##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2920947_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3589533 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.849625564105413 31.0 30.0 34.0 25.0 34.0 2 29.90178081661319 31.0 30.0 34.0 25.0 34.0 3 30.043282231978367 31.0 31.0 34.0 25.0 34.0 4 33.46623892300196 37.0 35.0 37.0 27.0 37.0 5 33.534037436067585 37.0 35.0 37.0 28.0 37.0 6 33.51038031966832 37.0 35.0 37.0 28.0 37.0 7 33.447382709672816 37.0 35.0 37.0 27.0 37.0 8 33.371375329325566 37.0 35.0 37.0 27.0 37.0 9 34.79198798283788 39.0 35.0 39.0 27.0 39.0 10-11 34.793788774194304 39.0 35.0 39.0 27.0 39.0 12-13 34.73814393125791 39.0 35.0 39.0 27.0 39.0 14-15 35.90026502054724 40.0 36.0 41.0 27.0 41.0 16-17 35.88558497721013 40.0 36.0 41.0 27.0 41.0 18-19 35.86358336864433 40.0 36.0 41.0 27.0 41.0 20-21 35.897205430344286 40.0 36.0 41.0 27.0 41.0 22-23 35.824812308453495 40.0 36.0 41.0 27.0 41.0 24-25 35.82990322139398 40.0 36.0 41.0 27.0 41.0 26-27 35.76799363594094 40.0 36.0 41.0 27.0 41.0 28-29 35.67234317110332 40.0 36.0 41.0 26.5 41.0 30-31 35.5317693137241 40.0 35.0 41.0 25.5 41.0 32-33 35.41504591265772 40.0 35.0 41.0 25.0 41.0 34-35 35.355467131796814 40.0 35.0 41.0 25.0 41.0 36-37 35.22277243307138 40.0 35.0 41.0 25.0 41.0 38-39 35.08438771840237 39.5 35.0 41.0 24.0 41.0 40-41 34.95900399857029 39.0 35.0 41.0 24.0 41.0 42-43 34.768706124167124 39.0 34.0 41.0 23.5 41.0 44-45 34.60444450573375 39.0 34.0 41.0 23.0 41.0 46-47 34.40410061698834 39.0 34.0 41.0 22.0 41.0 48-49 34.2843038356243 39.0 33.5 41.0 21.5 41.0 50-51 34.08561824059007 38.5 33.0 40.0 20.0 41.0 52-53 33.886705875109655 38.0 33.0 40.0 19.5 41.0 54-55 33.639366457976564 38.0 33.0 40.0 18.0 41.0 56-57 33.36994659193828 38.0 32.5 40.0 17.0 41.0 58-59 33.10054859504008 37.0 32.0 40.0 14.0 41.0 60-61 32.82207532289019 37.0 32.0 40.0 13.0 41.0 62-63 32.50099093670402 36.0 31.5 40.0 9.5 41.0 64-65 32.20124470230529 36.0 31.0 39.0 8.0 41.0 66-67 31.845055888885824 35.5 31.0 39.0 7.5 40.5 68-69 31.506396235944898 35.0 31.0 38.5 7.0 40.0 70-71 31.1595331481839 35.0 30.5 38.0 6.5 40.0 72-73 31.21734721480482 35.0 31.0 37.0 2.0 39.5 74-75 31.208728823498767 35.0 31.0 37.0 2.0 39.0 76-77 30.9544106433901 35.0 31.0 36.5 2.0 39.0 78-79 30.67404004922089 35.0 31.0 36.0 2.0 39.0 80-81 30.416022362797612 35.0 31.0 36.0 2.0 37.0 82-83 30.189069859505402 35.0 31.0 35.0 2.0 37.0 84-85 29.96841831514016 35.0 31.0 35.0 2.0 36.5 86-87 29.78204713537945 35.0 31.0 35.0 2.0 36.0 88-89 29.600440642278535 35.0 30.5 35.0 2.0 36.0 90-91 29.447500552300255 35.0 30.0 35.0 2.0 36.0 92-93 29.297538844189482 35.0 30.0 35.0 2.0 35.0 94-95 29.193223046006263 34.0 30.0 35.0 2.0 35.0 96-97 29.107436399108185 34.0 30.0 35.0 2.0 35.0 98-99 28.986580148448283 34.0 30.0 35.0 2.0 35.0 100-101 28.876231532068378 34.0 30.0 35.0 2.0 35.0 102-103 27.507774409651617 32.5 26.5 34.5 2.0 35.0 104-105 28.541441184688928 34.0 29.0 35.0 2.0 35.0 106-107 28.69981666138743 34.0 29.5 35.0 2.0 35.0 108-109 