.0 31.0 35.0 2.0 35.0 118-119 29.387105222478468 35.0 31.0 35.0 2.0 35.0 120-121 29.207235394368155 35.0 31.0 35.0 2.0 35.0 122-123 29.01823402435351 35.0 31.0 35.0 2.0 35.0 124-125 28.28328193869251 34.5 29.0 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 72792.0 3 29206.0 4 12651.0 5 10446.0 6 11601.0 7 13487.0 8 15307.0 9 17271.0 10 19495.0 11 21284.0 12 22450.0 13 25656.0 14 29676.0 15 27390.0 16 17907.0 17 16399.0 18 15024.0 19 14477.0 20 14278.0 21 15041.0 22 16504.0 23 19206.0 24 26068.0 25 35022.0 26 43084.0 27 52068.0 28 62783.0 29 75412.0 30 90250.0 31 110518.0 32 137720.0 33 176396.0 34 239515.0 35 355149.0 36 672728.0 37 1190989.0 38 420548.0 39 873.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.16011967047122 17.823070398232975 11.738943410270105 26.277866521025707 2 16.768302756486523 20.2701474002666 36.71536918560973 26.246180657637154 3 19.364411929828623 23.504382334840095 29.108836824345836 28.022368910985453 4 13.543766551382266 15.751429438929696 35.00029060254086 35.70451340714718 5 14.220964362509783 36.95290081828194 34.17147113349535 14.654663685712926 6 34.47735455207248 35.17096738311359 16.268509673445074 14.083168391368856 7 29.440962415069 31.2702158520503 20.58727253736578 18.701549195514925 8 27.2273363810858 34.521078840696326 19.412682953184344 18.83890182503354 9 26.980495330051202 14.42783117756418 18.67875807629561 39.912915416089014 10-11 26.04850567673888 25.146252286294267 27.328528984971566 21.47671305199528 12-13 26.453858782992253 22.531063626981297 27.408952103513506 23.606125486512948 14-15 23.60552341701789 23.852136408051873 24.82897615809089 27.713364016839343 16-17 23.959991060559883 26.762129241485127 25.45134101853414 23.826538679420846 18-19 23.79671017925333 25.81000251586163 27.08868979088438 23.304597514000662 20-21 24.50047932325273 25.24393989844787 26.868211974120765 23.38736880417863 22-23 24.24736723095708 25.108762832205255 26.649405309254266 23.9944646275834 24-25 23.88279345081387 25.2762483407734 26.941028309869907 23.899929898542823 26-27 23.840379009889002 25.359804822352377 26.912791716406325 23.88702445135229 28-29 23.800030583223027 25.523990946594168 26.9955914814629 23.680386988719903 30-31 23.97578273196156 25.34872166542256 26.806111115800796 23.869384486815086 32-33 23.817209851125554 25.36739841641816 26.93030995329524 23.88508177916105 34-35 24.007778699027664 25.464180296977464 26.806602849174645 23.72143815482023 36-37 24.01984910559991 25.281499377876276 26.764815523461305 23.93383599306251 38-39 23.904122566577833 25.450581025467045 26.840625554410735 23.804670853544387 40-41 24.094637172231568 25.322335073133857 26.760335925103828 23.822691829530747 42-43 24.020284233503382 25.27238833016164 26.671593763443635 24.035733672891347 44-45 23.95592165699373 25.463044872270324 26.60042444900564 23.98060902173031 46-47 24.0515079679795 25.479866102053045 26.387099948767972 24.081525981199487 48-49 23.992544931992523 25.61751000294387 26.24810822342869 24.14183684163492 50-51 23.889164251714586 25.732824896980134 26.24682076533178 24.1311900859735 52-53 24.029539307256286 25.693413615301857 26.079544173232115 24.19750290420974 54-55 23.830939665879907 25.48605497304629 26.188567536951226 24.494437824122578 56-57 23.738873011952407 25.7821334293025 26.20933933719526 24.26965422154984 58-59 23.78953240988584 25.581100300425753 26.287114688685907 24.342252601002496 60-61 23.934096805748702 25.52516215959691 26.32999331739526 24.210747717259125 62-63 23.767831862174802 25.617516310670663 26.312204822511774 24.302447004642755 64-65 23.560893694934908 25.425825438162963 26.38213420571559 24.63114666118654 66-67 23.484202700968602 25.82587754464066 26.242482702859682 24.447437051531054 68-69 23.52054072254564 25.457379725268257 