FastQCFastQC Report
Mon 15 Aug 2016
SRR2920947_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920947_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3589533
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG100250.031.0712154
GTACATG155200.030.6399881
GGTATCA63450.030.5231321
GTATCAA86800.029.5893171
TACATGG158950.029.228422
ACATGGG156400.028.8290523
TCAACGC96850.025.4590174
ATGGGGA52350.024.915435
TATCAAC102250.024.6404322
AACGCAG100250.024.6401926
CAACGCA99600.024.636495
ATCAACG102150.024.1397763
ACGCAGA113300.021.5898657
CGCAGAG117650.020.637828
ATGGGGG53600.019.3341665
GCAGAGT133150.017.4306269
AGAGTAC113850.016.75175510-11
GAGTACA116100.016.321961
CAGAGTA126100.016.1861810-11
ATGGGGC41100.015.9401275
Produced by FastQC (version 0.11.5)