28.640217543619183 34.0 29.5 35.0 2.0 35.0 110-111 28.545836603257307 34.0 29.0 35.0 2.0 35.0 112-113 28.437906825205395 34.0 29.0 35.0 2.0 35.0 114-115 28.331855843085997 34.0 29.0 35.0 2.0 35.0 116-117 28.132470435569196 34.0 29.0 35.0 2.0 35.0 118-119 27.947924702182707 34.0 29.0 35.0 2.0 35.0 120-121 27.78607077856646 34.0 29.0 35.0 2.0 35.0 122-123 27.512211755679637 34.0 29.0 35.0 2.0 35.0 124-125 26.25897505330081 33.0 24.0 34.5 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 129987.0 3 19571.0 4 12830.0 5 12048.0 6 14535.0 7 15466.0 8 15570.0 9 16961.0 10 18669.0 11 19935.0 12 20890.0 13 20313.0 14 19912.0 15 17793.0 16 14483.0 17 13685.0 18 13108.0 19 13055.0 20 13568.0 21 14658.0 22 17691.0 23 22701.0 24 31790.0 25 41189.0 26 45577.0 27 50008.0 28 58666.0 29 68969.0 30 81506.0 31 99430.0 32 124111.0 33 160973.0 34 216099.0 35 320153.0 36 580221.0 37 920441.0 38 312527.0 39 444.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.17038594344988 18.417577719722892 12.024170229675224 25.387866107152007 2 16.08647168335825 20.539228828953497 38.24775600312582 25.126543484562436 3 19.03711955760377 24.563360880773928 29.152418070689407 27.247101490932895 4 12.990214814314704 16.107167786612983 35.99457984297503 34.90803755609729 5 13.757309925201286 37.969305848721525 34.147187485698275 14.126196740378916 6 32.97318176493884 35.982507906582406 16.98166565335981 14.062644675118946 7 28.396893086696778 32.37775474170736 21.153359710470617 18.071992461125237 8 26.364938361940116 35.59974412285673 19.650673043889512 18.384644471313653 9 26.434515034945726 15.938330870873987 19.03952372745511 38.58763036672517 10-11 25.253029551018585 26.249616600768473 27.51780366613316 20.97955018207978 12-13 25.371203090709287 23.8355787043394 27.484439601929267 23.30877860302204 14-15 23.01870485144015 25.18094274235594 24.59266972791676 27.20768267828715 16-17 23.217810574868516 27.921906295584215 25.497063094128197 23.363220035419076 18-19 23.336126853704286 27.171794811746835 26.753575260003704 22.738503074545175 20-21 24.14647942451306 26.40675545930901 26.37949097372685 23.067274142451083 22-23 23.89203361938588 26.169996079327213 26.19806659122659 23.73990371006032 24-25 23.544559343486966 26.374327114419366 26.367148924853556 23.713964617240116 26-27 23.404611820333606 26.54054928112811 26.394920919910675 23.659917978627607 28-29 23.495070793098762 26.633203583737274 26.44793928137077 23.423786341793193 30-31 23.663224535793212 26.52813117870855 26.2479658393426 23.560678446155634 32-33 23.328261990213615 26.672198027166676 26.24095264302289 23.75858733959682 34-35 23.58872887221116 26.652262610112626 26.369641081112725 23.38936743656349 36-37 23.74492850596103 26.400373588153908 26.252276552812887 23.60242135307217 38-39 23.567259955929845 26.636809842632076 26.217686160722252 23.57824404071583 40-41 23.56024598893507 26.554102956707155 26.41268273806271 23.472968316295066 42-43 23.498147876836633 26.532136528931748 26.271627909829558 