26.4399513942086 24.582128157977507 70-71 23.29975189282272 25.308837538339752 26.38203102491431 25.00937954392322 72-73 23.496762524938678 25.438234456248193 26.301641544464836 24.76336147434829 74-75 23.321666418036713 25.425148174004946 26.21598437743643 25.03720103052191 76-77 23.33674496537055 25.34450669959392 26.364559353774908 24.954188981260625 78-79 23.328166105121536 25.424077321737258 26.308447585999133 24.939308987142066 80-81 23.131828261184957 25.390208319740783 26.336648087529095 25.141315331545165 82-83 23.099219045549425 25.37902381356945 26.279679832673462 25.242077308207662 84-85 23.080034539648235 25.29757884784224 26.143124873370706 25.479261739138824 86-87 23.04499929087115 25.22715037294389 26.35813853193926 25.369711804245703 88-89 22.925109496744916 25.142746465513692 26.377761930794513 25.55438210694688 90-91 22.835902035803535 25.218740284607783 26.34283754453252 25.60252013505616 92-93 22.815449155247556 25.17524969818426 26.29139211011261 25.717909036455577 94-95 22.64935109414414 25.072330116932935 26.436614023148774 25.841704765774153 96-97 22.718385882331376 24.915021874947993 26.36363570583881 26.002956536881822 98-99 22.610340249561126 24.856814873959827 26.313261427525532 26.21958344895352 100-101 22.430622028034477 24.921172701761403 26.41929236392168 26.228912906282435 102-103 22.323772969675524 24.776681825708767 26.4763301730789 26.423215031536813 104-105 22.294151816114386 24.793188013954964 26.429800654239877 26.48285951569077 106-107 22.14485967840876 24.888790757076613 26.41516919517537 26.551180369339257 108-109 22.03965751490154 24.867108420571007 26.40800916728191 26.685224897245547 110-111 21.864086371301667 25.00956299177655 26.41465952372683 26.711691113194952 112-113 21.86464056084986 25.037702479741476 26.30082297886852 26.796833980540143 114-115 21.76071645713164 25.083668905313832 26.287881327502415 26.86773331005211 116-117 21.595072657156543 25.031493469966016 26.419171664161134 26.9542622087163 118-119 21.612156280217032 25.026259690401538 26.252371089368076 27.109212940013354 120-121 21.596003534010755 25.113156955150934 26.238221119729626 27.052618391108684 122-123 21.39391998410264 25.120815819993293 26.22778270684833 27.257481489055735 124-125 21.3302260849937 25.12566473864375 26.165094533573317 27.37901464278923 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 66.0 1 79.0 2 210.5 3 368.0 4 284.0 5 194.5 6 350.5 7 720.5 8 1124.5 9 1245.0 10 1059.5 11 940.0 12 1016.5 13 1106.0 14 1318.5 15 1530.0 16 1706.0 17 1919.0 18 2131.5 19 2436.5 20 2831.0 21 3288.5 22 3858.0 23 4792.5 24 6061.5 25 7676.0 26 10335.5 27 13621.5 28 16471.5 29 20323.0 30 25047.0 31 30096.5 32 35815.5 33 41561.0 34 48074.5 35 54810.0 36 62753.0 37 71589.0 38 78982.0 39 85744.5 40 95180.5 41 104947.5 42 113009.5 43 123664.0 44 134725.0 45 144363.0 46 155123.5 47 165763.5 48 174953.5 49 181181.0 50 184534.5 51 185826.0 52 184218.0 53 183305.5 54 182576.5 55 176997.5 56 165109.5 57 150739.5 58 137205.0 59 120485.0 60 100486.5 61 80671.5 62 63403.0 63 49525.5 64 37742.5 65 28027.0 66 20756.0 67 16187.5 68 12169.0 69 8523.0 70 6238.5 71 4613.0 72 3465.5 73 2552.0 74 1751.5 75 1179.0 76 766.5 77 427.5 78 220.0 79 134.5 80 95.5 81 74.5 82 60.0 83 43.0 84 27.0 85 19.5 86 18.0 87 13.0 88 10.0 89 8.0 90 7.0 91 6.0 92 3.5 93 3.0 94 5.0 95 9.0 96 8.0 97 5.0 98 2.