FastQCFastQC Report
Mon 15 Aug 2016
SRR2920947_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920947_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3589533
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70680.1969058370545695No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58920.1641439150998194No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42810.11926342507507244No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82100.039.5349161
GTATCAA121650.038.35481
CATGGGG96350.030.7815364
TCAACGC147250.030.6163374
CAACGCA149600.030.1752095
AACGCAG153850.029.480446
GTACATG160350.029.4700781
TATCAAC154650.029.4598312
ATCAACG153800.029.4286233
TACATGG162600.028.4223462
ACATGGG160550.027.7833213
ACGCAGA172100.026.3112267
CGCAGAG179650.025.2336658
AGTACTT107800.022.87963712-13
GCAGAGT197500.022.5639789
GAGTACT101400.022.4458912-13
ATGGGGA53200.022.2768695
AGAGTAC177950.021.90168810-11
GTACTTT110400.020.61606414-15
ATGGGGG53300.020.223865
Produced by FastQC (version 0.11.5)