23.69808768440206 44-45 23.463978776173125 26.59859962105885 26.31060936263146 23.626812240136566 46-47 23.516430917776624 26.57920812848959 26.29780366808463 23.606557285649153 48-49 23.393521458273984 26.727022969365073 26.182977214608382 23.696478357752557 50-51 23.284581148052354 26.84749744791147 26.178267635057196 23.689653768978978 52-53 23.506945349479516 26.693455549888778 26.104665093194956 23.694934007436753 54-55 23.368173472727342 26.69053615325387 26.11637496773201 23.82491540628678 56-57 23.349646356026458 26.790003321706664 26.106105319149382 23.754245003117497 58-59 23.358387655483813 26.534566197947495 26.21335110258019 23.8936950439885 60-61 23.332374342994047 26.65049485574165 26.309077568379635 23.70805323288467 62-63 23.248067222346194 26.563857451628465 26.35509084302376 23.832984483001578 64-65 23.236708141840214 26.552008892017042 26.340774706532077 23.87050825961066 66-67 23.34458223873474 26.705747924908678 26.10765386450914 23.842015971847445 68-69 23.434482537527607 26.474380730891916 26.213591827034648 23.877544904545825 70-71 23.23665043097116 26.467352202718263 26.33795353299903 23.958043833311542 72-73 23.330027450534406 26.477556790941097 26.228291555448518 23.964124203075976 74-75 23.203741954546846 26.65660375254709 26.233794108874598 23.905860184031464 76-77 23.31995182657567 26.415651902404207 26.371367478478074 23.89302879254204 78-79 23.264235163755345 26.577504590864265 26.287382876337624 23.870877369042766 80-81 23.269121678785623 26.56770796310931 26.302707705259166 23.8604626528459 82-83 23.27202512505659 26.51716837980629 26.368890179907538 23.841916315229582 84-85 23.267033396685214 26.63297656956197 26.128224481702876 23.971765552049945 86-87 23.209950843153713 26.43560667777129 26.407926421250856 23.946516057824137 88-89 23.230187816689348 26.408123415028818 26.29281730609082 24.068871462191012 90-91 23.190363584514188 26.4757092293973 26.35090815984907 23.983019026239447 92-93 23.322277388897934 26.48885913134228 26.334490309718824 23.854373170040965 94-95 23.28858793790888 26.384780664859754 26.38880893523336 23.937822461998007 96-97 23.356075846781753 26.218566448161074 26.406949996899066 24.018407708158108 98-99 23.265255608875975 26.367852734367542 26.255857089320152 24.11103456743633 100-101 23.167309500808024 26.457010199684593 26.372193999706784 24.0034862998006 102-103 23.140241331903486 26.395135176943512 26.40760801020682 24.057015480946177 104-105 23.09922368830694 26.574858070110256 26.26049266652601 24.065425575056796 106-107 23.038590824313253 26.591105292074417 26.259756557496544 24.110547326115782 108-109 23.006158141474465 26.725949324394012 26.27169858445256 23.996193949678965 110-111 22.989042137647345 26.85863833478851 26.163220520986307 23.989099006577842 112-113 23.062698301301033 26.94307920706102 26.13585884090498 23.858363650732965 114-115 23.028249623929355 27.12878565701688 26.043268474853726 23.799696244200035 116-117 22.968238663680104 27.04862103809205 26.08295530847818 