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20838402660833233 2 0.008247579805583804 3 5.546617997907237E-4 4 0.0027491932685279347 5 0.0838745104205277 6 0.05508032829226143 7 0.026527303468252005 8 0.01603696073307962 9 0.014155933759876296 10-11 0.011973460156351926 12-13 0.014083586568599244 14-15 0.02180062030481801 16-17 0.02405544109961943 18-19 0.023488721434615865 20-21 0.017399499502130746 22-23 0.014674421964028494 24-25 0.012962205103804955 26-27 0.012045807347628978 28-29 0.014638248368389967 30-31 0.010996773074111739 32-33 0.015120562976903642 34-35 0.01768888826723895 36-37 0.0190876006319286 38-39 0.02268084446535546 40-41 0.023753994469298385 42-43 0.021450942213645595 44-45 0.019907535466401845 46-47 0.01994370906204037 48-49 0.01848470737128651 50-51 0.015096447246477959 52-53 0.012889857912527905 54-55 0.01848470737128651 56-57 0.021583578730986857 58-59 0.017399499502130746 60-61 0.02007634557938163 62-63 0.025852063016332864 64-65 0.018858501192884604 66-67 0.02168004165268959 68-69 0.015988729272228254 70-71 0.016410754554677717 72-73 0.024163961886535006 74-75 0.014867347807433963 76-77 0.013540982634021363 78-79 0.016157539385208038 80-81 0.01718245792829959 82-83 0.012696932069122436 84-85 0.017146284332661067 86-87 0.017821524784580208 88-89 0.01860528602341493 90-91 0.0131189573515719 92-93 0.012431659034439914 94-95 0.013902718590406619 96-97 0.014565901177112917 98-99 0.01332394106019021 100-101 0.017857698380218733 102-103 0.023163159073869136 104-105 0.00801848036653981 106-107 0.007729091601431606 108-109 0.014999984324775222 110-111 0.011551434873902464 112-113 0.009453366326867985 114-115 0.006463015754083216 116-117 0.004256426420133162 118-119 0.0025683252903353077 120-121 0.0025924410207609915 122-123 0.0016881011297978548 124-125 5.426039345778819E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 4146671.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.50303489094055 #Duplication Level Percentage of deduplicated Percentage of total 1 68.813045554225 30.623893672515567 2 14.239030664925856 12.673601569887353 3 6.25546487986844 8.351615154235152 4 3.197409437169053 5.691776949718276 5 1.9153501255671528 4.261944673324118 6 1.2377424692405845 3.3049977776767583 7 0.8630603943391133 2.6886164789583726 8 0.5738386850039843 2.0430050416403005 9 0.43417581650695614 1.7389927359730502 >10 2.2509508835369147 17.836009629990336 >50 0.14929465914911394 4.548584502757101 >100 0.06852220199369762 5.196355191362402 >500 0.00168054061926607 0.4938738118986621 >1k 3.794768730084458E-4 0.40181073831038083 >5k 5.421098185834928E-5 0.14492207175205377 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6007 0.14486319266708161 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4882 0.11773299593818753 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 2.411573042568364E-5 0.0 0.0 0.0 0.0 3 2.411573042568364E-5 0.0 0.0 0.0 0.0 4 2.411573042568364E-5 0.0 0.0 0.0 0.0 5 9.646292170273456E-5 0.0 0.0 0.0 0.0 6 1.6881011297978547E-4 0.0 0.0 0.0 0.0 7 6.752404519191419E-4 0.0 0.0 0.0 0.0 8 6.993561823448255E-4 0.0 0.0 0.0 2.411573042568364E-5 9 6.993561823448255E-4 0.0 0.0 0.0 4.823146085136728E-5 10-11 7.717033736218765E-4 0.0 0.0 0.0 4.823146085136728E-5 12-13 9.40513486601662E-4 0.0 0.0 0.0 4.823146085136728E-5 14-15 0.001133439330007131 0.0 0.0 0.0 4.823146085136728E-5 16-17 0.0011696129256456565 0.0 0.0 6.02893260642091E-5 4.823146085136728E-5 18-19 0.0016278118037336455 0.0 0.0 8.440505648989274E-5 4.823146085136728E-5 20-21 0.0020498370861831094 0.0 0.0 1.0852078691557637E-4 4.823146085136728E-5 22-23 0.0022548207948014203 0.0 0.0 1.5675224776694365E-4 1.3263651734126002E-4 24-25 0.003062697764061822 0.0 0.0 3.014466303210455E-4 2.1704157383115274E-4 26-27 0.0031229870901260314 0.0 0.0 6.270089910677746E-4 2.1704157383115274E-4 28-29 0.0036776488899167552 0.0 0.0 0.002604498885973833 2.1704157383115274E-4 30-31 0.004123789902791902 0.0 0.0 0.009525713518145037 2.1704157383115274E-4 32-33 0.004208194959281795 0.0 0.0 0.027262833246235352 2.411573042568364E-4 34-35 0.0045096415896028405 0.0 0.0 0.05563499009205215 2.6527303468252005E-4 36-37 0.004871377545988095 0.0 0.0 