23.900184989749665 118-119 23.095880287525592 27.01605152835957 25.951218329136267 23.936849854978572 120-121 23.04554201625738 27.24217774954135 25.928956272226777 23.783323961974492 122-123 23.077095695917983 27.304322591921597 25.824701186334227 23.793880525826193 124-125 23.1989327690503 27.388901550872248 25.671936607373734 23.740229072703713 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2339.0 1 1760.5 2 954.5 3 658.5 4 547.0 5 478.5 6 458.5 7 490.5 8 558.0 9 601.5 10 610.5 11 660.0 12 718.5 13 806.0 14 908.5 15 1008.0 16 1148.0 17 1331.0 18 1550.5 19 1819.0 20 2274.5 21 2930.0 22 3836.5 23 5030.0 24 6425.5 25 8218.5 26 11346.5 27 15307.5 28 18687.5 29 22750.5 30 27997.0 31 33955.0 32 40890.5 33 46944.0 34 52336.0 35 58456.5 36 66223.0 37 74531.5 38 81523.5 39 87656.0 40 93781.0 41 100325.0 42 107636.0 43 116895.0 44 123963.5 45 130925.0 46 139268.0 47 144172.0 48 147888.0 49 150622.5 50 153737.0 51 154871.0 52 150072.0 53 145454.5 54 140671.0 55 133220.5 56 123753.5 57 112121.0 58 101796.5 59 91202.0 60 77193.5 61 63548.5 62 51509.5 63 38899.5 64 28093.0 65 20776.5 66 15639.5 67 11874.5 68 8962.5 69 6659.5 70 5002.5 71 3649.0 72 2585.5 73 2000.0 74 1556.0 75 1122.0 76 767.5 77 488.5 78 314.0 79 221.0 80 145.0 81 106.0 82 88.0 83 73.0 84 60.5 85 45.5 86 32.5 87 26.5 88 21.0 89 15.5 90 10.5 91 7.5 92 7.5 93 7.5 94 9.0 95 12.0 96 10.5 97 12.0 98 12.0 99 13.0 100 16.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16578758295299137 2 0.1446427710791348 3 0.17102503306140382 4 0.14305482078030765 5 0.16851774311588721 6 0.10901139507562684 7 0.10731200966811003 8 0.09575061714156131 9 0.10271531143466295 10-11 0.0889530755114941 12-13 0.07754490625939363 14-15 0.0769738013273593 16-17 0.07702951888170412 18-19 0.07413220605577382 20-21 0.06822614529522364 22-23 0.0595759949831914 24-25 0.06344836501015592 26-27 0.07418792361011864 28-29 0.06952157843374054 30-31 0.06589993740132769 32-33 0.07555300369156656 34-35 0.0718895744933951 36-37 0.07509333386822185 38-39 0.07034341236032654 40-41 0.07535799225135972 42-43 0.07531620408560111 44-45 0.06776647547187893 46-47 0.06539847941222438 48-49 0.05736122219798509 50-51 0.06337871806722489 52-53 0.06542633818939678 54-55 0.06649890111053443 56-57 0.07024590664022312 58-59 0.0574308691409161 60-61 0.0676132521974307 62-63 0.06333692990146629 64-65 0.0569851287061576 66-67 0.06187434409991495 68-69 0.06520346797201754 70-71 0.06045354646412221 72-73 0.055411107795916625 74-75 0.05858700839357098 76-77 0.06945193149080953 78-79 0.06255688414063891 80-81 0.06067641668150147 82-83 0.06566313779536223 84-85 0.0675714640316721 86-87 0.0595759949831914 88-89 0.05921383087995012 90-91 0.06195792043143216 92-93 0.05630258866543364 94-95 0.06645711294477583 96-97 0.053084899902020685 98-99 0.052750594575951805 100-101 0.04944932948102162 102-103 0.048348907782711566 104-105 0.046524157877918935 106-107 0.05777910385557118 108-109 0.05814126795881247 110-111 0.06510596225191412 112-113 0.06537062063505197 114-115 0.06800327507784439 116-117 0.07180599816187788 118-119 0.06420055199381089 120-121 0.06580243168122427 122-123 0.06307227151832842 124-125 0.07467545221063576 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3589533.