0.10745969477684629 2.6527303468252005E-4 38-39 0.005257229232799033 0.0 0.0 0.1976766422993288 2.6527303468252005E-4 40-41 0.005281344963224717 0.0 0.0 0.30737910000576363 2.6527303468252005E-4 42-43 0.0055707337283329205 0.0 0.0 0.4151281835477182 2.6527303468252005E-4 44-45 0.006053048336846593 0.0 0.0 0.5521537638264526 2.7733089989536184E-4 46-47 0.0069935618234482555 0.0 0.0 0.720734777367194 3.014466303210455E-4 48-49 0.00748793429717477 0.0 0.0 0.8934878122715788 3.135044955338873E-4 50-51 0.007524107892813295 0.0 0.0 1.0856660680338517 3.135044955338873E-4 52-53 0.007536165758026138 0.0 0.0 1.2738772861410999 3.858516868109382E-4 54-55 0.007596455084090346 0.0 0.0 1.4892669324380932 4.461410128751473E-4 56-57 0.008247579805583804 0.0 0.0 1.7515013850869772 4.5819887808798914E-4 58-59 0.008247579805583804 0.0 0.0 2.0421079945816776 4.5819887808798914E-4 60-61 0.008295811266435173 0.0 0.0 2.3511872535824523 4.943724737265146E-4 62-63 0.008368158457712223 0.0 0.0 2.6625213333780278 5.064303389393564E-4 64-65 0.008440505648989273 0.0 0.0 2.998754904838122 5.064303389393564E-4 66-67 0.008898704527077262 0.0 0.0 3.389586489981964 5.184882041521983E-4 68-69 0.008971051718354314 0.0 0.0 3.823380248879161 5.305460693650401E-4 70-71 0.00927249834867536 0.0 0.0 4.3060686512144315 5.787775302164073E-4 72-73 0.009453366326867985 0.0 0.0 4.7923382395179175 7.475876431961928E-4 74-75 0.009586002844209247 0.0 0.0 5.280886764346629 7.475876431961928E-4 76-77 0.009622176439847772 0.0 0.0 5.838924766396948 7.596455084090347E-4 78-79 0.00967040790069914 0.0 0.0 6.451331682691972 7.717033736218765E-4 80-81 0.010574747791662276 0.0 0.0 7.101841453059574 8.319926996860855E-4 82-83 0.010852078691557637 0.0 0.0 7.774561811149233 9.405134866016619E-4 84-85 0.011057062400175947 0.0 0.0 8.445521720917816 9.405134866016619E-4 86-87 0.01133439330007131 0.0 0.0 9.16142129433466 9.405134866016619E-4 88-89 0.011442914086986887 0.0 0.0 9.944157614626286 9.405134866016619E-4 90-91 0.012033749482416135 2.411573042568364E-5 0.0 10.772074755870431 9.405134866016619E-4 92-93 0.012081980943267503 2.411573042568364E-5 0.0 11.628677558455927 9.405134866016619E-4 94-95 0.012081980943267503 2.411573042568364E-5 0.0 12.481301265521186 9.646292170273456E-4 96-97 0.012081980943267503 2.411573042568364E-5 0.0 13.356678164243075 9.646292170273456E-4 98-99 0.012745163529973803 2.411573042568364E-5 0.0 14.28057591258144 9.646292170273456E-4 100-101 0.013335998925403051 2.411573042568364E-5 0.0 15.286623896614898 9.646292170273456E-4 102-103 0.0139268343208323 2.411573042568364E-5 0.0 16.30945642902463 9.766870822401873E-4 104-105 0.015409951742011846 3.617359563852546E-5 0.0 17.320206980491097 9.887449474530292E-4 106-107 0.015723456237545733 4.823146085136728E-5 0.0 18.331958334770228 0.001000802812665871 108-109 0.01584403488967415 4.823146085136728E-5 0.0 19.387636492019745 0.0010128606778787128 110-111 0.016049018598292462 4.823146085136728E-5 0.0 20.507631302314557 0.0010369764083043964 112-113 0.016133423654782354 4.823146085136728E-5 0.0 21.653176728995376 0.0010369764083043964 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 10975 0.0 42.83798 1 GGTATCA 7215 0.0 38.617825 1 TCAACGC 13445 0.0 34.016045 4 ATCAACG 13550 0.0 33.884296 3 TATCAAC 13910 0.0 33.180195 2 CAACGCA 13795 0.0 33.153004 5 AACGCAG 14120 0.0 32.540546 6 GTACATG 16240 0.0 29.243835 1 TACATGG 15990 0.0 28.640608 2 CATGGGG 10740 0.0 27.88991 4 ACGCAGA 16450 0.0 27.778696 7 ACATGGG 16185 0.0 27.705477 3 CGCAGAG 16620 0.0 27.206186 8 GAGTACT 8985 0.0 23.310781 12-13 GCAGAGT 19145 0.0 23.247059 9 AGTACTT 9560 0.0 22.74897 12-13 ATGGGGA 6405 0.0 21.663078 5 AGAGTAC 16965 0.0 21.464945 10-11 GTACTTT 9830 0.0 21.216158 14-15 CAGAGTA 17685 0.0 20.019081 10-11 >>END_MODULE