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.56824169386161 #Duplication Level Percentage of deduplicated Percentage of total 1 69.75215009747743 33.179871345033185 2 14.050614708504527 13.367260728029404 3 5.951694552781265 8.493349349242164 4 3.1641263713312955 6.020477119256334 5 1.8627481381496331 4.430382682514623 6 1.19073054689468 3.3984575068170098 7 0.7774289346117864 2.588664922299035 8 0.5955425219052674 2.2663128496769303 9 0.41523009350746787 1.7776588901875185 >10 2.092082879071451 17.460310944089137 >50 0.10912810191909372 3.5091692063040107 >100 0.037111089102898995 2.871699116815665 >500 0.0010001417000576978 0.31349114521218685 >1k 4.1182304323373773E-4 0.3228941945228872 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 2.785877717240655E-5 0.0 0.0 2.785877717240655E-5 12-13 0.0 2.785877717240655E-5 0.0 0.0 2.785877717240655E-5 14-15 0.0 2.785877717240655E-5 0.0 0.0 2.785877717240655E-5 16-17 0.0 2.785877717240655E-5 0.0 0.0 2.785877717240655E-5 18-19 0.0 2.785877717240655E-5 0.0 4.178816575860982E-5 2.785877717240655E-5 20-21 0.0 2.785877717240655E-5 0.0 9.750572010342292E-5 5.57175543448131E-5 22-23 0.0 2.785877717240655E-5 0.0 2.3679960596545565E-4 5.57175543448131E-5 24-25 0.0 2.785877717240655E-5 0.0 3.0644654889647204E-4 5.57175543448131E-5 26-27 0.0 2.785877717240655E-5 0.0 7.103988178963671E-4 5.57175543448131E-5 28-29 0.0 2.785877717240655E-5 0.0 0.0026047956656200123 5.57175543448131E-5 30-31 0.0 2.785877717240655E-5 0.0 0.010795276154307538 5.57175543448131E-5 32-33 0.0 2.785877717240655E-5 0.0 0.030937172049957475 5.57175543448131E-5 34-35 0.0 2.785877717240655E-5 0.0 0.06413090505087987 5.57175543448131E-5 36-37 0.0 2.785877717240655E-5 0.0 0.12112996314562367 5.57175543448131E-5 38-39 0.0 2.785877717240655E-5 0.0 0.2173681088877021 5.57175543448131E-5 40-41 0.0 2.785877717240655E-5 0.0 0.3275077844388114 5.57175543448131E-5 42-43 0.0 2.785877717240655E-5 0.0 0.4351123112672317 5.57175543448131E-5 44-45 0.0 2.785877717240655E-5 0.0 0.5662017872519907 5.57175543448131E-5 46-47 0.0 2.785877717240655E-5 0.0 0.7255400632895699 5.57175543448131E-5 48-49 0.0 2.785877717240655E-5 0.0 0.8970804837286633 5.57175543448131E-5 50-51 0.0 2.785877717240655E-5 0.0 1.0813662947241327 5.57175543448131E-5 52-53 1.3929388586203274E-5 2.785877717240655E-5 0.0 1.262378699401844 5.57175543448131E-5 54-55 2.785877717240655E-5 2.785877717240655E-5 0.0 1.4603710287661376 5.57175543448131E-5 56-57 2.785877717240655E-5 2.785877717240655E-5 0.0 1.6954433905469042 5.57175543448131E-5 58-59 2.785877717240655E-5 2.785877717240655E-5 0.0 1.9503790604515965 5.57175543448131E-5 60-61 2.785877717240655E-5 2.785877717240655E-5 0.0 2.222308584431457 5.57175543448131E-5 62-63 2.785877717240655E-5 2.785877717240655E-5 0.0 2.4954360358297305 5.57175543448131E-5 64-65 2.785877717240655E-5 2.785877717240655E-5 0.0 2.788468583517689 6.964694293101638E-5 66-67 2.785877717240655E-5 2.785877717240655E-5 0.0 3.1225092512034296 8.357633151721966E-5 68-69 2.785877717240655E-5 2.785877717240655E-5 0.0 3.4993827887917455 8.357633151721966E-5 70-71 2.785877717240655E-5 2.785877717240655E-5 0.0 3.9058841359029155 8.357633151721966E-5 72-73 2.785877717240655E-5 2.785877717240655E-5 0.0 4.311786519304879 9.750572010342292E-5 74-75 2.785877717240655E-5 2.785877717240655E-5 0.0 4.721171249853393 1.114351086896262E-4 76-77 2.785877717240655E-5 2.785877717240655E-5 0.0 5.178500935915618 1.114351086896262E-4 78-79 2.785877717240655E-5 2.785877717240655E-5 0.0 5.6865057376544526 1.114351086896262E-4 80-81 2.785877717240655E-5 2.785877717240655E-5 0.0 6.234585390355793 1.114351086896262E-4 82-83 2.785877717240655E-5 2.785877717240655E-5 0.0 6.7934046016570955 1.114351086896262E-4 84-85 2.785877717240655E-5 5.57175543448131E-5 0.0 7.350343345499262 1.114351086896262E-4 86-87 2.785877717240655E-5 5.57175543448131E-5 0.0 7.9373974274647985 1.2536449727582947E-4 88-89 5.57175543448131E-5 5.57175543448131E-5 0.0 8.580378004603942 1.3929388586203276E-4 90-91 5.57175543448131E-5 5.57175543448131E-5 0.0 9.277418538846138 1.3929388586203276E-4 92-93 5.57175543448131E-5 5.57175543448131E-5 0.0 9.991271845111886 1.671526630344393E-4 94-95 5.57175543448131E-5 5.57175543448131E-5 0.0 10.696753588837321 1.671526630344393E-4 96-97 5.57175543448131E-5 5.57175543448131E-5 0.0 11.420998218988375 1.8108205162064257E-4 98-99 5.57175543448131E-5 5.57175543448131E-5 0.0 12.200528592438069 2.228702173792524E-4 100-101 5.57175543448131E-5 5.57175543448131E-5 0.0 13.039119573493265 2.228702173792524E-4 102-103 5.57175543448131E-5 5.57175543448131E-5 0.0 13.898493202319077 2.228702173792524E-4 104-105 5.57175543448131E-5 5.57175543448131E-5 0.0 14.743463843346753 2.228702173792524E-4 106-107 5.57175543448131E-5 5.57175543448131E-5 0.0 15.592752594836153 2.228702173792524E-4 108-109 5.57175543448131E-5 5.57175543448131E-5 0.0 16.4803610943262 2.228702173792524E-4 110-111 5.57175543448131E-5 5.57175543448131E-5 0.0 17.42279566729154 2.5072899455165894E-4 112-113 5.57175543448131E-5 5.57175543448131E-5 0.0 18.399733335784905 2.5072899455165894E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATGGGG 10025 0.0 31.071215 4 GTACATG 15520 0.0 30.639988 1 GGTATCA 6345 0.0 30.523132 1 GTATCAA 8680 0.0 29.589317 1 TACATGG 15895 0.0 29.22842 2 ACATGGG 15640 0.0 28.829052 3 TCAACGC 9685 0.0 25.459017 4 ATGGGGA 5235 0.0 24.91543 5 TATCAAC 10225 0.0 24.640432 2 AACGCAG 10025 0.0 24.640192 6 CAACGCA 9960 0.0 24.63649 5 ATCAACG 10215 0.0 24.139776 3 ACGCAGA 11330 0.0 21.589865 7 CGCAGAG 11765 0.0 20.63782 8 ATGGGGG 5360 0.0 19.334166 5 GCAGAGT 13315 0.0 17.430626 9 AGAGTAC 11385 0.0 16.751755 10-11 GAGTACA 11610 0.0 16.32196 1 CAGAGTA 12610 0.0 16.18618 10-11 ATGGGGC 4110 0.0 15.940127 5 >>END_MODULE